• Nutrition Research and Practice
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• v.10 no.4
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• pp.377-384
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• 2016

BACKGROUND/OBJECTIVES: Resveratrol, a natural polyphenol, has multiple functions in cellular responses including apoptosis, survival, and differentiation. It also participates in the regulation of inflammatory response and oxidative stress. MicroRNA-Let-7A (miR-Let7A), known as a tumor suppressor miRNA, was recently reported to play a crucial role in both inflammation and apoptosis. Therefore, we examined involvement of miR-Let7A in the modulation of inflammation and cell survival/apoptosis regulated by resveratrol. MATERIALS/METHODS: mRNA expression of pro-/anti-inflammatory cytokines and sirtuin 1 (SIRT1), and protein expression of apoptosis signal-regulating kinase 1 (ASK1), p-ASK1, and caspase-3 and cleaved caspase-3 were measured, and cell viability and Hoechst/PI staining for apoptosis were observed in Lipopolysaccharide (LPS)-stimulated human THP-1 macrophages with the treatment of resveratrol and/or miR-Let7A overexpression. RESULTS: Pre-treatment with resveratrol ($25-200{\mu}M$) resulted in significant recovery of the reduced cell viabilities under LPS-induced inflammatory condition and in markedly increased expression of miR-Let7A in non-stimulated or LPS-stimulated cells. Increased mRNA levels of tumor necrosis $factor-{\alpha}$ and interleukin (IL)-6 induced by LPS were significantly attenuated, and decreased levels of IL-10 and brain-derived neurotrophic factor were significantly restored by resveratrol and miR-Let7A overexpression, respectively, or in combination. Decreased expression of IL-4 mRNA by LPS stimulation was also significantly increased by miR-Let7A overexpression co-treated with resveratrol. In addition, decreased SIRT1 mRNA levels, and increased p-ASK1 levels and PI-positive cells by LPS stimulation were significantly restored by resveratrol and miR-Let7A overexpression, respectively, or in combination. CONCLUSIONS: miR-Let7A may be involved in the inflammatory response and cell survival/apoptosis modulated by resveratrol in human THP-1 macrophages.

• Analytical Science and Technology
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• v.17 no.6
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• pp.464-469
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• 2004

Cyclic voltammetry was used for elucidating the electrochemical behavior of Ru-cupferron complex in 1 mM phosphate buffer. The optimal conditions of ruthenium were found to be 1 mM phosphate buffer solution (pH 6.0) containing 0.1 mM cupferron, scan rate of 100 mV/s. By using the plot on the reduction peak currents of linear scan voltammograms vs. ruthenium concentration, the detection limit ($3{\sigma}$) was $1.2{\times}10^{-7}M$.

• Journal of Bio-Environment Control
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• v.25 no.4
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• pp.302-307
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• 2016

The aim of this study was to examine the optimum wattage and installation height using nano-carbon fiber infrared heating lamp (NCFIHL) for heating energy saving and plug seedling production in plug seedling production greenhouse in winter season. NCFIHL of 700 and 900 W was installed over the bed ($1.2{\times}2.4m$) as 0.7, 1.0, and 1.3 m height, respectively, for the production of grafted watermelon seedling in venlo-type glasshouse. Watermelon (Citrullus lanatus (Thunb.) Manst.) 'Jijonggul' and gourd (Lagenaria leucantha Rusby.) 'Sunbongjang' were used as scions and rootstocks, respectively. The scions and rootstocks were grafted by single cotyledon ordinary splice grafting. Light intensity of NCFIHL was below the $1{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in all treatment. Spectral distributions of NCFIHL presented mostly infrared area. When outside air temperature was below $10^{\circ}C$, 700 and 900 W NCFIHL installed with 0.7 m height treatment and 900 W NCFIHL installed with 1.0 m height treatment maintained the setting air temperature ($20^{\circ}C$) at night. In the result of taking thermal imaging, the grafted watermelons were getting warm fast in 900 W NCFIHL installed with 0.7 m height treatment at night. Compactness of the grafted watermelons was the greatest in 700 W NCFIHL installed with 1.3 m height treatment. The results indicate that NCFIHL installed above 1.0 m height using 700 W was suitable for production of plug seedling.

• Horticultural Science & Technology
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• v.30 no.5
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• pp.568-572
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• 2012

Determination of ploidy level for the mother plant is prerequisite for effective breeding. The study was carried out to determine the ploidy level in 7 different plant materials by FISH karyotype analysis. Among the seven plant varieties analyzed, all exhibit tetraploid (2n = 4x = 28) based on the results observed in chromosome analysis. Four signals of 45S rDNAs were detected on the terminal region of the short arm of chromosome 7. The length of somatic metaphase chromosomes ranges from 1.67 to $2.67{\mu}m$ in 'Alexandra', 1.40 to $2.04{\mu}m$ in 'Freud', 1.64 to 2.24 in 'Little Silver', 1.69 to $2.26{\mu}m$ in 'Teresa', 1.70 to $2.65{\mu}m$ in 'Tineke', 1.35 to $2.08{\mu}m$ in 'Vital', 1.39 to $2.04{\mu}m$ in 'Yellow Mimi'. Total length of the chromosome ranges from $11.23{\mu}m$ in 'Freud' as minimum to $15.05{\mu}m$ in 'Alexandra' as maximum. The karyotypes were composed of metacentric, submetacentric, and subtelocentric chromosome but there is no subtelocentric chromosome.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05b
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• pp.630-634
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• 2004

This paper describes the design of a two stage 1V power supply SiGe Low Noise Amplifier operating at 5.25 GHa for 802.lla wireless LAN application. The achieved performance includes a gain of 17 ㏈, noise figure of 2.7㏈, reflection coefficient of 15 ㏈, IIP3 of -5 ㏈m, and 1-㏈ compression point of -14㏈m. The total power consumption of the circuit was 7 mW including 0.5mW for the bias circuit.

• Herbal Formula Science
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• v.16 no.2
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• pp.155-162
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• 2008

Objectives : The present study was designed to investigate corelation between mix proportion of Scutellaria baicalensis (SB) and Coptis chinensis (CC) on lipopolysaccharide (LPS)-induced TYPE-1 interferon production. Methods : I examined TYPE-1 interferon, interferon regulating factor (IRF)-1,7 and interleukin(IL)-10 production on LPS-induced RAW264.7 cells to evaluate inhibitory effect of mix proportion of SB and CC using real time PCR. Results : Mixture of SB and CC regulated TYPE-1 interferon and IRF-1,7 mRNA expression with SB dose dependent manner, while maintained IL-10 mRNA expression on LPS-induced RAW264.7 cells. Conclusion : In mixture of SB and CC, SB plays a key role in reducing TYPE-1 interferon through inactivation IRF-1,7. Furthermore mixture of SB and CC maintained IL-10 mRNA level. Collectively, this results suggest that SB confer beneficial effects in autoimmune diseases clinically.

• Advances in environmental research
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• v.3 no.3
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• pp.207-216
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• 2014

A study was conducted to determine the phytotoxic effect of mercury on seed germination and seedling growth of an important arid legume tree Albizia lebbeck. The seeds germination and seedling growth performance of A. lebbeck responded differently to mercuric chloride treatment (1 mM, 3 mM, 5 mM and 7 mM) as compared to control. Seed germination of A. lebbeck was significantly (p < 0.05) affected by mercury treatment at 1 mM. Root growth of A. lebbeck was not significantly affected by mercury treatment at 1 mM, and 3 mM. Shoot and root length of A. lebbeck were significantly (p < 0.05) affected by 5 mM concentration of mercury treatment. Increase in concentration of mercury treatment at 5 mM and 7 mM significantly (p < 0.05) reduced seedling dry weight of A. lebbeck. The treatment of mercury at 1 mM decreased high percentage of seed germination (22%), seedling length (10%), root length (21.85%) and seedling dry weight (9%). Highest decrease in seed germination (51%), seedling (34%), root length (48%) and seedling dry weight (41%) of A. lebbeck occurred at 7 mM mercury treatment. A. lebbeck showed high percentage of tolerance (78.14%) to mercury at 1 mM. However, 7 mM concentration of mercury produced lowest percentage of tolerance (51.65%) in A. lebbeck. The seed germination potential and seedling vigor index (SVI) clearly decreased with the higher level of mercury. Plantation of A. lebbeck in mercury-polluted area will help in reducing the burden of mercury pollution. A. lebbeck can serve better in coordinating in land management programs in metal contaminated areas. The identification of the toxic concentration of metals and tolerance indices of A. lebbeck would also be helpful for the establishment of air quality standard.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.13 no.3
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• pp.95-101
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• 1980

Optical properties were studied at the fishing ground of anchovy in the southern part of Korea based on seven oceanographic stations from May to August and November to December, 1977. Submarine daylight intensity was measured at intervals of 1m depth in the upper 30 m layer by the underwater luxmeter (Toshiba # 9). The absorption coefficient of the sea water ranged from 0.066 to 0.619 (mean 0.21) for six months. The transparency ranged from 2.6 to 16 meters (mean 8.4m). The relationship between absorption coefficient (n) and transparency (D) was k=1.70/D. The mean water color in this area was 4.8 $(3\~10)$ in Forel scales. The rates of light penetration for daylight at four different depths were computed with reference to the surface light intensity. The mean rates of light penetration were $69.38\%(25.43\~88.10\%),\;30.35\%\;(4.38\~59.46\%),\;12.53\%\;(0.75\~33.51\%),\;5.7\%(0.18\~20.27\%)$ at depths of 1, 5, 10 and 15 m respectively.

• Journal of Embryo Transfer
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• v.27 no.4
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• pp.253-258
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• 2012

This study was carried out to effects of ethylene glycol concentration, sucrose and culture day of in vitro production embryo on slow-down freezing in Hanwoo. 6, 7, 8 and 9 day embryos produced in vitro were frozen using 1.8M EG+0.1M sucrose, 1.8M EG+0.5% BSA and 1.5M EG+0.1M sucrose media. Survivability was confirmed after frozen-thawed 24 and 48h and ICM, TE cell number were counted by Hoechst 33342 and PI staining after frozen-thawed 24h. As a result, 1.8M EG+0.1M sucrose group was most significantly (p<0.05) higher compared with the other treatment groups on survivability, TE and total cell number after frozen-thawed 24h ($94.2{\pm}2.6%$, $94.67{\pm}3.4$ and $129.67{\pm}5.5$). ICM number did not found significant (p<0.05) differences between the three treatment groups. in 6, 7, 8 and 9 day of embryos using three types of freezing media, frozen-thawed, 1.8M EG+0.1M sucrose groups with embryos cultured 8 day was significantly (p<0.05) highest survivability to $98.3{\pm}1.7%$ after frozen-thawed 24h. 1.5M EG+0.1 sucrose group with embryos cultured 9 day was significantly higher survivability than group of embryos cultured 8 day after frozen-thawed 24 and 48h. In conclusion, 1.8M EG+0.1M sucrose media is considered to be effective to cryopreservation of embryos cultured 8 and 9 day.

• Journal of Radiation Protection and Research
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• v.8 no.2
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• pp.15-35
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• 1983

High voltage paper-electrophoresis of fission products from 24 hour neutron-irradiated and 150 days-decayed 90% highly enriched uranyl nitrate solution has been carried out by using the specially designed migration apparatus. The separation of Zr-95 and Nb-95 from the other fission products is possible under the migration condition of 0.1 $M-HClO_4$ (pH=0.85), 0.05 M-HCl+0.09M-KCl (pH=0.9), 0.1M-HCl (pH=1.1) and 0.01 M-HCl (pH=2.0). Zr-95 and Nb-95 are separated out at+1cm from the fiducial point. The separation of Zr-95 and Nb-95 from each other is possible under the migration condition of 0.1 $M-HClO_4$, 0.05 M-HCl+0.09 M-KCl, 0.1 M-HCl and 0.1 M-HAc+0.1 M-NaAc (pH=4.68) together with 2% ammonium oxalate. Nb-95 is separated out at $-6{\sim}-7cm$ from the fiducial point and Zr-95 at $+1{\sim}-lcm$. The separation of Ru-103 from the other fission products is possible under the migration condition of 0.025 $M-Na_2CO_3+0.025\;M-NaHCO_3$ (pH=10.0), 0.01M-$Na_3PO_4$ (pH=11.7) and 0.1 M-NaOH (pH=13.2). Ru-103 migrates towards the anode -6cm, -4cm and -3cm, respectively.