• Molecules and Cells
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• 제19권3호
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• pp.334-341
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• 2005

Plants are sessile and rely on a wide variety of growth hormones to adjust growth and development in response to internal and external stimuli. We have identified a gene, designated NAN, encoding a basic helix-loop-helix (bHLH) transcription factor that regulates cell elongation and seed germination in plants. NAN has an HLH motif in its C-terminal region but does not have any other discernible homologies to bHLH proteins. A bipartite nuclear localization signal is located close to the HLH motif. An Arabidopsis mutant, nan-1D, in which NAN is activated by the insertion of the 35S enhancer, exhibits growth retardation with short hypocotyls and curled leaves. It is also characterized by reduced seed germination and apical hook formation, symptomatic of GA deficiency or disrupted GA signaling. The phenotypic effects of nan-1D were increased by treatment with paclobutrazol (PAC), an inhibitor of gibberellic acid (GA) biosynthesis. NAN is constitutively expressed throughout the life cycle. Our observations indicate that NAN has a housekeeping role in plant growth and development, particularly in seed germination and cell elongation, and that it may modulate GA signaling.

• 한국건설관리학회논문집
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• 제17권3호
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• pp.32-42
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• 2016

현재 국내 대부분의 토목 건축 구조물이 BIM 정보가 부재한 상황에서 준공 BIM(as-built BIM)의 수요가 점차 증가하고 있다. 준공 BIM 구축을 위한 공간자료 취득에는 고밀도의 포인트 클라우드를 생성할 수 있는 레이저 스캐너가 주로 활용되고 있다. 하지만 기존의 고정식 스캔 시스템은 이동이 번거롭고, 정밀한 위치 선정이 필요 하며, 스캔 자료 정합을 위해 별도의 표지를 설치하거나 공액점을 추출하는 과정이 필요하다. 본 연구에서는 수작업을 최소화하기 위해 기존의 고정식 스캔 시스템을 대체할 수 있는 이동식 스캔 시스템을 제안하고자 하며, 기반 기술로 graph-based SLAM을 적용하였다. 테스트 장비는 총 세 개의 2차원 스캐너를 탑재하고 있으며, 중앙의 한 개는 수평으로 설치되어 graph 구축을 통한 이동경로취득에 사용되었고, 좌우 두 개는 수직으로 설치되어 시스템 진행의 연직 방향으로 주변 구조물에 대한 3차원 스캔 정보 취득에 사용되었다. 개발된 graph-based SLAM은 이동경로 상에 누적된 위치오차를 해소하기 위한 loop closure 처리 방법으로 Adaboost 기계학습을 적용하였다. 이는 특히 본 연구에서 사용한 장비와 같이 기계학습을 위한 다수의 feature 정보를 제공할 수 있는 멀티 스캐너 시스템에 적합한 방식이며, 두 실내공간을 대상으로 한 테스트에서 단일 스캐너 대비 false positive rate를 각각 7.9% 및 13.6%까지 줄일 수 있었다. 최종적으로 연구대상지역의 2차원 및 3차원 지도 구축을 통해 개발된 graph-based SLAM의 효용성을 확인하였다.

• 인터넷정보학회논문지
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• 제21권3호
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• pp.113-121
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• 2020

Human Pose Estimation (HPE) which localizes the human body joints becomes a high potential for high-level applications in the field of computer vision. The main challenges of HPE in real-time are occlusion, illumination change and diversity of pose appearance. The single RGB image is fed into HPE framework in order to reduce the computation cost by using depth-independent device such as a common camera, webcam, or phone cam. However, HPE based on the single RGB is not able to solve the above challenges due to inherent characteristics of color or texture. On the other hand, depth information which is fed into HPE framework and detects the human body parts in 3D coordinates can be usefully used to solve the above challenges. However, the depth information-based HPE requires the depth-dependent device which has space constraint and is cost consuming. Especially, the result of depth information-based HPE is less reliable due to the requirement of pose initialization and less stabilization of frame tracking. Therefore, this paper proposes a new method of HPE which is robust in estimating self-occlusion. There are many human parts which can be occluded by other body parts. However, this paper focuses only on head self-occlusion. The new method is a combination of the RGB image-based HPE framework and the depth information-based HPE framework. We evaluated the performance of the proposed method by COCO Object Keypoint Similarity library. By taking an advantage of RGB image-based HPE method and depth information-based HPE method, our HPE method based on RGB-D achieved the mAP of 0.903 and mAR of 0.938. It proved that our method outperforms the RGB-based HPE and the depth-based HPE.

• The Korean Journal of Physiology and Pharmacology
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• 제18권2호
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• pp.155-161
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• 2014

Overexpression of amyloid precursor protein with the Swedish mutation causes abnormal hyperphosphorylation of the microtubule-associated protein tau. Hyperphosphorylated isoforms of tau are major components of neurofibrillary tangles, which are histopathological hallmarks of Alzheimer's disease. Protein phosphatase 2A (PP2A), a major tau protein phosphatase, consists of a structural A subunit, catalytic C subunit, and a variety of regulatory B subunits. The B subunits have been reported to modulate function of the PP2A holoenzyme by regulating substrate binding, enzyme activity, and subcellular localization. In the current study, we characterized regulatory B subunit-specific regulation of tau protein phosphorylation. We showed that the PP2A B subunit PPP2R2A mediated dephosphorylation of tau protein at Ser-199, Ser-202/Thr-205, Thr-231, Ser-262, and Ser-422. Down-regulation of PPP2R5D expression decreased tau phosphorylation at Ser-202/Thr-205, Thr-231, and Ser-422, which indicates activation of the tau kinase glycogen synthase kinase 3 beta ($GSK3{\beta}$) by PP2A with PPP2R5D subunit. The level of activating phosphorylation of the $GSK3{\beta}$ kinase Akt at Thr-308 and Ser-473 were both increased by PPP2R5D knockdown. We also characterized B subunit-specific phosphorylation sites in tau using mass spectrometric analysis. Liquid chromatography-mass spectrometry revealed that the phosphorylation status of the tau protein may be affected by PP2A, depending on the specific B subunits. These studies further our understanding of the function of various B subunits in mediating site-specific regulation of tau protein phosphorylation.

• 한국산업융합학회 논문집
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• 제24권5호
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• pp.597-608
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• 2021

The control head components used in small military vessels are designed to be domestically produced, prototypes, structural analysis, and casting methods are designed and cast. The control head assembly consists of a lever, an aluminum outside cover, Middle, front gear cover, back gear cover, and a zinc worm gear. In order to reverse the design of each component, 3D scanning device was used, 3D modeling was performed by CATIA, and prototype productions were carried out by 3D printer. In order to reduce the cost of components, gating system is used by gravity casting method. The SRG ratio of 1:0.9:0.6 was set by applying non-pressurized gating system to aluminum parts, 1:2.2:2.0 and pressurized gating system to zinc parts, and the shapes of sprue, runner and gate were designed. The results of porosity were also confirmed by casting analysis in order to determine whether the appropriate gating system can be designed. The results showed that all parts started solidification after filling completely. ANSYS was used for structural analysis, and the results confirmed that all five components had a safety factor of 15 more. All castings are free of defects in appearance, and CT results show only very small porosity. ZnDC1 zinc alloy worm gear has a tensile strength of 285 MPa and an elongation of 8%. The tensile strength of the four components of A356 aluminum alloy is about 137-162 MPa and the elongation is 4.8-6.5%.

• Healthcare Informatics Research
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• 제24권4호
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• pp.394-401
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• 2018

Objectives: Augmented reality (AR) technology has become rapidly available and is suitable for various medical applications since it can provide effective visualization of intricate anatomical structures inside the human body. This paper describes the procedure to develop an AR app with Unity3D and Vuforia software development kit and publish it to a smartphone for the localization of critical tissues or organs that cannot be seen easily by the naked eye during surgery. Methods: In this study, Vuforia version 6.5 integrated with the Unity Editor was installed on a desktop computer and configured to develop the Android AR app for the visualization of internal organs. Three-dimensional segmented human organs were extracted from a computerized tomography file using Seg3D software, and overlaid on a target body surface through the developed app with an artificial marker. Results: To aid beginners in using the AR technology for medical applications, a 3D model of the thyroid and surrounding structures was created from a thyroid cancer patient's DICOM file, and was visualized on the neck of a medical training mannequin through the developed AR app. The individual organs, including the thyroid, trachea, carotid artery, jugular vein, and esophagus were localized by the surgeon's Android smartphone. Conclusions: Vuforia software can help even researchers, students, or surgeons who do not possess computer vision expertise to easily develop an AR app in a user-friendly manner and use it to visualize and localize critical internal organs without incision. It could allow AR technology to be extensively utilized for various medical applications.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2022년도 가을 학술논문 발표대회
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• pp.243-244
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• 2022

In this paper, experiments were conducted on signal amplification of polymer capsules for application to Ground Penetrating Radar so as to enable real-time monitoring of polymer capsules inside concrete using the Morphology Dependent Resonance phenomenon. A TEM CELL and a vector network analyzer were used to analyze the difference in resonance frequency depending on the material of the sphere and the presence or absence of fracture. In order to manufacture a capsule of a size that can be measured using millimeter waves used in GPR, we manufactured a capsule with a 3D printer and analyzed the effects of the presence or absence of coating and the size of the capsule on the resonance frequency. Resonant frequency or signal amplification is more affected by diameter than coating. The capsule showing the highest amplification is the resin-coated 50 mm diameter capsule with a 316-fold increase and the lowest capsule is the uncoated 10 mm diameter capsule with a signal amplification of 11.9 times. These results demonstrate the potential of GPR to measure the position and state of self-healing capsules, which are small-sized polymers, in real time using millimeter waves.

• Toxicological Research
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• 제14권2호
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• pp.123-127
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• 1998

BRCA1 is a tumor suppresser gene in familial cases of breast cancer. It has been controversial whether the subcellular localization of BRCA1 is located in nuclei or cytoplasm in normal human breast cells. We found that a p220 protein was expressed in Type II Normal human breast epithelial cells (NHBEC) but not in Type I NHBEC in Western blot analysis using the 17F8 (3A2) antibody. Immunostaining using the same antibody revealed positive staining in nuclei, cytoplasm and perinuclei of Type II cells and negative staining in Type I NHBEC. The p220 protein, however, was expressed in SV40 immortalized Type I NHBEC and tumorigenic cells derived from them after x-ray and neu oncogene treatment. The subcelluar localization was mostly cytoplasmic and punctate in the nuclei. The breast carcinoma cell lines, MCF-7 and T47D, also expressed the p220 protein. Using RT-PCR, we observed the expression of BRCA1 mRNA in both Type I and Type II NHBEC. This result indicated that there might be mechanisms involved in post-translational or translational regulation of BRCA1 gene. It is speculated that the absence of BRCA1 protein expression in Type I NHBEC might playa role in their susceptibility to neoplastic transformation.

• Smart Structures and Systems
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• 제20권4호
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• pp.397-413
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• 2017

Precast concrete (PC) members are currently being employed for general construction or partial replacement to reduce construction period. As assembly work in PC construction requires connecting PC members accurately, measuring the 6-DOF (degree of freedom) relative displacement is essential. Multiple planar markers and camera-based displacement measurement systems can monitor the 6-DOF relative displacement of PC members. Conventional methods, such as direct linear transformation (DLT) for homography estimation, which are applied to calculate the 6-DOF relative displacement between the camera and marker, have several major problems. One of the problems is that when the marker is partially hidden, the DLT method cannot be applied to calculate the 6-DOF relative displacement. In addition, when the images of markers are blurred, error increases with the DLT method which is employed for its estimation. To solve these problems, a hybrid method, which combines the advantages of the DLT and MCL (Monte Carlo localization) methods, is proposed. The method evaluates the 6-DOF relative displacement more accurately compared to when either the DLT or MCL is used alone. Each subsystem captures an image of a marker and extracts its subpixel coordinates, and then the data are transferred to a main system via a wireless communication network. In the main system, the data from each subsystem are used for 3D visualization. Thereafter, the real-time movements of the PC members are displayed on a tablet PC. To prove the feasibility, the hybrid method is compared with the DLT method and MCL in real experiments.

• 한국음향학회지
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• 제26권1호
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• pp.48-54
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• 2007

본 논문에서는 수중에서 이동체의 3차원 위치를 추정하기 위한 위치추적 시스템을 구현하였다. 본 연구에서는 네 개의 초음파 센서를 수중의 서로 다른 위치에 고정시키고, 이동중인 이동체 센서와 서로 다른 신호를 송 수신하게 함으로써 고정체와 이동체 모두에서 이동체의 3차원 위치 추적과 이동체의 원격제어를 가능하게 하였다. 또한 위치 추적 시에 Newton 알고리즘에서 매 iteration 시에 Jacobian 행렬의 norm을 추정하고, 행렬의 norm이 임계값 이상이 되어 역행렬에 의한 해가 불안정해질 때는 또 다른 초기값을 이용하여 해를 구하게 함으로써 이동체의 위치가 보다 신뢰성 있게 추정되게 하였다. 그리고 제안한 알고리즘을 이용하여 실시간 위치 추적이 가능한 위치 추적 DSP 시스템을 구현하였으며, 성능 검증을 위한 수조 실험을 수행하였다. 성능 검증결과 본 연구에서 구현한 위치 추적 시스템은 1초에 2회 이상의 속도로 오차율 5cm 이내에서 이동체의 위치를 추적함을 알 수 있었다.