• Title/Summary/Keyword: 3D-frame

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Expression and DNA Sequence of the Gene Coding for the lux-specific Fatty Acyl-CoA Reductase from photobacterium phosphoreum

  • Lee, Chan-Yong;Edward A. Meighen
    • Journal of Microbiology
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    • v.38 no.2
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    • pp.80-87
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    • 2000
  • The nucleotide sequence of the luxC gene coding for lux-specific fatty acyl-CoA reductase and the upstream DNA (325bp)of the structural gene from bioluminescent bacterium, Photobacterium phosphoreum, has been deternubed. An open reading frame extending for more than 20 codons in 325 bp DNA upstream of luxC was not present in both directions. The lux gene can be translated into a polypeptide of 54 kDa and the amino acid sequences of lux specific reductases of P. phosphoreum shares 80, 65, 58, and 62% identity with those of the Photobacterium leiognathi, Vibrio fischeri, Vibrio harveyi, and Xehnorhabdus luminescenens reductases, respectively. Analyses of codon usage, showing that a high frequency (2.3%) of the isoleucine codon, AUA, in the luxC gene compared to that found in Escherichia coli genes (0.2%) and its absence in the luxA and B genes, suggested that the AUA codon may play a modulator role in the expression of lux gene in E. coli. The structural genes (luxC, D, A, B, E) of the P. phosphoreum coding for luciferase (${\alpha}$,${\beta}$) and fatty acid reductase (r, s, t) polypeptides can be expressed exclusively in E. coli under the T7 phage RNA polymerase/promoter system and identificationof the [35S]methionine labelled polypeptide products. The degree of expression of lux genes in analyses of codon usage. High expression of the luxC gene could only be accomplished in a mutant E. coli 43R. Even in crude extracts, the acylated acyl-CoA reductase intermediate as well as acyl-CoA reductrase activities could be readily detected.

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Modified Direct Torque Control using Algorithm Control of Stator Flux Estimation and Space Vector Modulation Based on Fuzzy Logic Control for Achieving High Performance from Induction Motors

  • Rashag, Hassan Farhan;Koh, S.P.;Abdalla, Ahmed N.;Tan, Nadia M.L.;Chong, K.H.
    • Journal of Power Electronics
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    • v.13 no.3
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    • pp.369-380
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    • 2013
  • Direct torque control based on space vector modulation (SVM-DTC) protects the DTC transient merits. Furthermore, it creates better quality steady-state performance in a wide speed range. The modified method of DTC using SVM improves the electrical magnitudes of asynchronous machines, such as minimizing the stator current distortions, the stator flux with electromagnetic torque without ripple, the fast response of the rotor speed, and the constant switching frequency. In this paper, the proposed method is based on two new control strategies for direct torque control with space vector modulation. First, fuzzy logic control is used instead of the PI torque and a PI flux controller to minimizing the torque error and to achieve a constant switching frequency. The voltages in the direct and quadratic reference frame ($V_d$, $V_q$) are achieved by fuzzy logic control. In this scheme, the switching capability of the inverter is fully utilized, which improves the system performance. Second, the close loop of stator flux estimation based on the voltage model and a low pass filter is used to counteract the drawbacks in the open loop of the stator flux such as the problems saturation and dc drift. The response of this new control strategy is compared with DTC-SVM. The experimental and simulation results demonstrate that the proposed control topology outperforms the conventional DTC-SVM in terms of system robustness and eliminating the bad outcome of dc-offset.

Generation of Humanoid Walking Motion Adapted to the Ground's Sliding Properties (지면의 미끄러운 정도에 따른 캐릭터의 걷기 동작 생성)

  • Lee KumHee;Song MiYoung;Cho HyungJe
    • The KIPS Transactions:PartB
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    • v.12B no.2 s.98
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    • pp.157-166
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    • 2005
  • In 3D virtual environment the description of character' s movement that has utilized the conventional key-frame technique is gradually being developed toward the application of motion control method to generate more realistic and natural motion. Even the motion control method, however, has the limitation for expression of character's motion adapted to the ground properties of virtual world. That is, the walking motions of character are not only, for the most part, so uniform simple and repeated often as to feel to be tedious, but also the unnatural motion in which the tips of the toes soak through a plane or float in the air discording with the conditions of terrain lowers the semblance of reality. This paper proposes an adaptive motion control method for human figure locomotion in virtual environments or games, in which the walking motion is dynamiccally adapted to the ground's sliding properties. We compute an optimal parameters for one cycle of walking motion adapted to the ground properties by combining the coefficient of friction and centripetal force, and therefrom we induce a set of nonskid speed corresponding to various sliding properties of the ground.

Kinematical Aspects Gliding Technique in 500-m Speed Skaters: From Start to Seven Strokes

  • Ryu, Jae Kyun;Kim, Young Suk;Hong, Sung Hong
    • Korean Journal of Applied Biomechanics
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    • v.26 no.4
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    • pp.333-341
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    • 2016
  • Objective: The purpose of this study was to assess the consistency of the gliding and push-off motion for single leg skating from the first to fourteenth steps. We hypothesized that: 1) there would be no difference in stroke trajectory, step rate, and cycle rate between the left and right steps of gliding; and 2) there would be a difference in the resultant velocity of toe push-off and the horizontal velocity of the center of mass after six step push-offs. Method: The study included five male 500-m speed skaters (mean height, $1.80{\pm}0.02m$; mean weight, $76.8{\pm}3.96kg$; record, $35.83{\pm}0.30sec$; 100-m record, <9.97 sec). Data were collected from the first to fourteenth steps (40 m) and recorded using five digital JVC GR-HD1KR video cameras (Victor Co., Japan) operating at a sampling frequency of 60 fields/sec and shutter speed of 1/500 sec. For each film frame, the joint positions were digitized using the KWON3D motion analyzer. Position data were filtered with low-pass Butterworth $4^{th}$ order at the cut-off frequency of 7.4 Hz. Results: The right toe of the skating trajectories at $2^{nd}$, $5^{th}$, and $7^{th}$ strokes differed from those of the left toe. The angles of the right and left knee demonstrated unbalanced patterns from the flexion and extension legs. The step and cycle rates of the right and left leg differed from the start until 20 m. The resultant velocities of the toe at the push-off phase and of the body mass center diverged before the six push-offs. Conclusion: This study's findings indicate that the toe of skating trajectory on left and right sliding after push-off should maintain a symmetrical trajectory. The resultant velocity of toe push-off and horizontal velocity from the center of body need to be separated after about six step push-offs.

The Effect of the Optical Points Difference between Finished-Reading Glasses and Dispensing Reading Glasses (완성품 돋보기와 조제가공된 돋보기가 광학적 요소에 미치는 영향)

  • Shim, Young-Cheol;Yoo, Gun-Chang;Kim, In-Suk
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.3
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    • pp.65-71
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    • 2008
  • Purpose: This paper studied the effect of eyes on the comparison between the distance optical centers problem of dispensing reading glasses made by optician and finished reading glasses in the current market. Methods: The method of this study has been measured by eleven different categories from +1.00D to +4.00D. This study also separated into three groups by their optical frame size and measured optical center point (O.C) and optical center height (O.H) with 200 peoples of man and females over 40 years old without ocular disease living in Gwang-san gu, Gwang-Ju city. Results: As a result, optical center point ranged from 57 mm to 80 mm and it turned to be most common range is from 61 mm to 65 mm (64.6%). Moreover, the optical center height ranged from 1 mm to 8mm and most common ranged (23%) were 4 mm. In other words, finished reading glasses have irregular optical ranges. After observing 200 people who are over 40 years old men and women, result shows that more than 75.5% (151 people) currently use finished reading glasses. Survey of 151 people, most common error between the finished reading glasses's O.C and the wearers P.D were 4 mm (45%). Furthermore, the most common error between the finished reading glassses's O.H and the wearers O.H ranged from 3 mm to 4 mm. Astonishingly, the entire 151 people who wear finished reading glasses appeal that they feel tiredness on their eyes when they wear finished reading glasses. 53 people (35%) claimed that they feel tiredness on their eyes after 10 to 20 minutes wearing finished reading glasses. Base on the research, We conducted more experiment to find the value of prism of optical centers err because it will tell us whether the finished reading glasses are good enough to wear or not. We multiplied diopter by the difference between finished reading glasses's O.C. and wearer's P.D. Consequently, We found out that the finished reading glasses counter to the German RAL-RG 915 policy. And We also found that it is relative to the diopter of lenses. In conclusion, based on the researched that wearing finished reading glasses have a dangerous factor for our vision. Therefore optician must need to recommend correctly made dispensing reading glasses based on the optical center point.

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Assessment of Imaging Distortion in Magnetic Resonance Imaging for Stereotactic Radiosurgery: Through Phantom Study (뇌정위 방사선수술 시스템을 위한 자기공명영상의 공간적 왜곡의 측정 : 모형실험을 통한 연구)

  • 박선원;한문희;김동규;정현태;송인찬
    • Investigative Magnetic Resonance Imaging
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    • v.4 no.1
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    • pp.7-13
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    • 2000
  • Purpose : To assess the distortion of MRI with the Leksell stereotactic radiosurgery system in variable pulse sequence and imaging plane through phantom study, to find most adequate imaging plane and pulse sequence for stereotactic radiosurgery system. Materials and methods : We made the phantoms for MRI and get images in variable conditions and analyzed the image distortion using image analysis program, and statistically using paired student t-test. Results : The transeverse plane images had acceptable error ranges bless than 1.5mm) in all pulse sequence in both the analysis of fiducial marker in stereotactic G-frame and the phantom study. The coronal plane images had unacceptable large errors (more than 1.7mm) in the analysis of fiducial marker in the stereotactic G-frame, but had corrected small errors (less than 1.5mm) in the phantom study. Conclusion : We find from the phantom study that the present MR machines are adequate for stereotactic surgery system in frequently used pulse sequences, and imaging planes.

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A Content-based Video Rate-control Algorithm Interfaced to Human-eye (인간과 결합한 내용기반 동영상 율제어)

  • 황재정;진경식;황치규
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.28 no.3C
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    • pp.307-314
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    • 2003
  • In the general multiple video object coder, more interested objects such as speaker or moving object is consistently coded with higher priority. Since the priority of each object may not be fixed in the whole sequence and be variable on frame basis, it must be adjusted in a frame. In this paper, we analyze the independent rate control algorithm and global algorithm that the QP value is controled by the static parameters, object importance or priority, target PSNR, weighted distortion. The priority among static parameters is analyzed and adjusted into dynamic parameters according to the visual interests or importance obtained by camera interface. Target PSNR and weighted distortion are proportionally derived by using magnitude, motion, and distortion. We apply those parameters for the weighted distortion control and the priority-based control resulting in the efficient bit-rate distribution. As results of this paper, we achieved that fewer bits are allocated for video objects which has less importance and more bits for those which has higher visual importance. The duration of stability in the visual quality is reduced to less than 15 frames of the coded sequence. In the aspect of PSNR, the proposed scheme shows higher quality of more than 2d13 against the conventional schemes. Thus the coding scheme interfaced to human- eye proves an efficient video coder dealing with the multiple number of video objects.

Precise Orbital and Geodetic Parameter Estimation using SLR Observations for ILRS AAC

  • Kim, Young-Rok;Park, Eunseo;Oh, Hyungjik Jay;Park, Sang-Young;Lim, Hyung-Chul;Park, Chandeok
    • Journal of Astronomy and Space Sciences
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    • v.30 no.4
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    • pp.269-277
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    • 2013
  • In this study, we present results of precise orbital geodetic parameter estimation using satellite laser ranging (SLR) observations for the International Laser Ranging Service (ILRS) associate analysis center (AAC). Using normal point observations of LAGEOS-1, LAGEOS-2, ETALON-1, and ETALON-2 in SLR consolidated laser ranging data format, the NASA/GSFC GEODYN II and SOLVE software programs were utilized for precise orbit determination (POD) and finding solutions of a terrestrial reference frame (TRF) and Earth orientation parameters (EOPs). For POD, a weekly-based orbit determination strategy was employed to process SLR observations taken from 20 weeks in 2013. For solutions of TRF and EOPs, loosely constrained scheme was used to integrate POD results of four geodetic SLR satellites. The coordinates of 11 ILRS core sites were determined and daily polar motion and polar motion rates were estimated. The root mean square (RMS) value of post-fit residuals was used for orbit quality assessment, and both the stability of TRF and the precision of EOPs by external comparison were analyzed for verification of our solutions. Results of post-fit residuals show that the RMS of the orbits of LAGEOS-1 and LAGEOS-2 are 1.20 and 1.12 cm, and those of ETALON-1 and ETALON-2 are 1.02 and 1.11 cm, respectively. The stability analysis of TRF shows that the mean value of 3D stability of the coordinates of 11 ILRS core sites is 7.0 mm. An external comparison, with respect to International Earth rotation and Reference systems Service (IERS) 08 C04 results, shows that standard deviations of polar motion $X_P$ and $Y_P$ are 0.754 milliarcseconds (mas) and 0.576 mas, respectively. Our results of precise orbital and geodetic parameter estimation are reasonable and help advance research at ILRS AAC.

A Study on the Paleomagnetism of Southern Korea since Permian (페름기(紀) 이후(以後) 한국(韓國)의 고지자기(古地磁氣)에 관(關)한 연구(硏究))

  • Kim, Kwang Ho;Jeong, Bong II
    • Economic and Environmental Geology
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    • v.19 no.1
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    • pp.67-83
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    • 1986
  • Oriented hand samples were collected from Gobangsan Formation and Nogam Formation in the north of Danyang and south of Yeongchun, from Bansong Group in and around Danyang, from Nampo Group in Chungnam Coalfield, from Gyeongsang Supergroup distributed from Waegwan through Daegu to Gyeongsan and from Daegu to Goryong, and from volcanic flows in Jeongog area and Jeju Island to study the paleomagnetism of southern Korea since Permian. Stepwise alternating field and thermal demagnetization experiments were carried out to determine optimum fields and temperatures. Observed mean paleomagnetic directions are as follows: $D=331.5^{\circ}$, $I=25.1^{\circ}$, $a95=12.8^{\circ}$ for Permian, $D=325.6^{\circ}$, $I=46.1^{\circ}$, $a95=11.8^{\circ}$ for Triassic, $D=313.4^{\circ}$, $I=43.1^{\circ}$, $a95=16.0^{\circ}$ for early Jurassic, $D=41.3^{\circ}$, $I=64.6^{\circ}$, $a95=4.5^{\circ}$ for early Cretaceous, $D=28.3^{\circ}$, $I=58.1^{\circ}$, $a95=2.3^{\circ}$ for late Cretaceous, $D=2.0^{\circ}$, $I=55.8^{\circ}$, $a95=6.6^{\circ}$for Quaternary. To describe the tectonic translocation of southern Korean block, northern Eurasian continental block was used as a reference frame. For each age since Permian the expected northern Eurasian field directions in terms of paleolatitude and declination were calculated. The paleolatitudes of Permian ($13.2^{\circ}N$) and early Jurassic ($25.1^{\circ}N$) obtained from the study area are quite different from those of Permian ($66.0^{\circ}N$) and early Jurassic ($68.1^{\circ}N$) which are expected for northern Eurasia. The declinations of Permian ($331.5^{\circ}$) and early Jurassic ($313.4^{\circ}$) are also quite different from those of the Permian ($56.6^{\circ}$) and the early Jurassic ($47.5^{\circ}$) expected for northern Eurasia. The Cretaceous paleolatitude is similar to the expected within error limit, but the declination for the same period is significantly different from that of the expected for the northern Eurasia. From the above evidences it is suggested that the south Korean land mass had moved from low latitude in Permian to north and sutured to northern continental block since early Jurassic. The relative rotations of early Cretaceous($27.4^{\circ}$) and late Cretaceous($10.8^{\circ}$) to northern Eurasian continent reveal that the Korean land mass might be rotated clockwise in two different times, probably in late Early Cretaceous and in Tertiary.

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The Usefulness of LEUR Collimator for 1-Day Basal/Acetazolamide Brain Perfusion SPECT (1-Day Protocol을 사용하는 Brain Perfusion SPECT에서 LEUR 콜리메이터의 유용성)

  • Choi, Jin-Wook;Kim, Soo-Mee;Lee, Hyung-Jin;Kim, Jin-Eui;Kim, Hyun-Joo;Lee, Jae-Sung;Lee, Dong-Soo
    • The Korean Journal of Nuclear Medicine Technology
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    • v.15 no.1
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    • pp.94-100
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    • 2011
  • Purpose: Basal/Acetazolamide-challenged brain perfusion SPECT is very useful to assess cerebral perfusion and vascular reserve. However, as there is a trade off between sensitivity and spatial resolution in the selection of collimator, the selection of optimal collimator is crucial. In this study, we examined three collimators to select optimal one for 1-day brain perfusion SPECT. Materials and Methods: Three collimators, low energy high resolution-parallel beam (LEHR-par), ultra resolution-fan beam (LEUR-fan) and super fine-fan beam (LESFR-fan), were tested for 1-day imaging using Triad XLT 9 (TRIONIX). The SPECT images of Hoffman 3D brain phantom filled with 99mTc of 170 MBq and a normal volunteer were acquired with a protocol of 50 kcts/frame and detector rotation of 3 degree. Filterd backprojection (FBP) reconstruction with Butterworth filter (cut off frequencies, 0.3 to 0.5) was performed. The quantitative and qualitative assessments for three collimators were performed. Results: The blind tests showed that LESFR-fan provided the best image quality for Hoffman brain phantom and the volunteer. However, images for all the collimator were evaluated as 'acceptable'. On the other hand, in order to meet the equivalent signal-to-noise ratio (SNR), total acquisition time or radioactivity dose for LESFR-fan must have been increased up to almost twice of that for LEUR-fan and LEHR-par. The volunteer test indicated that total acquisition time could be reduced approximately by 10 to 14 min in clinical practice using LEUR-fan and LEHR-par without significant loss on image quality, in comparison with LESFR-fan. Conclusion: Although LESFR-fan provides the best image quality, it requires significantly more acquisition time than LEUR-fan and LEHR-par to provide reasonable SNR. Since there is no significant clinical difference between three collimators, LEUR-fan and LEHR-par can be recommended as optimal collimators for 1-day brain perfusion imaging with respect to image quality and SNR.

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