• Title/Summary/Keyword: 2-Loop Structure

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A systems thinking approach to explore the structure of urban walking and health promotion in Seoul (서울시민의 보행과 건강증진에 관한 시스템 사고 기반의 구조 탐색)

  • Kim, Dong Ha;Chung, Chang-Kwon;Lee, Jihyun;Kim, Kwang Kee;JeKarl, Jung;Yoo, Seunghyun
    • Korean Journal of Health Education and Promotion
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    • v.35 no.5
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    • pp.1-16
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    • 2018
  • Objectives: This study aimed to examine systems behavior of urban walking by analyzing a dynamic structure in Seoul, South Korea. Methods: As a systems thinking approach to urban walking and health promotion, we developed a Casual Loop Diagram based on literature review and expert consultation. The reviewed literature included: 1) qualitative studies that explores the experiences of urban walkers in Seoul; 2) a systematic review study on the built environmental factors related to walking; 3) policy research reports related to urban walking in Seoul. Results: The feedback structure for urban walking was related to the three urban environments (safety & walking environment, socioeconomic environment, and public transportation environment), and was characterized by a trade-off consisting of eight reinforcing loops and four balancing loops. Conclusions: The policies for a walkable city require multi-sectoral cooperation in order to change the causal loop structure related to the decline of walking. Therefore, it is necessary to establish legal and institutional conditions so that multi-sectoral and multidisciplinary approaches are possible.

Crystal Structure of Cytochrome cL from the Aquatic Methylotrophic Bacterium Methylophaga aminisulfidivorans MPT

  • Ghosh, Suparna;Dhanasingh, Immanuel;Ryu, Jaewon;Kim, Si Wouk;Lee, Sung Haeng
    • Journal of Microbiology and Biotechnology
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    • v.30 no.8
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    • pp.1261-1271
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    • 2020
  • Cytochrome cL (CytcL) is an essential protein in the process of methanol oxidation in methylotrophs. It receives an electron from the pyrroloquinoline quinone (PQQ) cofactor of methanol dehydrogenase (MDH) to produce formaldehyde. The direct electron transfer mechanism between CytcL and MDH remains unknown due to the lack of structural information. To help gain a better understanding of the mechanism, we determined the first crystal structure of heme c containing CytcL from the aquatic methylotrophic bacterium Methylophaga aminisulfidivorans MPT at 2.13 Å resolution. The crystal structure of Ma-CytcL revealed its unique features compared to those of the terrestrial homologues. Apart from Fe in heme, three additional metal ion binding sites for Na+, Ca+, and Fe2+ were found, wherein the ions mostly formed coordination bonds with the amino acid residues on the loop (G93-Y111) that interacts with heme. Therefore, these ions seemed to enhance the stability of heme insertion by increasing the loop's steadiness. The basic N-terminal end, together with helix α4 and loop (G126 to Y136), contributed positive charge to the region. In contrast, the acidic C-terminal end provided a negatively charged surface, yielding several electrostatic contact points with partner proteins for electron transfer. These exceptional features of Ma-CytcL, along with the structural information of MDH, led us to hypothesize the need for an adapter protein bridging MDH to CytcL within appropriate proximity for electron transfer. With this knowledge in mind, the methanol oxidation complex reconstitution in vitro could be utilized to produce metabolic intermediates at the industry level.

Repetitive operation characteristics of 500 Hz class excimer laser (500 Hz급 엑시머레이저의 반복동작특성)

  • 박홍진;이주희
    • Korean Journal of Optics and Photonics
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    • v.7 no.3
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    • pp.232-237
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    • 1996
  • A 500 Hz repetition rate excimer laser was developed as light source for pollution lidar. In this paper, the high repetitive output characteristics, the gas flow loop structure, and CR(clearing ratio) characteristics were investigated. Our laser system was constructed compact structure with a streamline gas flow loop and UV preionization. The real gas volume of laser is 10 liter. At 500 Hz repetitive operation, we have obtained average power of 53 watt with KrF laser gas. The variation of laser output, CR, and active volume are $\pm$6.7%, 2.3, and 2.0(H)$\times$1.2(W)$\times$56(L)=134 ㎤, respectively. Laser output power is declined to half at 3$\times$$10^6$ shots.

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Flow-Induced Vibration Signal Analysis of the FIV Test Loop (FIV 시험루프의 유동기인 진동 신호분석)

  • Lee, Kang-Hee;Kang, Heung-Soek;Yoon, Kyung-Ho;Song, Kee-Nam
    • Proceedings of the KSME Conference
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    • 2004.11a
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    • pp.601-606
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    • 2004
  • Vibration spectrums of the test loop according to flow conditions were analyzed in order to identify the sources of vibration at peak frequencies. While a flow condition of the sweep test was changed by varying pump rotational speed from 450 rpm to 1500 rpm by the step 150 rpm, midspan acceleration of the test section in width-direction and dynamic pressure perturbation in the test section were measured. Other sources of vibration due to the flow structure interactions, such as acoustic resonance, blade pulsing frequency and bellows wrinkles, were investigated. Pressure perturbation in the section and acoustic resonance due to branch pipe give major effects to the vibration of the test section in high frequency range of 1.5 kHz to 2.8 kHz.

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Design of a Robust Controller Using Disturbance Rejection Controller (외란 제거 제어기를 이용한 강인 제어기의 설계)

  • Yeo, Hee-Joo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.2
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    • pp.137-144
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    • 2006
  • This paper proposes the design of a robust controller based on disturbance observer which is strong to variation of system parameters, uncertainty of models or external disturbance. The controller consists of a mode based compensator and a feedback controller based on two-loop structure. The compensator in the internal-loop removes internal and external disturbances and a feedback controller in the external loop achieves performance along with given specifications. As a result, it shows that the proposed robust controller can stabilize a system against disturbance and improve controlling performance.

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Characteristics of the Flux-lock Type Superconducting Fault Current Limiter According to the Iron Core Conditions (자속구속형 초전도 전류제한기의 철심조건에 따른 특성)

  • Nam, Gueng-Hyun;Lee, Na-Young;Choi, Hyo-Sang;Cho, Guem-Bae
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.20 no.7
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    • pp.38-45
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    • 2006
  • The superconducting fault current limiters(SFCLs) provide the effect such as enhancement in power system reliability due to limiting the fault current within a few miliseconds. Among various SFCLs we have developed a flux-lock type SFCL and exploited a special design to effectively reduce the fault current according to properly adjustable magnetic field after the short-circuit test. This SFCL consists of two copper coils wound in parallel on the same iron core and a component using the YBCO thin film connected in series to the secondary copper coil. Meanwhile, operating characteristics can be controlled by adjusting the inductances and the winding directions of the coils. To analyze the operational characteristics, we compared closed-loop with open-loop iron core. When the applied voltage was 200[Vrms] in the additive polarity winding, the peak values of the line current the increased up to 30.71[A] in the closed-loop and 32.01[A] in the open-loop iron core, respectively. On the other hand, in the voltages generated at current limiting elements were 220.14[V] in the closed-loop and 142.73[V] in the opal-loop iron core during first-half cycle after fault instant under the same conditions. We confirmed that the open-loop iron core had lower power burden than in the closed-loop iron core. Consequently, we found that the structure of iron core enabled the flux-lock type SFCL at power system to have the flexibility.

Application of multi dimensional NMR experiments to VBS RNAs of Yeast Saccaromyces cerevisiae virus

  • Chaejoon Cheong;Cheong, Hae-Kap;Yoo, Jun-Seok
    • Journal of the Korean Magnetic Resonance Society
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    • v.5 no.1
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    • pp.29-36
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    • 2001
  • The structures of two VBS (viral binding site) RNAs, SL1 and SL2, of Yeast Saccharomyces cerevisiae vims have been studied by 2D and 3D NMR experiments. VBSs play a crucial role in viral particle binding to the plus strand and packaging of the RNA. The secondary structures of the two VBS RNAs share a common feature of the stem-internal loop-stem-hairpin loop structure although the size of the internal loops of SL1 and SL2 differs. 2D experiments were sufficient for fill assignments of SL1. However, isotope labeling of the sample and multidimensional experiments were required for 28-nucleotide-long SL2 due to the spectral overlap. Several 3D HCCH experiments have accomplished full assignment of SL2 RNA.

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Study on Backfire for a Two-Stroke Hydrogen Fueled Free-Piston Engine with Loop Scavenging (루프소기방식을 갖는 2행정 프리피스톤 수소기관의 역화에 관한 연구)

  • Cho, Kwan-Yeon;Byun, Chang-Hee;Back, Dae-Ha;Lee, Jong-Tae
    • Journal of Hydrogen and New Energy
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    • v.21 no.6
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    • pp.487-492
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    • 2010
  • For developing a two-stroke free-piston hydrogen engine with high efficiency and low emission, determination of the scavenging type is one of the most important factor. In this research, backfire characteristics for loop scavenging were analyzed with the number of piston crevice volume and piston expansion speed. Rapid Compression Expansion Machine, RCEM was used for combustion research of the free piston $H_2$ engine in the experiment. As the results, it was shown that although backfire occurring in a loop scavenging type can be partially controled by a complete exhaust of burned gas, possibility of backfire basically exist due to the structure which piston crevice volumes contact with fresh mixture in a scavenging port. However, a loop scavenging may be considered as combustion chamber of a free piston $H_2$ engine from the point of view that backfire does not occur nearby lean equivalence ratio obtained high thermal efficiency. It was also analyzed that an advances of backfire occurrence timing with increase of the fuel-air equivalence ratio were due to promotion of flame propagation into piston crevice volumes by decrease of the quenching distance.

A Reconfigurable Antenna for Quad-Band Mobile Handset Application (4중 대역을 포함하는 휴대폰용 재구성 안테나)

  • Park, Young-Keun;Sung, Young-Je
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.5
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    • pp.570-582
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    • 2012
  • In the communication, a reconfigurable antenna using two PIN diodes is presented for quad-band(GSM900/GSM1800/GSM1900/UMTS) mobile handset applications. The proposed antenna has a size $45{\times}11{\times}6mm^3$. By independently adjusting the on/off states of two PIN diodes located on the radiating element, the proposed structure can be operated in the PIFA and loop mode, respectively. The PIN diodes are replaced by conducting tape in order to verify the concept. In regards to the fabricated reconfigurable antenna, when operating in the PIFA mode, the measured results show that the 7 dB bandwidth is 8.62 %. which covers the GSM900(880~960 MHz) band. When operating in the loop mode, the measured results show that the 7 dB bandwidth is 26.36 %, which covers the GSM1800(1,710~1,880 MHz), GSM1900(1,850~1,990 MHz), and UMTS(1,920~2,170 MHz) bands, respectively.

Structural Studies on IRES 4-2 Domain of Foot-and-mouth Disease Virus

  • Kim, Young-Mee;Yoo, Jun-Seok;Cheong, Hae-Kap;Lee, Chul-Hyun;Cheong, Chae-Joon
    • Journal of the Korean Magnetic Resonance Society
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    • v.7 no.2
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    • pp.89-97
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    • 2003
  • Foot-and-mouth disease virus (FMDV) belongs to the aphthovirus genus within the picornavirus which has a single copy of a positive sense RNA. The translation initiation process of FMDV occurs by a cap-independent mechanism directed by a highly structured element (∼435 nt) termed an internal ribosome entry site (IRES). We have designed and prepared FMDV 4-2 RNA (28nt) by in vitro transcription. The 2D NMR data revealed that FMDV 4-2 IRES domain RNA has a flexible loop and bulge conformation. In further study, we need to make an isotope labeled RNA sample and conduct 3D NMR experiments to completely determine the 3D structure. This study may establish a new drug design strategy to treat foot-and mouth disease.

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