• Maxillofacial Plastic and Reconstructive Surgery
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• 제11권1호
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• pp.203-220
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• 1989

Ever since the expression of new tumor-specific antigens was reported during malignant transformation, studies on separation, purification and characterization of these proteins have been so activated recently. Following experiment was performed to observe tumor-specific antigens by implanting DMBA pellet into submaxillary gland of rat for inducing salivary gland tumor. After dividing 280 rats into 2 groups, in control group, sham operation was performed on right submaxillary gland and, in experimental group, DMBA pellet (5mg) was implanted into right submaxillary gland. Then proteins from excised submaxillary gland by killing 10 rats every two weeks for 28 weeks were extracted with 3M KCl, and SDS-PAGE and PAS-staining were carried out for biochemical examination. The obtained results were summarized as follows; 1) At 12th week since implantation of DMBA pellet, tumor mass formation was inspected. And dysplasia at 6th week and invasive epidermoid carcinoma at 10th week were observed by microscope. 2) In control group, the weight ratio of both submaxillary glands had no any change, however, in experimental group, the ratio was increased remarkably. And at 28th week after DMBA implantation, there was more than 15 times of differences in weight between control and experimental group. 3) There was no DMBA remnant after 22nd experimental week. 4) In the SDS-PAGE, high molecular protein bands (more than 100 kd) were appeared much, and new prominent protein bands (66, 48, 41.5, 39, 37, 37.5 kd) were appeared after 4th week since DMBA implantation. However, 38, 27, 22kd protein bands were disappeared. 5) In PAS-staining, high molecular proteins were proteins were all glycoproteins and 37.5kd protein was proved as to be glycoprotein. And 38kd glycoprotein was disappeared after 4th week since DMBA implantation.

• 대한물리치료과학회지
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• 제10권2호
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• pp.73-85
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• 2003

The purpose of the present study was to the investigate the effect of sports massage, kaltenborn-evjenth orthopedic manipulative therapy and electrical therapy, general exercise on the limitation of range of motion(ROM) and on the pain(VAS) in patients with frozen shoulder. Fiftheen frozen shoulder patients between 50 and 60 years of age(females) were selected and were divided equally by random distribution into A group(sports massage, kaltenborn-evjenth orthopedic manipulative therapy, N=7)and B(electrical therapy, general exercise therapy, N=7) group. The results obtained were as follows : 1) The range of motion between two groups are significantly different in the treatment times, the ROM of A group increase in after-treatment(2 week 4 week) in comparison with ROM in before-treatment, it is significant increase. And it is significant difference in B group. 2) The pain level between two groups are not significantly different in the treatment times, the pain level of A group decrease in after-treatment(2 week, 4 week) in comparison with before-treatment, it is significant decrease. And it is significant difference in B group. Although the pain level of the A group decrease in after 2 week treatment in comparison with before-treatment but it is not significant decrease. Although the pain level of the B group decrease in after 4 week treatment in comparison with after 2 week treatment, but it is not significant decrease. 3) The A group is more effective in increasing the ROM and decreasing the pain level than B group during treatment times. The results showed that both A group method and B group method are effective ROM increase and pain reduce, but A group method is superior to B group method in ROM increase and pain reduce.

• Fisheries and Aquatic Sciences
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• 제8권3호
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• pp.128-131
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• 2005

The effects of alternate-week feeding strategies on growth and feed efficiency ratio of juvenile Nile tilapia were investigated in a recirculating aquaculture system. Twenty fish initially weighing 25.5 g were randomly distributed into each of 18 tanks to conduct three replicates for each of six different feeding strategies. Weight gain of fish in the control that were fed daily for 6-week feeding trial was significantly higher than those of fish subjected to various alternate-week feeding regimes. Weight gain of fish that were starved for 3 weeks and then fed daily for 3 weeks (3WS+3WF) was not significantly different from that of fish, starved and fed daily for alternate 1-week period during the trial (1WS+1WF), but was significantly higher than those of fish starved for 2 weeks, and fed for 2 weeks (2WS+2WF); fish starved for 4 weeks and fed for 2 weeks (4WS+2WF); and fish fed for 5 weeks and fed for 1 week (5WS+1WF.) The amount of feed supplied to fish in the groups of 1WS+1WF and 3WS+3WF was significantly lower than that fed to fish in the control group, but significantly higher than the amount feed supplied to fish in the other three groups. Feed efficiency ratio for fish in the control group did not differ from that for fish in the groups of 1WS+1WF, 2WS+2WF and 3WS+3WF, but was significantly higher than that for fish in the groups of 4WS+2WF and 5WS+1WF. In conclusion, juvenile Nile tilapia that were subject to starvation for 1 to 5 weeks did not exhibit compensatory growth sufficient to attain the same weight as fish fed daily for 6 weeks. In addition, fish subjected to starvation exhibited low feed efficiency ratio compared to fish fed daily, which were probably attributable to poor weight gain.

• 대한통합의학회지
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• 제10권4호
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• pp.165-173
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• 2022

Purpose : This study was conducted to apply a 12-week resistance exercise program to obese elderly people with sarcopenia and verify the risk factors of sarcopenia and metabolic syndrome as well as the effects of this program on improving muscle function, and thus to serve as basic data for preventing and improving sarcopenia. Methods : Forty elderly people aged 65 or older were recruited and underwent dual energy x-ray absorptiometry. Based on the criteria of appendicular skeletal muscle mass (ASM/Height²: less than 5.4 kg/m²) and body fat percentage (at least 30 % for women and 25 % for men), 18 obese elderly people with sarcopenia were finally selected after excluding 22 elderly people who did not meet the criteria. Variables related to sarcopenia, metabolic syndrome, and muscle function were measured before the 12-week resistance exercise program. Results : The 12-week resistance exercise program significantly increased the bone density and muscle mass and decreased the fat mass and fat percentage in obese elderly with sarcopenia. The 12-week resistance exercise program significantly increased the HDL-C and decreased the LDL-C and waist circumference in obese elderly people with sarcopenia (p<.05). The 12-week resistance exercise program significantly increase grip strength, static balance, and 6-minute walking in obese elderly people with sarcopenia (p<.05). Conclusion : Accordingly, resistance exercise is considered a way to reduce the exorbitant medical expenses of patients who are bedridden for long and improve the lowered quality of individuals in a super-aged society.

• 한국양식학회지
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• 제22권1호
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• pp.11-15
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• 2009

Effect of fasting and refeeding on growth and blood chemistry of juvenile olive flounder Paralichthys olivaceus L. was investigated when fish achieved compensatory growth. Fish were fed the experimental diet for 6 days a week. Five treatments in triplicate were prepared: C, S1, S2, S3 and S4. Fish in the control group (C) were hand-fed to apparent satiation twice a day. Fish in treatments of S1, S2, S3 and S4 experienced 1, 2, 3 and 4 weeks of starvation and were then hand-fed to satiation twice daily during the remaining 7, 6, 5 and 4 weeks of the experiment, respectively. Weight gain of fish in C, S1 and S2 were higher than those of fish in S3 and S4. A significant difference in plasma total protein, glucose, triglyceride, $T_3$ and $T_4$ was observed in between starved and refed fish for the rest periods of the feeding trial. Plasma total protein and $T_3$ of flounder decreased with week of fasting and following correlationships were obtained; Y (Total protein) = -0.13X (week of fasting) + 1.54, $R^2=0.9792$ and $Y(T_3)=-11.48X$ (week of fasting) + 79.57, $R^2=0.8822$, respectively.

• Journal of Periodontal and Implant Science
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• 제34권3호
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• pp.475-487
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• 2004

Purpose: This study was aimed to evaluate the effect of the deproteinated bovine bone powder (DBBP) coated with calcium phosphate (Ca-P) on osseous regeneration in the calvarial bone defect of rat. Materials and Methods : The DBBP (Control group, n=6) and the Ca-P coated DBBP (Experimental group, n=6) were grafted in the critical sized calvarial bone defect (8 mm) of rat weighing 250 g. The animals were sacrificed at 1, 4 week. The biopsy specimens were decalcified with 5%formaldehyde and embedded in paraffin. The rats were sacrificed at 8 week received tetracycline (1 week), calcein blue (4 week), and alizarin red (7 week), and the biopsy specimens were taken. The specimens were embedded in methylmethacrylate and ground to 10 ${\mu}m$ thin sections were made. All of the specimens were stained with H & E and Masson's trichrome and examined under light microscope. The specimens at 8 week were examined under fluorescent microscope. Results : In the Control group, the grafted DBBP was surrounded with connective tissue, and osteoblasts were observed partially around the grafted particles at 1 week. At 4 week, some osteoid was observed and, new bone formation was observed at the periphery of grafted materials at 8 week, In the Experimental group, some osteoid was seen at the periphery of the grafted Ca-P coated DBBP at 1 week, and osteoblast and newly formed bone were observed around the grafted materials. At 8 week, newly formed bone was observed at the periphery of the grafted materials. Conclusion: These results suggest that Ca-P coated DBBP group was more and faster than DBBP group in new bone formation and Ca-P could contribute to enhance bone formation in the critical sized calvarial bone defect of rat.

• 대한한방내과학회지
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• 제15권1호
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• pp.45-59
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• 1994

The present experiments were designed to investigate the effect of Ijntang, Sagoonjatang, and Yuggoonjatang on the hyperlipidemia induced rabbit. In order to control the precise chemical and physical condition, the experimental rabbits were supplied with calory limited food. The Hyperlipidemia rabbits were induced by oral administration of cholesterol (250mg/kg) for 4 weeks. The results were as followings : 1. The hyperlipidemia (control) group increased the concentration of serum glucose to $204.1{\pm}2.63\;mg/dl$ at the 4th. week, however, the other drug administrated groups such as Ijintang, Sagoonjatang, and Yuggoonjatang decreased significantly. 2. The control group increased the concentration of serum totalcholesterol to $299.0{\pm}4.18\;mg/dl$ at the 4th. week, however, the other drug administrated groups Ijintang, Sagoonjatang, and Yuggoonjatang decreased significantly. 3. The control group increased the concentration of serum total lipid to $429.1{\pm}1.72\;mg/dl$ at the 4th. week, however, the other drug administrated groups such as Ijintang, Sagoonjatang, and Yuggoonjatang decreased significantly. 4. The control group increased the concentration of serum triglyceride to $149.3{\pm}3.01\;g/dl$ at the 4th. week, however, the other drug administrated groups such as Ijintang, Sagoonjatang, and Yuggoonjatang decreased significantly. 5. The control group increased the concentration of serum HDL-cholesterol to $83.0{\pm}1.09$\;mg/dl$ at the 4th. week, and, the other drug administrated groups such as Ijintang, Sagoonjatang, and Yuggoonjatang increased significantly. 6. The lipid deposition to the aortic endothelium decreased more at the Yuggoonjatang group than the other groups. According to the above experimental results, Yuggoonjatang is assumed to have a more curative effect against hyperlipidemia than the other drug such as Ijintang and Sagoonjatang.

• Journal of Ginseng Research
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• 제18권1호
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• pp.60-65
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• 1994

Fresh ginseng of same grade was stored under the 4$\pm$1$^{\circ}C$ and 87~92% RH for 10 weeks. During the storage, an aliquot amount of the ginseng was drawn, freeze dried and chemical constituents and physicochemical parameters were measured. After 10 weeks of storage drying rate and shrinkage of ginseng were 1520% and 9.04%, respectively, mold growth was seen at week 5 and observed for 51.2% of the ginseng week 10. Amylase activity level was elevated at the early stage of storage and decreased to 5% of initial value at week 5. At week 5, the elevated amylase activity was inconcomitant with the appearance of the mold growth. Crude protein contents were increased and decreased, respectively 5 week post storage. No significant changes in crude fat, crude fiber, ash, total sugar, n-butanol extract and ginsenoside were observed. The content of water-extractable substance showed maximum at week 7 to 8. The value of pH was slightly elevated and reducing sugar was increased during the storage. Key words Ginseng storage, physicochemical properties, drying rate, shrinkage, amylase activity.

• 대한정형외과 초음파학회지
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• 제2권2호
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• pp.79-84
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• 2009

목적: 주관절 내상과염 및 외상과염은 주관절 통증의 가장 흔한 원인 중의 하나로 이에 대한 치료방법으로 최근 체외충격파 치료가 임상에서 점점 많이 시도되고 있다. 이에 본 연구에서는 내, 외상과염에 대한 체외충격파 치료를 시행한 후 그 임상적 결과를 알아보고자 하였다. 대상 및 방법: 2005년 11월부터 2009년 1월까지 본원에서 주관절 내, 외상과염으로 진단받은 환자 중 72명 74예(남자 11명, 여자 61명)를 대상으로 하였으며 평균 연령은 52.4세였다. 모든 환자는 타 병원에서 국소 주사, 물리 치료, 약물치료, 주관절 석고 고정 등의 보존적 치료방법을 시행받았으나 증상이 호전되지 않아 본원을 방문한 경우로 치료 효과의 판정은 통증의 개선 정도를 통해 평가하였는데 휴식 시와 작업 시의 통증의 정도를 각각 치료 전과 치료 후 6주, 12주, 24주에 visual analogue scale (VAS)를 이용하여 측정하고 이와 함께 통증으로 수면 중 깨어나는 횟수를 조사하여 이를 같은 방식으로 치료 전, 후를 비교하여 평가하였다. 결과: 치료 24주 째 최종 추시에서 휴식시 통증의 경우 우수 70예, 양호 4예였으며 불량은 없었고 작업시 통증의 경우 우수 42예, 양호 14예, 보통 8예였으며 불량은 없었다. 이에 따라 작업시 VAS를 바탕으로 한 치료 성공률은 약 89.2%로 확인되었다. 휴식시 통증의 VAS 평균값은 치료 전 4.60에서 치료 6주째 2.01, 12주째 0.43, 24주째 0.16으로 개선되었고 작업시 통증의 경우 평균값이 치료 전 7.20에서 치료 6주째 4.05, 12주째 2.01, 24주째 0.36으로 유의하게 개선되었다. 수면 중 깨어나는 빈도 역시 치료 전 1주일에 평균 9.64회에서 치료 6주, 12주, 24주가 되면서 각각 1.21회, 0.08회, 0.09회로 유의한 치료 효과를 보여주었다. 결론: 체외충격파를 이용한 주관절 내, 외상과염의 치료는 보존적 치료에 반응하지 않는 환자들에서 통증의 감소와 근력 증진을 위한 효과적인 치료법으로 사료된다.

• 대한한방내과학회지
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• 제35권3호
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• pp.223-243
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• 2014

Objectives: To provide information on the safety of GST (GamiSasangja-tang; CnidiiFructus, Sophora Root, Angelica Gigas Root, Clematidis Radix, Stemonae Radix, Spirodelae Herba), we carried out a 13-week repeated oral dose toxicity and a 4-week recovery test of GST in Sprague-Dawley rats. Methods: Female and male rats were treated with GST at oral doses of 1,250, 2,500, and 5000 mg/kg. The GST was administered for 13 weeks. Mortality, clinical signs, body weight changes, food consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights and histological markers were monitored during the study period. The rats were then monitored for 4 extra weeks to determine recovery time after the study period. Results: We found no mortality or abnormalities among clinical signs, body weight, food consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights or histological markers in any of the rats tested. Conclusions: The no-observed adverse effects level (NOAEL) is considered as over 5000 mg/kg for male and female rats.