• Title/Summary/Keyword: 3D Space

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Overexpression, Crystallization, and Preliminary X-Ray Crystallographic Analysis of the Alanine Racemase from Enterococcus faecalis v583

  • Priyadarshi, Amit;Lee, Eun-Hye;Sung, Min-Woo;Kim, Jae-Hee;Ku, Min-Je;Kim, Eunice Eun-Kyeong;Hwang, Kwang-Yeon
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.55-58
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    • 2008
  • Alanine racemase, a bacterial enzyme belonging to the fold-type III group of pyridoxal 5'-phosphate (PLP)-dependent enzymes, has been shown to catalyze the interconversion between L- and D-alanine. The alanine racemase from the pathogenic bacterium Enterococcus faecalis v583 has been overexpressed in E. coli and was shown to crystallize an enzyme at 295 K, using polyethylene glycol (PEG) 8000 as a precipitant. X-ray diffraction data to $2.5{\AA}$ has been collected using synchrotron radiation. The crystal is a member of the orthorhombic space group, $C222_1$ with unit cell parameter of a=94.634, b=156.516, $c=147.878{\AA},\;and\;{\alpha}={\beta}={\gamma}=90{\AA}$. Two or three monomers are likely to be present in the asymmetric unit, with a corresponding $V_m\; of\;3.38{\AA}^3\;Da^{-1}\;and\;2.26{\AA}^3\;Da^{-1}$ and a solvent content of 63.7% and 45.5%, respectively.

Spatial and Temporal Aspects of Phytoplankton Blooms in Complex Ecosystems Off the Korean Coast from Satellite Ocean Color Observations

  • Ahn, Yu-Hwan;Shanmugam, Palanisamy;Chang, Kyung-Il;Moon, Jeong-Eon;Ryu, Joo-Hyung
    • Ocean Science Journal
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    • v.40 no.2
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    • pp.67-78
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    • 2005
  • Complex physical, chemical and biological interactions off the Korean coast created several striking patterns in the phytoplankton blooms, which became conspicuous during the measurements of ocean color from space. This study concentrated on analyzing the spatial and temporal aspects of phytoplankton chlorophyll variability in these areas using an integrated dataset from a Sea-viewing Wide Field-of-view Sensor (SeaWiFS), Advanced Very High Resolution (AVHRR) sensor, and Conductivity Temperature Depth (CTD) sensor. The results showed that chlorophyll concentrations were elevated in coastal and open ocean regions, with strong summer and fall blooms, which appeared to spread out in most of the enclosed bays and neighboring waters due to certain oceanographic processes. The chlorophyll concentration was observed to range between 3 and $54\;mg\;m^{-3}$ inside Jin-hae Bay and adjacent coastal bays and 0.5 and $8\;mg\;m^{-3}$ in the southeast sea offshore waters, this gradual decrease towards oceanic waters suggested physical transports of phytoplankton blooms from the shallow shelves to slope waters through the influence of the Tsushima Warm Current (TWC) along the Tsushima Strait. Horizontal distribution of potential temperature $(\theta)$ and salinity (S) of water off the southeastern coast exhibited cold and low saline surface water $(\theta and warm and high saline subsurface water $({\theta}>12^{\circ}C; S>34.4)$ at 75dBar, corroborating TWC intrusion along the Tsushima Strait. An eastward branch of this current was called the East Korean Warm Current (EKWC), tracked with the help of CTD data and satellite-derived sea surface temperature, which often influenced the dynamics of mesoscale anticyclonic eddy fields off the Korean east coast during the summer season. The process of such mesoscale anticyclonic eddy features might have produced interior upwelling that could have shoaled and steepened the nutricline, enhancing phytoplankton population by advection or diffusion of nutrients in the vicinity of Ulleungdo in the East Sea.

Gesture based Input Device: An All Inertial Approach

  • Chang Wook;Bang Won-Chul;Choi Eun-Seok;Yang Jing;Cho Sung-Jung;Cho Joon-Kee;Oh Jong-Koo;Kim Dong-Yoon
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.5 no.3
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    • pp.230-245
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    • 2005
  • In this paper, we develop a gesture-based input device equipped with accelerometers and gyroscopes. The sensors measure the inertial measurements, i.e., accelerations and angular velocities produced by the movement of the system when a user is inputting gestures on a plane surface or in a 3D space. The gyroscope measurements are integrated to give orientation of the device and consequently used to compensate the accelerations. The compensated accelerations are doubly integrated to yield the position of the device. With this approach, a user's gesture input trajectories can be recovered without any external sensors. Three versions of motion tracking algorithms are provided to cope with wide spectrum of applications. Then, a Bayesian network based recognition system processes the recovered trajectories to identify the gesture class. Experimental results convincingly show the feasibility and effectiveness of the proposed gesture input device. In order to show practical use of the proposed input method, we implemented a prototype system, which is a gesture-based remote controller (Magic Wand).

Building frame - pile foundation - soil interaction analysis: a parametric study

  • Chore, H.S.;Ingle, R.K.;Sawant, V.A.
    • Interaction and multiscale mechanics
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    • v.3 no.1
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    • pp.55-79
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    • 2010
  • The effect of soil-structure interaction on a single-storey, two-bay space frame resting on a pile group embedded in the cohesive soil (clay) with flexible cap is examined in this paper. For this purpose, a more rational approach is resorted to using the finite element analysis with realistic assumptions. Initially, a 3-D FEA is carried out independently for the frame on the premise of fixed column bases in which members of the superstructure are discretized using the 20-node isoparametric continuum elements. Later, a model is worked out separately for the pile foundation, by using the beam elements, plate elements and spring elements to model the pile, pile cap and soil, respectively. The stiffness obtained for the foundation is used in the interaction analysis of the frame to quantify the effect of soil-structure interaction on the response of the superstructure. In the parametric study using the substructure approach (uncoupled analysis), the effects of pile spacing, pile configuration, and pile diameter of the pile group on the response of superstructure are evaluated. The responses of the superstructure considered include the displacement at top of the frame and moments in the columns. The effect of soil-structure interaction is found to be quite significant for the type of foundation considered in the study. Fair agreement is observed between the results obtained herein using the simplified models for the pile foundation and those existing in the literature based on a complete three dimensional analysis of the building frame - pile foundation - soil system.

Design for Radiotherapy Room with High Density Shielding Block (고 강도 차폐벽돌을 이용한 방사선치료실의 차폐설계)

  • Suh Chang Ok;Kim Gwi Eon;Chu Sung Sil
    • Progress in Medical Physics
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    • v.15 no.4
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    • pp.247-254
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    • 2004
  • According to developing high energy linear accelerators and treatment methods, like (3 dimensional conformal radiotherapy (3D-CRT), intensity modulated radiotherapy (IMRT), many radiotherapy centers are replacing older linear accelerators with new higher technical machines. This often presents a shielding problem as the designed shield for the existing rooms is not adequate for the higher technical machines. Additional shielding in limited existing space becomes necessary. We are replacing older brachytherapy room with new higher technical linear accelerator for IMRT. This room is not adequate for the IMRT machine without additional shielding design. The logical development of optimum structural shielding designs with concrete and high density shielding blocks are presented. We obtained following results by comparison between the pre-calculating values and actual survey of completed LINAC installation. High density shielding blocks have more powerful radiation protection about 2 times.

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A Hybrid Shadow Testing Scheme During Ray Tracing (광선추적 수행중 혼합 음영검사에 관한 연구)

  • Eo, Kil-Su;Kyung, Chong-Min
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.26 no.3
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    • pp.95-104
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    • 1989
  • This paper presents a new shadow testing acceleration scheme for ray tracing called Hybrid Shadow Testing (HST) based on a conditional switching between the conventinal shadow testing method and Crow's shadow volume method, where the shadow polygons as well as the object polygons are registered onto the corresponding cells under the 3-D space subdivision environment. Despite the preprocessing time for the generation and registration of the shadow polygons, the total shadow testing time of the proposed algorithm, HST was approximately 50% of that of the conventional shadow testing method for several examples while the total ray tracing time was typically reduced by 30% from the conventional approach. This due to the selective use of the shadow volume method with a compromise between the maximal utilisation of shadow's spatial coherency and minimising the computational overhead for checking ray intersections with the shadow polygons. A parameter, $N_{th}$ denoting the critical number of shadow polygons between successive reflection points was used as a guideline for switching the shadow testing scheme between the conventional method and shadow volume method. A method for calculating $N_{th}$ from such statistical data as the number of object polygons, average polygon size average peripheral length of the polygons was proposed, resulting in good agreement with the experimental results.

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The economic benefit of combustible waste into energy: A contingent valuation study (Fe3C12 식각을 이용한 콘덴싱 보일러 잠열 열교환기의 응축 열전달 촉진)

  • Jang, J.H.;Ahn, J.;Shin, D.H.;Chung, T.Y.
    • Journal of Energy Engineering
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    • v.22 no.3
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    • pp.307-311
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    • 2013
  • Heating and hot water has accounted for 68% of Korea's household energy usage. Boiler makes up the bulk of the heating and hot water production. Hence a highly efficient boiler is needed in order to reduce energy consumption. A condensing boiler that recovers latent heat is known to be highly efficient. However, it is expensive and takes more space to necessitate research for improvement. In the present study, we investigated condensation heat transfer of a surface roughened by etching treatment. The etched plate showed 9.2% increase in heat transfer compared to original plate.

A Landmark Based Localization System using a Kinect Sensor (키넥트 센서를 이용한 인공표식 기반의 위치결정 시스템)

  • Park, Kwiwoo;Chae, JeongGeun;Moon, Sang-Ho;Park, Chansik
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.63 no.1
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    • pp.99-107
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    • 2014
  • In this paper, a landmark based localization system using a Kinect sensor is proposed and evaluated with the implemented system for precise and autonomous navigation of low cost robots. The proposed localization method finds the positions of landmark on the image plane and the depth value using color and depth images. The coordinates transforms are defined using the depth value. Using coordinate transformation, the position in the image plane is transformed to the position in the body frame. The ranges between the landmarks and the Kinect sensor are the norm of the landmark positions in body frame. The Kinect sensor position is computed using the tri-lateral whose inputs are the ranges and the known landmark positions. In addition, a new matching method using the pin hole model is proposed to reduce the mismatch between depth and color images. Furthermore, a height error compensation method using the relationship between the body frame and real world coordinates is proposed to reduce the effect of wrong leveling. The error analysis are also given to find out the effect of focal length, principal point and depth value to the range. The experiments using 2D bar code with the implemented system show that the position with less than 3cm error is obtained in enclosed space($3,500mm{\times}3,000mm{\times}2,500mm$).

A Kinetics Analysis of Forward 11/2 Somersault on the Platform Diving (플랫폼 다이빙 앞으로 서서 앞으로 11/2회전 동작의 운동역학적 분석)

  • Jeon, Kyoung-Kyu
    • Korean Journal of Applied Biomechanics
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    • v.23 no.3
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    • pp.209-218
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    • 2013
  • This study was to perform the kinetic analysis of forward $1\frac{1}{2}$ somersault on the platform diving. Six men's diving players of the Korea national reserve athletes participated in this study. The variables were analyzed response time, velocity, center of mass (COM), angle, center of pressure (COP) and ground reaction force (GRF) of motion. For measure and analysis of this study, used to synchronized to 4 camcorder and 1 force plate, used to the Kwon3D XP (Ver. 4.0, Visol, Korea) and Kwon GRF (Ver. 2.0, Visol, Korea) for analyzed of variables. The results were as follows; Time factor were observed in maximum knee flexion depending on the extent of use at phase 1 of take-off to execute the somersault. This enabled the subject to secure the highest possible body position in space at the moment of jumping to execute the somersault and prepare for the entry into the water with more ease. Regarding the displacement of COM, all subjects showed rightward movement in the lateral displacement during technical execution. Changes in forward and downward movements were observed in the horizontal and vertical displacements, respectively. In terms of angular shift, the shoulder joint angle tended to decrease on average, and the elbow joints showed gradually increasing angles. This finding can be explained by the shift of the coordinate points of body segments around the rotational axis in order to execute the half-bending movement that can be implemented by pulling the lower limb segments toward the trunk using the upper limb segments. The hip joint angles gradually decreased; this accelerated the rotational movement by narrowing the distance to the trunk. Movement-specific shifts in the COP occurred in the front of and vertical directions. Regarding the changes in GRF, which is influenced by the strong compressive load exerted by the supporting feet, efficient aerial movements were executed through a vertical jump, with no energy lost to the lateral GRF.

Application of Full-Face Round by Sequential Blasting Machine in Tunnel Excavation (터널굴착에서 다단식 발파기에 의한 전단면 발파의 적용성 연구)

  • 조영동;이상은;임한욱
    • Tunnel and Underground Space
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    • v.4 no.2
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    • pp.132-143
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    • 1994
  • Many methods and techniques to reduce ground vibrations are well known. Some of them are to adopt electric millisecond detonators with a sequential blasting machine or an initiating system with an adequate number of delay intervals. The types of electric detonators manufactured in korea include instantaneous, decisecond and millisecond delays but numbers of delay intervals are only limited from No.1 to No.20 respectively. It is not sufficient to control accurately millisecond time with these detonators in tunnel excavation. Sequential fire time refers to adding an external time delay to a detonators norminal firing time to obtain sequential initiation and it is determined by sequential timer setting. To reduce the vibration level, sequential blasting machine(S.B.M) with decisecond detonators was adopted. A total of 134 blasts was recorede at various sites. Blast-to-structure distances ranged from 20.3 to 42.0 meter, where charge weight varied from 0.24 to 0.75 kg per delay. The results can be summarized as follow: 1. The effects of sequential blasting machine on the vibration level are discussed. The vibration level by S.B.M are decreased approximately 14.38~18.05% compare to level of conventional blasting and cycle time per round can be saved. 2. The empirical equations of particle velocity were obtained in S.B.M and conventional blasting. V=K(D/W1/3)-n, where the values for n and k are estimated to be 1.665 to 1.710 and 93.59 to 137 respectively. 3. The growth of cracks due to vibrations are found but the level fall to within allowable value.

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