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Development of Herbicide(Paraquat) Tolerant Plant Through Tissue Culture- 1. Mechanism of Plant Tolerance to Paraquat (농약(제초제)(農藥(除草劑)) Paraquat에 대한 저항성(抵抗性) 식물체(植物體) 선발육성(選拔育成)- 제1보(第1報) Paraquat에 대한 식물(植物)의 내성기작(耐性機作))

  • Kim, K.U.;Kim, D.U.;Kwon, S.T.
    • Korean Journal of Weed Science
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    • v.6 no.2
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    • pp.191-200
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    • 1986
  • The study was conducted to screen paraquat-tolerant plant species among crops and weeds, using the response of plant like leaf disc discoloration, visual injury and dry weight in the presence of paraquat. Mechanism of paraquat-tolerance was investigated in strains of soybean through evaluating activities of superoxide dismutase and peroxidase and the multiplication of callus derived from soybean cotyledon. In crops, Kwanggyo has been selected as a paraquat-tolerant variety among soybean cultivars tested, and Hood as a susceptible one. In weeds, Polygonum aviculare, Chenopodium album and Pinellia ternata were evaluated as the paraquat resistant species, providing the possibility for the donor plant species for paraquat resistance. Activity of superoxide dismutase known to detoxify paraquat was markedly greater in Kwanggyo, a paraquat-tolerant cultivar than in Hood, a susceptible one. In addition, the similar response like superoxide dismutase was observed in peroxidese activity. The greater inhibition of callus multiplication was determined in Hood, a susceptible one than a tolerant one, Kwnggyo. Based on all the informations, it is strongly proposed that paraquat tolerance in soybean is due to destruction of $O_2^-$ by elevated concentration of superoxide dismutase in the tolerant cultivar.

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Pest species, Damages and Seasonal Occurrences on Greenhouse Cultivated Asparagus in Jeju, Korea (제주도지역 아스파라거스의 주요 해충 종류, 피해 및 발생양상)

  • Choi, Kyung San;Song, Jeong Heub;Yang, Jin Young;Choi, Hwalran;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.231-237
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    • 2014
  • This study was carried out to investigate the pest species and their damage on Asparagus officinalis. Field surveys were conducted in asparagus greenhouse in Jeju, for four years from 2007 to 2010. Total of 16 pest species belonging to 5 orders were found as follows: Thrips tabaci, Thrips hawaiiensis, Adoxophyes honmai, Spodoptera litura, Spodoptera exigua, Helicoverpa armigera, Mamestra brassicae, Ascotis selenaria, Ectropis excellens, Geisha distinctissima, Aphis gossypii, Myzus persicae, Anomala albopilosa, Crioceris quatuordecimpunctata, Acusta despecta sieboldiana and Deroceras reticulatum. Among them, T. tabaci frequently occurred and damaged asparagus plants during the whole growing seasons. C. quatuordecimpunctata and snails caused severe damages on asparagus shoots in spring harvest season. In mid-late seasons, aphids and lepidopteran species occurred and attacked asparagus plants. Of the lepidopteran pests, S. litura, S exigua and H. armigera belonging to Noctuidae occurred moderately and fed on asparagus plants. And the pest control strategy in asparagus cultivation was discussed based on the present results.

The Effects of Eucommiae Cortex Pharmacopuncture Injected at Sinsu($BL_{23}$) on Neuropathic Pain in Rats (신유(腎兪)($BL_{23}$) 두중약침(杜仲藥鍼)이 흰쥐의 신경병리성 동통모델에 미치는 영향)

  • Lee, Dong Geun;Lee, Ook Jae;Lee, Ju Hee;Lee, Sang Hyun;Lee, Jung Hun;Shin, Jeong Cheol;Kim, Jae Hong
    • Journal of Acupuncture Research
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    • v.30 no.4
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    • pp.69-78
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    • 2013
  • Objectives : The purpose of this study is to examine whether Eucommiae Cortex pharmacopuncture may affect to the neuropathic pain in a rat model. Methods : To produce the model of neuropathic pain, under isoflurane 2.5 % anesthesia, underwent tight ligation by 6.0 silk thread and transection of the tibial and sural nerves, leaving the common peroneal nerve intact. After neuropathic surgery, the author examined if the exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the plantar with Dynamic Plantar Aesthesiometer. Three days after the neuropathic surgery, Eucommiae Cortex pharmacopuncture was injected at Sinsu($BL_{23}$) once every week for 6 weeks. After that, the author examined the withdrawal response of neuropathic rats' leg by Dynamic Plantar Aesthesiometer. And also the author examined Bax, Bcl-2, Bax/Bcl-2 ratio in the spinal cord of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats. Results : 1. The Eucommiae Cortex pharmacopuncture decreased the withdrawal response of mechanical allodynia that assessed with Dynamic Plantar Aesthesiometer in EC2-$BL_{23}$ group as compared with control group. 2. The Eucommiae Cortex pharmacopuncture decreased Bax/Bcl-2 ratio in EC1-$BL_{23}$, EC2-$BL_{23}$ group. But The Eucommiae Cortex pharmacopuncture injected at Sinsu($BL_{23}$) didn't change Bax, Bcl-2 expression level in the all group. 3. The Eucommiae Cortex pharmacopuncture decreased WBC count in EC1-$BL_{23}$, EC2-$BL_{23}$ group. Conclusions : We have noticed that Eucommiae Cortex pharmacopuncture decreased mechanical allodynia in the model of neuropathic pain compared with the control group. Bax/Bcl-2 ratio in spinal cord of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of neuropathic pain.

The Effect of Venesection with Cupping Therapy at Jeonjung($CV_{17}$) on the Heart Rate Variability in Healthy Adults (전중혈(膻中穴)($CV_{17}$) 습부항요법이 정상 성인의 심박변이도(HRV)에 미치는 영향)

  • Kim, Seong Joung;Park, Jin Soo;Lee, Jeong Ju;Park, Ok Ju;Kim, Sul Gi;Jeong, Ho Hyun;Park, Min Cheol;Kwon, Young Mi;Jo, Eun Heui
    • Journal of Acupuncture Research
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    • v.30 no.4
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    • pp.15-24
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    • 2013
  • Objectives : This study was accomplished to investigate the effects of venesection with cupping therapy at Jeonjung($CV_{17}$) on the heart rate variability(HRV) in healthy adults. And based on that, we tried to find out how venesection with cupping therapy at Jeonjung($CV_{17}$) affects the activity and the balance of the autonomic nervous system. Methods : We investigated on 60 healthy volunteers consisted of 30 subjects in venesection with cupping therapy group and 30 subjects in control group. The study was established by randomized trial. venesection with cupping therapy was applied at Jeonjung($CV_{17}$) for 10 minutes in venesection with cupping therapy group. No treatment was executed in control group. We measured HRV 3 times : First, baseline measurement and two more times after treatment in venesection with cupping therapy group(after rest in control group). The SPSS 12.0 for Windows was used to analyze the data by paired t-test(in both group), independent-test(between the groups) and Chi-square test(between the sexs). Results : 1. In venesection with cupping therapy group, SDNN, Ln(TP), Ln(LF), Normalized LF, and LF/HF ratio increased significantly. Normalized HF decreased significantly. Mean HRT, RMSSD, PSI, Ln(HF), and Ln(VLF) were not affected. 2. In control group, Ln(HF) decreased significantly. Mean HRT, SDNN, RMSSD, PSI, Ln(TP), Ln(LF), Ln(VLF), Normalized LF, Normalized HF, and LF/HF ratio were not affected. Conclusions : This study suggests that venesection with cupping therapy at Jeonjung($CV_{17}$) increases the activity of the autonomic nervous system and has effects on the balance of the autonomic nervous system.

A Study of Weight Control Attempt, Psychosocial Status, Nutrition Behavior and Related Factors among Female University Students

  • Kim, Kyung-A;Kang, Hyun-Joo;Kim, Kyungwon
    • Journal of Community Nutrition
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    • v.1 no.2
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    • pp.108-118
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    • 1999
  • This study was designed to examined psychosocial status and nutritional factors(nutrition knowledge, dietary attitudes, eating behavior, dietary intakes) among female university students and to investigate if there were differences in these variables by weight loss attempt. A cross-sectional survey was conducted to 225 female university students in Seoul. Survey instrument was adapted or modified based on literature review, and dietary intakes were assessed using 24-hour recall and CAN-pro. Descriptive statistics, t-test x$^2$-test were used in data analysis. Mean height, weight of subjects were 162.7cm, 51.3kg, and mean BMI was 19.4. One hundred-nine students(48.4%) were grouped into weight loss attempt roup and 116 students were grouped int non-attempt group. According to BMI, 58.7% of weight loss attempt group were underweight, suggesting that unnecessary weight control is common in college women. With respect to psychosocial status, subjects received moderate degree of stress and were slightly satisfied with life. There was no significant difference in stress, social support or self-esteem between the two groups, however, students in non-attempt group were more satisfied with their life(p<0.01) and adapted better in school that students in weight loss attempt group. Subjects scored 14.9${\pm}$2.1 (highest score : 20) on nutritional knowledge, and showed favorable dietary attitudes. Overall, nutritional knowledge and dietary attitudes were not significantly different between the two groups. Common eating problems were irregular meals(71.6%), followed by unbalanced meals and eating-out. Subjects in weight loss attempt group were more likely to change eating habit after entering the university and showed more undesirable eating habit. Dietary intake data indicated hat the energy derived from fat was slightly higher than the recommended level. Most of the nutrient intake was lower than the RDA, particularly, iron and calcium intake was below the 50% of the RDA. Although this study did not reveal significant difference in nutritional knowledge or dietary intake by weight loss attempt, this study showed status of weight control, eating habit and dietary intake in female university students, and provide some information for nutrition education of college women.

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The effect of oral breast milk on pain response of the neonates during heel lancing (모유경구투여가 발뒤꿈치 천자 시 신생아의 통증반응에 미치는 효과)

  • Kim, Kyunghwa;Park, Youngim;Kim, Taeim
    • Journal of the Korean Data and Information Science Society
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    • v.27 no.1
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    • pp.203-215
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    • 2016
  • This study was performed in order to investigate the effect of breast milk on pain relief in newborn during heel lancing. Nonequivalent control group pretest-posttest design by double blinded experiment was used. 50 neonates were randomized to receive breast milk (experimental group, n=25) or no treatment (control group, n=25) before undergoing heel lancing. Informed consent was obtained from parents of 50 neonates. Neonatal infant pain scale (NIPS), respiration rate, heart rate, oxygen saturation, and crying duration were used to assess subjects' pain reaction. Data were analyzed using SPSS 18.0. The experimental and control group showed a statistically significant differences in NIPS score (F=28.89, p<.001), heart rate (F=14.03, p<.001), respiration rate (F=4.79, p=.001), oxygen saturation (F=2.69, p=.027), and crying duration (t=-8.78, p <.001) at each time points (during heel lancing, right after heel lancing, 1, 2, 3 minutes after heel lancing). The result suggest that oral administration of expressed breast milk is safe and natural agents for reducing procedural pain of neonates. Another clinical trial study with more samples is recommended.

A clinical study on patients with Cold Hypersensitivity on hands and feet and their autonomic nervous system function using of Heart Rate Variability(HRV) (수족냉증 환자의 실태 및 HRV(Heart Rate Variability)를 이용한 자율신경계 기능 평가 연구)

  • Kim, Eun-Sook;Hwang, Jae-Ho;Lee, Jin-Moo;Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub;Choi, Jae-Ho
    • Journal of Oriental Medical Thermology
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    • v.5 no.1
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    • pp.1-13
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    • 2006
  • Objectives : This study was designed to analyze basic data for cold hypersensitivity patients with a questionnaire and investigate correlation between cold hypersensitivity and Heart Rate Variability(HRV). Methods : 49 patients who complain of cold hypersensitivity on hands and feet in ambulatory care were investigated in Oriental Gynecology, Kyunghee Oriental Medical Center and Women medical center, Kangnam Koreana hospital from May 1, 2006 to October 20, 2006. All patients were asked to answer a questionnaire. After careful I examination to rule out other disease which may affect Digital Infrared Thermal Imaging(DITI) and HRV data, patients were taken thermography for the diagnosis of cold hypersensitivity Based on the result of thermography, the patients were divided into two groups(Objective cold hypersensitivity and Subjective cold hypersensitivity). Then, these two groups were compared using HRV data which was measured in the supine position for 5 minutes. Results: 1. For most patients, cold hypersensitivity first developed during puberty. 2. The parts of the body that felt cold first time were hands and feet. 3. The cold sensation was increased in winter or at bedtime Showing that the sensation can be changed according to the level of coldness. 4. Among women who sufferfrom cold hypersensitivity, very few of them were treated. 5. More than half of cold hypersensitivity patients's family member also had a cold hypersensitivity. 6. The decrease in mean values of LF/HF ratio was observed in objective cold hypersensitivity group than subjective cold hypersensitivity group and the decrease was significant(P=0.014) when examined by Student t-test. Conclusions : It is necessary that the cold hypersensitivity patients should be treated carefully considering the facts mentioned above. And it can be suggested that dysautinomia be related with cold hypersensitivity and be evaluated by HRV.

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Anti-inflammatory effect of seed oil of Schisandra chinensis in the LPS-treated RAW 264.7 macrophages (LPS로 자극된 Raw 264.7 대식세포에서 오미자 씨앗오일의 항염증 효과)

  • Jang, Jae-Yoon;Park, Geun-Hye
    • The Korea Journal of Herbology
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    • v.30 no.6
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    • pp.77-82
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    • 2015
  • Objectives : This study was designed to investigate of the anti-inflammatory effects of Schisandra chinensis seed oil(SSO) on the production of pro-inflammatory substances in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages.Methods : SSO was measured the production of pro-inflammatory factor (NO, PGE2, IL-1β iNOS and, COX-2) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. we used the following methods : cell viability assay, Griess reagent assay, enzyme-linked immunosorbent assay, Western blotting analysis.Results : The cell viability of SSO(0∼500 μl/mL) processing group was 96.9% and the processing of SSO didn't have an effect on the cytotoxicity. The inhibitory effect of the nitric oxide (no) production of SSO(500 μg/mL, 50 μg/mL, 10 μg/mL) was each 70.3%, 37.6% and 26.5%. IL-1β production inhibition ability of SSO(500 μg/mL, 100 μg/mL) was each 49.88% and 48.8%. PGE2 production inhibition ability of SSO(500 μg/mL, 100 μg/mL) was each 49.88% and 73.1%, 70.5%. By using SSO, it experimented about iNOS protein expression inhibition ability, that is the NO production enzyme. iNOS protein expression increased in the group processing LPS independently. iNOS protein expression decreased in the group processing SSO together. The expression of the COX-2 protein decreased 89.6%, 81.8% in the group processing SSO. The significance was in the relationship with NO formation inhibition with the relationship with the PGE2 formation inhibition and iNOS protein, it confirmed in SSO with the COX-2 protein.Conclusions : Stimulation of the RAW 264.7 cells with LPS caused an elevated production of nitric oxide (NO), IL-1β and PGE2 which was markedly inhibited by the pretreatment with SSO without causing any cytotoxic effects. The reduced expressions of iNOS protein were consistent with the reductions in NO production in the culture media. SSO may be useful for the treatment of various inflammatory diseases.

The Effect of Pre and Intra-Operative Warming Therapy on Tympanic Temperature Changes during Perioperative Phase in Receiving Patients with Total Hip Replacement (수술전과 수술중 피부가온요법 적응이 고관절 전치환술 환자의 수술 주기 고막체온 변화에 미치는 영향)

  • Kwon Young-Sook;Kim Eun-Hee
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.7 no.1
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    • pp.86-96
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    • 2000
  • The purpose of the study was to investigate the effects of Warming Therapy used with patients consistantly before and during surgery to on changes in their body temperatures. The data were collected from patients in a university hospital in Taegu between December 1, 1998 and May 31, 1999. The subjects were selected from patients who were hospitalized for total hip replacement surgery. Thirty participants were assigned to two groups : experimental(Warming Therapy) group and control group. Each group consisted of 15 patients. The research design was a repeated measurement design, using a nonequivalent control group. The Warming Therapy, using a forced-air warming blanket, that is a, 'Bair Hugger' was applied to subjects in the experimental group. The subjects in the group were treated with the 'Bair Hugger' to warm up the whole body for 40 minutes before surgery and upper body and face during the operation. The core temperature was measured using a tympanic thermometer. The body temperature of the patients was measured 13 times every 15 minutes during the surgery. After the operation the body temperature of the patients was measured 4 times every 15 minutes, from the time of arrivial in the recovery room to the time of leaving the recovery room. The SPSS Win 9.0 program was used for data analysis. Specific methods tested were done using ${\chi}^2-test$, t-test, repeated measures ANOVA. The findings of the study are as follows. 1. The first hypothesis, 'The level of tympanic temperature for the experimental group which received Warming Therapy will be higher than that of the control group during the operation', was supported (F=32.16, p=.000). 2. The second hypothesis, 'The level of tympanic temperature for the experimental group which received Warming Therapy will be higher than that of the control group after the operation', was supported.(F=33.36, p=.000) 3. During recovery, shivering was observed one patient in the experimental group and seven patients in the control group. In summary, the findings of the study suggest that the 'Warming Therapy' applied before and during the surgery was a very effective treatment for surgical patients in maintaining the core temperature during surgery

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Axial Pressing Method Using Pulse Magnetizing Field for the Preparation of Nd-Ee-B Sintered Magnets (펄스자장을 이용한 고이방화 Nd-Fe-B자석의 종축자장성형방법)

  • Kim, Dong-Hwan;Kang, Byung-Kil;Jang, Dong-Youl;Kim, Andrew S.;Kim, Sang-Myun;Jang, T.S.
    • Journal of the Korean Magnetics Society
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    • v.13 no.4
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    • pp.182-186
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    • 2003
  • A new axial pressing method using pulse magnetizing field was studied to improve the remanence of Nd-Fe-B sintered magnets. In order to make near-net shape green compacts of butterfly, disk, or coin magnets, conventional axial-type pressing has been normally used. However, compared to the transverse-type pressing, it is not possible to obtain higher remanence by this method because the magnetic alignment of powder begins to deteriorate when the density of green compacts increases over a critical value. On the other hand, we found that an axial pressing under pulse magnetizing field was very effective to increase the degree of magnetic alignment of powder, yielding remanences even higher than those obtained by the transverse pressing. In this study, it was revealed that appropriate tapping density and how to apply pulse magnetic held were important to improve the grain alignment and thus remanence of Nd-Fe-B sintered magnet.