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Control Standards of Three Major Insect Pests of Chinese Cabbage (Brassica campestris) Using Drones for Pesticide Application (농약살포용 드론을 이용한 배추 주요해충 3종의 방제기준 설정)

  • Choi, Duck-Soo;Ma, Kyung-Cheol;Kim, Hyo-Jeong;Lee, Jin-Hee;Oh, Sang-A;Kim, Seon-Gon
    • Korean journal of applied entomology
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    • v.57 no.4
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    • pp.347-354
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    • 2018
  • In order to setting the control standard of Chinese cabbage pests using a drone, the downward wind speed, spraying width, and the number of falling particles and particle size were examined using a water sensitive paper with spray different heights (3, 4, 5 m) and flying speeds (3, 4 m/sec). Fore kinds of pesticides for aviation control were used to test the perfect lethal concentration and dose for major pests of Chinese cabbage such as Plutella xylostella, Spodoptera exigua and Spodoptera litura. The number of falling particles in spraying pesticides with drones was 80.5% on the upper side, 14.8% on the vertical side, and 4.7% on the back side. The number of falling particles as different spray heights were 3 m = 53, 4 m = 40 and $5m=39particles\;cm^{-2}$. The number of falling particles as different flying speeds were $3m\;sec^{-1}=62$ and $4m\;sec^{-1}=25particles\;cm^{-2}$. In the laboratory test, the perfect lethal concentration and dose of Plutella xylostella was chlorfenapyr SC (20 times, $0.5{\mu}l$) and bistrifluron chlorfenapyr SC (25 times, $0.5{\mu}l$). The perfect lethal concentration and dose of Spodoptera exigua was chlorfenapyr SC (20 times, $1{\mu}l$), bistrifluron chlorfenapyr SC (20 times, $1{\mu}l$), and chlorfenapyr SC (20 times, $1{\mu}l$) and bistrifluron chlorfenapyr SC (20 times, $0.5{\mu}l$) for Spodoptera litura. Therefore, the main pest control method of Chinese cabbage using drones is 20 times diluted chlorphenapyr SC or bistrifluoruron-chlorphenapyr SC, sprayed at 3 m height by $3msec^{-1}$ of going speed. This spraying method will be effective for control of Chinese cabbage pest.

Validity of Paramedian Tangential Approach to L5-S1 Far-Lateral Lesions

  • Baek, Seung-Jin;Kim, Joo-Seung;Moon, Byung-Gwan;Lee, Seung-Jin;Kang, Hee-In
    • Journal of Korean Neurosurgical Society
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    • v.39 no.5
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    • pp.366-369
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    • 2006
  • Objective : There are various surgical approaches to far-lateral lesions in the L5-S1 intervertebral space. Of these is the validity of a paramedian tangential approach is being investigated in this study. Methods : A retrospective study was conducted on 25 patients who had been diagnosed as having a far-lateral L5-S1 disc herniations, osteophyte, costal process hypertrophy, and had undergone a paramedian tangential approach from November 1999 through December 2003. The degree of symptoms and improvement were compared via the visual analog pain scale, before and after surgery. Results : This study included 4 males and 21 females with a mean age of $62{\pm}11.8$ years old. The average follow-up period after surgery was $8.2{\pm}2.7$ months. The visual analog pain scale taken before surgery was $6.7{\pm}1.1$ points, while the post-surgical scale was $2.4{\pm}0.9$ points showing a significant degrease [p < 005]. There were no complications that developed during surgery. Conclusion : A paramedian tangential approach is less invasive in the soft tissue than that of the median approach. This approach may effectively reduce nerve root compression and expand intervertebral foramens, and is devoid of the risk of spinal instability after surgery. The authors suppose that a paramedian tangential approach is quite an effective technique to relive compression in the far-lateral L5-S1 intervertebral space.

Tyrosinase Inhibitors Isolated from the Edible Brown Alga Ecklonia stolonifera

  • Kang, Hye-Sook;Kim, Hyung-Rak;Byun, Dae-Seok;Son, Byeng-Wha;Nam, Taek-Jeong;Choi , Jae-Sue
    • Archives of Pharmacal Research
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    • v.27 no.12
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    • pp.1226-1232
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    • 2004
  • Extracts from seventeen seaweeds were determined for tyrosinase inhibitory activity using mushroom tyrosinase with L-tyrosine as a substrate. Only one of them, Ecklonia stolonifera OKAMURA (Laminariaceae) belonging to brown algae, showed high tyrosinase inhibitory activity. Bioassay-guided fractionation of the active ethyl acetate (EtOAc) soluble fraction from the methanolic extract of E. stolonifera, led us to the isolation of phloroglucinol derivatives [phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5)]. Compounds 1~5 were found to inhibit the oxidation of L-tyrosine catalyzed by mushroom tyrosinase with $IC_{50}$ values of 92.8, 126, 33.2, 177, and 2.16 ${\mu}g$ /mL, respectively. It was compared with those of kojic acid and arbutin, well-known tyrosinase inhibitors, with $IC_{50}$ values of 6.32 and 112 ${\mu}g$ / mL, respectively. The inhibitory kinetics analyzed from Lineweaver-Burk plots, showed compounds 1 and 2 to be competitive inhibitors with $K_i$ of $2.3{\times}10^{-4}\;and\;3.1{times}10^{-4}$ M, and compounds 3~5 to be noncompetitive inhibitors with $K_i$ of $1.9{\times}10^{-5},\;1.4{\times}10^{-3}\;and\;1.5{\times}10^{-5}$ M, respectively. This work showed that phloroglucinol derivatives, natural compounds found in brown algae, could be involved in the control of pigmentation in plants and other organisms through inhibition of tyrosinase activity using L-tyrosine as a substrate.

Influence of Pre-operative Fasting Time on Blood Glucose in Older Patients (수술 전 금식이 노인환자의 혈당에 미치는 영향)

  • Hong, Mi-Suk;Yoon, Hae-Sang
    • Journal of Korean Academy of Nursing
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    • v.41 no.2
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    • pp.157-164
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    • 2011
  • Purpose: This study was performed to identify changes in blood glucose at preoperative fasting time in surgical patients over 60 yr. Methods: Data collection was performed from July, 2008 through July, 2009. Participants consisted of 80 nondiabetic surgical patients. Blood glucose was checked from 3 to 5 times. The 5 times were 2-hr fasting on the pre-operative day (T1, n=80), 8 hr (T2, n=80), 10 hr (T3, n=17), 12 hr (T4, n=34) and 14 hr fasting on the day of the operation (T5, n=29). Results: Of the patients, 27.5% had a blood glucose level of less than 79 mg/dL at T2; 17.6% at T3; 32.4% at T4; and 17.2% at T5. Mean blood glucose levels were 93.8 mg/dL at T1; 88.4 mg/dL at T2; 91.7 mg/dL at T3; 87.4 mg/dL at T4: and 94.1 mg/dL at T5. Blood glucose was the lowest at T2 (p<.001). Conclusion: As 17.6-32.4% of the patients showed the blood glucose level of less than 79 mg/dL at 8-14 hr pre-operative fasting, the authors recommend that surgical patients p>60 yr-of-age be observed for hypoglycemia during pre-operative fasting of more than 10 hr and that surgical patients >60 yr-of-age with risks for hypoglycemia be scheduled for operation within 10 hr preoperative fasting.

Plantlet Regeneration by Tissue Cultures of Cyclamen persicum Mill. (시클라멘 (Cyclamen persicum Mill.)의 조직배양에 의한 식물체 재분화)

  • 은종선;김영선;한상권
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.6
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    • pp.479-484
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    • 2000
  • This study was conducted to examine the effects of explant sources and plant growth regulators on mass propagation of Cyclamen persicum. Tuber, cotyledon, and petiole tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Shoots were not induced from calli on cotyledon and petiole explants cultured on MS medium containing various concentrations of 2,4-D or NAA. However, multiple shoots were formed directly from tuber explants cultured on the medium containing 0.5 and 1.0 mg/L 2,4-D or NAA. In MS medium with cytokinin alone, TDZ was more effective in shoot formation than BA or kinetin in all explants. The combinations of NAA and BA was found to be most effective in shoot formation from tuber, cotyledon and petiole explants. Especially, shoots were formed from all the tuber explants on the medium containing 0.5 mg/L of NAA and BA. Hormonal effects on root formation were examined by subculturing single shoots on MS medium containing NAA or IBA. The medium with 0.5 mg/L IBA was most effective in root induction and subsequent plantlet regeneration.

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Symptoms, Mental Health, and Quality of Life Among Patients After COVID-19 Infection: A Cross-sectional Study in Vietnam

  • Hai Nguyen Thanh;Duc Cap Minh;Hien Hoang Thu;Duc Nguyen Quang
    • Journal of Preventive Medicine and Public Health
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    • v.57 no.2
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    • pp.128-137
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    • 2024
  • Objectives: This study was conducted to characterize the symptoms, mental health, quality of life (QoL), and associated factors following the coronavirus disease 2019 (COVID-19) infection. Methods: This cross-sectional study included 394 participants previously infected with COVID-19 in Ho Chi Minh City, Vietnam. Mental health was assessed using the 21-item Depression, Anxiety, and Stress Scale (DASS-21). Participants self-reported health-related QoL was measured with the EuroQol 5-Dimension 5-Level (EQ-5D-5L) scale. Results: Among the participants, 76.4% reported experiencing at least one symptom following COVID-19 infection. The most common symptoms were fatigue (42.1%), cognitive dysfunction (42.9%), and hair loss (27.9%). According to the DASS-21 results, the proportions of depression, anxiety, and stress were 28.7%, 26.4%, and 20.6%, respectively. The mean scores on the EQ-5D-5L and the EuroQol Visual Analog Scale were 0.94±0.11 and 84.20±13.11, respectively. Regarding QoL issues, the highest proportion of participants (32.7%) reported experiencing anxiety or depression, followed by pain or discomfort (25.4%). Multivariable logistic regression analysis revealed that factors associated with the presence of symptoms following COVID-19 infection included female (odds ratio [OR], 2.84; 95% confidence interval [CI], 1.65 to 4.91) and having QoL issues (OR, 3.25; 95% CI, 1.71 to 6.19). Conclusions: The study investigated the prevalence rates of various symptoms following COVID-19 infection. These findings underscore the need to prioritize comprehensive care for individuals recovering from COVID-19 and to implement strategies to mitigate the long-term impact of the disease on mental health and QoL.

Efficient Sewage Treatment with Less sludge Production by Used Briquets (연탄재를 이용한 슬러지 저감 하수처리 연구)

  • Jeong, Doo-Young;Jeong, Myung-Hee;Song, Dae-Yong;Kim, Young-Jun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.16 no.2
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    • pp.81-87
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    • 2008
  • We have investigated the efficacy and efficiency of the used briquets for the sewage treatment in this study. The effluent of wastewater treated by Used Briquets Process (UBP) showed very low values of $BOD_5$, CODcr, and SS (1.1 mg/L, 9.5 mg/L, and 3.8mg/L, respectively), which corresponded to 99%, 92.7%, and 96.3% of removal efficiency to the raw wastewater. The number of total bacteria and E. coli in the effluent water treated by UBP was found to be 9 and 1.2 cfu/ml, respectively, showing the remarkable decrease in number, when compared to that of other sewage treatment plants. In the long period of experiment over 1 year, UBP has also shown the high removal efficiency with less sludge production, and low seasonal fluctuation of the values. This UBP is very environmentally-friendly process in the aspects of recycling used briquets and reducing the sludge production. We think that this process will be useful for both the small-scale sewage treatment and reuse of the effluent water.

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Ethanol Production with Glucose/Xylose Mixture by Immobilized Pichia stipitis (고정화 Pichia stipitis 를 이용한 글루코오스/자일로오스 혼합당으로부터 에탄올 생산)

  • Shin, Hyun-Seok;Kang, Seong-Woo;Lee, Sang-Jun;Jang, Eun-Ji;Suh, Young-Woong;Kim, Seung-Wook
    • KSBB Journal
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    • v.25 no.4
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    • pp.351-356
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    • 2010
  • To increase the production of ethanol by using sugar from lignocellulosic biomass, pentose and hexose have to be fermented simultaneously by yeast. The effects of mixed sugar and nitrogen on ethanol production by immobilized Pichia stipitis KCCM 12009 were investigated. When optimal mixed sugar and nitrogen concentration were 5% (Glucose/Xylose = 3:1) and 1%, respectively, ethanol concentration produced by immobilized P. stipitis was 19-20 g/L. In repeated fed-batch by immobilized P. stipitis, all glucose was consumed very quickly at 1-3% mixed sugar concentration. But, xylose consumption was decreased as the mixed sugar concentration increased. Also, ethanol (5.6 g/L) was stably produced and ethanol production rate was 0.13 g/$L{\cdot}h$ in immobilized cell reactor (ICR) with 1% mixed sugar (Glucose/Xylose = 3:1) as feeding media.

Decreased Voltage Dependent $K^+$ Currents in Cerebral Arterial Smooth Muscle Cells of One-Kidney, One-Clip Goldblatt Hypertensive Rat

  • Oh, Young-Sun;Kim, Se-Hoon;Kim, Hoe-Suk;Jeon, Byeong-Hwa;Chang, Seok-Jong;Kim, Kwang-Jin
    • The Korean Journal of Physiology and Pharmacology
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    • v.3 no.5
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    • pp.471-479
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    • 1999
  • The Kv channel activity in vascular smooth muscle cell plays an important role in the regulation of membrane potential and blood vessel tone. It was postulated that increased blood vessel tone in hypertension was associated with alteration of Kv channel and membrane potential. Therefore, using whole cell mode of patch-clamp technique, the membrane potential and the 4-AP-sensitive Kv current in cerebral arterial smooth muscle cells were compared between normotensive rat and one-kidney, one-clip Goldblatt hypertensive rat (lK,lC-GBH rat). Cell capacitance of hypertensive rat was similar to that of normotensive rat. Cell capacitance of normotensive rat and 1K,lC-GBH rat were $20.8{\pm}2.3$ and $19.5{\pm}1.4$ pF, respectively. The resting membrane potentials measured in current clamp mode from normotensive rat and 1K,lC-GBH rat were $-45.9{\pm}1.7$ and $-38.5{\pm}1.6$ mV, respectively. 4-AP (5 mM) caused the resting membrane potential hypopolarize but charybdotoxin $(0.1\;{\mu}M)$ did not cause any change of membrane potential. Component of 4-AP-sensitive Kv current was smaller in 1K,lC-GBH rat than in normotensive rat. The voltage dependence of steady-state activation and inactivation of Kv channel determined by using double-pulse protocol showed no significant difference. These results suggest that 4-AP-sensitive Kv channels playa major role in the regulation of membrane potential in cerebral arterial smooth muscle cells and alterations of 4-AP-sensitive Kv channels would contribute to hypopolarization of membrane potential in 1K,lC-GBH rat.

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Effects of Dietary Feldspar on Growth Performance and Body Composition in Broiler Chicks (장석의 첨가가 육계의 성장 및 체조성에 미치는 영향)

  • Ko J.W.;Uuganbayar D.;Yang C.J.
    • Korean Journal of Poultry Science
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    • v.33 no.2
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    • pp.105-111
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    • 2006
  • This experiment was conducted to evaluate the effects of feldspar on growth performance and body composition in broiler chicks. A total of 120 one-day old 'Ross' broilers were assigned to 5 treatments in a completely randomized design. The five dietary treatments were control, antibiotic(0.05% chlortetracycline), feldspar 0.5, 1.0, 1.5% diets. The weight gain of broilers fed a diet containing 1.5% feldspar (2,088 g) was significantly (P<0.05) higher than control (1,888 g). The feed intake was significantly increased in feldspar treatments compared to that of the control (P<0.05). However, feed conversion ratio was significantly increased in 1.0% feldspar treatment compared to that of the control (P<0.05). The moisture and protein contents of meat were significantly increased in 1.0% feldspar treatment (75.69 and 15.22%) compared to that of the control (73.38 and 9.80%). However, the crude fat content of meat was reduced in feldspar treatments compared to control and antibiotics treatments (P<0.05). The TBA value of meat in feldspar treatments was significantly reduced compared to that of the control (P<0.05). The plasma cholesterol content was 129 mg/dL in 0.5% feldspar and 134 mg/dL in 1.0% feldspar treatments, which were significantly higher (P<0.05) than that of the control (103 mg/dL).