• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년도 제30회 춘계학술대회논문집
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• pp.159-162
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• 2008

발사체의 상단에 사용되는 여러 가지 싸이클의 특성을 조사한 뒤, 그 중 가스발생기 후연소 싸이클 엔진의 특성을 살펴보았다. 발사체 상단에 사용되는 엔진은 추진제와 싸이클의 특성상 연소압-확장비 다이어그램에서 크게 3그룹으로 나뉘어 진다. 영역 II에 위치한 케로신 엔진은 모두 가스발생기 후연소 싸이클 엔진으로서 높은 압력과 복잡한 구조를 하고 있다. 이 싸이클은 그 특성상 2개 이상의 펌프를 사용한다. 즉, 연료라인을 둘로 분기하여 보다 높은 압력이 요구되는 가스발생기 라인에는 2차 펌프를 두어 좀 더 가압을 하여 보다 효율적인 파워사용이 가능하다. 기본적으로 모든 산화제는 가스발생기를 지나 연소기로 향하기 때문에 2차 펌프의 필요성이 줄어들지만 여러가지 이유로 주펌프 이전에 부스터 펌프를 두어 주산화제 펌프의 부담을 덜어주는 경우가 많다. 폐쇄형 엔진은 그 특성상 엔진 비추력 효율이 개방형 엔진보다 상대적으로 높기 때문에 상단엔진에 적합하다.

• 설비공학논문집
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• 제21권6호
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• pp.333-339
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• 2009

The objective of this study is to predict optimal intermediate pressure of a 2-stage compression heat pump system using river water. To determine the maximum performance of the 2-stage compression heat pump system, the experimental evaluations on the 2-stage compression cycle were carried out under various operating conditions. Electronic expansion valves were applied to control intermediate pressure and superheat. Based on the experimental data, an empirical correlation for predicting optimal intermediate pressure which considering cycle operating parameters was developed. The present correlation was verified by comparing the predicted data with the measured data. The predictions showed a good agreement with the measured data within a relative deviation of ${\pm}4%$ at various operating conditions.

• Reproductive and Developmental Biology
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• 제35권4호
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• pp.463-468
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• 2011

This study investigated the changes of plasminogen activators (PAs) activity, expression and localization of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA) during the estrous cycle in pigs. Estrous cycle was sorted into three group by pre-ovulation (Pre-Ov), post-ovulation (Post-Ov) and early to mid-luteal stages (Early to mid-L). Analysis for immunohistochemistry was confirmed by location of tPA and uPA. Porcine uterus tissue was cut into $1{\times}1$ cm squares, and were incubated in DMEM/F-12 medium for 1 h at $38^{\circ}C$, 5% $CO_2$ for measurement of PA activity. Western blotting was implemented for measurement of PA quantity. In results, the blood vessels and secretory glands were increased in Post-Ov stage than Pre-Ov and Early to mid-L stages. The tPA and uPA was located mainly within lumen of blood vessels and secretory glands. The PA activity in Post-Ov ($0.99{\pm}0.03$) stage were significantly (p<0.01) higher than Pre-Ov stage ($0.51{\pm}0.03$) and Early to mid-L stage ($0.21{\pm}0.04$). Expression of PAs were significantly (p<0.05) higher in Early to mid-L stage than other stages. These results indicate that PAs activity and expression may change in uterus tissue during the estrous cycle in pigs.

• 한국수산과학회지
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• 제34권6호
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• pp.656-660
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• 2001

우럭, Mya arenaria은 자웅이체로서 난생이다. 생식소는 소화맹낭과 근육층 사이에 분포하고 있다. 난소와 정소는 작은 소낭들로 구성되어 있고, 성숙하면 비후된다. 이들 소관의 내강상피가 생식상피 기능을 하고 있다. 난소 및 정소의 발달에는 간충조직과 과렵세포들이 영양세포로 관여하고 있으며, 방란 방정을 마친 생식소는 완전 퇴화하고, 짧은 회복기를 거쳐 생식소낭의 형태를 갖추며 새로 분화 재배치된 망 상결체조직에서 생식소가 신생되어 기능을 발휘한다. 비만도는 99년 4월 (0.054)에 최고값을 나타내었으며, 방출기가 종료된 직후 1998년 10월에 최저값 (0.030)을 보였다. 완숙난은 $60{\mu}m$ 전후로 하나의 큰 인을 가진다. 암수의 성비는 0.89:1로 수컷이 약간 높게 나타났다. 생식주기는 초기활성기 (2월$\~$3월), 후기활성기 (4월$\~$8월), 완숙기 (9월),부분방출 및 방출기 (9월$\~$10월) 그리고 비활성기 (11월$\~$1월) 등의 연속적인 주기로 구분할 수가 있다. 주산란기는 $9\~10$월로 추계산란종이다.

• 한국가축번식학회지
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• 제20권3호
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• pp.307-313
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• 1996

This study was conducted to investigate the effect of S-phase synthronized nuclear transfer on the development of nuclear transplant bovine embryos. A blastomere derived from the 16~32 cell stage bovine embryos was transferred into an enucleated metaphase II(MII) oocytes or activated S-phase eggs. From the MII-phase and S-phase nuclear transfer, 6.3%(4/63) and 13.8%(9/65) of nuclear transplant embryos developed to the blastocyst stage, respectively. In the S-phase nuclear transfer, maximal proportion of embryos developed to the blastocyst stage(16.6%) was obtained after the recipient cell was activated 8 h prior to receving a donor nucleus. MII-phase nuclear transplant embryos showed the PCC state of their nuclear at 1.5~2 h after fusion, whereas, S-phase nuclear transplant embryos did not undergo PCC. The result of this study suggests that if blastomeres of unknown cell-cycle-stage are used, S-phase nuclear transplantation through the activation of enucleated oocytes prior to fusion enhances development of nuclear transplant embryos. This result also suggests that the interval time from oocyte activation to cell fusion may affect the development of nuclear transplant embryos.

• 한국패류학회지
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• 제23권2호
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• pp.165-172
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• 2007

우리나라 남해안 강진만에서 2006년 5월부터 2007년 4월까지 채집된 개조개의 생식소 발달과 생식주기를 조직학적으로 조사하였다. 개조개는 자웅이체로 난소는 수많은 난소소낭 (oogenic follicle) 으로 이루어져 있으며, 정소는 다수의 정소 소낭 (acinus) 으로 구성되어 있다. 생식소지수는 4월에 최고값을 나타내었고, 그 후 감소되어 7월에 최저값를 나타내었다. 비만도지수는 6월에 최고값을 보였고, 그 후 감소되어 11월에 최저값을 보였다. 개조개의 생식주기는 회복 및 휴지기 (6-12월), 초기활성기 (1-4월), 후기활성기 (2-7월), 완숙기 (3-10월), 방출기 (4-10월)로 구분되었다. 강진만에서 개조개의 주산란기는 7-9월이었다. 암, 수 성비는 1:1로 유의한 차를 보이지 않았다.

• Clinical and Experimental Reproductive Medicine
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• 제49권3호
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• pp.210-214
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• 2022

Objective: Platelet-rich plasma (PRP) therapy has received a considerable attention as an adjunct to fertility treatments, especially in women with very low ovarian reserve and premature ovarian insufficiency. Although recent studies have demonstrated that PRP led to improvements in folliculogenesis and biomarkers of ovarian reserve, the effect of intraovarian PRP administration on embryo genetics has not been studied. Methods: We report a pilot study of patients who had preimplantation genetic testing for aneuploidy (PGT-A) before and then within 3 months following PRP administration. Twelve infertile women with at least one prior failed in vitro fertilization (IVF) cycle underwent ovarian stimulation (cycle 1) with a gentle stimulation protocol and PGT-A performed at the blastocyst stage. Following cycle 1, autologous intraovarian PRP administration was performed. Within 3 months following PRP administration, the patients underwent cycle 2 and produced blastocysts for PGT-A. The percentage of euploid embryos between both cycles was compared. Results: The mean age of all participants was 40.08±1.46 years, and their mean body mass index was 26.18±1.18 kg/m². The number of good-quality embryos formed at the blastocyst stage was similar between cycle 1 and cycle 2 (3.08±0.88 vs. 2.17±0.49, respectively; p=0.11). Among all patients in cycle 1, 3 of 37 embryos were euploid (8.11%) while in cycle 2, 11 out of 28 embryos were euploid (39.28%, p=0.002). Three clinical pregnancies were noted among this patient group. Conclusion: This novel study is the first to present an improvement in the embryo euploidy rate following intraovarian PRP application in infertile women with prior failed IVF cycles. The growth factors present in PRP may exhibit a local paracrine effect that could improve meiotic aberrations in human oocytes and thus improve euploidy rates. Whether PRP improves live birth rates and lowers miscarriage rates remains to be determined in large trials.

• Fisheries and Aquatic Sciences
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• 제1권1호
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• pp.82-93
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• 1998

Monthly changes of the gonad follicle index (GFI), reproductive cycle, egg-diameter composition, first sexual maturity of the Pacific oyster, Crassostrea gigas, were studied based on the samples which have been collected from the intertidal zone of Poryong west coast of Korea, from January to December, 1996. C. gigas, is dioecious, while a few individuals are alternatively hermaphroditic. Monthly variation of gonad follicle index (GFI) used for determination of spawning period, coincided with the reproductive cycle. GFI increased from April when seawater temperatures gradually increased and reached the maximum in May. And then, GFI sharply decreased from June to September due to spawning. Reproductive cycle of this species can be divided into five successive stages: in females, early active stage (March to April), late active stage (April to May), ripe stage (May to August), partially spawned stage (June to September) and spent/inactive stage (September to February); in males, early active stage (February to March), late active stage (April to May), ripe stage (May to September), partially spawned stage (June to September) and spent/ inactive stage (September to February). The diameter of fully mature eggs are approximately 50um. Spawning occurred from June to September, and two spawning peaks were observed in June and August when the seawater temperature was above $20^{\circ}C$. Percentages of the first sexual maturity of males of 20.1-25.0 mm in shell height were over $50\%$, while those of females of 25.1-30.0 mm in shell height were over $50\%$. All the males of > 30.1 mm and all the females of ^gt; 35.1 mm completed their first sexual maturity. The results suggest that C. gigas has a protandry phenomenon. Sex ratios of 919 oysters observed were 453 females $(49.29\%)$, 429 males $(46.68\%)$, 16 hermaphrodites $(1.74\%)$, and 21 indeterminate individuals $(2.29\%)$. In age class I, sex ratio of males were $64.00\%$, thus, a higher percentage than that of females. It was noted that $64.00\%$ of the young males (age class I) were more functional than females in age class I, but 2-3 year-old oysters showed higher percentage of females. Percentages of hemaphrodites in 2-3 year classes were relatively higher than those in other year classes. Histological pattern of hermaphrodites can be divided into two types: Type I (hermaphrodite having a number of newly formed developing oocytes on the oogenic tissues within a degenerating spermatogenic follicle after discharge of numerous spermatozoa) and Type II (hermaphrodite having two separate follicles in the same gonad).

• 한국의류학회지
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• 제25권2호
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• pp.287-292
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• 2001

This study was conducted to investigate preferred clothing selected by females for thermal comfort in follicular phase (FP) and luteal phase (LP) of the menstrual cycle. Eight healthy college students volunteered as subjects. They stayed for 60 minutes at $25^{\circ}C$, 55%RH (stage 1) for baseline followed by a 60 minute stay at 2$0^{\circ}C$, 55%RH (stage 2) in a climatic chamber. Obtained results were analyzed using paired t-test and repeated measures of ANOVA. Arm and hand skin temperatures were maintained higher in FP than in LP, while mean skin temperature did not show any significant difference between FP and LP. The subjects selected additional clothing faster in FP than in LP. Six of the subjects wore heavier clothing in FP than in LP, while two of them wore heavier in LP than in FP.

• Reproductive and Developmental Biology
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• 제35권2호
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• pp.175-183
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• 2011

The present study compared the developmental potential, telomerase activity and transcript levels of X-linked genes (ANT3, HPRT, MeCP2, RPS4X, XIAP, XIST and ZFX) in the bovine somatic cell nuclear transfer (SCNT) embryos derived from different age and cell cycle of female donor nucleus. In experiment 1, the fusion rate, cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cells was slightly increased in embryos cloned with fetal fibroblasts compared to those with adult fibroblasts, but there was no significantly (p<0.05) differences. Telomerase activity was also similar in blastocysts cloned with fetal and adult fibroblasts. Up-regulated RPS4X and down-regulated MeCP2, XIAP, and XIST transcript level were observed in blastocysts cloned with adult fibroblasts, compared to those with fetal fibroblasts. In experiment 2, the fusion rate, cleavage rate to 2-cell stage, developmental rate to blastocyst stage, and the mean number of total and ICM cells was significantly (p<0.05) increased in embryos cloned with fetal fibroblasts at early G1 phase of the cell cycle, compared to those of fetal fibroblasts at late G1 phase. DNMT1 transcript was observed to significantly (p<0.05) increased in the fetal fibroblasts at 3 hrs after trypsin treatment of confluent culture. Further, level of telomerase activity and transcribed X-linked genes was also significantly (p<0.05) higher in the early G1 SCNT blastocysts than those of late G1. The results imply that fetal fibroblasts at early G1 phase induces the enhanced developmental potential and up-regulated telomerase activity and X-linked gene, but aberrant transcript pattern of X-linked genes may be displayed in the SCNT embryos.