• Asian-Australasian Journal of Animal Sciences
• /
• 제19권5호
• /
• pp.632-637
• /
• 2006

Oviduct-specific glycoprotein 1 (OVGP1) is implicated in playing a role in fertilization and early embryo development. In this study, we have obtained the sequence of intron 9 of OVGP1 gene in swine. Comparative sequencing of Meishan (a native Chinese breed) and Large White pig breeds revealed an A/T substitution at position 943. A PCR-EcoRI-RFLP assay was developed to detect this mutation. Polymorphism analysis in Qingping animals showed that pigs with BB genotype had lower number of piglets born alive (NBA) in multiple parities than pigs with AA (p<0.05) and AB genotype (p<0.01). In Large $White{\times}Meishan$ ($LW{\times}M$) $F_2$ offspring, the weight of both ovaries (OW) of the BB genotype was significantly lighter than that of AB (p = 0.05) and AA (p<0.01) genotypes. Analysis of the data also revealed that the mutation locus affected these two traits mostly by additive effects. These studies indicated that the polymorphism was associated with NBA and OW in two distinct populations and further investigations in more purebreds or crossbreds are needed to confirm these results.

• Journal of Korean Academy of Nursing
• /
• 제21권3호
• /
• pp.323-338
• /
• 1991

This study investigated the effect of supportive nursing care including modified guided imagery on the discomfort, self- esteem, erythrocyte semdimentation rate(ESR) and quality of life(Q. L.) in persons with rheumatoid arthritis (R. A.). The purpose was to contribute to the development of theoretical nursing care to enhance the Q. L. of persons with chronic illness. From Nov. 22, 1990 to May 2, 1991, 49 patients registered at a rheumatic clinic at one general hospital in Seoul, were accepted according to the research criteria as subjects for this study. Among these, 26 were selected for an experimental group to he treated with an interpersonal interview and modified guided imagery, the other 23 became the control group not treated for the purpose of measuring and comparing the effect of the treatment. Statistical analysis was done by a SAS program for X$^2$-test, unpaired t -test, Pearson correlation coefficients and factor analysis. The results were as follows : 1. The pre-experimental discomfort level of the total subject group averaged 25.42(S. D. ＝8.77), and the difference between the pre /post experimental discomfort levels of the two groups was not statistically significant. 2. The pre - experimental self- esteem scores of the total subject group averaged 27.48(S. D. ＝3.95), and the difference between the pre /post experimental self -esteem scores of the two groups was not statistically significant. 3. The pre - experimental Q. L. scores of the total subject group averaged 28.70(S. D. ＝5.99), and the difference between the pre /post experimental Q. L. score of the two groups was statistically significant (t＝2.1748, df＝47, p＝ .0347), 4. The pre - experimental mean of the ESR of the total subject group was 34.48(S. D. ＝19.50) mm, and the difference between the pre /post expeimental ESR scores of the two groups was not statistically significant. 5. The Family support scores of the total subject group averaged 41.28(S. D. ＝10.27). 6. The total subject group 0.L. score was correlated with self-exteem score (r＝ .3984, p＝ .0046). In this study, supportive nursing care including modified guided imagery increased the quality of life scores for this group of persons with R. A. significantly. So the concept of supportive nursing care of this study may be effective in enhancing the quality of life persons with chronic illness. Further investigations related to the methodology and with other groups of clients was recommended.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권20호
• /
• pp.8893-8900
• /
• 2014

Dysregulated expression of microRNAs (miRNAs) has been shown to be closely associated with tumor development, progression, and carcinogenesis. However, their clinical implications for gastric cancer remain elusive. To investigate the hypothesis that genome-wide alternations of miRNAs differentiate gastric cancer tissues from those matched adjacent non-tumor tissues (ANTTs), miRNA arrays were employed to examine miRNA expression profiles for the 5-pair discovery stage, and the quantitative real-time polymerase chain reaction (qRTPCR) was applied to validate candidate miRNAs for 48-pair validation stage. Furthermore, the relationship between altered miRNA and clinicopathological features and prognosis of gastric cancer was explored. Among a total of 1,146 miRNAs analyzed, 16 miRNAs were found to be significantly different expressed in tissues from gastric cancer compared to ANTTs (p<0.05). qRT-PCR further confirmed the variation in expression of miR-193b and miR-196a in the validation stage. Down-expression of miR-193b was significantly correlated with Lauren type, differentiation, UICC stage, invasion, and metastasis of gastric cancer (p<0.05), while over-expression of miR-196a was significantly associated with poor differentiation (p=0.022). Moreover, binary logistic regression analysis demonstrated that the UICC stage was a significant risk factor for down-expression of miR-193b (adjusted OR=8.69; 95%CI=1.06-56.91; p=0.043). Additionally, Kaplan-Meier survival curves indicated that patients with a high fold-change of down-regulated miR-193b had a significantly shorter survival time (n=19; median survival=29 months) compared to patients with a low fold-change of down-regulated miR-193b (n=29; median survival=54 months) (p=0.001). Overall survival time of patients with a low fold-change of up-regulated miR-196a (n=27; median survival=52 months) was significantly longer than that of patients with a high fold-change of up-regulated miR-196a (n=21; median survival=46 months) (p=0.003). Hence, miR-193b and miR-196a may be applied as novel and promising prognostic markers in gastric cancer.

• Clinics in Shoulder and Elbow
• /
• 제25권4호
• /
• pp.296-303
• /
• 2022

Background: A previous study reported that hyperlipidemia increases the incidence of tears in the rotator cuff tendon and affects healing after repair. The aim of our study was to compare the gene and protein expression of torn rotator cuff tendons in patients both with and without hypercholesterolemia. Methods: Thirty patients who provided rotator cuff tendon samples were classified into either a non-hypercholesterolemia group (n=19, serum total cholesterol [TC] <200 mg/dL) and hypercholesterolemia group (n=11, serum TC ≥240 mg/dL) based on their concentrations of serum TC. The expression of various genes of interest, including COL1A1, IGF1, IL-6, MMP2, MMP3, MMP9, MMP13, TNMD, and TP53, was analyzed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, Western blot analysis was performed on the proteins encoded by interleukin (IL)-6 and TP53 that showed significantly different expression levels in real-time qRT-PCR. Results: Except for IGF1, the gene expression levels of IL-6, MMP2, MMP9, and TP53 were significantly higher in the hypercholesterolemic group than in the non-hypercholesterolemia group. Western blot analysis confirmed significantly higher protein levels of IL-6 and TP53 in the hypercholesterolemic group (p<0.05). Conclusions: We observed an increase in inflammatory cytokine and matrix metalloproteinase (MMP) levels in hypercholesterolemic patients with rotator cuff tears. Increased levels of IL-6 and TP53 were observed at both the mRNA and protein levels. We suggest that the overexpression of IL-6 and TP53 may be a specific feature in rotator cuff disease patients with hypercholesterolemia.

• Reproductive and Developmental Biology
• /
• 제30권1호
• /
• pp.13-19
• /
• 2006

To search of new porcine pheromonal odorants for biostimulation control system technologies to improve reproductive efficiency in livestock species, the comparative molecular field analysis (CoMFA) for binding affinity constant $(p(Od)_{50})$ between porcine odorant binding protein (pOBP) and ligands of odorant 2-(cyclohexyloxy) tetrahydrofurane derivatives as substrate molecule was conducted and discussed. In the optimized CoMFA model AIV with chirality $(C_1'(R),\;C_2(S))$ in substrate molecule and atom based fit alignment (A) of odorants, the statistical results showed the best predictability of the binding affinities $(p(Od)_{50})$ based on the LOO cross-validated value $r^2_{cv}.\;(q^2=0.886)$ and non-cross-validated conventional coefficient $(r^2_{ncv}.=0.984)$. the binding affinity constants exhibited a good correlation with steric (40.8%), electrostatic (14.6%) and hydrophobic (44.6%) factors of the substrate molecules. from the analytical results of the contour maps, which may give us some valuable informations to the modification of odorants for effective binding affinity.

• Journal of Oral Medicine and Pain
• /
• 제26권1호
• /
• pp.17-25
• /
• 2001

The purpose of this study is to evaluate the relationship of menstrual cycle and halitosis by measuring the concentrations of Voltile Sulfur Compounds, secretion rate of unstimulated saliva, secretion rate of stimulated saliva and viscosity of saliva during the menstrual cycle. The subjects were 19 female dental students of Yonsei University who had relatively good alignment of the teeth. They hadn't taken antibiotics or oral contraceptive pills during the few months prior to the experiment, and they didn't have any dental caries involving the pulp or periodontal disease. Lady-$Q^{(R)}$(Alpain Korea, Korea), which confirms the ovulation using saliva, was used to find out the menstrual cycle of subjects. Their history was taken and their basal body temperature was measured. On the basis of these data, the amount of Volatile Sulfur Compounds, secretion rate of unstimulated saliva, secretion rate of stimulated saliva, viscosity of saliva were measured during 1 day of the proliferative phase, 3 days of ovulatory phase and 1 day of the luteal phase within the menstrual cycle. The results were as follows : 1. The amount of Volatile Sulfur Compounds, secretion rate of unstimulated saliva, secretion rate of stimulated saliva, and viscosity of saliva showed no statistically significant cyclic change during proliferative phase, ovulatory phase, and luteal phase(p<0.05). 2. Between the secretion rate of unstimulated saliva and secretion rate of stimulated saliva, there was significant correlation during proliferative phase and luteal phase(p<0.05) and there was no significant correlation during ovulatory phase but relatively close result was seen. 3. The amount of Volatile Sulfur Compounds during proliferative phase and luteal phase had statistically significant correlation(p<0.05). 4. Secretion rate of stimulated saliva during proliferative phase and ovulatory phase, proliferative phase and luteal phase, ovulatory phase and luteal phase had significant correlations (p<0.01).

• Fisheries and Aquatic Sciences
• /
• 제19권5호
• /
• pp.23.1-23.7
• /
• 2016

The effects of daily feeding frequency (Exp I), water temperature (Exp II), and stocking density (Exp III) on the growth of river puffer, Takifugu obscurus, juvenile fish of 10 and 40 g in body weight were examined to develop effective techniques to produce river puffer in a non-exchange water system. In Exp I, fish were fed commercial floating feed with 45 % protein one to five times per day to apparent satiation each by hand daily for 8 weeks at $25^{\circ}C$. In both the 10- and 40-g size groups, the final body weight, daily feed consumption, and weight gain of fish fed one meal per day were significantly lower than those of fish fed five meals per day (P < 0.05). However, there were no significant differences in the final body weight, daily feed consumption, and weight gain among fish fed two, three, and five meals per day. Feed efficiency showed decreasing tendency with increasing size of fish. In Exp II, fish of 10 and 40 g in initial body weight were reared with the commercial feed at $15-30^{\circ}C$ for 8 weeks. The weight gain of fish increased with raising water temperature up to $25^{\circ}C$ and decreased drastically at $30^{\circ}C$ for both sizes. The Q10 of specific growth rate was decreased with raising water temperature from 5.04 (temperature interval, $15-20^{\circ}C$) to 0.66 ($25-30^{\circ}C$) for the 10-g fish and from 4.98 to 0.31 for the 40-g fish. In Exp III, the effect of stocking density on growth was examined with fish of 10 and 40 g in initial body weight. The final body weight for initial stocking densities of 4, 8, and $12kg/m^3$ was significantly higher than that of $20kg/m^3$ for the 10-g fish, and the final stocking density reached 10.1, 19.2, 28.7, and $39.9kg/m^3$, respectively. For the 40-g fish, the final body weight for initial stocking densities of 3 and $6kg/m^3$ was significantly higher than that of 9 and $15kg/m^3$ and the final stocking density reached 7.38, 13.5, 17.1, and $27.5kg/m^3$, respectively (P < 0.05). In both groups, weight gain tended to decrease with increasing stocking density; however, survival showed no significant difference.

• Korean Journal of Community Nutrition
• /
• 제23권6호
• /
• pp.525-537
• /
• 2018

Objectives: This study was conducted to examine the relationship between white rice and Kimchi intakes and the risk of metabolic syndrome (Mets) in Korean adults. Methods: Dietary intake and health data of 8289 subjects aged 19 years and over from the 2013-2015 Korea National Health and Nutrition Examination Survey (KNHANES) were used. Daily total intake of white rice and Kimchi was assessed by 24-hour recall data. Multivariate logistic regression analysis was used to estimate the risk of Mets according to the daily intake of white rice and Kimchi. Results: The highest intake of white rice and Kimchi was associated with a higher risk of metabolic syndrome (Q1 vs Q5, multivariable adjusted OR=1.45, 95% CI: 1.03-2.03) in women. In addition, a significantly increased risk of elevated blood pressure (multivariable adjusted P for trend 0.0459) was associated with a higher intake of white rice and Kimchi in women. There was no significant trend in the risk of metabolic syndrome according to the intake of white rice and Kimchi in men. Conclusions: A higher intake of white rice and Kimchi was only associated with an increased risk of metabolic syndrome in women indicating it is necessary to consume more various food groups beside white rice and Kimchi, especially in women.

• Ocean and Polar Research
• /
• 제32권3호
• /
• pp.229-236
• /
• 2010

A study was conducted to investigate the effect of water temperature and photoperiod on the oxygen consumption of the fasting juvenile Pacific cod Gadus macrocephalus (mean body weight 79.9${\pm}$2.0 g) in order to quantify metabolic response of the species under given conditions. The oxygen consumption rate (OCR) of G. macrocephalus was measured under a combination of four different water temperatures (7, 10, 13 and $16^{\circ}C$) and three different photoperiods (24L:0D, 12L:12D and 0L:24D) with an interval of 5 minutes over a 24-hour period using a closed recirculating respirometer. Three replicates were set up in each treatment. OCRs increased with increased water temperatures under all photoperiod conditions (P<0.001). Mean OCRs at 7, 10, 13 and 16oC ranged from 793.7~1108.4, 1145.7~1570.3, 1352.8~1742.5 and 1458.2~1818.6 mg $O_2$ $kg^{-1}$ $h^{-1}$, respectively. Under all water temperature conditions except $7^{\circ}C$ (P<0.001), mean OCRs of G. macrocephalus were the highest in continuous light (24L:0D) followed by 12L:12D and 0L:24D photoperiods. Mean OCRs of fish exposed to the 12L:12D photoperiod were significantly higher during the light phase than during the dark phase under all temperature conditions (P<0.001). $Q_{10}$ values ranged from 3.19~5.13 between 7 and $10^{\circ}C$, 1.41~1.74 between 10 and $13^{\circ}C$ and 1.15~1.35 between 13 and $16^{\circ}C$, respectively. Based on overall results, water temperature, photoperiod and their combinations exerted a significant influence on the metabolic rate of juvenile cod. This study provides empirical data for estimating the amount of oxygen demand and managing the culture of cod under the given water temperatures and photoperiods.

• Journal of Genetic Medicine
• /
• 제15권2호
• /
• pp.64-71
• /
• 2018

Purpose: Overgrowth syndromes are conditions that involve generalized or localized areas of excess growth. In this study, the clinical, molecular, and genetic characteristics of Korean patients with overgrowth syndrome were analyzed. Materials and Methods: We recruited 13 patients who presented with overgrowth syndrome. All patients fulfilled inclusion criteria of overgrowth syndrome. Analysis of the clinical and molecular investigations of patients with overgrowth syndrome was performed retrospectively. Results: Among the 13 patients with overgrowth syndrome, 9 patients (69.2%) were found to have molecular and genetic causes. Among the seven patients with Sotos syndrome (SS), two had a 5q35microdeletion that was confirmed by fluorescent in situ hybridization. In two patients with SS, intragenic mutations including a novel mutation, c.5993T>A (p.M1998L), were found by Sanger sequencing. One patient had one copy deletion of NDS1 gene which was confirmed by multiplex ligation-dependent probe amplification. Among five patients with Beckwith-Wiedemann syndrome, three had aberrant imprinting control regions; 2 hypermethylation of the differentially methylated region of H19, 1 hypomethylation of the differentially methylated region of Kv. In one patient displaying overlapping clinical features of SS, a de novo heterozygous deletion in the chromosomal region 7q22.1-22.3 was found by single nucleotide polymorphism-based microarray. Conclusion: Considering high detection rate of molecular and genetic abnormalities in this study, rigorous investigations of overgrowth syndrome may be an important tool for the early diagnosis and genetic counseling. A detailed molecular analysis of the rearranged regions may supply the clues for the identification of genes involved in growth regulation.