• 대한물리치료과학회지
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• 제2권4호
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• pp.771-778
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• 1995

편광 근적외선 조사가 실험적 통증역치에 영향을 미치는지를 규명하기 위해서 건강한 성인의 합곡혈에 여러 단계의 출력량으로 편광 근적외선을 30초간 조사하고 시간 경과에 따라 실험적 통증역치를 측정하여 분석하였다. 실험 결과 출력량 0 %인 대조군과 10 % 및 20 %의 출력량에서는 실험적 통증역치의 유의한 변화가 없었다. 그러나 출력량 40 %군의 실험적 통증역치는 편광 근적외선 조사전 $3.85{\pm}0.51mA$에서 조사 직후 $4.77{\pm}0.87mA$, 조사 후 30분 $5.57{\pm}0.98mA$, 조사 후 1시간 $5.68{\pm}1.06mA$, 조사 후 2시간 $5.34{\pm}0.96mA$로 증가되었고, 출력량 80 %군에서도 실험적 통증역치가 조사전 $3.87{\pm}0.92mA$에서 조사 직후 $4.45{\pm}0.62mA$, 조사 후 30분 $4.91{\pm}0.51mA$, 조사 후 1시간 $4.93{\pm}0.62mA$, 조사 후 2시간 $5.55{\pm}1.11mA$로 증가하였다. 실험적 통증역치는 편광 근적외선 조사 직후부터 2시간까지 유의하게 증가하였다. 40 % 출력량과 80 % 출력량의 실험적 통증역치는 유의한 차이가 없었고, 출력량과 시간의 상호작용효과는 없었다. 건강인의 실험적 통증역치를 대상으로 한 본 연구 결과를 통증이 있는 환자에게 직접 적용하기는 어렵다고 생각하며 앞으로 환자를 대상으로한 연구가 수행되기를 기대한다.

• 대한불안의학회지
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• 제16권2호
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• pp.83-90
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• 2020

Objective : Heart rate variability (HRV) is known to reflect autonomic nervous system activity. Individuals with posttraumatic stress disorder (PTSD) are reported to have lower HRVs. We attempted to find HRV indices with head up tilt position that reflect the symptoms well in order to evaluate PTSD symptoms. Methods : Sixty-seven patients with PTSD and 72 patients without PTSD were assessed using the PTSD Checklist for DSM-5 (PCL-5), the Beck Depression Inventory, the Beck Anxiety Inventory and the Pittsburgh Sleep Quality Index. HRV was measured in the head-up tilt position. We collected data regarding heart rate (HR), standard deviation of the NN intervals (SDNN), the square root of the mean squared differences of successive NN intervals (RMSSD), log low-frequency (LNLF) and log high-frequency (LNHF). Results : The value of LNHF was different according to presence or absence of PTSD after head-up tilt position. In the findings of the association between PTSD symptoms and HRV indices as based on head-up tilt, LNHF had a significant correlation with the total score of PCL-5. Conclusion : The reduction of the high-frequency component of HRVs in the PTSD group might reflect more PTSD symptoms.

• Asian Pacific Journal of Cancer Prevention
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• 제15권22호
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• pp.9699-9706
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• 2014

Background: The predictive value of the xeroderma pigmentosum group D (XPD) Lys751Gln polymorphism regarding clinical outcomes of patients with colorectal cancer (CRC) receiving oxaliplatin-based chemotherapy has been evaluated in numerous published studies, but the results remain inconclusive. Therefore, we performed a meta-analysis to determine the precise role of the XPD Lys751Gln polymorphism in this clinical situation and optimize individual chemotherapy. Materials and Methods: A multiple search strategy was used to identify eligible studies. Pooled odds ratios (ORs), generalized odds ratio (ORG) and their 95% confidence intervals (CIs) were used to estimate the objective response, while hazard ratios (HRs) with 95%CIs were used for progression-free survival (PFS) and overall survival (OS). Results: A total of 17 studies including 2,286 patients met the inclusion criteria. Overall, the XPD 751Gln allele was associated with a non-significant reduced objective response to oxaliplatin-based chemotherapy in all patients or in the Asian and Caucasian subgroups. However, poor PFS and OS of CRC patients treated with oxaliplatin-based regimens were significantly related to the XPD 751Gln allele in the dominant model (PFS: HR=2.10, 95%CI: 1.65-2.67; OS: HR=3.18, 95%CI: 1.57-6.47). On stratified analysis by ethnicity, these relationships were more pronounced in Asians (PFS: HR=2.49, 95%CI: 1.79-3.47; OS: HR=5.25, 95%CI: 3.46-7.94) than in Caucasians (PFS: HR=1.73, 95%CI: 1.22-2.46; OS: HR=1.78, 95%CI: 1.06-2.99). Conclusions: The XPD Lys751Gln polymorphism may have prognostic value in patients with CRC undergoing oxaliplatin-based chemotherapy.

• 한국가축번식학회지
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• 제13권2호
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• pp.70-78
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• 1989

Mouse eggs recovered from oviducts at one hourly intervals between 10 and 20 hours after administration of hCG were fixed, stained and then investigated the rate of in vitro fertilization and nuclear maturation. In case of out- bred ICR mice, ovulations were occured between 11 and 13 hours after hCG injection. The stages of in vitro maturation of eggs recovered from female mice at various times after hCG injection were metaphase I, anaphase I, telophase I and metaphase II. However the majority was metaphase I(17.6 to 44.4%) and metaphase II(42.9 to 80.0%) stage. When the eggs were inseminated with epididymal spermatozoa, the fertilization rate was declined as the egg recovery time after hCG administration was delayed. That is, the proportion of eggs undergoing fertilization became higher(68.1 to 77.4%) in the eggs at 12 to 15hr after injection of hCG than those(17.5 to 56.4) at 16 to 20 hr after injection of hCG. Also, when nuclear maturation of the unfertilized eggs were observed at 8 hours after insemination, the majority was in metaphase I and metaphase II and no anaphase I and telophase I were observed.

• Nutrition Research and Practice
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• 제12권5호
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• pp.396-405
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• 2018

BACKGROUND/OBJECTIVES: This prospective study examined gender-specific associations between egg intake and the risk of developing type 2 diabetes using data from a large-scale cohort study. SUBJECTS/METHODS: A total of 7,002 Korean adults (40-69 years) without type 2 diabetes at baseline were analyzed. Dietary intake was evaluated by a food frequency questionnaire administered at baseline (2001-2002) and the second follow-up examination (2005-2006). Type 2 diabetes was diagnosed as a fasting glucose concentration ${\geq}126mg/dL$ or current use of glucose-lowering medications or insulin injection. Multivariate Cox proportional hazard models were used to calculate hazard ratios (HRs) with 95% confidence intervals (CIs) for incident type 2 diabetes according to egg consumption or cholesterol intake. RESULTS: During a 14-year follow up period, 857 subjects developed type 2 diabetes. In men, frequent egg intake (2- < 4 servings/week) was associated with a 40% lower risk of developing type 2 diabetes than infrequent egg intake (0- < 1 serving/week) (HR = 0.60, 95% CI: 0.37-0.97), whereas no association between egg intake and incidence of type 2 diabetes was observed in women (HR = 0.61, 95% CI: 0.27-1.37). There was no association between cholesterol intake and risk of incident type 2 diabetes in either men or women. CONCLUSIONS: Egg consumption was inversely related to the risk of incident type 2 diabetes in men, but not in women, suggesting gender differences in the relationship between diet and disease risk.

• 한국환경성돌연변이발암원학회지
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• 제21권2호
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• pp.122-127
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• 2001

4-tert-octylphenol (OP) is a surfactant additive widely used in the manufacture of a variety of detergents and plastic products. Also, OP is known to have estrogenic activity by interacting with development and functions of endocrine system. This study was carried out to obtain toxicokinetic parameters of OP in male Sprague-Dawley rats. Male rats were administered OP, by either single oral (gavage) applications of 50, 100 or 200 mg/kg body weight. or a single intravenous injections of 1, 5 or 10 mg/kg body weight. Blood samples taken at several time intervals after administration were obtained from the femoral artery. Analysis of blood samples for OP was performed by gas chromatography mass spectrometry (GC/MS). The detection limit of OP was 1.9 ng/$m\ell$ at SIM (selected ion monitoring) mode of GC/MS. Calibration curve for analysis of the concentrations of OP in plasma was (OP/butylphenol peak area ratio) = 0.0294 $\times$ (plasma cone.) + 0.028 ($r^2$= 0.9991). The OP plasma concentration was 3921 ng/$m\ell$ immediately after single intravenous application, decreased rapidly within 45 min, and was detectable at low concentration up to 6 hr after application. When administered orally in rats (50, 100 and 200 mg/kg), OP was detected in the blood early after gavage administration, indicating the rapid initial uptake from gastrointestinal tract, with Tmax obtained from 0.67~0.83 hr. Using the AUC (area under the curve) of plasma concentration vs. time, low oral bioavailabilities of 1.2, 5.0 and 5.3% were calculated for the 50, 100 and 200 mg/kg groups, respectively.

• Journal of Pharmaceutical Investigation
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• 제35권5호
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• pp.323-328
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• 2005

The purpose of the present study was to evaluate the bioequivalence of two torasemide tablets, Torem tablet (Roche Korea Co., Ltd., Korea, reference drug) and Boryung Torsemide tablet (Boryung Pharmaceutical Co., Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (furosemide) to human serum, serum samples were extracted using 5 mL of ethyl acetate. Compounds were analyzed by reverse-phase HPLC method with UV detection. This method showed linear response over the concentration range of 0.05 ug/mL with correlation coefficient of 0.999. The lower limit of quantitation using 0.5 mL of serum was 0.05 ug/mL which was sensitive enough for pharmacokinetic studies. Twenty-eight healthy male Korean volunteers received each medicine at the torasemide dose of 20 mg in a $2{\times}2$ crossover study. There was a one-week washout period between the doses. Serum concentrations of torasemide were monitored by an HPLC-UV for over a period of 12 hr after the administration. $AUC_{t}$(the area under the serum concentration-time curve from time zero to 12 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum serum drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the serum concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_{t}$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_{t}$ ratio and the $C_{max}$ ratio for Boryung Torsemide/Torem were log 0.97-10g 1.03 and log 0.93log 1.12, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Boryung Torsemide tablet and Torem tablet are bioequivalent.

• 한국미생물·생명공학회지
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• 제20권1호
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• pp.79-84
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• 1992

Rhodopseudomonas sphaeoides B6 세포를 agar gel 중에 고정했을 때, 수소생산을 위한 최적 agar 농도는 2(w/v)였다. B6의 2% agar gel 고정화균체(300ml gel: 2.85 mg dry cells/ml)와 비고정세포(1l culture: 0.87 mg dry cells/ml)에 있어서 초기의 최고 수소 생산활성은 각각 47.5 및 48.0ml/hr/culture로 거의 같았다. 그러나, 수소생산이 거의 정지된 배양후기에는 lactate의 제한공급( 10 mmole)에 의한 비고정세포의 활성회복은 50% 이하로 감소되었지만, 고정화세포의 활성은 거의 초기상태로 회복되었다. B6의 고정화균체를 이용하여 12시간 주기의 광조사조건으로, 매 1l의 수소생산시 마다 그에 소비된 만큼의 기질에 상당하는 9.3mmole의 DL-lactate와 1.86mmole의 L-glutamate 함유 기본배지를 주기적으로 공급한 결과, 228시간의 배양기간 동안 명암의 반복조건에도 불구하고 평균 510 ml/day/300m/ gel(2.9mg dry cells/ml)의 속도로 수소생산이 지속되었다. 이 결과는 광합성세균에 의한 수소생산에 있어서, 보다 효율적 기질공급과 가변적인 태양광 이용 조건에 대응되는 자동배양 시스템의 구성 가능성을 시사한다.

• 한국임상약학회지
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• 제10권1호
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• pp.25-29
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• 2000

Bioequivalence of cisapride-containing $Cisaplus^{(R)}$ tablets (Daewoong Co.) to reference $Prepulsid^{(R)}$ tablets (Janssen Co.) was evaluated according to the guidelines of Korea Food and Drug Administration (KFDA). Sixteen healthy volunteers were divided randomly into two groups and administered orally at a cisapride dose of 10 mg in a $2\times2$ crossover design. There was a 1-week washout period between the treatments. Blood samples were taken at predetermined time intervals for 48 hr and the plasma cisapride concentrations were determined by an HPLC with UV detector. The area under the plasma drug concentration-time curve (AUC) was caltulated from time zero to the last sampling time by a linear trapezoidal method. The maximum observed plasma drug concentration ($C_{max}$) and the time to $C_{max}\;(T_{max})$ were estimated directly from the drug concentration-time data. Analysis of variance (ANOVA) showed that the apparent differences for AUC, $C_{max}\;and\;T_{max}$ were $-7.52\%,\;-8.91\%\;and\;-15.55\%$, respectively. The minimum detectable differences for AUC, $C_{max}\;and\;T_{max}$ between formulations were $14.52\%,\;11.57\%\;and\;28.00\%$ respectively, at $\alpha=0.05\;and\;1-\beta=0.8\;levels.\;The\;90\%$ confidence intervals for AUC, $C_{max}\;and\;T_{max}\;were\;-16.00\sim0.97\%,\;-15.67\sim-2.15\%\;and\;-31.88\%\sim0.84\%$, respectively. These results satisfy the bioequivalence criteria of KFDA guidelines, indicating that the two formulations of cisapride are bioequivalent.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2002년도 학술발표회 논문집(II)
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• pp.1187-1194
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• 2002

In this study, we have simulated regional climate over East Asia using dynamical downscaling For dynamic downscaling experiments for regional climate simulation, MM5method. with 27 km horizontal resolution and 18 layers of sigma-coordinate in vertical is nested within global-scale NCEP reanalysis data with 2.5。${\times}$2.5。 resolution in longitude and latitude. In regional simulation, January and July, 1979 monthly mean features have been obtained by both continuous integration and daily restart integration driven by updating the lateral boundary forcing at 6-hr intervals from the NCEP reanalysis data using a nudging scheme with the updating design of initial and boundary conditions in both continuous and restart integrations. In result, we may successfully generated regional detail features which might be forced by topography, lake, coastlines and land use distribution from a regional climate. There is no significant difference in monthly mean features either integrate continuously or integrate with daily restart. For climatologically long integration, the initial condition may not be significantly important. Accordingly, MM5 can be integrated for a long period without restart frequently, if a proper lateral boundary forcing is given.