• Title/Summary/Keyword: 3BC

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Increased Expression of the ${\alpha}_2$ Isoform of (Na,K)ATPase in the Differentiated Murine Muscle Cell Line BC3H-1 (BC3H-1 분화세포에서의 (Na,K)ATPase ${\alpha}_2$ isoform의 표현증대)

  • Lee, Kyung-Lim
    • YAKHAK HOEJI
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    • v.40 no.6
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    • pp.734-738
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    • 1996
  • The development of the alpha2 isoform of (Na,K)ATPase which is high affinity ouabain receptors was studied in the differentiating nonfusing muscle cell line BC3H-1. T he differentiation process of BC3H-1 cell line was confirmed by 2-dexy-D-[$^3$H] glucose uptake experiment and the quantity of the expression of ${\alpha}_2$ isoform was measured using a whole cell [$^3$H] ouabain-binding assay. Undifferentiated growing BC3H-1 cells, myoblasts, exhibited low levels of insulin-stimulated glucose uptake and [$^3$H] ouabain-binding sites. In contrast, differentiated BC3H-1 cells, myocytes, had a 5.6-fold increase in insulin-stimulated glucose uptake and 5-fold increase in [$^3$H] ouabain-binding sites. Scatchard analysis showed that myocytes developed more [$^3$H] ouabain-binding sites than myoblasts vath a dissociation constant (kd) of 6${\times}10^{-8}$M and capacity of 6.l${\times}10^{-5}$ sites/cell. Therefore. it seems that myoblasts express low levels of ${\alpha}_2$ subunit and probably the majority of ${\alpha}_1$ subunit, whereas myocytes express high levels of ${\alpha}_2$ isoform. The results indicate that the expression of ${\alpha}_2$ isoform is developmentally regulated during differentiation and that BC3H-1 culture system provides an excellent model for the study of differentiation and mechanism of (Na,K)ATPase action in muscle which requires electrical excitability.

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Performance Evaluation of Overlaid Single Frequency Broadcast and Cellular network using Superposition Coding (중첩부호를 이용한 단일주파수 방송 서비스 및 데이터 서비스 오버레이 시스템의 성능 평가)

  • Yoon, Seok-Hyun;Kim, Dong-Hee
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.46 no.7
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    • pp.32-38
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    • 2009
  • In this paper, we consider the overlaid system of single frequency network broadcast service and cellular-based unicast data service. In general, broadcast service and unicast data service were considered as a separate services so that different frequency resources have been allocated for each. As the frequency resource get more expensive, however, there were some efforts to provide both the broadcast and unicast service over the same frequency resource by employing the so-called superposition coding. In fact, such an service overlay system has already been accepted as a standards in 3GPP long-term evolution (LTE). In this paper, we specifically investigate such overlay system within 3GPP framework and evaluate their performance in terms of BC coverage and UC data throughput.

부패된 사과로부터 분리된 미생물의 bacterial cellulose 생산특성 (Production of bacterial cellulose by a mircobial strain isolated from rotten apples)

  • Jeong, Jae-Yong;Park, Yeon-Hui;Park, Jung-Gon
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.465-468
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    • 2002
  • 부패한 사과로부터 bacterial cellulose (BC)를 생산할 수 있는 균주를 분리한 후 배양조건에 따른 BC의 생산량을 조사한 결과 BC의 생산량은 진탕배양한 경우가 정치배양한 경우보다 약 1.5배 높았다. BC의 생산량을 높이고자 mutagen으로 UV와 cylcloheximide를 사용함으로써 BC 생산량을 약 3배 증가시킬 수 있었다.. 미생물에 의해 생성된 BC는 종이나 펄프와는 달리 pectin, 납, 유지, 단백질, 무기질 등의 불순물을 함유하지 않는 filter paper와 성질이 유사한 것으로 나타났다.

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BcN 인프라 정보보호

  • 전용희;장종수
    • Review of KIISC
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    • v.15 no.3
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    • pp.13-28
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    • 2005
  • 광대역 통합망(BcN: Broadband convergence Network)에서 대역폭의 증가와 서비스의 통합으로 보안의 중요성이 빠르게 증가하고 있다. 그러므로 보안은 BcN에서 다루어져야 할 중요한 문제의 하나로 인식되어야 한다. BcN과 같은 QoS-aware 통신시스템에서 사용자는 원하는 서비스 품질 수준의 보장을 위하여 여러 가지의 서비스 클래스 중에서 선택할 수 있다. 그러나 보안은 아직 이런 QoS 구조에서 하나의 파라미터로 인정되지 않고 있으며, 보안-관련 서비스 클래스도 정의된 바 없다. 본고에서는 BcN 구조에서 발생할 수 있는 보안 취약성을 분석하고 BcN 인프라 정보보호를 위하여 관련 기술, 표준화, 국내 동향 등에 대하여 기술하고자 한다.

광대역 통합망(BcN) 시범 서비스 추진동향

  • 신용식;박용길;정원석;임종태
    • Information and Communications Magazine
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    • v.21 no.11
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    • pp.92-103
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    • 2004
  • 광대역 통합망은 유무선 통합, 통신과 방송 응합, 음성 및 데이터의 통합을 위해 필요한 네트워크를 서비스 계층, 제어 계층, 전달망 계층, 접속 계층, 유비쿼터스 접속 및 가입자 단말 계층으로 구분한다. BcN은 사용자가 원하는 품질과 보안의 정도에 따라 차별화된 광대역 멀티미디어 서비스 제공 및 관리가 가능한 네트워크이다. 본고는 현재 진행 중인 BcN 시범 사업에 대하여 기술한다. 우선, 통합 서비스를 제공하기 위한 기본 인프라인 광대역 통합망(BcN, Broadband convergence Network)의 개념, 서비스 특징 및 발전방향 등을 간략히 나타낸다. 그리고 정부의 IT839 전략의 중점 추진과제로 진행 중인 1단계 BcN 시범 사업의 내용과 시범 사업자로 선정된 3개 컨소시엄의 서비스 내용 등을 제시한다. 현재 BcN 시범 사업자로 UbiNet 컨소시엄, Octave 컨소시엄 및 광개토 컨소시엄이 선정된 상태이다. 각 컨소시엄에서는 BcN 환경에 적합한 다양한 서비스 모델 발굴 및 기술개발을 위해 BcN 시범 서비스를 진행할 예정이며, 제공 예정인 BcN 시범 서비스에는 음성 데이터 통합 서비스, 유무선 통합 서비스, 통방 융합 서비스 및 기타 응용 서비스 등이 있다.

Degradation of MS(Linear Alkylbenzene Sulfonate) by Plasmid (LAS(Linear Alkybenzene Sulfonate)의 Plasmid에 의한 분해)

  • 차전옥;유진삼;백형석
    • Microbiology and Biotechnology Letters
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    • v.22 no.2
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    • pp.158-163
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    • 1994
  • Microorganisms capale of utilizing linear alkylbenzene sulfonates(LAS) as sole carbon source were isolated from industrial effluent by using LAS agar plates. The isolated strains were identified as Salmonella sp(BC-2) and Escherichia sp.(BC-3) from the results of morphological, cultural and biochemical tests. The optimal condition for the growth and biodegradation of LAS was the initial pH 7.0 and LAS concentration 0.1%. The isolated BC-2 and BC-3 strains harbored plasmid and LAS-degrading activity was lost when the plasmids were cured by mitomycin C. The plasmids were transformed into E. coli and transformants have the LAS-degrading activity. Isolated strains were examined for primary biodegradation rate of LAS in the medium by methylene blueactive substance(MBAS) method. Of these isolates, BC-2 and BC-3 strains degradated LAS upto 60% and high resistant to CdCl$_{2}$ and HgCl$_{2}$. Isolated strains were sensitive to chloramphenicol, kanamycin, rifampicin, streptomycin and tetracycline but resistant to ampicillin and lincomycin.] Its minimal inhibitory concentration(MIC) for ampicillin was more than 1500 $\mu $g/ml.

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Occupational Factors Influencing the Forklift Operators' Exposure to Black Carbon (지게차 운전원의 블랙카본(black carbon, BC) 노출에 영향을 미치는 직업적 요인)

  • Lee, Hyemin;Lee, Seunghee;Ryu, Seung-Hun;Park, Jihoon;Park, Dong-Uk
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.27 no.4
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    • pp.313-323
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    • 2017
  • Objectives: This study aimed to assess exposure to black carbon(BC) among forklift operators and to identify environmental and occupational factors influencing their BC exposure. Methods: We studied a total of 23 forklift operators from six workplaces manufacturing paper boxes. A daily BC exposure assessment was conducted during working hours from January to April 2017. A micro-aethalometer was used to monitor daily BC exposure, and information on work activities was also obtained through a time-activity diary(TAD) and interviews. BC exposure records were classified into four categories influencing BC exposure level: working environment, workplace, forklift operation, and job characteristics. Analysis of variance(ANOVA) was used to compare average BC exposure levels among the four categories and the relationships between potential factors and BC exposure were analyzed using a multiple linear regression model. Results: The operators' daily exposure was $12.9{\mu}g/m^3$(N=9,148, $GM=7.5{\mu}g/m^3$) with a range: $0.001-811.4{\mu}g/m^3$. The operators were exposed to significantly higher levels when they operate a forklift in a room ${\leq}20,000m^3$($AM=12.3{\mu}g/m^3$), in indoor workplaces($AM=16.3{\mu}g/m^3$), when they operate a forklift manufactured before 2006 ($AM=13.2{\mu}g/m^3$), a forklift with a loading limit of four-tons($AM=27.1{\mu}g/m^3$), with a roll and bale type clamp($AM=17.1{\mu}g/m^3$), and with no particulate filter($AM=15.7{\mu}g/m^3$). Conclusions: Occupational factors including temperature, smoking, season, daytime, room volume($m^3$), location of operating, and manufacturing era and model of forklift influenced the BC exposure of forklift operators. The results of this study can be used to minimize the BC exposure of forklift operators.

Cloning of Thermophilic Alkalophilic Bacillas sp. F204 Cellulase Gene and Its Expression in Escherichia coli and Bacillus subtilis (고온 알칼리성 Bacillus sp. F204의 Cellulase 유전자의 Escherichia coli 및 Bacillus subtilis에의 Cloning 및 발현)

  • Chung, Young-Chul;Kim, Yang-Woo;Kang, Shin-Kwon;Rho, Jong-Su;Park, Jae-Hyeon;Sung, Nack-Kie
    • Korean Journal of Food Science and Technology
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    • v.23 no.1
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    • pp.31-36
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    • 1991
  • Cellulase genes from thermophilic alkalophilic Bacillus sp. F204 a potent cellulase complex-producing bacterium, were cloned in Escherichia coli with pUC 19. Plasmids pBC191 and pBC192, isolated from transformants forming yellow zone around colony on the LB agar plate containing 0.5% carboxymethyl cellulose and ampicillin, contained 4.6 Kb and 5.8 Kb HindIII fragments, respectively. The 4.6 Kb insert of pBC191 had single sites for BamHI EcoRI, KpnI and pvuII. DNA hybridization and immunodiffusion studies showed that pBC191-encoded cellulase gene was homologous with that of host strain. pKC231, constructed by inserting 4.6 Kb insert of pBC191 at the HindIII site of pKK223-3, E. coli expression vector, and pGC711, constructed by inserting 4.6 Kb insert of pBC191 at the HindIII site of pGR71, E. coli and B. subtilis shuttle vector, had 3.2 times and 2.8 times as much cellulase activity as pBC191, respectively. Substrate specificity analysis showed that cellulases cloned were CMCase.

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Vegetation History since the Mid-Lateglacial from Yeongsan River Basin, Southwestern Korea (영산강 유역 범람원 퇴적물의 화분분석 연구)

  • Choi, Kee-Ryong;Kim, Ki-Heon;Kim, Jong-Won;Kim, Jong-Chan;Lee, Gi-Kil;Yang, Dong-Yoon;Nahm, Wook-Hyun
    • The Korean Journal of Ecology
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    • v.28 no.1
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    • pp.37-43
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    • 2005
  • This study presents the Mid-Lateglacial vegetation history of southwestern Korea. From the result of AMS dating and pollen analysis, four local pollen assemblage zones(BS) were recognized. The four local pollen assemblage zones are BS 1. 12,222cal BC$\sim$9,160cal BC: cool-temperate northern/altimontane mixed coniferous & deciduous broad-leaved forest, BS 2. 9,160cal BC$\sim$ 4,210cal BC: cool-temperate central/montane deciduous broad-leaved forest, BS 3. 4,210cal BC$\sim$125cal BC: cool-Temperate southern/submontane mixed coniferous & deciduous broad-leaved forest. BS 4. BC125cal ${\sim}$present: warm-temperate/cool-temperate southern/submontane coniferous forest. Pine forest expanded since 4,210cal BC and full-scale rice cultivation might started since 2,120cal BC. A radical expansion of Pinus densiflora forest after postglacial stage might be caused by human impacts including full-scale agriculture.

The Study of Voice Traffic Comparison From PSTN with the BcN after converting (PSTN에서 BcN으로 전환 후 음성 트래픽 비교에 관한 연구)

  • Lee, Young-Hag;Kim, Seok;Han, Seung-Jo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2007.10a
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    • pp.600-603
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    • 2007
  • 미래 지식정보사회는 컴퓨터, 통신, 방송 등 모든 정보통신 기기가 하나의 네트워크에 연결되는 BcN 기반의 네트워크사회(Broadband Network Society)로 빠르게 진화할 전망이다. BeN은 엑세스망, IP전달망, 제어/서비스망(교환망 IP전환)의 3개 계층을 모두 포함하는 개념이나 엑세스망, IP전달망은 기존과 통일한 IP방식으로 추진 중이어서 IP기반의 제어/서비스망 신규 구축 및 교환망의 IP로의 전환을 협의의 BcN이라 정의 한다. PSTN에서 BcN망으로 전환 시 BeN망의 구성 요소는 소프트스위치, TGW, AGW, SGW, 응용서버, 미디어서버 등이 있다. PSTN망이 BcN망으로 전환시 크게 3단계의 과정을 거친다. 1단계로 2007년 상반기에 전남 목포지역의 지능망 서비스가 불가한 시외교환기가 BcN으로 전환되었다. 전환 후의 IP망을 경유하는 음성호의 트래픽을 측정한 결과는 발생호는 증가하였으며 완료율은0.06% 감소하였지만 관리 기준내의 양호한 수준이었다. 향후 음성호의 통화 품질을 높이기 위해서 품질 측정을 통한 기준 미달 항목을 조치하고 트래픽 측정을 통한 소통율, 완료율 불량 구간에 대한 지속적인 개선 활동을 해야 한다.

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