• Horticultural Science & Technology
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• v.19 no.1
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• pp.81-86
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• 2001

This experiment was carried out to investigate watering with relation to growth, quality and yield of essential oil in sweet basil. The degree of water stress was taken as amount of watering. D1 was watered with 25mL for 2 weeks and 30mL from 4 to 5 weeks after planting in Wagner pot (1/20000a, ${\phi}24cm$). As this, 75mL and 90mL, 225mL and 270mL, and 675mL and 810mL were watered in D2, D3, and D4 treatment respectively. DFT was set up as water stress was not inflicted. The growth of basil in D3 and D4 was better than that of others, in which root activity was as much twice as that of D1. Essential oil of D1 was recorded the lowest content as 0.33%. The result of proline content, peroxidase activity, photosynthesis, stomatal conductance and stomatal resistance were proved D1 to be stressed. This treatment consequently increased the content of essential oil. In consideration of growth and essential oil content, D3 treatment was highest as 47.37mg in oil production per plant. Finally, D3 watered with 225mL for 2 weeks and 275mL from 4 to 5 weeks after planting could be selected on the purpose of both plant growth and essential oil production. Essential oil content of sweet basil was increased in response to water stress. For increase of essential oil yield, oil synthesis could be raised by giving water stress just before harvesting.

• Journal of the Korean institute of surface engineering
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• v.34 no.2
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• pp.105-114
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• 2001

$Al_2$$O_3$ coatings were deposited on M2 high speed steels by the plasma enhanced chemical vapor deposition (PECVD) process, using a gas mixture of AlC1$_3$, $H_2$, $CO_2$ and Ar $Al_2$$O_3$ coatings had interference color and showed amorphous phase. $A1_2$X$A1_3$/($Ti_{0.5}$ /$Al_{0.5}$ )N double layer coatings were produced in the sequence of substrate $NH_3$ plasma pretreatment, ($Ti_{0.5}$$Al_{0.5}$)N depoition process, $Al_2$$O_3$ deposition process. $Al_2$ $O_3$/( $Ti_{0.5}$A $l_{0.5}$)N double layer coatings showed NaCl structure in ( $Ti_{0.5}$A $l_{0.5}$)N layer and amorphous phase in A1$_2$ $O_3$ layer. It was shown that $Al_2$ $O_3$ columns continuously grew onto ( $Ti_{0.5}$A $l_{0.5}$)N columns. ( $Ti_{0.5}$A $l_{0.5}$)N single coating and $Al_2$ $O_3$/( $Ti_{0.5}$A $l_{0.5}$)N double layer coating were oxidized at $700^{\circ}C$, 80$0^{\circ}C$, 90$0^{\circ}C$ for 1hr, 3hr in atmosphere. At 80$0^{\circ}C$, single layer coatings were oxidized, which were examined substrate oxide particle. But $Al_2$ $O_3$/ ( $Ti_{0.5}$A $l_{0.5}$)N double layer coatings maintained the asdeposited state. Therefore, $Al_2$ $O_3$/ ( $Ti_{0.5}$A $l_{0.5}$)N double layer coatings have moreexcellent oxidation resistance than ( $Ti_{0.5}$A $l_{0.5}$)N single layer coatings.X> 0.5/)N single layer coatings.s.

• Journal of Bio-Environment Control
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• v.12 no.3
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• pp.139-146
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• 2003

This study was conducted to investigate the effects on the over-growth inhibition of fruit vegetables (tomato, cucumber, and hot pepper) seedling by the treatment of triazole-typechemicals and to select the most effective chemicals for the over-growth inhibition, and to investigate suitabla concentration treatment and times of the newly selected chemical. The triazole-type chemicals inhibited stem height more, the more the concentration is increased in any crops. The diniconazole showed the highest dwarfing ration, and the tebuconazole and hexaconazole are also good for dwarfing ratio. The diniconazole decreased the plant height of cucumber, tomato, and hot pepper as the concentration is increased. In cucumber, thc dwarfing ratio was 30% in the 5 mg${\cdot}L^{-1}$ treatment, and was 60% in thc l00 mg${\cdot}L^{-1}$ treatment. In tomato and hot pepper, the dwarfing ratio was 30% in the 25 mg${\cdot}L^{-1}$ treatment in any times. In diniconazole treatment, the cucumber and tomato should have 5 mg${\cdot}L^{-1}$ at the lst growth stage and the hot pepper should have 5 mg${\cdot}L^{-1}$ at the 2nd growth stage to obtain good seedlings.

• Korean Journal of Food Science and Technology
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• v.45 no.5
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• pp.583-589
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• 2013

We investigated the delay of kimchi fermentation by the addition of plant extracts. Fifteen plant extracts were screened for inhibitory activity aginst Lactobacillus plantarum by using an agar well diffusion assay, and determined the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) were determined. The lowest MIC for grapefruit seed extract (GFSE; 0.0313 mg/mL) was determined, followed by Caesalpinia sappan L. extract (CSLE; 0.25 mg/mL), and oregano essential oil (OREO; 1.0 mg/mL). GFSE, CSLE, and OREO were individually added to kimchi, and incubated the samples at 10 for up to 20 days. Results showed that the addition of GFSE (0.3 and 0.5%), CSLE (0.1, 0.3, and 0.5%), or OREO (0.5 and 1.0%) led to a significant increase in the pH of kimchi, and also a significant reduction in the numbers of lactic acid bacteria. Taken together, the addition of natural antimicrobial agents can delay kimchi fermentation.

• Bulletin of the Korean Chemical Society
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• v.30 no.2
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• pp.435-440
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• 2009

19 Aromatic ring and L-amino acid ester contained rupestonic acid amide derivatives 2a~2l, 3a~3g were synthesized and preliminarily evaluated in vitro against influenza virus $A_3$,B and herpes simplex virus type 1 (HSV-1), 2(HSV-2) by the national center for drug screening of China. The rusults showed that 2i possessed the highest inhibition against both influenza virus $A_3\;(TC_{50}\;=\;120.6\;{\mu}mol/L,\;IC_{50}=\;19.2\;{\mu}$mol/L, SI = 6.3) and B (T$C_{50}\;=\;120.6\;{\mu}mol/L,\;IC_{50}=\;29.9\;{\mu}$mol/L, SI = 4.0); 2g was more active against influenza $A_3$ virus at very low cytotoxicity ($TC_{50}\;>\;2092.1\;{\mu}mol/L,\;IC_{50}=\;143.7\;{\mu}mol/L,$ SI > 14.6) than the parent compound; Compounds 2b, 2c, 2f showed higher activities both against HSV-1 and HSV-2 than that of the parent compound, and 2f was the most potent inhibitor of HSV-1 ($TC_{50}\;=\;200.0\;{\mu}mol/L,\;IC_{50}\;=\;11.3\;{\mu}mol$/L, SI = 17.7 ) and HSV-2 ($TC_{50}\;=\;200.0\;{\mu}mol/L,\;IC_{50}\;=\;20.7\;{\mu}mol$/L , SI = 9.7).

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.552-552
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• 2016

논의 경우 다양한 영향인자에 의해 비점오염물질이 배출되기 때문에 유출특성을 파악하기가 어렵다. 따라서 본 연구의 목적은 논에서 발생하는 비점오염물질의 유출 특성에 대해 파악하여 추후 효율적인 농업비점오염 관리를 위한 기초자료를 제공하는데 있다. 연구 대상지점으로 춘천시에 위치한 논 1곳(면적: $11,130m^2$)을 선정하였으며. 2014년 5월부터 9월까지 총 11회(9회 유출)의 강우 사상에 대한 오염물질별 오염부하 및 유량가중평균농도(Event Mean Concentration, EMC)를 산정하였다. 모니터링기간 동안 발생된 강우량의 범위는 2.6~95.8 mm로 나타났으며, 선행무강우일수는 0.59~21.2일, 강우강도는 0.33~5.13 mm/hr의 범위로 나타났다. 총 유출량은 $0.16{\sim}497.3m^3$, 유출율은 0.01~0.47(평균 0.24)의 범위로 나타났다. 오염물질별 EMC는 SS 17.1~55.3 mg/L(평균 31.9 mg/L), BOD 1.9~9.7 mg/L(평균 4.2 mg/L), COD 3.6~18.9 mg/L(평균 8.3 mg/L), TOC 3.4~17.4(평균 6.4 mg/L) mg/L, T-N 1.64~7.17 mg/L (평균 3.79 mg/L), T-P 0.16~1.04 mg/L(평균 0.44 mg/L)의 범위로 나타났다. 각 강우사상에 대한 단위면적당 오염부하는 SS 0.005~19.56 kg/ha, $BOD_5$ 0.001~1.70 kg/ha, $COD_{Mn}$ 0.003~3.15 kg/ha, TOC 0.002~1.81 kg/ha, T-N 0.001~0.822 kg/ha, T-P 0.00003~0.115 kg/ha의 범위로 산정되었다. 논의 경우에는 초기유출 발생에 따른 오염부하의 급격한 변화가 없었고, 누적오염부하량비의 그래프도 강우사상의 기울기가 직선에 가깝게 나타났다. 하지만 지속적으로 오염물질을 배출하는 경향과 다양한 조건에 따라 배출양상이 달라지기 때문에 효과적인 관리를 하기 위해서 장기적인 모니터링 연구가 필요할 것으로 사료된다.

• Journal of the Korean Chemical Society
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• v.29 no.6
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• pp.651-655
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• 1985

A series of S-(2,2-diethoxycarbonyl-1-phenylethyl)-L-glutathione derivatives (11a-e) were synthesized from the reaction of ${\beta},\;{\beta}$-diethoxycarbonylstyrene with L-glutathione in 9 : 1 aqueous methanol. Thus, S-(2,2-diethoxycarbonyl-1-phenylethyl)-L-glutathione (11a), S-2,2-diethoxycarbonyl-1-(3',4'-methylenedioxy)phenylethyl-L-glutathione (11b), S-2,2-diethoxycarbonyl-1-(3',4',5'-trimethoxy)phenylethyl-L-glutathione (11c), S-2,2-diethoxycarbonyl-1-(4'-hydroxy)phenylethyl-L-glutathione (11d), S-2,2-diethoxycarbonyl-1-(4'-methoxy)phenylethyl-L-glutathione (11e) were obtained in good yields. The structure of the adducts was characterized by analytical and spectral data. The effects of pH and solvents upon the yields were also briefly examined. In the range of pH from 4.0 to 8.0, the aqueous methanol were found to be the best solvent for the addition reaction and the antibacterial activities of the adducts to Gram(+) bacteria were found to be weak.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.26-39
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• 2020

In maize, immature embryos (IEs) are highly regenerative explants most suitable for producing high frequencies of plantlet regeneration in vitro. Apart from media, explants, and hormones, genotypic variation also influences in vitro characters to a great extent. In the present study, IEs were used to study the distinctive effect of variation of size/stage and hormones in different genotypes on five in vitro characters viz., frequency of callus induction, growth rate of total callus, frequency of E. callus induction, and volume and number of regenerated plantlets. LS medium with different concentrations of 2,4-D (0.5, 1.5, 2.5, 4.0 and 5.0 mg/L) were used to study the former four in vitro characters, and medium with 6-benzylaminopurine and kinetin (0.5 mg/L, each) was used for plantlet regeneration. IEs of 1.0, 1.5, 2.0, 2.5 and 3.0 mm in size were isolated from four inbred lines viz., NM74C, NM81A, NM5883 and NM5884. Two-way ANOVA revealed that explant size and genotypes, as well as hormonal concentrations showed significant effects on in vitro characters. Two millimeter IEs were found to be suitable for in vitro cultures. LS medium with 1.5 mg/L 2,4-D and LS with BAP and Kn (0.5 mg/L, each) were found to be the best hormonal concentrations for callus induction, maintenance, and regeneration, respectively. Among the four genotypes, NM81A and NM5883 yielded more non-embryogenic and Type I E. calli. In contrast, NM74C and NM5884 yielded more highly regenerative Type II calli. Inbred line NM5884 was found to be the best among these four genotypes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.47 no.4
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• pp.279-282
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• 2002

Oryzalexine A, a potent growth inhibitor against several weeds such as Digitaria sanguinalis (L) Scop., and Amaranthus lividus L. was purified by conventional solvent partitioning and column chromatographies. This substance showed strong inhibitory activity on several weeds: Germination of seeds of Poa annua L. was inhibited by 36.5 % at 1.0 mM and Amaranthus lividus L. by 56.1% at 2.5mM. Growth of root and shoot of Digitaria sanguinalis (L.) Scop. by 10.2% and 22.4% at 2.5mM, respectively. These suggest that Oryzalexine A in rice straw might affect the germination and growth of susceptible weeds and other plants.

• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.724-730
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• 2013

We investigated the effects of orally administered probiotic bacteria (Lactobacillus species) as allergic immune modulators in ovalbumin (OVA)-sensitized mice. BALB/c mice were intraperitoneally injected with OVA twice at a 2-week interval for allergy sensitization. The mice were then orally administered Lactobacillus casei YIT9029 (L1), L. casei HY7201 (L2), L. brevis HY7401 (L3), or L. plantarum HY20301 (L4) every 2 days for 3 weeks. Total IgE levels significantly decreased in sera of L3-administered mice but increased in the other groups. OVA-specific IgE levels decreased slightly in sera of mice administered L1, L3, and L4 but increased significantly in L2-administered mice. In passive cutaneous anaphylaxis (PCA) using sera from administered mice, only the L3-administered group showed reaction inhibition. High expression of TLR-2 with interferon (IFN)-${\gamma}$ stimulation on peripheral blood mononuclear cells occurred in L3- or L4-administered mice. Th1 cytokines, including IFN-${\gamma}$ and interleukin (IL)-12, increased in splenocytes of L3-administered mice; however, IL-4 decreased in L1- and L4-administered groups; IL-5 decreased in all experimental groups. IL-6 decreased in the L3-administered group; and IL-10 decreased in L1-, L2-, and L3-administered groups. L3 induced antiallergic effects by increasing Th1 cytokines, decreasing Th2 cytokines, and inhibiting the PCA reaction, whereas L2 administration increased allergic effects.