• Title/Summary/Keyword: 360-degree oblique illumination

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Dark Field Digital Holographic Microscopy Based on Two-lens 360-degree Oblique Illumination

  • Zhang, Xiuying;Zhao, Yingchun;Yuan, Caojin;Feng, Shaotong;Wang, Lin
    • Current Optics and Photonics
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    • v.4 no.3
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    • pp.193-199
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    • 2020
  • In this paper we propose a dark-field digital holographic microscopy system based on 360-degree oblique illumination. This setup is constructed without using a dark-field objective. The principle of 360-degree oblique illumination of vortex beam and dark-field digital holographic microscopy are introduced theoretically and experimentally. By analyzing the reconstructed image of a dark-field digital hologram of a USAF 1951 target, it is proved that the imaging resolution can be improved by this method. And also, comparison and analysis are made on the reconstructed image of a bright-dark field digital hologram of a pumpkin stem slice, the result shows that the imaging contrast is also enhanced with this method, and it is effective for dark-field digital holographic microscopy imaging of large transparent biological samples.