No, Hoon-Jeong;Moon, Gu;Moon, Seok-Jae;Won, Jin-Hee;Moon, Young-Ho;Park, Rae-Gil
THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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v.6
no.1
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pp.81-97
/
2000
Objectives: This experimental study was carried out to evaluate the effects of aqueous and methanol extracts of Hedyotis diffusa which has long been used for cancer treatment in oriental medicines on the induction of apoptotic cell death in human lymphoid leukemia cell line, HL-60. Methods: Cells were treated with various concentrations (200 to $0.4{\mu}g$) and periods (6 to 30 hr) of $H_2O$ and methanol extracts of Hedyotis diffusa. Then, cells were tested for viability by MTT assay. Cells wrere treated with $200{\mu}g/ml$ of methanol extract fork various periods. Genomic DNA was isolated, separated, on 1.5% agarose gels, stained with ethidium bromide and visualized under UV light. Cells were treated with $200{\mu}g/ml$ of each extract for 16 hr. Then, cells were treated with Hoechst dye 33342 and observed by fluorescence microscopy. Cells were treated with various doses of each for 12 hr and $100{\mu}g/ml$ of methanol extract for various periods. Lysate from the cells used to measure the activity of Caspase-1 and-3 proteases by using fluorogenic peptide substrates including acetyl-YVAD-AMC and acetyl-DEVD-AMC, respectively. Cells were treated with $200{\mu}g/ml$ of each extract for various periods. Cell lysates were immunoprecipated with anti-JNKl antibodies. The immune complex was reacted with $32^p-ATP$ and c-Jun as a substrate. The phosphotransferase activity of JNKI was measured by using PhosphoImage analyzer (Fuji Co., Japan). Nuclear extracts were isolated and incubated with oligonucleotide probe of $NF-{\kappa}B$. Transcriptional activation of ${\kappa}B$ was measured by using EMSA and visualized by PhosphoImage analyzer (Fuji Co, Japan). Cell lysates were prepared and analyzed by Western blotting with anti-Bc12 antibodies and anti-Bax antibodies. Cells were pretreated with various doses of methanol extract for 2 hr. Then, the extract was removed by centrifugation. Cells were resuspended with RPMI-1640 media containing 0.3% agarose, 10% FBS, overlayred onto bottom layer agarose and incubated at $CO_2$ incubator for 6 days. The number of colony was counted under light microscopy ($\time100$). Results: The death of HL-60 cells was markedly induced by the addition of methanol extract of Hedyotis diffusa in a dose and time-dependent manners. The apoptotic characteristic ladder pattern of DNA strand break was observed in death of HL-60 cells. In addition, it was shown nucleus chromatin condensation and fragmentation under Hoechst staining. Therefore, Hedyotis diffusa extract-induced death of HL-60 cells is mediated by apoptotic signaling processes. The activity of Caspase 3-like proteases remained in a basal level in HL-60 cells treated with aqueous extract of Hedyotis diffusa. However, it was markedly increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. In addition, the phosphotransferase activity of JNKl was increased in HL-60 cells treated with methanol extract of Hedyotis diffusa. Furthermore, the activation of transcriptional activator, $NF-{\kappa}B$ was markedly induced by methanol extract of Hedyotis diffusa. Anti-apoptotic Bc12 was cleaved into 23Kda fragment by treatment of methanol extract of Hedyotis diffusa. However, expression of proapoptotic Bax protein was increased by treatment of methanol extract of Hedyotis diffusa in a time-dependent manner. Furthermore, methanol extract markedly inhibited the colony forming efficiency of HL-60 cells in semisolid agar culture. Conclusions: Above results suggest that methanol extract of Hedyotis diffusa induces the apoptotic death of human leukemic HL-60 cells via activations of Caspase-3 proteases, JNKI, transcriptional activator $NF-{\kappa}B$, In addition, our results also suggest that methanol extract of Hedyotis diffusa reduces the malignant potential of HL-60 cells via down regulation of colony forming effciency through cleavage of Bc12 as well as induction of Bax.
In order to investigate the utilization of the Omija (Schizandra chinensis Baillon) extract as a natural coagulant for manufacturing soybean curd, the quality characteristics of white (Baktae) and black (Seoritae) soybean curds, coagulated by the Omija extract or $MgCl_2$, were evaluated. Crude protein ($6.14{\pm}0.30$ and $6.25{\pm}0.18%$, respectively) and crude lipid ($10.86{\pm}1.74$ and $11.29{\pm}1.69%$, respectively) contents of white and black soybean curds coagulated using the Omija extract were higher than those coagulated using $MgCl_2$. Black soybean curds coagulated using the Omija extract showed higher L, a, and b values than those using $MgCl_2$. The most abundant amino acid in white and black soybean curds coagulated using the Omija extract was arginine (3.74 and 3.71 mg/100 g, dry basis, respectively). The amounts of Ca, K, Mg, and Na were the highest in both soybean curds prepared with the Omija extract. The sensory evaluation (color, flavor, taste, texture, and overall preference) showed that white and black soybean curds coagulated using the Omija extract were more preferred than those produced using $MgCl_2$. The results suggested that using the Omija extract as a natural coagulant agent could improve the quality and sensory characteristics of soybean curds.
Oh, Sei-Chang;Ahn, Sye-Hee;Choi, In-Gyu;Jeong, Han-Seob;Yoon, Young-Ho;Yang, In
Journal of the Korean Wood Science and Technology
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v.36
no.3
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pp.30-38
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2008
Petroleum-based resin adhesives have extensively been used for the production of wood panels. However, with the increase of manufacturing cost and the environmental issue, such as the emission of volatile organic compounds, of the adhesive resins, it is necessary to be developed new adhesive systems. In this study, the potential of okara, which is a residue wasted from the production of tofu, for the development of bio-based adhesives was investigated. At first, the physical and chemical properties of okara were examined. After okara was hydrolyzed in acidic and/or alkaline solutions, okara-based adhesive resins were formulated with the mixtures of the okara hydrolyzates and phenol formaldehyde (PF) prepolymer. The adhesive resins were used for the fabrication of plywood panels, and then the adhesive strength and formaldehyde emission of the plywood panels were measured to examine the applicability of the resin adhesives for the production of plywood panels. The solids content and pH of the okara used in this study were around 20% and weak acidic state, respectively. In the analysis of its chemical composition, the content of carbohydrate was the highest, and followed by protein. The shear strengths of plywood fabricated with okara-based resin adhesives exceeded a minimum requirement of KS standard for ordinary plywood, but its wood failure did not reach the minimum requirement. In addition, the formaldehyde emissions of all plywood panels were higher than that of E1 specified in the KS standard. Based on these results, okara has the potential to be used as a raw material of environmentally friendly adhesive resin systems for the production of wood panels, but further researches - biological hydrolysis of okara and various formulations of PF prepolymer - are required to improve the adhesive strength and formaldehyde emission of okara-based resin adhesives.
Park, Kee-Jai;Lee, Ho-Joon;Kim, Jong-Hoon;Kwon, Ki-Hyun;Jeong, Jin-Woong
Korean Journal of Food Science and Technology
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v.38
no.6
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pp.742-750
/
2006
This study was carried out to investigate the efficacy of two kinds of electrolyzed water with added 0.5% (v/v) citron juice, SAEW-CJ[Strong Acidic Electrolyzed Water with added Citron Juice, pH 2.57, ORP (oxidation-reduction potential) 1,122 mV, HClO 23.05ppm] and LAEW-CJ (Low Alkaline Electrolyzed Water with added Citron Juice, pH 4.67, ORP 997mV, HClO 42.55mV) as storing liquid for peeled taro. During storage at $5^{\circ}C$ until 30 days, SAEW-CJ and LAEW-CJ inhibited the growth of microorganisms more effectively than 0.2% (w/v) APS (aluminium potassium sulfate) and 0.85% (w/v) NaCl did. Total phenolic contents, PRO (polyphenol oxidase) activity, color differences value (${\Delta}E$) and vitamin C contents of peeled taro stored in SAEW-CJ and LAEW-CJ were lower than those stored in 0.2% APS and 0.85% NaCl. The hardness decrement of peeled taro stored in LAEW-CJ was lower than that of the others. In addition, the contents of moisture, crude protein, crude ash, total sugars, and reducing sugars were gradually decreased during storage. However, no difference by peeling methods or immersion liquid was found.
LEE Keon-Young;PARK Sang-Min;AN Hee-Woo;CHO Hyun-Duk;HAN Bong-Ho
Korean Journal of Fisheries and Aquatic Sciences
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v.26
no.1
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pp.26-30
/
1993
To suggest a thermal diffusivity predicting equation for mixed meat products, heat penetration curves of pork products containing filefish meat were plotted in the temperature range of $80.44{\sim}121.03^{\circ}C$, and thermal diffusivities were calculated from the heat penetration curves. The ground pork was mixed with minced filefish meat and some additives such as lard, isolated soy protein, $1.5\%$ of table salt and $2\%$ of polyphosphate to control the composition and texture of products, and then stuffed into a model can. The heat penetration curves were plotted using a thermocouple fixed at the slowest heating point of the can. At constant heating temperature, the thermal diffusivities of the products increased linearly with increasing moisture content. The values of the products with constant moisture content also increased linearly with increasing heating temperature. The thermal diffusivities of the products with moisture content of $51.47{\sim}80.20\%$ could be predicted by following equation: $${\alpha}_p=(3.045+0.59{\cdot}X_w){\cdot}{\alpha}_w+0.0098{\cdot}10^{-6}{\cdot}X_w-0.4287{\cdot}10^{-6},(m^2{\cdot}s^{-1})$$ Maximal differences of the thermal diffusivities predicted with this equation were in the range of ${\pm}0.8\%$ compared with the practical values. This equation and another predicting equation obtained from the previous study for the pork product without fish meat could be simplified as following one equation, and the maximal differences of the thermal diffusivities predicted with this equation for both products with and without fish meat were in the range of less than ${\pm}2.5\%$$${\alpha}_p=(2.290+0.54{\cdot}X_w){\cdot}{\alpha}_w+0.0024{\cdot}10^{-6}{\cdot}X_w-0.3535{\cdot}10^{-6},(m^2{\cdot}s^{-1})$$
Kim, Yong-Seog;Jeon, Yong-Bae;Choi, Hae-Jin;Kim, Song-Mun;Kim, Sung-Min
The Korean Journal of Pesticide Science
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v.10
no.2
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pp.76-83
/
2006
In order to investigate the impacts of non-selective herbicide, glufosinate-ammonium (ammonium 4-[hydroxy(methyl)phosphinoyl] -DL-homoalaninate, GLA) to the non-target organisms, earthworm was exposed to GLA in the field soil for a month, and microbial populations in the soil were investigated after application of GLA. Simultaneously, the residues of GLA and its metabolite, 3-MPP were analyzed in the same soil. Meanwhile, to elucidate the influence of GLA to the growth of non-target crops incase of inter-furrow application, the amounts of carotenoid, chlorophyll, amino acid, proteins and sugars in the leaves of potato and chinese cabbage grown in the same field were investigated. In result, the dead earthworm was not observed during the test period, and the increasing rates of bodyweight were $9.410{\sim}11.603%$ in GLA-treated plots and 5.645% in GLA-untreated plots. The populations of fungi, bacteria and actinomycetes in the GLA-treated soils were $6.2{\times}10^4$, $1.5{\times}10^6$ and $5.7{\times}10^4$, respectively. They maintained relatively similar levels to the control which were $3.7{\times}10^4$, $3.7{\times}10^5$ and $3.7{\times}10^4$, respectively. In residue analysis, the limit of detection of GLA was 0.02 mg $kg^{-1}$, that of 3-MPP was the same level, and the half-life of GLA was 15 days in sandy clay loam soil. This result indicates that GLA was degraded very quickly in field soil. On the other hand, the amounts of physiological, biochemical components such as carotenoid, amino acid, chlorophyll, protein and sugar were ranged from 90.0 to 104.3% in potato and from 99.0 to 112.7% in chinese cabbage. Comparing with hand-weeded plots, it is indicated that GLA had not affected to the growth of non-target crops when applied at inter-furrow in crops-growing field.
Yu, Ri;Kwon, Young Sam;Oh, Tae-Ho;Kim, Tae-Hwan;Park, Sang-Joon
Journal of Veterinary Clinics
/
v.30
no.5
/
pp.339-345
/
2013
Mitogen-activated protein kinases (MAPKs) are a family of central signaling molecules that respond to numerous stimuli and are known to participate in processes of cell survival and death. However, it is not clear on data for cell-type specific activation of MAPKs in the progression of gastric ulcer. In the present study, we assessed how MAPKs localized at various cell types during the progression of gastric ulcer induced by ibuprofen. Gastric ulcer was induced by the repeated treatment of 200 mg/kg ibuprofen with 8 hrs interval in a day. Animals were sacrificed at 24 hrs, 48 hrs, and 72 hrs after oral treatment of ibuprofen and gastric tissues were subjected to immunohistochemical and immunoblotting evaluation. Immunoreactivity of phospho-extracellular signal-regulated kinase (p-ERK) was mainly expressed at the proliferating zone of gastric mucosa in control rats. But, these signals for p-ERK were highly shifted from cells of proliferating zone to parietal cells of the basal regions 24 hrs after treatment of ibuprofen. p-ERK signal was strongly expressed in epithelial cells adjacent to ulcer margin and new capillary and infiltrated inflammatory cells within granulation tissue of the ulcer base above 48 hrs after treatment of ibuprofen. While, phospho-c-Jun $NH_2$ terminal kinase (p-JNK) was mainly localized to the nuclei of the surface epithelial cells and the glandular epithelial cells in early gastric injury. Also, p-JNK was often observed as a scattered pattern in different regions of gastric mucosa with early gastric injury. Gradually, signal of p-JNK was strongly stained in infiltrated inflammatory cells and fibroblasts within severe ulcer base. Phospho-p38 (p-p38) MAPK was observed as scattered pattern within connective tissues of gastric mucosa. Especially, p-p38 MAPK showed strong signal in infiltrated macrophages within ulcer base. These results show that each MAPK has a specific role in various cell types during the progression of gastric ulcer.
Journal of the Korean Society of Food Science and Nutrition
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v.35
no.2
/
pp.199-204
/
2006
This study was conducted to investigate the quality characteristics of noodles prepared with the addition of nanofiltered (NF) powder of sunmul. Noodles were prepared with different levels $(0\%,\;1.5\%,\;3\%\;and\;5\%,\;w/w)$ of NF powder and physico-chemical properties were examined. Results of rapid visco analyzer showed that peak, trough, final viscosity and set back decreased as the NF powder level increased. The weight and volume of cooked noodles increased with the addition of NF powder. Turbidity of soup also increased as the amount of NF powder increased, indicating higher cooking loss. The color of wet and cooked noodles became greenish yellow as the NF powder level increased. Hardness, springiness, gumminess and brittleness of cooked noodles decreased with the increasing amount of NF powder. Results of sensory evaluation showed that noodles prepared with up to $3\%$ addition of NF powder was considered to be as acceptable as noodles prepared without NF powder.
The effects of diet composition, reproductive condition and ketonuria and ketosis on the composition of rumen volatil fatty acids(VFA) and the blood lipid concentration were investigated in Holstein dairy cows. Six healthy cows(Group I), 9 ketonuric cows (Group II) and 8 ketotic cows(Group III) were examined at days 13-15 pre-partum(PRP) and at day 15 post-partum(POP). The ratios of concentrated feed supplied at PRP and POP were 0% and 15%, respectively, in Group I, 25% and 67% in Group II, and were 30% and 46% in Group III. The fulfillment rates of total digestive nutrient(TDN) and digestive coarse protein(DCP) increased at POP in each group. Although total VFA concentrations were lower at POP than at PRP in each group, the concentrations at PRP and POP were higher in Group I than in Groups II and III. The molar ratio of acetic acid decreased at POP in Group I, while the ratio increased at POP in Groups II and III. The molar ratio of propionic acid increased at POP in Group I, while it decreased in Groups II and III. Blood total cholesterol, free cholesterol, cholesterol esters, HDL-cholesterol, phospholipid and free fatty acid concentrations decreased at POP in all three groups, but these levels were low in Group I, high in Group III and intermediate in Group II at PRP and POP. Triglyceride concentration decreased after parturition in these three groups, but the decrease was most striking conspicuous in Group III.
A total of 30 pigs (average 60kg) were used to investigate the influence of breeds on meat quality. Pigs were alloted into one of three experimental groups [A (Landrace×Yorkshire×Duroc), B (England Berkshire), and C (Kagoshima Berkshire)]. Pigs were slaughtered at approximately 110kg of live weight for measurement of meat qualities. Crude protein was significantly higher in B than those of other pig breed groups. Shear force value was higher and pH was lower in A than those of other pig breeds. In color, C was significantly higher in a* of meat color and lower in b* of fat color and in brittleness, hardness, springiness, chewiness as compared with other pig breeds. Essential fatty acid were significantly higher in A and lower in B than those of other pig breeds. Aspartic, threonine, serine, glutamic and phenylalanine were higher in C, whereas glycine, alanine, cystine, valine, leucine, tyrosine, histidine, lysine and arginine were significantly higher in A. In sensory evaluation of fresh meat, color was significantly higher in C. However, moisture, crude fat, crude ash and drip loss, marbling score, overall acceptability of fresh meat and all items in sensory evaluation of cooked meat were not significant difference among the pig groups. In conclusions, loin from Kagoshima Berkshire had the lowest shear force and the highest a* value. In the current study few effects of pig breeds were observed. Further studies are needed on the relationship between physico-chemical properties and the pig breeds.
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