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Studies on the Flowering and Maturity in Sesame (Sesamum indicum L.) III. Growth of Capsule and Grain by Different Plant Types (참깨의 개화.등숙에 관한 연구 III. 참깨 초형별 삭과 및 종실의 발육)

  • Kang, Chul-Whan;Lee, Jung-Il;Son, Eung-Ryong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.2
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    • pp.158-164
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    • 1985
  • The objective of the study was to investigate growth pattern of capsule and grain to improve grain filling during the grain filling period in sesame. Growth patterns of capsule and grain from anthesis to maturity were measured and compared by different plant types. Growth of capsule length started to grow just after anthesis and recorded maximum point at 35 days after flowering. and then decreased gradually. Growth of higher part capsule was worse than lower and middle capsules. Capsule growth of 2 carpels 4 loculi type showed better than 4 carpels 8 loculi type and BTB (branch, 3 capsules, 2 carpels, 4 loculi) type showed good growth due to its small reduction of higher part capsule length compared to those of lower and middle parts. The order of growth of capsule length were considered to be center capsule in main stem> center capsule in branch> side capsule in main stem> side capsule in branch. Growth of capsule width also showed maximum at 35 days after anthesis and then reduced. The order of growth of capsule width were lower part> middle part> higher part. Higher part capsule width of 3 capsules 4 carpels 8 loculi type showed serious decrease at late reproductive growth stage same as those of capsule length. Fresh one thousand grain weight showed peak at 35 days after anthesis and then reduced. The order of grain growth were appeared as lower part> middle part> higher part. Growth of fresh one thousand grain weight of branch and side capsule were lower than those of main stem center capsule, and 4 carpels 8 loculi type was deeply decreased at late flowering time in higher part such as the growth of capsule length and width. BTB (branch, 3,2/4) type didn't show much decrease in higher part fresh grain weight compared to those of other plant types in spite of its profitable character of lots of sink capacity. BTB type appeared to be ideal for improving grain filling and yield productivity in growing sesame in Korea.

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Optimization of the Reaction Conditions and the Effect of Surfactants on the Kinetic Resolution of [R,S]-Naoroxen 2,2,2-Trifluoroethyl Thioester by Using Lipse (리파아제를 이용한 라세믹 나프록센 2,2,2-트리플로로에틸 씨오에스터의 Kinetic Resolution에서 반응조건 죄적화와 계면활성제 영향)

  • Song, Yoon-Seok;Lee, Jung-Ho;Cho, Sang-Won;Kang, Seong-Woo;Kim, Seung-Wook
    • KSBB Journal
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    • v.23 no.3
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    • pp.257-262
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    • 2008
  • In this study, the reaction conditions for lipase-catalyzed resolution of racemic naproxen 2,2,2-trilfluoroethyl thioester were optimized, and the effect of surfactants was investigated. Among the organic solvents tested, the isooctane showed the highest conversion (92.19%) in a hydrolytic reaction of (S)-naproxen 2,2,2-trifluoroethyl thioester. In addition, the isooctane induced the highest initial reaction rate of (S)-naproxen 2,2,2-trifluoroethyl thioester ($V_s=2.34{\times}10^{-2}mM/h$), the highest enantioselectivity (E = 36.12) and the highest specific activity ($V_s/(E_t)=7.80{\times}10^{-4}mmol/h{\cdot}g$) of lipase. Furthermore, reaction conditions such as temperature, concentration of the substrate and enzyme, and agitation speed were optimized using response surface methodology (RSM), and the statistical analysis indicated that the optimal conditions were $48.2^{\circ}C$, 3.51 mM, 30.11 mg/mL and 180 rpm, respectively. When the optimal reaction conditions were used, the conversion of (S)-naproxen 2,2,2-trifluoroethyl thioester was 96.5%, which is similar to the conversion (94.6%) that was predicted by the model. After optimization of reaction conditions, the initial reaction rate, lipase specific activity and conversion of (S)-naproxen 2,2,2-trifluoroethyl thioester increased by approximately 19.54%, 19.12% and 4.05%, respectively. The effect of surfactants such as Triton X-100 and NP-10 was also studied and NP-10 showed the highest conversion (89.43%), final reaction rate of (S)-naproxen 2,2,2-trifluoroethyl thioester ($V_s=1.175{\times}10^{-2}mM/h$) and enantioselectivity (E = 59.24) of lipase.

Increase in Anti-Oxidant Components and Reduction of Off-Flavors on Radish Leaf Extracts by Extrusion Process (압출성형 무청 분말 추출물의 항산화 물질 함량 증가 및 이취 감소)

  • Sung, Nak-Yun;Park, Woo-Young;Kim, Yi-Eun;Cho, Eun-Ji;Song, Hayeon;Jun, Hyeong-Kwang;Park, Jae-Nam;Kim, Mi-Hwan;Ryu, Gi-Hyung;Byun, Eui-Hong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.12
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    • pp.1769-1775
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    • 2016
  • Aerial parts (leaves and stems) of radish are usually discarded due to the distinct undesirable flavors associated with inappropriate preparations, despite their many health benefits. In this study, we examined the role of extrusion process in the removal of off-flavors and elevation of antioxidant activity in radish (Raphanus sativus L.) leaves and stems. To optimize the extrusion conditions, we changed the barrel temperature (110, 120, and $130^{\circ}C$), screw speed (150, 200, 250, and 300 rpm), and moisture content (20, 25, and 30%). The polyphenol and flavonoid contents significantly increased in extruded radish leaves and stems (ER) under optimum extrusion conditions ($130^{\circ}C$, 250 rpm, and 20%). Under extrusion conditions, we compared off-flavors (as amount of sulfur-containing compound) levels between ER and non-extruded radish leaves and stems (NER) by an electronic nose. A total of six peaks (sulfur-containing compound) were similarly detected in both ER and NER, whereas the ER showed reduced off-flavors. Levels of glucosinolate (${\mu}g/g$), which can be hydrolyzed into off-flavors during mastication or processing, were significantly decreased in the ER. From these results, extrusion processing can be an effective method to increase anti-oxidant activity and removal of off-flavors in radish leaves and stems.

Ecological Studies on the Epilithic Algal and Change of Agricultural Irrigation Water Quality in the Seomjin River (섬진강 수계 수질과 부착조류에 대한 생태학적 연구)

  • Lee, Kyeong-Bo;Kim, Baik-Ho;Lee, Deog-Bae;Kim, Jae-Duk;Choi, Min-Kyu
    • Korean Journal of Environmental Agriculture
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    • v.19 no.4
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    • pp.276-283
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    • 2000
  • This study was carried out to investigate distribution of epilithic algal and chemical contents of irrigation water influencing in the Seomjin River from March to October in 1996 and 1997. In the water quality of the Seomjin River, the monthly average contents of $NH^+\;_4-N,\;SO_4\;^{2-}$ and $Cl^-$ showed the lowest peak in July and August while that of $NO_3-N$ showed the highest peak in June. The water quality of the Seomjin River was deteriorated by inflow of Yo-cheon and Sosi-cheon in the middle stream. The concentration of anions and cations of the Seomjin River was $Cl^->SO_4\;^{2-}>NO_3\;^->PO_4\;^{3-}\;and\;Ca^{2+}>Na^+>Mg^{2+}>K^+>NH_4\;^+$, respectively. The epilithic algal community identified 339 taxa consisting of 7 forma, 42 varieties and 290 species in 6 classes. In species composition, green algae and diatoms were heavily dominated which were evaluated 50.4% and 31.3% of total classification, respectively. In seasonal distribution, 219 species presented in summer but, 147 species were in winter. In this study, dominant species were Cocconeis placentula var. euglypta and Cosmarium furcatospermum in spering. Cyclotella meneghinasa and Synedra ulna, Scenedesmus ecornis in summer. Melosira varians, Achnathes minutissima in autumn and Gomphonema parvulum in winter were dominated. Finally, the dominant indices in this study have ranged 0.10-0.43.

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Bio-Degradable Plastic Mulching in Sweetpotato Cultivation (생분해성 멀칭필름을 이용한 고구마 재배)

  • Lee, Joon-Seol;Jeong, Kwang-Ho;Kim, Hag-Sin;Kim, Jeong-Ju;Song, Yeon-Sang;Bang, Jin-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.2
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    • pp.135-142
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    • 2009
  • This experiment was conducted to determine the usability of biodegradable plastic in the mulching cultivation of sweetpotato. For this, we investigated the physical characteristics, biodegradability, leaching, yield, workability, etc. of biodegradable films. Compared with general mulching materials, biodegradable Poly butyleneadipate-co-butylene succinate (PBSA) and PLC+starch showed $2{\sim}27$% higher tensile strength, but $2{\sim}22$% lower elongation and $2{\sim}6$% lower tear strength. In the leaching test on the biodegradable films, heavy metals were detected very little or not at all. As to difference in ground temperature according to mulching material, the temperature was high in order of PLC+starch > PBSA > Low Density Polyethylene (LDPE) > Control during the period from late June to mid July, but in order of LDPE > PLC+starch > PBSA > None during the period from late July to late September. In the mulching cultivation of sweet potato, biodegradable films PBSA (EA, EB, EC) and PLC+starch (DD, DE, DF) began to degrade after 60 days from the cut planting of sweet potato, and over 95% degraded after 120 days. The quantity of roots was 3,070 kg/10a for PBSA, 3,093 kg/10a for PLC-starch, and 2,946 kg/l10a for LDPE, showing no significant difference according to mulching material. Considering the physical characteristics, biodegradability, environment, convenience in harvesting work, yield, etc. of the films in the mulching cultivation of sweet potato, biodegradable films are expected to be very useful.

Spatio-temporal Water Quality Variations at Various Streams of Han-River Watershed and Empirical Models of Serial Impoundment Reservoirs (한강수계 하천에서의 시공간적 수질변화 특성 및 연속적 인공댐호의 경험적 모델)

  • Jeon, Hye-Won;Choi, Ji-Woong;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.45 no.4
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    • pp.378-391
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    • 2012
  • The objective of this study was to determine temporal patterns and longitudinal gradients of water chemistry at eight artificial reservoirs and ten streams within the Han-River watershed along the main axis of the headwaters to the downstreams during 2009~2010. Also, we evaluated chemical relations and their variations among major trophic variables such as total nitrogen (TN), total phosphorus (TP), and chlorophyll-a (CHL-a) and determined intense summer monsoon and annual precipitation effects on algal growth using empirical regression model. Stream water quality of TN, TP, and other parameters degradated toward the downstreams, and especially was largely impacted by point-sources of wastewater disposal plants near Jungrang Stream. In contrast, summer river runoff and rainwater improved the stream water quality of TP, TN, and ionic contents, measured as conductivity (EC) in the downstream reach. Empirical linear regression models of log-transformed CHL-a against log-transformed TN, TP, and TN : TP mass ratios in five reservoirs indicated that the variation of TP accounted 33.8% ($R^2$=0.338, p<0.001, slope=0.710) in the variation of CHL and the variation of TN accounted only 21.4% ($R^2$=0.214, p<0.001) in the CHL-a. Overall, our study suggests that, primary productions, estimated as CHL-a, were more determined by ambient phosphorus loading rather than nitrogen in the lentic systems of artificial reservoirs, and the stream water quality as lotic ecosystems were more influenced by a point-source locations of tributary streams and intense seasonal rainfall rather than a presence of artificial dam reservoirs along the main axis of the watershed.

Clinical and Bacteriologic Efficacy of Cefdinir on Pharyngitis and Pharyngotonsillitis Caused by Group A Beta Hemolytic Streptococci in Children (Group A-beta Hemolytic Streptococci에 의한 소아 인두편도염에 있어서 Cefdinir의 항균력과 임상적 및 세균학적 효과에 관한 연구)

  • Chung, Ji-Young;Sin, Seon-Hee;Ahn, Young-Min;Ahn, Byung-Moon;Sin, Young-Gyu;Bae, Young-Min;Park, Soo-Eun;Kim, Jong-Guk;Lee, Jong-Guk;Ma, Saung-Hyuk;Chang, Jin-Kun;Cha, Sung-Ho
    • Pediatric Infection and Vaccine
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    • v.10 no.1
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    • pp.95-101
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    • 2003
  • Objective : To determine the clinical and bacteriologic efficacy and safety of Cefdinir in acute pharyngitis and pharyngotonsillitis caused by group A beta hemolytic streptococci in pediatric patients. Methods : Children aged 3 through 12 years who visited the hospitals enrolled in this study with signs and symptoms of pharyngitis or pharyngotonsillitis since May to December 2002, were taken throat culture and given Cefdinir(12 mg/kg/day, in three divided doses) for 7 days. Two hundred thirty five patients were enrolled and 90 patients who showed positive culture results were followed up for the signs and symptoms during the treatment to determine clinical efficacy. Follow-up culture were done at the end of the study and bacteriologic efficacy was determined Results : Ninety out of 235 patients who visited the hospitals with the signs and symptoms of pharyngitis showed positive growth on throat culture. Seventy nine patients were clinically and microbiologically assessable. The bacteriologic eradication rates of S. pyogenes were 100% in all the children treated with Cefdinir. Clinical cure rates were not different between less than 7 days-treated group(75%) and just 7 days-treated group(98.6%)(P=0.077). Two patients reported adverse reaction during Cefdinir treatment. Antimicrobial sensitivity of Cefdinir against S. pyogenes was 100% with range of MIC being less than 0.5 ${\mu}g/mL$. Conclusion : It seems that Cefdinir is one of reliable and well-tolerated drugs for the treatment of group A beta hemolytic streptococcal pharyngotonsillitis in children. It needs to be investigated short term efficacy in terms of improving drug compliance and impact of economic point of view. We can recognized that still high rate of the erythromycin resistant group A streptococci in our community should be monitored every year.

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Simultaneous Determination of Carbohydrates and Organic Acids in Various Cultured Dairy Foods by High-Performance Liquid Chromatography: A Preliminary Study (다양한 낙농 발효유제품에서 HPLC를 이용하여 탄수화물과 유기산의 동시 검출에 관한 연구)

  • Kim, Dong-Hyeon;Hwang, Dae-Geun;Chon, Jung-Whan;Kim, Hyunsook;Kim, Hong-Seok;Song, Kwang-Young;Yim, Jin-Hyuk;Kim, Young-Ji;Kang, Il-Byung;Lee, Soo-Kyung;Seo, Kun-Ho
    • Journal of Dairy Science and Biotechnology
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    • v.33 no.4
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    • pp.263-269
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    • 2015
  • Various carbohydrates (lactose, glucose, and fructose), lactic acid, uric acid, and acetoin were separated on a ZORBAX Carbohydrate Analysis column using the Agilent 1200 HPLC ChemStation$^{TM}$, and were identified according to retention times with 325 Dual Wavelength UV-Vis Detector and Refractive Index Detector with 0.013 N $H_2SO_4$ at a flow rate of 0.8 mL/min. In addition, the lactase activity of four commercial probiotic lactic acid bacteria during 6-hour incubation was determined using a high-performance liquid chromatography (HPLC) method. Among the tested samples, Bifidobacterium animalis subsp. lactis showed the greatest lactase activity, followed by Lactobacillus rhamnosus and Lactobacillus casei, with Streptococcus salivarius subsp. thermophilus showing the lowest activity. Therefore, this HPLC technique shows potential for evaluating the fermentation processes of probiotic lactic acid bacteria and could simultaneously confirm the degree of ripening in various fermented dairy foods within only a half hour.

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Effect of Gamma Irradiation and Cichorium Products on Oxidative Damage and Lipid Metabolism in Streptozotocin-Induced Diabetic Rats (감마선 전신 조사와 치커리 가공물 식이가 Streptozotocin 유발 당뇨쥐의 산화적 손상과 지질대사에 미치는 영향)

  • Woo, Hyun-Jung;Kim, Ji-Hyang;Kim, Jin-Kyu;Kim, Hee-Jung;Park, Ki-Beom
    • Korean Journal of Environmental Biology
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    • v.24 no.2 s.62
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    • pp.102-111
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    • 2006
  • The increased occurrence of hyperglycemia and oxidative stress in streptozotocin (STZ) induced type I diabetes has been implicated in the etiology and pathology of disease complication. STZ has known to be genotoxic in a variety of assays including tests for microbial mutagenesis and unscheduled DNA synthesis in rat kidney. Diabetes mellitus (DM) is a pathologic condition, resulting in severe metabolic imbalances and non-physiologic changes in many tissues. We examined the effect of gamma radiation and KWNP on preventing the development of insulin dependent diabetes mellitus using streptozotocin-induced Fisher 344 diabetic rats. The hematological values (red blood cell and white blood cell), serum biochemical constituents-alkaline phosphatase (ALP), total cholesterol, triglycerides and insulin-were checked and the organs (testis, spleen and kidney) were weighed. The gonad indices of the STZ treated groups were much lower than the value of the control group. But the gonad indices of the KWNP treated groups were higher than those of the treated groups. The ratio of the weight of kidney to the body weight of the STZ treated groups was higher than that of the control group. The value of the diabetic group treated with KWNP after irradiation (F group) was lower than the other STZ treated groups. The white blood cell and ALP values of the F group were lower than the other STZ groups, as well. The cholesterol and triglyceride values of all the KWNP treated groups were significantly lower than the other groups. A significant increase (about 10 times) of insulin was detected in the F group. The results of hematological assay showed the distinctive damage in the irradiated and STZ treated groups. The quantity of apoptotic cells in seminiferous tubule of testis confirmed a serious damage as assessed in the STZ treated groups. These experimental results have revealed that treatment of the products of KWNP after irradiation has the antidiabetic effect in the STZ-induced diabetic rats. But the F group showed higher recuperative power. These experimental results have revealed that treatment of the gamma irradiation and KWNP have the recovering effect in the STZ-induced diabetic rats.

Effect of Maturity at Harvest and Inoculants on the Quality of Round Baled Rye Silage (수확시 숙기 및 젖산균 제제가 호밀 라운드베일 사일리지의 품질에 미치는 영향)

  • Kim, J.G.;Kim, D.A.;Chung, E.S.;Kang, W.S.;Ham, J.S.;Seo, s.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.4
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    • pp.347-354
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    • 1999
  • This experiment was carried out to determine the effect of maturity at harvest and inoculants on the quality of round baled rye(Secale cereale L.) silage at the experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon in 1998. The experiment was consist of split-plot design with 3 replications. The main plots were 3 harvesting stages such as boot(20 April), heading(29 April), and flowering stages(14 May). The subplots wered inoculant treatments : control (untreated), inoculant A, and inoculant B. Acid detergent fiber(ADF), neutral detergent fiber(NDF), and in vitro dry matter digestibility (IVDMD) of rye silage were significantly increased with delayed harvesting date, but there was not significant difference between inoculants. Mean silage pH at flowering stage was the lowest(4.35), but the highest at early harvest(4.91). Inoculants significantly reduced acidity of silage compared with control. Dry matter(DM) content of the control was higher than that of inoculants. Ammonia-N as proportion of total N was below 10% which was maximum level of high quality silage. The addition of inoculants reduced ammonia-N. There were significant difference in organic acid contents between harvesting stages and inoculants. Lactic acid was increased with inoculants, but acetic and butyric acids were decreased. Various treatments increased colony forming unit(CFU) of lactic acid bacteria by 2 or 3 times compared with the control and the highest at flowering stage with inoculant B treatment. Results of this study indicate that use of microbial inoculant and harvesting after heading stage will improve the silage fermentation and quality of round baled rye silage.

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