• 제목/요약/키워드: 4-Hydroxybenzaldehyde (4-HBD)

검색결과 1건 처리시간 0.016초

4-Hydroxybenzaldehyde, One of Constituents from Gastrodiae Rhizoma Augments Pentobarbital-induced Sleeping Behaviors and Non-rapid Eye Movement (NREM) Sleep in Rodents

  • Choi, Jae Joon;Kim, Young-Shik;Kwon, Yeong Ok;Yoo, Jae Hyeon;Chong, Myong-Soo;Lee, Mi Kyeong;Hong, Jin Tae;Oh, Ki-Wan
    • Natural Product Sciences
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    • 제21권3호
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    • pp.219-225
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    • 2015
  • In the previous experiments, we reported that ethanol extract of Gastrodiae Rhizoma, the dried tuber of Gastrodia ElataBlume (Orchidaceae) increased pentobarbital-induced sleeping behaviors. These experiments were undertaken to know whether 4-hydroxybenzaldehyde (4-HBD), is one of the major compounds of Gastrodiae Rhizoma increases pentobarbital-induced sleeping behaviors and changes sleep architectures via activating GABAA-ergic systems in rodents. 4-HBD decreased locomotor activity in mice. 4-HBD increased total sleep time, and decreased of sleep onset by pentobarbital (28 mg/kg and 40 mg/kg). 4-HBD showed synergistic effects with muscimol (a GABAA receptor agonist), shortening sleep onset and enhancing sleep time on pentobarbital-induced sleeping behaviors. On the other hand, 4-HBD (200 mg/kg, p.o.) itself significantly inhibited the counts of sleepwake cycles, and prolonged total sleep time and non-rapid eye movement (NREM) in rats. Moreover, 4-HBD increased intracellular Cl levels in the primary cultured cerebellar cells. The protein levels of glutamic acid decarboxylase (GAD) and GABAA receptors subunits were over-expressed by 4-HBD. Consequently, these results demonstrate that 4-HBD increased NREM sleep as well as sleeping behaviors via the activation of GABAA-ergic systems in rodents.