• Clinical and Experimental Reproductive Medicine
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• v.28 no.3
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• pp.191-198
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• 2001

Objective : This study was conducted to examine the effect of vitrification on the survival and in vitro development of mice 1-cell zygotes. Method: Effects of exposure to vitrification solution and vitrification, with different concentrations of the cryoprotectant solution, were examined. The 1-cell zygotes were also subjected to a slow freezing-thawing method to compare with vitrification method. Solution composed of ethylene glycol (6.0 M, 5.0 M, 4.0 M) and sucrose (1.0 M) were used as cryopropectant. The experiments employed the method loading the embryos on electron microscope grids. Results: I. The effects of exposure in vitrification solution. 1-cell zygotes were non-toxic at all concentrations of the vitrification solution showing the survival rate between 88.1% and 97.5%. Development into 2-cell was more successful in the higher concentrations of the vitrification solution. Therefore, higher concentrations of the vitirification solution do not seem to cause any problems in vitrification procedure. II. The effects of vitrification method. 1-cell zygotes showed the survival rate between 78.8% and 92.4%. The lowest and the highest survival rate was observed in the 6.0 M and 4.0 M vitrification solution, respectively. 2-cell development rates varied from 77.6% to 91.3%. Blastocyst development rate was shown highest in 5.0 M and the lowest in 4.0 M solution. Therefore, the highest 2-cell and blastocyst development rate was observed in 5.0 M solution. III. Comparison of vitrification and slow freezing-thawing method on 1-cell zygotes. This experiment showed that 1-cell zygotes had the highest survival and development rates in 5.0 M vitrification solution. Vitrified group of 1-cell zygotes, in the 5.0 M vitrification solution, were compared with the group processed in slow freezing-thawing method. The development rate into 2-cell and blastocyst as well as the survival rate were higher in the vitrified group than in the slowly freezed group. Conclusion: 1. The results demonstrate that the best cryoprotectant is a 5.0 M vitrification solution for 1-cell zygotes. 2. Vitrification method significantly increases the survival rate of the 1-cell zygote and its development into 2-cell and blastocyst. Equilibration and exposure time during the vitrification was remarkerbly short in this experiment. Total time, from the exposure to vitirification solution to storage in the liquid nitrogen, was taken only 90 seconds. In contrast, the slow freezing-thawing method have taken more than four hours. Taken together, we presume that the overall time used for the procedure contributes to the results as an important parameter. 3. The loading of 1-cell zygotes on the EM grid is technically more simple and takes less time than the straw or cryo vial method.

• Journal of Korean Academy of Nursing
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• v.5 no.2
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• pp.38-50
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• 1975

This study was conducted to find the shortest optimum time for taking oral temperature and axillary temperature, which does not affect reliability of body temperature. For this purpose, first, the time at which all the samples are reaching maximum temperature is identified Second, the mean maximum temperature is compared with the mean temperature of each consecutive measurement by T-test to find the time at which no significant changes in temperature occurs along time sequence. Third, optimum temperatures are set at points of -0.2℉, -0.4℉, -0.6℉, -0.8℉, -1.0℉, -1.2℉, -1.4℉, from maximum temperature. A point of time at which 90% of samples reach at optimum temperature is identified and defined as optimum time. The study sample, a total of 164 cases were divided into two groups according to their measured body temperature. The group with body temperature below 37 $^{\circ}C$(A group) and above 37$^{\circ}$1'C (B group) were compared on the time required to reach maximum temperature and optimum temperature. The results are as follow. 1. The time required for total sample to reach maximum temperature was 13 minutes in both groups by oral method, 15 minutes in A group and 13 minutes in B group by axillary method. Time required for 90 % of cases reach maximum temperature by oral method was 10 minutes in both group. By axillary method, 12 minutes in A group. (Ref: table 2) 2. Statistical analysis by means of T-test, the time which does not show a significant change by oral method were 12 minutes in A group and 11 minutes in B group, and by axillary method 14 minutes in A group and 11 minutes in B group. (Ref: table 5, 6.) 3. Where optimum temperature was defined as maximum temperature minus 0.2 ℉, optimum time was found 8 minutes in both groups by oral method, and 11 minutes in A group and 9 minutes in B group by axillary method 4. Where optimum temperature was defined as maximum temperature minus 0.4 ℉, optimum time was found 7 minutes in A group and 6 minutes in B group by oral method, and 9 minutes in A group and 7 minutes in B group by axillary method 5. Where optimum temperature was defined as maximum temperature minus 0.8 ℉, optimum time was found 6 minutes in A group and 6 minutes in B group by axillary method (Ref: table 7, 8, 9, 10) 6. The commonly practiced temperature taking time, 3 minutes in oral method and 5 minutes in axillary method can be accepted as pertinent when physiological variation of body temperature at the mean level of -1, 2 ℉ is accepted. 7. The difference in time required to resister maximum temperature was compared between the group with body temperature below 37$^{\circ}C$ and above 37$^{\circ}$1'C, and found no significant difference in oral mettled and 1 - 4 minute difference in axillary method with shorter time requirement in feverish group.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.11 no.1
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• pp.1-13
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• 2008

This study review of Compositional Evaluation Items for Environmental Conservation Value Assessment Map (ECVAM) in Korea. The ECVAM is composed of legal assessment and environmental/ecological assessment items. ECVAM basically adapts an overlay method for environmental/ecological assessment items. The objective of this study is to suggest supplementary items for the ECVAM with the following process : Overlapping rates of the assessment items in the ECVAM are calculated to understand the grade distribution of the environmental conservation value assessment and to analyze the overlapping rates among the assessment items, as a result it is found that various items are overlapped each other. In order to reflect effectively each assessment item to the ECVAM, Analyzed the overlapping degree among assessment items to be applied to this map. On the concrete we gripped results to be assessed by various items, which were overlapped each other. In order to reflect effectively each assessment item to the environmental conservation value assessment map of national land, we analyzed the overlapping degree on environmental/ecological items, and investigated the grade distribution by field survey. In this study we assessed the ECVAM by 5 kinds of method. Method 1 is Grade 1 areas of each administrative district, Method 2 is Comparing overlapping areas of each assessment items Grade 1, 2 and Permission of each assessment items' duplication, Method 3 is Grade 1, 2 areas by only singular assessment items, Method 4 is Only Grade 1 areas of Method 2 and Method 5 is Only Grade 2 areas of Method 2. As results, Method 1 showed Seoul and other metropolitan cities reveal a high proportion of Grade I regions by the legal assessment items. Kangwon-Do, show a high proportion of Grade I regions by the environmental/ecological assessment item. Method 2 showed 93.4% of diameter Grade II(standard for stability), forest diameter item was accounted for 99.9% by Method 3, Method 4 showed 95.7% of forest diameter and forest density was accounted for 66.4% by Method 5. From now on, this study will contribute to reduce the complexity in the process of manufacturing ECVAM of National Land, and to raise the pliability in the process of managing and updating this map.

• Archives of Pharmacal Research
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• v.15 no.2
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• pp.109-114
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• 1992

For the risk assessment of human exposure to volatile halogenated hydrocarbons, a dynamic purge trap/on-column cryofocusing method using capillary gas chromatograph-$^{63}Ni$ electron capture detector and thermal desorption unit was applied to analyze the free forms, metabolites of 1, 1, 2-trichloroethylene and 1, 1, 2, 2-tetrachloroethylene. The urine sample was diluted with distilled water, hydrolyzed and sealed. Then the inert gas was infused to purge out free 1, 1, 2-trichloroethylene, free 1, 1, 2, 2-tetrachloroethylene and urichloroethanol. These compounds were trapped to $Tenax^R$ / GC-gas trap device throughout clean up tube. Being undertectable to gas chromatograph directly, trichloroacetic acid was methyl esterificated and trapped in the manner above mentioned. The optimal incubation time to get best recovery of methyl ester was 4 hours at $60^circ$C. The concentrations of free volatile halogenated hydrocarbons and their metabolites in urine were obtained of free volatile halogenated hydrocarbons and their metabolites in urine were obtained from 5 healthy volunteers. This analytical method is expected to make the biological monitoring more precise and convenient.

• Journal of the Korean institute of surface engineering
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• v.46 no.1
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• pp.16-21
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• 2013

CIGS (Cu-In-Ga-Se) films were deposited on the Mo coated soda lime glass (Mo/SLG) by RF magnetron sputtering using a single sintered target with different chemical compositions. Heat treatment of the CIGS films were carried out under three different conditions, 1step ($350^{\circ}C$ for 2 hour and $550^{\circ}C$ for 2 hour) and 2step ($350^{\circ}C$ for 1 hour and $550^{\circ}C$ for 1 hour). In the case of CIGS films post-annealed on 2step method, grain size remarkably increased compared to other methods, indicating that chemical composition [Cu/(Ga+In) = 1] of CIGS films was same as CIGS target. After heat treatment by 2step method, band gap energy of the CIGS film deposited at RF 80 W showed 1.4 eV which is broadly similar to identical band gap energy (1.2 eV) of CIGS film prepared by evaporation method. Therefore, 2step heat treatment method could be expected to low temperature process.

• Journal of Digital Contents Society
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• v.16 no.5
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• pp.671-680
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• 2015

The Joint Bayesian[1] method was published in 2012. Since then, it has been used for binary classification in almost all state-of-the-art face recognition methods. However, no improved methods have been published so far except 2D-JB[2]. In this paper we propose an improved version of the JB method that considers the features of both the given face image and its mirror image. In pattern classification, it is very likely to make a mistake when the value of the decision function is close to the decision boundary or the threshold. By making the value of the decision function far from the decision boundary, the proposed method reduces the errors. The experimental results show that the proposed method outperforms the JB and 2D-JB methods by more than 1% in the challenging LFW DB. Many state-of-the-art methods required tons of training data to improve 1% in the LFW DB, but the proposed method can make it in an easy way.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2001.11a
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• pp.112-119
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• 2001

Parameter tuning methods by Ziegler-Nickels for control systems are generally classified into Z-N(1) and Z-N(2). The purpose of this paper is to describe what relations exist between methods of Z-N(1) and Z-N(2), or how Z-N(1) method can be originated from Z-N(2) method by analyzing one loop control system of P or PI controller and time delay process. The formulas of Z-N(1) consist of process parameters, L(time delay), $K_m$(gain) and $T_m$(time constant), but Z-N(2) method is based only on the ultimate gain $K_u$ and the ultimate period $T_u$ acquired normally by practical trial without any parameters of Z-N(1). In this paper, for the first step to seek mutual relations, the simple formulas of Z-N(2) are transformed into the formulas composed of the same parameters as Z-N(1) which is derived from the analysis of frequency characteristics. Then, the approximation of the actual ultimate frequency is proposed as important premise in the translation between Z-N(1) and (2). Such equalization and approximation brings a simple approximated formula which can explain how Z-N(1) is originated from the Z-N(2) in the form of formula. And a model system is adopted to compare the approximated formula to Z-N(1) and Z-N(2) methods, the results of which show the effectiveness of the proposals.

• Journal of Korea Society of Digital Industry and Information Management
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• v.18 no.2
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• pp.17-26
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• 2022

In this paper, we propose a novel method of performing convolutional operations on a 2-D Processing Element(PE) array. The conventional method [1] of mapping the convolutional operation using the 2-D PE array lacks flexibility and provides low utilization of PEs. However, by mapping a convolutional operation from a 2-D PE array to a 1-D PE array, the proposed method can increase the number and utilization of active PEs. Consequently, the throughput of the proposed Deep Convolutional Neural Network(DCNN) accelerator can be increased significantly. Furthermore, the power consumption for the transmission of weights between PEs can be saved. Based on the simulation results, the performance of the proposed method provides approximately 4.55%, 13.7%, and 2.27% throughput gains for each of the convolutional layers of AlexNet, VGG16, and ResNet50 using the DCNN accelerator with a (weights size) x (output data size) 2-D PE array compared to the conventional method. Additionally the proposed method provides approximately 63.21%, 52.46%, and 39.23% power savings.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.109-113
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• 2003

Cryopreservation of embryogenic callus derived from apical meristem culture was attempted by slow prefreezing method (two-step method) with various cryoprotectants in sweetpotato cv. 'Yulmi' Precultured embryogenic calli on medium containing 10 mg/L ABA prior to slow prefreezing in liquid nitrogen indicated higher survival rate than 1.0 mg/L ABA preteatment. The cryoprotectant comprising 1.28 M DMSO in 0.4 M sucrose solution gave the best survival (over 46%) of sweetpotato cells exposed to liquid nitrogen as determined by TTC reduction and FDA staining method. Cryopreserved calli cultured on MS medium with 1.0 mg/L 2,4-D were grown for 4 weeks in the dark and induced embryos after another 4 weeks. They were subcultured on MS medium supplemented with 0.1 mg/L 2,4-D＋0.1 mg/L kinetin for 2 weeks and regenerated into normal plantlets in MS basal medium.

• Journal of Pharmacopuncture
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• v.11 no.1
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• pp.177-187
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• 2008

Objectives : This study was performed to analyze the effective components of essential oil obtained from Yongdamsagantang, which has been efficacious against leukorrhea in gynecologic diseases. Methods : I obtained the essential oils of Yongdamsagan-tang by hydrodistillation extraction method and supercritical fluid extraction(SFE) method, and then I analyzed those by GC/MS(Gas Chromatography/Mass Spectrum). Results : 1. The optimum SFE(Supercritical Fluid Extraction) condition was obtained in the following experiment conditions: pressure 200atm, temperature $45^{\circ}C$, duration of extraction 25minutes. 2. With GC(Gas Chromatography) and GC/MS(Gas Chromato- graphy/Mass Spectrum) analysis, I identified 37 compounds in the Yongdamsagan-tang's essential oil obtained through the SFE method. The main compounds were as follows : 3-Methyl-but-2-enoic acid,2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H -pyrano[3,2-g]chromen-3-yl ester(49.81%), (Z)-6-Pentadecen-1 -ol(3.19%), (-)-Spathulenol(2.40%). 3. I identified 4 compounds in the Yongdamsagan-tang's essential oil obtained through the hydrodistillation method. The main compounds were as follows : 3-Methyl-but-2-enoic acid, 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano [3,2-g]chromen-3-yl ester(2.61%). 4. 3-Methy I-but-2-enoic acid, 2,2-dimethyl-8-oxo-3,4-dihydro-2H,8H-pyrano[3,2-g] chromen-3-yl ester, all were identified in both the SFE method and the hydrodistillation method, but the others were not identified in common. 5. I also conducted an additional test in order to examine the essential oil's antimicrobial action against bacteria. Both MIC(Minimum Inhibitory Concentrations) and MBC(Minimum Bactericidal Concentrations) were $0.125mg/m{\ell}$ against N. meningitidis, however MIC and MBC were $1.0mg/m{\ell}$ in antimicrobial action against 12 different genera of bacteria.