• 대한화학회지
• /
• 제35권6호
• /
• pp.720-724
• /
• 1991

액상 액체 크로마토그래피(HPLC)에 의해 구절초 중 chlorogenic acid (CA), 3,4-o-dicaffeoyl quinic acid (3,4-DCQA), 4,5-o-dicaffeoyl quinic acid (4,5-DCQA)와 linarin의 동시 정량분석법이 새로히 확립되었다. 시료를 20ml 메탄올로 4시간 추출하고 그 추출물을 Sep-Pak $C_18$ cartridge와 용출액으로 4 ml 메탄올-물(1:1)을 사용해서 분리, 정제하였다. HPLC 정량법으로 Bondapak $C_18$ 컬럼(30 cm ${\times}$ 3.9 mm i.d., 10 ${\mu}m$)과 이동상으로 메탄올 -5mM 인산수용액(30:70)의 기울기 용출방식이 사용되었다. 위 정량분석법을 이용해서 시중 구절초 중 그 함량범위는 CA에서 0.35~0.55%, 3,4-DCQA에서 0.46~0.76%, 4,5-DCQA에서 0.077~0.231% 및 linarin에서 0.16~2.72%이었다.

• 생약학회지
• /
• 제25권1호
• /
• pp.11-19
• /
• 1994

Gleditsia japonica var. koraiensis NAKAI(Leguminosae) is commonly distributed in Korea and has been used as a folk medicine in the treatment of bronchitis, neoplasm and blennorrhgia in the Orient. The aqueous acetone extract of the leaves of G. japonica was subjected to a combination of Sephadex LH-20, Cosmosil $75C_{18}-OPN$, TSK-gel Toyopearl HW 40F, Avicel cellulose, and MCI-gel CHP 20P chromatographies with various solvent systems. Twelve compounds were isolated and confirmed to be vitexin(1), isovitexin(2), orientin(3), isoorientin(4), 4-caffeoyl quinic acid(5), 5-caffeoyl quinic acid(6), 3, 5-dicaffeoyl quinic acid(7), 4, 5-dicaffeoyl quinic acid(8), caffeic acid(9), quercetin(10), isoquercitrin(11) and luteolin-7-O-glucoside(12), on the basis of chemical and spectroscopic evidences.

• 생약학회지
• /
• 제25권1호
• /
• pp.70-72
• /
• 1994

From the herb of Artemisia princeps var. orientalis o-coumaric acid and 4,5-O-dicaffeoylquinic acid were isolated and identified by chemical and spectral analysis.

• Natural Product Sciences
• /
• 제10권6호
• /
• pp.335-340
• /
• 2004

The chromatographic separation of the MeOH extract of the aerial parts from Aster hispidus (Compositae) led to the isolation of eight compounds. Their structures were established by spectroscopic methods to be ${\beta}-amyrin$ (1), oleanolic acid (2), (2R)-1, 2-O-(9Z, 12Z, $15Z-dioctadecatrienoyl)-3-O-{\beta}-D-galactopyranosyl\;glycerol$ (3), trans-phytol (4), 9, 12, 15-octadecatrienoic acid (5), kaempferol (6), 3,5-dicaffeoyl quinic acid (7), 3,4-dicaffeoyl quinic acid (8) and kaempferol-3-O-rutinoside (9). Compounds 1, $3{\sim}6$ and 9 showed non-specific moderate cytotoxicity against five human tumor cell lines $(5.44{\sim}23.51\;{\mu}g/ml)$. The other compounds were of marginal activity against tested five human cancer cell lines $(9.05{\sim}>30.0\;{\mu}g/ml)$.

• 한국자원식물학회지
• /
• 제20권3호
• /
• pp.221-225
• /
• 2007

The plant Ligularia fischeri var. spiciformis (Compositae) is a candidate for available functional foods. It has been used to treat diabetes mellitus and rheumatoid arthritis. We have reported the isolation of a new eremophilanolide named 6-oxoeremophilenolide and cytotoxic intermedeol together with the isolation of hydrophilic constituents, chlorogenic acid, 3,4-di-O-caffeoylquinic acie (3), and 5-O-[1-butyl]-3,4-di-O-caffeoylquinic acid. Compound 3 was again isolated by combination of silica gel- and ODS column chromatography for the anti-nociceptive action. Compound 3 and 4 were assayed in hot plate- and writhing tests in the rat. Although the three derivatives of caffeic acid exhibited significant anti-nociceptive effects at 10 mg/kg dose (i.p.),(activity potency: 4>3). These results suggest that compound 3 is responsible for at least rheumatoid arthritis, and caffeic acid moiety is the active moiety of dicaffeoylquinic acid.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
• /
• pp.387.3-388
• /
• 2002

The plant Ligularia fischerivar. spiciformis(Compositae) is a candidate for available functional foods. It has been used to treat diabetes mellitus and rheumatoid arthritis. We have reported the isolation of a new eremophilanolide named 6-oxoeremophilenolide and cytotoxic intermedeol together with the isolation of hydrophilic constituents. chlorgenic acid. 3.4-di-O-caffeoylquinic acid(1). and 5-O-［1-butyl］-3.4-di-O-caffeoylquinic acid. (omitted)