• Journal of Plant Biotechnology
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• 제4권3호
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• pp.123-127
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• 2002

Herbicide tolerant rice (Oryza sativa L. cv. Ilpumbyeo) cell lines were selected from $\gamma$-ray-irradiated anther-derived cell cultures. The anther-derived cell clusters were small (300 to 400 ${\mu}{\textrm}{m}$ in diameter) and uniform ones that were screened by miracloth filtering. The cell suspensions were very efficient to plate one layer onto agar medium and to screen target cell lines. Herbicide tolerant cell lines were selected by 5 mg/L cyhalofop butyl (CHB) treatment by using the small cell suspensions on agar N6 medium containing 1 mg/L 2,4-D and 0.2 mg/L kinetin. Of the cell lines, one line (CHB-1) showed stable tolerance at 10 mg/L concentration after 6-month culture without herbicide suspension. Growth stability of CHB-1 was similar to that of control cell line on 10 mg/L CHB containing medium. In this experiment we established herbicide tolerant cell line selection system by using anther-derived uniform-cell suspensions with $\gamma$-ray-irradiation.

• Journal of Information Technology Applications and Management
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• 제18권2호
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• pp.1-21
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• 2011

In this era of convergence, which is characterized by the integration and combination of technology and industries, broadcasting and communications, offline and online, and devices and services, the content industry is also experiencing rapid changes including inter-genre exchange, the creation of new industries, and customized demand. Specifically, IT-based digital content industries such as the online game, e-book, mobile contents and web portal industries are no longer restricted to the boundaries of video, music and games but are being expanded into the realms of education, medicine, fashion and sports thanks to CT innovation of 3D, CG, AR/VR, VFX, etc. As such, various countries have come to recognize the convergent content industry as a new growth engine that will pick up where the IT industry left off, and are forming policies for its development accordingly. This research aims to optimize the system of content taxonomy which is currently genre-focused and unable to support technological development and convergence, and to discover and select star contents to be rigorously developed with governmental support. In this paper, 20 star contents in 8 areas were selected, and these are expected to create tremendous cultural and economic value through ongoing technological and industrial development.

• 한국식품영양과학회지
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• 제30권1호
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• pp.193-195
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• 2001

A radiation-resistant bacterium was isolated from gamma irradiated dried laver (Porphyra tenera) and its microbiological characteristics were examined. As a result of resistance test to gamma irradiation, the isolate was survived $10^{3}$ CFU/mL even at 30 kGy and significant shoulder line zone was shown until 20 kGy. The $D_{10}$ value was 11.27 kGy. The isolate was gram-positive, non-motile coccus and catalase-positive. n culture, the red-pigmented smooth colony was observed. The biochemical test in API (analytical profile index) system showed that the isolate fermented glucose and fructose as the carbon source. Therefore, a radiation-resistant bacterium isolated from laver was potentially identified as Micrococcus roseus sp.

• Journal of Pharmaceutical Investigation
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• 제36권5호
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• pp.309-314
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• 2006

The multicellular layers(MCL) of human cancer cells is a three dimensional(3D) in vitro model for human solid tumors which has been used primarily for the assessment of avascular penetration of anti-cancer drugs. For anti-cancer drugs with penetration problem, MCL represents a good experimental model that can provide clinically relevant data. Calcein-AM is a fluorescent dye that demonstrates the cellular vitality in a graded manner in cancer cell culture system. In the present study, we evaluated the use of calcein-AM for determination of anti-proliferative activity of anti-cancer agents in MCL model of DLD-1 human colorectal cancer cells. Optical sectioning of confocal imaging was compromised with photonic attenuation and penetration barrier in the deep layers of MCL. By contrast, fluorescent measurement on the cryo-sections provided a feasible alternative. Cold pre-incubation did not enhance the calcein-AM distribution to a significant degree in MCL of DLD-1 cells. However, the simultaneous determination of drug penetration and cellular vitality appeared to be possible in drug treated MCL. In conclusion, these data suggest that calcein-AM can be used for the simultaneous determination of drug-induced anti-proliferative effect and drug penetration in MCL model.

• 미생물학회지
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• 제31권3호
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• pp.179-183
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• 1993

A restricted facultatively methanol-oxidizing bacterium, Methylovorus sp. strain SS1, was isolate dfrom soil samples from Kuala Lumpur, Malaysia, through methanol-enrichment culture technique. The isolate was nonmotile Gram-negative rod and did not have complex internal membrane system. The colonies were small, pale-yellow, and raised convex with entire margin. The cell did not produce any spores and capsular materials. The cell was obligately aerobic and exhibited catalase, but no oxidase, activity. Plasmid, carotenoid pigment, and poly-.betha.-hydroxybutyric acid were not found. The guanine plus cytosine content of the DNA was 55%. The isolate was found to grow only on methanol methylamine, or glucose. Growth factors were not required. Cells growing on methanol was found to produce extracellular polysaccharides containing glucose, lactose, and fructose. Growth was optimal (t$_{d}$= 1.7) with 0.5%(v/v) methanol at 40.deg.C and pH 6.5. No Growth was observed at over 60.deg.C. Cell-free extracts of the methanol grown cells exhibited the phenazine methosulfate-linked methanol dehydrogenase activity Methanol was found to be assimilate dthrough the ribulose monophosphate pathway.y.

• 한국자원식물학회지
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• 제20권4호
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• pp.367-374
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• 2007

엽, 엽병과 화뢰 등의 치상 절편체 부위 모두에서 캘러스가 형성되었으며 특히 화뢰에서 캘러스 형성률이 71.6%로 가장 높았다. 화뢰배양시 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ TDZ의 혼용처리구에서 캘러스 형성이 77.8%로 양호했으며 신초 재분화율 역시 42.6%를 보였다. 캘러스 형성과 신초 재분화를 동시에 시킬 때 1/2 MS배지가 효과적이었는데 1/2 MS배지에서 캘러스 형성률은 46.3%, 신초 재분화율은 13.0%,그리고 발근율은 13.0%를 보였다. 캘러스를 통한 신초의 재분화를 위해 1/2 MS배지에 2.0mg ${\cdot}$ $L^{-1}$ 2,4-D와 1.0mg ${\cdot}$ $L^{-1}$ BA를 첨가했을 때 신초 재분화율은 74.1%를 보였고 절편체당 신초수는 2.4개로 좋았다. 발근율은 대조구에서 57.8%로 낮았으나 1.5mg ${\cdot}$ $L^{-1}$ NAA 첨가된 배지에서 97.8%로 높았다. 재분화된 기내 식물체를 원예용 상토에 순화했을 때 생존률이 86.1%로 높았으며 초장, 근장, 생체중 역시 양호하였다.

• 한국미생물·생명공학회지
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• 제38권3호
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• pp.335-339
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• 2010

본 연구에서는 디젤로 오염된 토양에서 디젤 분해능이 우수한 Gordonia sp. SD8을 분리하였고, 이 균주의 디젤 분해특성을 액상과 토양에서 조사하였다. SD8은 유일 에너지원과 탄소원으로 디젤을 이용하여 생장 가능하였다. SD8 균주의 성장과 디젤 분해속도에 미치는 디젤 농도 영향을 조사한 결과, 20,000 mg-TPH $L^{-1}$농도에서 최대 비성장속도($0.67{\pm}0.05\;d^{-1}$)와 최대분해속도($1,727{\pm}145$ mg-TPH $L^{-1}\;d^{-1}$)를 얻을 수 있었다. 또한, 이 균주는 40,000 mg-TPH $L^{-1}$의 고농도 디젤을 분해할 수 있었으며, $30^{\circ}C$에서 비성장속도와 디젤분해속도가 가장 빨랐다. 디젤로 오염된 토양 정화에 미치는 Gordonia sp. SD8 접종 효과를 조사한 결과, 17일 경과 후, SD8을 접종하지 않은 대조군 토양의 디젤 잔류 농도는 $8,150{\pm}755$ mg-TPH kg-dry $soil^{-1}$이었으나, SD8을 접종한 경우에는 3,724 mg-TPH kg-dry $soil^{-1}$이었다. 이러한 결과는 Gordonia sp. SD8는 향후 디젤 등을 포함한 석유계 탄화수소화합물로 오염된 토양을 정화하는데 활용 가능한 유용 미생물 자원임을 의미한다.

• KSBB Journal
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• 제20권4호
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• pp.278-284
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• 2005

식물세포의 느린 생장과 낮은 생산량의 이유로 지금까지 는 주로 미생물이나 동물세포에서 유전자 재조합 단백질을 생산하여 왔다. 그러나 저렴한 배지 가격, 동물 유래 바이러스 감염 위험성으로부터의 안정성, glycosylation 등의 post-translational modification이 가능하다는 장점들로 인하여 최근 들어 식물세포배양은 생물학적 활성을 가진 고부가가치의 단백질을 생산하는데 많이 이용되고 있다. 본 연구에서는 생장배지에 첨가했던 sucrose의 소비와 induction 배지로의 교환에서 오는 배지내의 삼투압을 조절하여 hCTLA4-Ig의 생산성을 높이고자 하였다. 다양한 삼투압 조절제 첨가 실험을 통해 sorbitol을 선별하고, 40 mM의 sorbitol 첨가에서 상대적으로 높은 생존도와 induction 후 7일째 대조구보다 1.7배 높은 생산성을 확인하였다. 또한, 저농도의 glucose 첨가를 통한 생산성 증대에 있어서는 8 mM glucose에서 induction 이후에도 높은 세포농도를 유지하면서 최대 37.3 mg/L까지 hCTLA4-Ig 생산량을 증가시켰다. 5-L bioreactor에서 회분식 배양과 induction시의 hCTLA4-Ig 생산량을 비교한 결과 induction시 배양 18일째 최고 45.3 mg/L까지 높일 수 있었으며, 회분식 배양에 비해 2.1배 증가됨을 확인하였다.

• 한국수정란이식학회지
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• 제32권3호
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• pp.87-94
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• 2017

Tissue engineering (TE) has been developed to create functional organs and tissue by combining 3D matrix and cells in vitro. Vascularization and angiogenesis are utmost important for supply of nutrients and oxygen in tissue engineered organs. The present study was performed to isolate and characterize primary endothelial cells (EC) from aorta of alpha 1, 3-enzyme galactosyltransferase knock out (GalT KO) pig, to minimize immune rejection and analyze body immune system for future xenotransplantation studies. Isolation of primary EC from aorta were performed by incubation with dispase for 8-10 min at $37^{\circ}C$. Primary EC were cultured in EC growth medium on different extra cellular matrix (ECM), either collagen or gelation. Primary EC exhibits morphological characteristics and showed positive expressions of EC specific marker proteins i.e. PECAM1, KDR and VWF despite of their ECM surface; however, on collagen based surface they showed increase in mRNA level analyzed by qPCR. Primary EC cultured on collagen were sorted by flow cytometer using KDR marker and cultured as KDR positive cells and KDR negative cells, respectively. KDR positive cells showed dramatically increased in PECAM1 and VWF level as compared to KDR negative cells. Based on the above results, primary EC derived from GalT KO are successfully isolated and survived continuously in culture without becoming overgrown by fibroblast. Therefore, they can be utilize for xeno organ transfer, tissue engineering, and immune rejection study in future.

• 한국수산과학회지
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• 제34권5호
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• pp.465-472
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• 2001

양식현장에서 선별작업시 처리되는 사육수조의 수심감소와 어류이송에 따른 넙치 (대, 소)와 큰민어의 스트레스 반응을 알아보기 위하여 스트레스 지표로 알려져 있는 혈액학적 요인, 코티졸, 글루코스, 젖산 및 삼투질 농도를 조사하였다. 수심감소에서 넙치대의 Ht는 실험전 $14.6\%$였던 것이 10시간째까지 계속 높아져 $23.5\%$를 보였으나, 실험종료시 (46시간째) $14.4\%$로 회복되었다. 넙치대의 코티졸 농도는 실험개시시에 1.9ng/mL였던 것이 22시간째에 13.7 ng/mL로 높아졌고, 46시간째에는 4.0ng/mL로서 개시시의 수준으로 회복되었다. 큰민어는 4시간째 282.3ng/mL, 22시간째 350.5ng/mL로 높아졌으나, 실험종료시에는 16.0ng/mL로 낮아졌다. 큰민어의 글루코스는 실험개시시 57.0mg/dL로부터 22시간째에는 138.0mg/dL조 유의하게 높아졌다 젖산은 모든 어종에서 유의차가 인정되지 않았다. 어류이송에 따른 넙치대의 RBC는 실험개시시의 $1.9\times10^6 cell/{\mu}L$로부터 실험종료시의 $4.2\times10^6 cell/{\mu}L$로 높아졌다. 큰민어의 Hb는 실험개시시의 9.8g/dL로부터 실험종료시에 20.4g/dL로 개시시 보다 유의하게 높았다. 넙치대의 코티졸 농도는 실험개시시의 1.2ng/mL로부터 이동 1시간째에 95.3ng/mL로 높아졌고, 큰민어도 실험개시시의 5.5ng/mL로부터 1시간째에 175.5ng/mL로 높아졌다. 큰민어의 글루코스는 1, 3시간째 각각 132.5 mg/dL, 129.5 mg/dL로 실험개시시 46.5 mg/dL 보다 높은 수준을 보였다.