• Parasites, Hosts and Diseases
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• 제30권4호
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• pp.355-358
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• 1992

Oxygen radical은 생물체의 산소대사 과정의 부산물로 생성되어 세포내 여러 성분을 불환성화시키거나 미생물에 대한 방어기전으로 작용한다. 기생충에 존재하는 antioxidant enzyme은 숙주의 방어기전에서 유리하는 oxygen radical의 독성을 제거하므로 이 효소의 활성도는 기생충의 생존에 영향을 미친다고 생각하고 있다. 폐흡충은 피낭유충이 종숙주에 침입한 다음 숙주내 조직이행 시기를 거쳐서 폐에 도달하여 성충이 되므로 이 과정에서 각 시기별 산소독성과 이에 대항하는 효소의 환성이 다를 것으로 추정하였다. 폐흡충의 피낭유충과 감염후 4주, 8주, 12주에 얻은 충체의 추출물을 조효소(조효소)로 하여 SOD, catalase, peroxidase, glutathione peroxidase의 활성도를 측정하였다. 각 효소의 비환성도(specific activity) 중 catalase는 피낭유충에서 최고치였으며, SOD 와 peroxidase는 4주 충체에서 가장 높았고, glutathione peroxidase는 8주 충체에서 높았다. 이들 4가지 antioxidant효소의 비환성도는 감염 12주인 성충에서 모두 낮게 측정되어 조직 이행시기의 충체에서 더 높은 효 소 활성도를 지니고 있음을 알 수 있었다.

• 대한치과의사협회지
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• 제15권12호
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• pp.957-962
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• 1977

The author has observed the tissue reaction of the absolute alcohol infection of rat oral mucosa. 0.5ml absolute alcohol was injected subcutaneously on the mucobuccal fold of rat. And the rats were sacrifieced at intervals of one day, 3rd, 1 week, 2 week and 4 week after alcohol injection. The microscopic tissue sections were made and stained with hematoxylin and eosin. The results were are as follows; 1. Degeneration and shrinkage of fibroblasts and coagulative necrosis were observed one day to and three day after alcohol injection. 2. Although coagulative necrosis and tissue degeneration occurred, the inflammatory infiltration was not prominent especially there were scarcely any polymorphonuclear leukocytes in that field. 3. Granulation tissue with moderate small round cell infiltration were replaced the necrotic area at one week after injection and the fibroblast proliferate into the granulation tissue at two week group. 4. At four week after injection, the damaged area recovered by fibroblastic proliferation and collage formation, but there were

• Parasites, Hosts and Diseases
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• 제37권4호
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• pp.285-287
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• 1999

Changes in the expression level of splenocyte $IFN-{\gamma}$mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern blot. The template RNA was extracted from the splenocytes of rats infected with 20 metacercariae of P. westermani. The products of competitive RT-PCR were subjected to southern blot and enhanced chemiluminescence (ECL), and analyzed with a densitometer. In comparison with that of uninfected control rat splenocytes (value of 1), the levels of mRNA expression of $IFN-{\gamma}$had changed to 0.747 at 1 week post infection (PI), 0.00175 at 2 week PI, 0.0217 at 3 week PI, 0.194 at 4 week PI and then to 0.537 at 5 week PI. The level at 7 week PI had returned to 1.25, comparable with that of uninfected rats. These results show that, when infected with p. westermani, the levels of $IFN-{\gamma}$ mRNA of SD rat splenocytes were remarkably reduced by more than 500 times at 2 week PI and restored to normal level at 7 week PI.

• Journal of Preventive Medicine and Public Health
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• 제44권4호
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• pp.167-175
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• 2011

Objective: This study was designed to describe the trends in body mass index (BMI) during 6 years (2002-2008) and to identify associations between these trends and the amount of physical activity of South Korean career soldiers. Method: This study targeted the 40 993 (38 857 men and 2136 women) of the 58 657 career soldiers who had undergone four (2002, 2004, 2006, and 2008) biennial medical examinations conducted by the National Health Insurance Corporation; 17 664 soldiers with missing data on height, weight, and physical activity were excluded. A linear mixedregression model was used to categorize changes in BMI due to age versus those due to amount of physical activity. Results: Career soldiers experienced significant increases in BMI compared with baseline data gathered in 2002. The increases in each age group were as follows: men aged 20-29: 1.16, men aged 30-39: 0.61, men aged 40-49: 0.05, women aged 20-29: 0.35, women aged 30-39: 0.30, women aged 40 -49: 0.26, and women aged 50-59: 0.21. However, men aged 50 or older showed significant decreases (as high as 0.5) in BMI compared with baseline data obtained in 2002. They also experienced significant decreases in BMI compared with those who reported no physical activity. The differences between baseline and final BMIs were: 0.02 for men exercising 1-2 times per week, -0.07 for men exercising 3-4 times per week, -0.19 for men exercising 5-6 times per week, -0.21 for men exercising seven times per week, -0.05 for women exercising 1-2 times per week, -0.19 for women exercising 3-4 times per week, -0.30 for women exercising 5-6 times per week, and -0.30 for women exercising seven times per week. Conclusions: Obesity in South Korean career soldiers increased markedly between 2002 and 2008, and our data showed that the amount of physical activity was inversely related to increases in BMI. Policies to prevent obesity are needed to reduce this trend.

• Parasites, Hosts and Diseases
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• 제54권2호
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• pp.163-171
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• 2016

As most infections by the helminth parasite elicit the recruitment of $CD4^+CD25^+Foxp3^+$ T ($T_{reg}$) cells, many scientists have suggested that these cells could be used for the treatment of immune-mediated inflammation and associated diseases. In order to investigate the distribution and alteration of activated $T_{reg}$ cells, we compared the expression levels of $T_{reg}$ cell activation markers in the ileum and gastrocnemius tissues 1, 2, and 4 weeks after infection. The number of $T_{reg}$ cells was monitored using GFP-coded Foxp3 transgenic mice. In mice at 1 week after Trichinella spiralis infection, the number of activated $T_{reg}$ cells was higher than in the control group. In mice at 2 weeks after infection, there was a significant increase in the number of cells expressing Foxp3 and CTLA-4 when compared to the control group and mice at 1 week after infection. At 4 weeks after infection, T. spiralis was easily identifiable in nurse cells in mouse muscles. In the intestine, the expression of Gzmb and Klrg1 decreased over time and that of Capg remained unchanged for the first and second week, then decreased in the 4th week. However, in the muscles, the expression of most chemokine genes was increased due to T. spiralis infection, in particular the expression levels of Gzmb, OX40, and CTLA-4 increased until week 4. In addition, increased gene expression of all chemokine receptors in muscle, CXCR3, CCR4, CCR5, CCR9, and CCR10, was observed up until the 4th week. In conclusion, various chemokine receptors showed increased expressions combined with recruitment of $T_{reg}$ cells in the muscle tissue.

• 대한치과보철학회지
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• 제44권5호
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• pp.606-616
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• 2006

Purpose: This experiment examined the effect of different surface treatment on the osseointegration and stability of implants. Material and methods: In this study, 40 each of machined, SLA and RBM implants, which are the most commonly used implants, were implanted into the tibia of 20 normal rabbits using $OsseoCare^{TM}$. The rabbits were sacrificed after 1 week, 4 weeks, 8 weeks and 12 weeks for implant stability analysis, removal torque analysis, histologic and histomorphometric analysis. Result : ISQ showed significant difference between Machined and RBM at first week and at 4 weeks. There was significant difference between Machined and both SLA and RBM(p<0.05) but after 8 weeks there were no significant difference between each group. In the removal torque, RBM showd significantly higher values than SLA and Machined surface at 1st week. At 4th and 12th week, there was significant difference between Machined and SLA, RBM(p<0.05). In the bone to implant contact variable, there was no significant difference between each surface treatment method. In the Machined surface group, there was no significant difference between each time interval. but in SLA group, there were significant differences between the 1st week and 12th week and in RBM group, there were significant differences between the 1st week and 8th, 12th week and between 4th and 12th week(p<0.05). The bone area showed significantly higher values in SLA and RBM compared to Machined surface 1st and 8th week and significantly higher values in SLA than Machined surface at the 4th week(p<0.05). Conclusion: The roughened surface of implants showed positive effect in the early stages of implantation and assisted in bone formation After the bone formation stage, there was no statistical difference between Machined and roughened surface groups. In dental implantation, where initial stability is critical to the success of implants, the use of roughened surface implants should assist in reducing the healing period after implantation.

• 대한수의학회지
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• 제12권1호
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• pp.7-14
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• 1972

Hemogram and serum composition of pregnant rabbits were studied and the results obtained were as follows: 1. Erythrocyte, hemoglobin concentration and hemotocrit value of pregnant rabbits decreased at the late stage of gestation, and 1 week after delivery the values were recovered to the normal levels. Number of erythrocyte and hematocrit value were not found to be significant, but hemoglobin concentration showed significant differences at the third and fourth week of pregnancy. 2. Total leucocytes of pregnant rabbits increased continuously as gestation progresses and 1 week postpartum the values were returned to normal. Highly significant changes were observed in the third and the fourth week of pregnancy. Neutrophil was revealed highly significant rise at the third and the fourth week of gestation and 1 week postpartum, but lympocyte was decreased highly significantlly. 3. Serum calcium of pregnant rabbits revealed the tendency of decrease as gestation progresses and 1 week after delivery the value was returned to normal, and highly significant decrease was abserved at the fourth week. Serum inorganic phosphorus of pregnant rabbits showed highly significant decrease at the fourth week of gestation, but no differences were observed at the other period of gestation and 1 week postpartum. 4. Serum protein of pregnants rabbits showed the tendency of decrease continuously pregnancy progresses, and 1 week after delivery showed the normal values. Highly significant decrease was found at the fourth week of gestation. the levels of serum lipids in pregnant rabbits was unchanged untill the third week of pregnancy, but at the fourth week and 1 week postpartum, the rise was highly significant. The level of total serum cholesterol in pregnants rabbis was shown the tendency of decrease untill the end of the second week of gestation and significantly dropped at the third week, thereafter, the value was rose significantly at the fourth week of gestation and 1 week postpartum.

• Journal of Korean Biological Nursing Science
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• 제4권1호
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• pp.5-23
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• 2002

Purpose: To determine the effect of the Fall Prevention Program(EPP) on gait, balance and muscle strength in elderly women at a nursing home. Method: The subjects of this consisted of 38 elderly women between the ages of 70 to 89 years living at a nursing home located in Seoul. Each of the experimental group and control group was composed of 19 subjects. The subjects in experimental group have participated in FPP for the 8 weeks which consisted of exercise, education and foot care. They started to exercise for 40 minutes per session, 3 sessions a week during the 1st week at 40% of age adjusted maximum heart rate. From the 2nd week to the 4th week, they increased the duration of exercise to 50 minutes per session and the intensity to 60% of age-adjusted maximum heart rate. They participated in 50 minutes at 60% of age-adjusted maximum heart rate from the 5th week to the 8th week. Each exercise session consisted of 10 minutes of warming-up exercise, 30 minutes of conditioning exercise and 10 minutes of cooling-down exercise. They participated in education for 20 minutes per week from the 1st week to the 4th week. Then they participated in a 30-minute foot care program per week from the 5th week to the 8th week. Gait, balance and muscle strength for each subject were measured before and after FPP. Gait was evaluated by step length, step width, gait speed and walking distance. Balance was measured by the duration of standing on one leg with their eyes closed and open each, and a get-up and go test. Grip strength was measured by hand dynamometer. Hip extensor and flexor strength, knee extensor and flexor strength and ankle plantarflexor and dorsiflexor strength were measured by manual muscle tester. Data was analyzed using SPSS form Windows. t-test and Chi square test were utilized as a homogeneity test. Repeated measure ANOVA was used to test the effect of FPP. Result: 1) Step width significantly decreased, and step length, gait speed and walking distance significantly increased in the experimental group compared with the control group after FPP(p<0.005). 2) There was no significant change in standing time on one leg with their eyes closed after FPP. The standing time on leg with their eyes open and the time of "get-up and go" significantly decreased in the experimental group compared with the control group after FPP(p<0.005). 3) Muscle strength-grip strength, hip extensor and flexor strength-significantly increased in the experimental group compared with the control group after FPP(p<0.005). 4) There was no significant difference of frequency of fall between the experimental group and control group during the period of FPP. Conclusion: These results suggest that FPP can increase gait, balance and muscle strength of elderly women at a nursing home.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2004년도 International Conference Global Environmental Problems and their Health Consequences
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• pp.73-86
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• 2004

The purpose of this study is the identification of (N-2-hydroxy-3-butenyl) valine(HBVal adduct) and (N-2,3,4-trihydroxy-butyl)valine(THBVal adduct)with mice inhalation exposure with 1,3-butadiene for 3 weeks($6\;hr/day\;{\times}\;5\;days/week$). Body weights were significantly lower from 4 or 9 exposure post-day in 1000 or 500ppm inhalation group than in control. The levels of HBVal adducts are 1.8, 3.7 and 6.2 pmol/mg globin in $1^{st}$, $2^{nd}$ , and $3^{rd}$ week for 500 ppm 1,3-butadiene(BD), and 5.7, 7.4 and 16.0 pmol/mg globin in $1^{st}$, $2^{nd}$ , and $3^{rd}$ week for 1000 ppm BD inhalation exposure. The levels of THBVal adducts are 32.0, 42.0 and 55.0 pmol/mg globin in $1^{st}$, $2^{nd}$ , and $3^{rd}$ week for 500 ppm BD, and 67.8, 72.7 and 83.5 pmol/mg globin in $1^{st}$, $2^{nd}$ , and $3^{rd}$ week for 1000 ppm BD inhalation exposure. Their ratios of THBVal and HBVal adducts are higher at earlier exposure and lower concentration. They are17.8, 11.4 and 8.87 in $1^{st}$, $2^{nd}$ , and $3^{rd}$ week for 500 ppm BD, and 11.9, 9.8 and 5.2 in $1^{st}$, $2^{nd}$ , and $3^{rd}$ week for 1000 ppm BD inhalation exposure. In conclusion, THBVal and HBVal adducts are a important hemoglobin adduct for monitoring of BD exposure, and the latter is more biomarker than the other.

• 대한치과교정학회지
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• 제30권1호
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• pp.9-17
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• 2000

본 연구는 발치 후 인접치 이동을 위한 교정력 적용 시 적용 시기에 따른 치아이동 양상을 비교$\cdot$분석하기 위하여 시행되었다. 성견을 실험대상으로 상악 좌우 제 3절치를 3주 간격으로 각각 발거 하고, 양 견치 를 고정 원으로 제 2절치의 치체이동을 위한 장치를 장착하였다. 100 gm의 지속적인 힘을 내는 견인 스프링을 사용하여 발치 후 4주와 1주부터 좌우 제 2절치를 각각 12주간 동시에 견인하였다. 2주 간격으로 디지털 캘리퍼스를 이용하여 이동거리를 측정$\cdot$비교하고 실험에 사용된 좌우 제 2절치의 조직소견을 관찰하여 다음과 같은 결론을 얻었다. 1. 교정력 적용 후 처음 2주간에는 발치 후 4주에 견인한 치아가, 그 다음 2주간에는 발치 후 1주에 견인한 치아가 빠르게 이동하는 양상을 보였다. 2. 12주간의 실험기간 동안 견인 4주와 6주 사이에 가장 빠르게 이동하는 경향을, 이 후에는 이동 속도가 감소하는 양상을 보였다. 3. 실험기간 동안 이동한 전체 거리는 발치 후 4주에 비해 1주에 견인한 경우에서 크게 나타나는 경향을 보였다. 4. 발치 후 4주에 견인한 경우에 비하여 1주에 견인한 경우에서 유해한 조직소견이 나타나지 않았다. 이상의 결과는 발치 후 교정력 부여 시 조기 적용이 유리함을 시사하였다.