• 대한기계학회논문집
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• 제19권2호
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• pp.591-596
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• 1995

In order to investigate the reduction of NO emissions, natural gas was fueled for two-stage combustion apparatus. NO and CO emissions were described by five variables: total air ratio, primary air ratio, secondary air injection position, secondary air injection velocity, and swirl ratio. It was mainly observed that, as the primary air ratios of 0 and 0.4 NO emission decreased with increasing the secondary air injection position and secondary air injection velocity. The effect of weak swirl on NO emission was found to be insignificant.

• 한국정밀공학회지
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• 제13권9호
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• pp.130-137
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• 1996

In the present paper the effect of various process conditions on the final weight of injection molded rectangular plates has been investigated in detail. The main parameters involved in the simulations were melt temperature, mold temperature, injection speed and packing pressure. The dimensions of the plate used were 100mm long, 2mm of width and polystyrene was used as a molding material. The shear viscosity of the polymeric material was treated as a function of shear rate, temperature and pressure through the whole processes including packing and cooling stages. By increasing a packing pressure the final weight of sample increased linearly. Furthermore, as the melt temperature, the mold temperature and the injection speed increased, the final weight of the injection molded plate decreased within the molding window.

• 한국분무공학회지
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• 제5권4호
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• pp.33-39
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• 2000

The behavior of the 2-stage spray was studied by using the schlieren method with the high pressure common-rail injection system. The spray injected 2 times with the interval of $0.3ms{\sim}1.5ms$ between the 1st and the 2nd spray in a modeled combustion chamber of constant volume bomb. In this case, the quantity of injected fuel of 1st and 2nd also changed. The schlieren photograph shows that the 2nd spray goes further away than the 1st spray when the quantity of the 1st spray is less than that of the 2nd spray. The dispersion of the vapour to the combustion chamber is not affect in a 10% of 1st spray quantity. When the 1st spray quantity is more than the 2nd spray, the vapour scattering of spray is good.

• 한국수정란이식학회지
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• 제9권2호
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• pp.153-160
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• 1994

This study evaluated the influence of cell stage of donor nucleus on nuclear injection, electrofusion and in vitro development in the rabbit to improve the efficiency of nuclear transplantation in the rabbit. The embryos of 8-, 16- and 32-cell stage were collected from the mated does by flushing viducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FGS) at 44, 54 and 60 hours after hCG injection. The blastorneres separated from these embryos were used as donor nucleus. The ovulated oocytes collected at 14 hours after hCG injection were used as recipient cytoplasm following removing the nucleus and the first polar body. The separated blastomeres were injected into the enucleated oocytes by micromanipulation and were electrofused in 0.28 M mannitol solution at 1.5 kV /cm, 60 $\mu$sec for three times. The fused oocytes were cocultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FGS for 72~120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. The cultured nuclear transplant embryos were stained with Hoechst 33342 solution and the number of cells were counted by fluorescence microscopy. The successful injection rate of 8-, 16- and 32-cell-stageblastomeres into enucleated oocytes was 86.7, 91.0 and 93.9%, respectively. The electrofusion rate of 8-, 16- and 32-cell-stage blastomeres with enucleated oocytes was 93.3,89.3 and 79.0%, respectively. Development of blastomeres to blastocyst was similar with 8-,16- and 32-cell-stage donor nuclei(26.2, 25.8 and 26.6%, respectively, P<0.05). The mean number of cell cycle per day during in vitro culture in nuclear transplant embryos which received 8-, 16- and 32-cell- stage nuclei was 1.87, 1.81 and 1.43, respectively.

• 한국자동차공학회논문집
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• 제14권4호
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• pp.26-31
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• 2006

An experimental investigation of an early injection strategy was conducted on a small single cylinder common-rail DI diesel engine to reduce the oxides of nitrogen($NO_x$) emission. The main objectives of this study were to investigate the emissions, performance and combustion characteristics in a diesel engine with early and two-stage injections. The two- stage injection was conducted to reduce the wall-wetting of early injected fuels on the cylinder wall or to promote the ignition of premixed charge. The engine test was performed at conditions of 1500rpm, injection timing ranging from TDC to BTDC $80^{\circ}$. The experimental results show that $NO_x$ emissions were decreased in both cases of early injection and two stage injection compared to the conventional diesel combustion by the near TDC injection. However, soot and products of incomplete products (i.e. HC and CO) are slightly increased. Also, the second injection near TDC promoted the ignition of premixed fuel, therefore, IMEP was increased.

• 설비공학논문집
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• 제21권8호
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• pp.425-432
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• 2009

In this study, the improvement of cooling capacity by applying gas injection technique in a two-stage heat pump using R410A was experimentally investigated. A twin rotary type compressor with gas injection was applied to the heat pump system. The optimum refrigerant charge for the injection and the non-injection cycles was selected to achieve the maximum COP at the cooling standard condition. The injection cycle showed less optimum refrigerant charge than that of the non-injection cycle. The cooling performances of the injection and the non-injection cycles were measured and compared by varying compressor frequency from 40 to 90 Hz. The cooling capacity of the gas injection cycle was 1.6% -11.3% higher than that of the non-injection cycle. The COP of the gas injection cycle was 13.7% to 28.9% higher than that of the non-injection cycle at the same cooling capacity. The heat pump system showed stable operation after 30% of the injection valve opening.

• 한국자동차공학회논문집
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• 제12권2호
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• pp.24-31
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• 2004

A cylindrical constant volume combustion bomb is used to investigate the combustion characteristics and to analyzer the heat quantity of inhomogeneous charge methane-air mixture. To analyze the heat quantity, some definitions including the CHR ratio, the UHC ratio and the HL ratio are needed and are calculated. It is shown that the effect of stratification is not significant in case of the overall excess air ratio of 1.1, mainly due to the higher heat loss and lower thermal efficiency compared to those of homogeneous condition. In the case of the overall excess air ratio of 1.4, as the initial charge pressure decreases, the CHR ratio has been decreased while the HL ratio has been increased, Generally, as the initial charge pressure increases, the amount of injection mixture has been decreased and has resulted in lower mean velocity and turbulence intensity for injection mixture. Also, the injected mixture is too rich to result in mixing deficiency in combustion chamber. From these results, it could be possible to acquire the improvement of thermal efficiency and the reduction of heat loss simultaneously through the 2-stage injection in CNG direct injection engine.

• 대한기계학회논문집B
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• 제37권7호
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• pp.675-682
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• 2013

전단 동축 분사기의 Inner-stage와 Outer-stage의 기체 분사 비율 변화에 따른 축방향 유동 분포 특성과 분무 분열 특성을 실험적으로 연구였다. 무차원 측정 거리를 Z/d=100까지 변화시킴에 따라 운동량 교환, 공기역학적 항력, 점성 혼합의 영향으로 완전 발달된 유동의 형태를 나타내었다. Inner-stage의 기체분사와 Outer-stage의 기체 분사의 영향은 Z/d=5 이내의 영역에서 간섭받지 않고 분무 초기에 Inner-stage에서 분사된 기체 전단력에 의해 분열됨을 파악할 수 있었으며, Z/d=10 이상의 영역에서 완전 발달된 유동으로 변화하며, 유동의 혼합이 진행됨을 관찰 할 수 있었다. Inner-stage의 운동량 플럭스 비 0.84 이내에서 Outer-stage의 운동량 플럭스 비가 증가함에 따라 SMD가 감소하는 경향을 나타내었으며, Inner-stage의 운동량 플럭스 비가 1.38 이상의 조건에서 SMD의 분포가 유사하게 나타나는 경향을 관찰할 수 있었다.

• Reproductive and Developmental Biology
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• 제29권4호
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• pp.229-234
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• 2005

Methods for activation of reconstructed oocytes were examined for the production of nuclear transfer (NT) rat embryos using fetal neural stem cells as donor. Neural stem cells were isolated from Day 14.5 rat fetuses, and the oocytes for recipient cytoplasm were recovered from 4-week old Sprague Dawley rats. After enucleation and nuclear injection, the reconstructed oocytes were immediately exposed to activation medium consisting of 10 mM $SrCl_2$ for 4 h (immediate activation after injection; IAI), or cultured in vitro for $2\~3$ h before activation treatment (injection before activation; IBA). Pre-activated oocytes were also used for NT to test reprogramming potential of artificially activated oocytes. The oocytes were grouped as IIA (immediate injection after activation) and ABI (activation $2\~3$ h before injection). Following NT, the oocytes were cultured in vitro. Development of the NT embryos was monitored at 44 and 119 h after activation. The embryos in groups IAI, mA, and IIA were cleaved to the 2-cell stage at the rates of $36.6\%\;(15/41),\;39.5\%\;(17/43)\;and\;46.3\%$ (25/54), respectively. However, in the ABI group, only one embryo ($1.8\%$, 1/55) was cleaved after activation. After in vitro culture, two NT embryos from IAI group had developed to the morula stage $(4.9\%\cdot2/41)$. However, no morula or blastocyst was obtained in the other groups. These results suggest that immediate activation after injection (IAI) method may be used for the production of rat somatic cell NT embryos.

• 한국자동차공학회논문집
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• 제2권6호
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• pp.110-116
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• 1994

In this research, the tensile strength of molded parts and pressure distribution were analyzed to study the cavity filling stage and packing stage in injection molding. The measurement of cavity pressure was obtained by a data acquisition system with the installation of transducers in the cavity. Molded parts were tested by a universal testing machine to obtain the tensile strength. For the experimental work, the tensile strength of molded parts increased with longer packing time and exact freezing time of the gate was obtained by a cavity pressure curve. In addition, the effect of packing did not occur and tensile strength was almost constant after early 1.5 sec of the freezing time of gate. Density tended to be higher about 0.2% due to a larger degree of mold temperature and melt temperature. Also, changing pressure in the cavity was effectively sensed. Thereafter, the possibility of the development of pattern recognition expert system was confirmed on the basis of the experimental results.