• Title/Summary/Keyword: 2DEG

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Studies on Reserved Carbohydrates and Net energy Lactation ( NEL ) in Corn and Sorghum II. Synthesis and accumulation pattern of cell-wall constituents (옥수수 및 Sorghum에 있어서 탄수화물과 NEL 축적에 관한 연구 II. Cell-Wall Constituents 합성 및 축적형태)

  • ;G. Voigtlaender
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.2
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    • pp.127-135
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    • 1985
  • The effects of morphological development and environmental temperature on synthesis and accumulation behavior of cell-wall constituents were studied in maize cv. Blizzard and sorghum cv. Sioux and Pioneer 931 at Muenchen Technical University from 1979 to 1981. Various growth stages of maize and sorghum plants were grown on field and phytotron at 4 temperature regimes of 30/25, 25/20, 28/18 and 18/8 degree C and mid-summer sunlight over 13-hour days. The results are summarized as follow: 1. Cell-wall constituents in sorghum and maize plants were shown to have a great synthesis rates at early growth stage from growing point differentiation to final leaf visible. The highest concentration of cell wall contents were found at heading stage with 52-54% and 64-68% of neutral detergence fiber, and 30% and 45% of acid detergence fiber foe maize and sorghum, respectively. 2. The structural carbohydrates, cellulose and hemicellulose, were found as a main components of cell-wall constituents. Cellulose were mainly accumulated in stalks, while hemicellulose were an important cell wall components in leaves and panicle. 3. Synthesis rates of cell-wall constituents and non-strnctural carbohydrates were associated with increasing of temperature. Reserved carbohydrates such as fructosan, mono - and dissaccharose in plant were, however, declined when the temperature exceeded 30 deg C, during the accumulation of cellulose, hemicellulose and lignin were increased continuously. 4. Cell-wall constituents lowered digestibility and net energy accumulation in sorghum and maize plants. In a in vitro and in vivo trial, it was found a negative correlation between digestion dry matter and cell wall constituents, especially cellulose and lignin.

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Streamflow response to climate change during the wet and dry seasons in South Korea under a CMIP5 climate model (CMIP5 기반 건기 및 우기 시 국내 하천유량의 변화전망 및 분석)

  • Ghafouri-Azar, Mona;Bae, Deg-Hyo
    • Journal of Korea Water Resources Association
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    • v.51 no.spc
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    • pp.1091-1103
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    • 2018
  • Having knowledge regarding to which region is prone to drought or flood is a crucial issue in water resources planning and management. This could be more challenging when the occurrence of these hazards affected by climate change. In this study the future streamflow during the wet season (July to September) and dry season (October to March) for the twenty first century of South Korea was investigated. This study used the statistics of precipitation, maximum and minimum temperature of one global climate model (i.e., INMCM4) with 2 RCPs (RCP4.5 and RCP8.5) scenarios as inputs for The Precipitation-Runoff Modelling System (PRMS) model. The PRMS model was tested for the historical periods (1966-2016) and then the parameters of model were used to project the future changes of 5 large River basins in Korea for three future periods (2025s, 2055s, and 2085s) compared to the reference period (1976-2005). Then, the different responses in climate and streamflow projection during these two seasons (wet and dry) was investigated. The results showed that under INMCM4 scenario, the occurrence of drought in dry season is projected to be stronger in 2025s than 2055s from decreasing -7.23% (-7.06%) in 2025s to -3.81% (-0.71%) in 2055s for RCP4.5 (RCP8.5). Regarding to the far future (2085s), for RCP 4.5 is projected to increase streamflow in the northern part, and decrease streamflow in the southern part (-3.24%), however under RCP8.5 almost all basins are vulnerable to drought, especially in the southern part (-16.51%). Also, during the wet season both increasing (Almost in northern and western part) and decreasing (almost in the southern part) in streamflow relative to the reference period are projected for all periods and RCPs under INMCM4 scenario.

Species-specific Expression of Rpia Transcript in Cumulus-oocyte-complex (난자-난구세포 복합체에서 발현하는 Rpia 유전자의 종 특이적 발현)

  • Kim, Yun-Sun;Yoon, Se-Jin;Kim, Eun-Young;Lee, Kyung-Ah
    • Clinical and Experimental Reproductive Medicine
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    • v.34 no.2
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    • pp.95-106
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    • 2007
  • Objective: We previously identified differentially expressed genes (DEGs) between germinal vesicle (GV) and metaphase II (MII) mouse oocyte. The present study was accomplished as a preliminary study to elucidate the role of ribose 5-phosphate isomerase A (Rpia), the essential enzyme of the pentose phosphate pathway (PPP), in oocyte maturation. We observed expression of Rpia in the mouse and porcine oocytes. Methods: Expression pattern of the 11 MII-selective DEGs in various tissues was evaluated using RT-PCR and selected 4 genes highly expressed in the ovary. According to the oocyte-selective expression profile, we selected Rpia as a target for this study. We identified the porcine Rpia sequence using EST clustering technique, since it is not yet registered in public databases. Results: The extended porcine Rpia nucleotide sequence was submitted and registered to GenBank (accession number EF213106). We prepared primers for porcine Rpia according to this sequence. In contrast to the oocyte-specific expression in the mouse, Rpia was expressed in porcine cumulus and granulosa cells as well as in oocytes. Conclusion: This is the first report on the characterization of the Rpia gene in the mouse and porcine ovarian cells. Results of the present study suggest that the mouse and porcine COCs employ different mechanism of glucose metabolism. Therefore, the different metabolic pathways during in vitro oocyte maturation (IVM) in different species may lead different maturation rates. It is required to study further regarding the role of Rpia in glucose metabolism of oocytes and follicular cell fore exploring the regulatory mechanism of oocyte maturation as well as for finding the finest culture conditions for in vitro maturation.