• KSBB Journal
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• v.11 no.2
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• pp.165-172
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• 1996

To obtain a correctly folded human proinsulin, export of proinsulin using Staphylococcal protein A signal sequence-mediated secretion pathway has been attempted in E.coli. A secretion operon for proinsulin was constructed by consecutively connecting T7 promoter, SPA ribosome binding site, SPA signal sequence gene, and human proinsulin gene. Little immunoreactive proinsulin was detected in the periplasmic space and. culture medium, and not even in cytoplasmic space. The qualitative analysis of transcribed proinsulin mRNA and the in vitro transcription/translation experiment suggests that the negligible level of proinsulin export appears to be due to intracellular degradation of proinsulin, rather than due to the blockage during translocation. However, expression of proinsulin fusion protein such as MBP-proinsulin could dramatically increase export of proinsulin in E.coli.

• Journal of the Economic Geographical Society of Korea
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• v.8 no.2
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• pp.301-314
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• 2005

The purpose of this study is to inquiry specialized development strategies to revive the declining regional tourism in Japan, through the general characteristics of tourists and the evaluation of various tourism resources. Reaching its period of maturity after the bubble economy collapse, domestic tourism in Japan requires a change in quality of the regional tourism industry. The Kakeyu spa region, which has long history and abundant cultural resources, is specializing through rehabilitation strengthening theme of 'health seeking and recuperation'. The rate of the elderly women visitors is high in the Kakeyu spa, and the visitors use public transport and make a relatively long stay, because they want to recuperate their health or to cure their disease. In the evaluation of tourism resources, the visitors showed an increasing interest in the solemn temple scenery and the tranquil stroll road which are near by their lodgings. To reflect such demand characteristics, the Kakeyu community is actively developing the business such as rehabilitation, health-food development, voluntary service, and barrier free based on spa, culture, and nature.

• Food Science of Animal Resources
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• v.40 no.5
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• pp.758-771
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• 2020

This study was to investigate the prevalence and characteristics of antimicrobial-resistant Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from 4,264 retail meat samples including beef, pork, and chicken in Korea between 2013 and 2018. A broth microdilution antimicrobial susceptibility testing was performed for S. aureus. Molecular typing by multilocus sequence typing (MLST), spa typing, and pulsed-field gel electrophoresis (PFGE), was performed on mecA-positive S. aureus strain. S. aureus was isolated at a rate of 18.2% (777/4,264), of which MRSA comprised 0.7% (29 strains). MLST analysis showed that 11 out of the 29 MRSA isolates were predominantly sequence type (ST) 398 (37.9%). In addition, ST72, ST692, ST188, ST9, and ST630 were identified in the MRSA isolates. The spa typing results were classified into 11 types and showed a high correlation with MLST. The antimicrobial resistance assays revealed that MRSA showed 100% resistance to cefoxitin and penicillin. In addition, resistance to tetracycline (62.1%), clindamycin (55.2%), and erythromycin (55.2%) was relatively high; 27 of the 29 MRSA isolates exhibited multidrug resistance. PFGE analysis of the 18 strains excluding the 11 ST398 strains exhibited a maximum of 100% homology and a minimum of 64.0% homology. Among these, three pairs of isolates showed 100% homology in PFGE; these results were consistent with the MLST and spa typing results. Identification of MRSA at the final consumption stage has potential risks, suggesting that continuous monitoring of retail meat products is required.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.3
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• pp.87-99
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• 2009

We evaluated modified Soil-Plant-Atmosphere model's performance to simulate the seasonal variation of net ecosystem exchange (NEE) of carbon and examined the critical controlling mechanism on carbon exchange using the model over a deciduous forest at Gwangnung in 2006. The modified Soil-Plant-Atmosphere (mSPA) model was calibrated to capture the mean NEE during the daytime (1000-1400 LST) and used to simulate gross primary productivity (GPP). Ecosystem respiration ($R_e$) has been estimated using an empirical formula developed at this site. The simulation results indicated that the daytime mean stomatal conductance was highly correlated with daily insolation in the summer. Low stomatal conductance in high insolation occurred on the days with low temperature rather than with high vapor pressure deficit. It suggests that the forest rarely experienced water stress in the summer of 2006. The model captured the observed bimodal seasonal variation with a mid-season depression of carbon uptake. The model estimates of annual GPP, $R_e$ and NEE were $964\;gC\;m^{-2}\;yr^{-1}$, $733\;gC\;m^{-2}\;yr^{-1}$, and $-231\;gCm\;^{-2}\;yr^{-1}$, respectively. Compared to the observed annual NEE, the modeled estimates showed more carbon uptake by about $140\;gC\;m^{-2}\;yr^{-1}$. The uncertainty of the estimate of annual NEE in a complex terrain is discussed.

• Journal of Auto-vehicle Safety Association
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• v.14 no.1
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• pp.45-54
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• 2022

Recently, the introduction of Cooperative Intelligent Transport Systems (C-ITS) has been attempted to solve the limitation of only the sensor of the vehicle itself. For example, vehicles traveling at intersections can drive more safely through C-ITS. By using V2X communication of WAVE and LTE, the driver can receive the status and time of traffic lights. However, LTE has a larger transmission delay time than WAVE, so timimg data may not match in real time. In this paper, using the SPaT message, it was confirmed that LTE has a larger C-ITS service transmission delay time than WAVE. Finally, it was confirmed that the timing data of SPaT provided by LTE corrected by the algorithm is similar to SPaT provided by WAVE. It was confirmed that safer intersection driving is possible based on real-time.

• Korean Journal of Food Science and Technology
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• v.29 no.1
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• pp.38-43
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• 1997

Free phenolic acids (FPA), soluble phenolic acid esters (SPA) and insoluble-bound phenolic acids (IPA) were extracted from defatted Perillae semen flour and the antioxidative components in FPA extract was separated by column chromatography and HPLC. Total phenolic content of defatted Perillae semen flour was 0.38% as chlorogenic acid and each percent ratio of the content of FPA, SPA and IPA to total phenolic content was 71.1%, 15.8% and 13.1%, respectively. The antioxidative activity was compared by measuring of electron donating ability (EDA) and thiobarbituric acid value (linoleic acid substrates). The FPA extract was showed the highest antioxidative activity among the three kinds of phenolic extracts. The FPA extract showing the highest antioxidative activity was separated by silica gel column chromatography and then the separated fractions were compared in terms of antioxidative activity. The fractions of acetone : methanol (8 : 2) showing the highest antioxidative activity was further separated by HPLC. Five fractions (F-I, F-II, F-III, F-IV and F-V) were observed on the HPLC chromatogram and F-I fraction showed the highest antioxidative activity.

• Korean Journal of Veterinary Service
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• v.33 no.1
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• pp.45-50
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• 2010

Mycoplasma gallisepticum (MG) is major cause of chronic respiratory disease in chickens. M. synoviae (MS) most frequently occurs a subclinical upper respiratory infection but may result in airsacculitis and synovitis in chickens and turkeys. Both mycoplasmas induce economic losses by triggering chronic respiratory signs, airsacculitis and decreased egg production. For prevention of the infections, live attenuated andinactivated vaccines are commercially used for prevention of MG but not MS in Korea. Serum plate agglutination (SPA) and enzyme-linked immunosorbent assay (ELISA) have been commonly used for serological diagnosis for MG and MS. Recently, it is believed that MS spread in chickens is very seriously in Korea and respiratory infection with MS causes substantial loss in poultry farms. In this study, we investigated the serological prevalence of MG and MS in unvaccinated chickens between 2008 and 2009. The overall seroprevalence of MG was 24% of 2,094 for individual chickens and 24% of 189 farms. The overall seroprevalence of MS was 36% in 2,095 chickens and 39% in 198 farms. The results show that seropositive ratio of MS is higher than MG. The geographical prevalence of MG has been estimated in following sequence; Gangwon, Jeolla, Gyeonggi, Gyeongsang, and Chungcheong. The geographical prevalence of MS has been estimated as follows; Gangwon, Gyeonggi, Gyeongsang, Chungcheong, and Jeolla. Seasonal seroprevalencewas also examined, and it found that seroprevalence in spring, fall and winter was higher than that in summer in MG, but not in MS. No significant difference was shown in seroprevalence according to breed. Future study about pathogenicity of MS isolates would be needed and economical losses by MS outbreaks should be analyzed. Moreover, we compared sero-positivity obtained with SPA and ELISA. The kappa value of MG between SPA and ELISA was 0.8061 and the kappa value of MS between SPA and ELISA was 0.7649.

• International Journal of Advanced Culture Technology
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• v.7 no.1
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• pp.237-242
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• 2019

Spark variance technique is melded the jagged spill-over-sonance status of the glitter-differentiation knowledge level (GDKL) on the spark knowledge gestalt. The knowledge level condition by the spark knowledge gestalt system is comprised with the spill-over-sonance system. As to search a spot of the glitter situation, we are obtained of the spark value with black-red dot by the spill-over upper structure. The concept of knowledge level is comprised the reference of glitter-differentiation level for variance signal by the spark sonance gestalt. Further presenting a jagged variance of the GDKL of the maximum in terms of the spill-over-sonance gestalt, and spark spot sonance that was the a spark value of the far variance of the Spa-kg-FA-${\rho}_{MAXN}$ with $17.68{\pm}2.22units$, that was the a spark value of the convenient variance of the Spa-kg-CO-${\rho}_{MAXN}$ with $7.55{\pm}0.59units$, that was the a spark value of the flank variance of the Spa-kg-FL-${\rho}_{MAX}$ with $2.70{\pm}0.48units$, that was the a spark value of the vicinage variance of the Spa-kg-VI-${\rho}_{MAX}$ with $0.48{\pm}0.05units$. The spill-over sonance will be to appraisal at the jagged ability of the spill-over-sonance gestalt with black-red dot by the spark knowledge level on the GDKL that is presented the glitter-differentiation gestalt by the knowledge level system. Spill-over knowledge system will be possible to restrain of a gestalt by the special signal and to employ a spark data of spill-over sonance level.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.17 no.2
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• pp.115-123
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• 2007

In cryptographic devices like a smart-card whose computing ability and memory are limited, cryptographic algorithms should be performed efficiently. Scalar multiplication is very important operation in Elliptic Curve Cryptosystems, and so must be constructed in safety against side channel attack(SCA). But several countermeasures proposed against SCA are exposed weaknesses by new un-dreamed analysis. 'Double-and-add always scalar multiplication' algorithm adding dummy operation being known to secure against SPA is exposed weakness by Doubling Attack. But Doubling Attack cannot apply to sABS receding proposed by Hedabou, that is another countermeasure against SPA. Our paper proposes new strengthened Doubling Attacks that can break sABS receding SPA-countermeasure and a detailed method of our attacks through experimental result.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.18 no.2
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• pp.110-114
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• 2004

This paper deals with the dynamic characteristic analysis of linear actuator(LA) considered with coupling of mechanical spring system This application of linear actuator is increasing due to simple mechanical structure, a high efficiency, and a stable motional action. This paper proposed and analyzed the Serial Piston Actuator(SPA) by using mechanical spring system coupled with linear actuator through the simplified structure which was verified practical experiments.