• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.766-771
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• 2004

Julong 601, a Gram-positive anaerobic bacterium strain capable of cleaving the C-ring of isoflavones daidzein and genistein, was isolated from human feces. BLAST search revealed that its complete 16S rDNA gene sequence has 99% similarity to Eubacterium ramulus. Metabolites of daidzein and genistein were determined as O-desmethylangolensin (O-Dma) and 2-(4-hydroxyphenyl) propionic acid (2-HPPA), respectively, based on UV, EI-MS, and $^1H$ and ^{13}C$ NMR spectral analyses. Enantiomers of O-Dma and 2-HPPA were isolated by chiral stationary-phase HPLC (CSP HPLC). Cleavage of the C-ring of daidzein and genistein by strain Julong 601 was highly enantioselective. Specific rotation ([$\alpha]_D$) and circular dichroism (CD) spectra of the enantiomers are reported here for the first time. Biotransformation kinetics of daidzein and genistein indicated that the C-ring of genistein has a higher susceptibility to bacterial degradation than that of daidzein.

• 한국컴퓨터정보학회논문지
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• 제18권7호
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• pp.149-155
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• 2013

일반적으로 재료절단 문제는 재료를 절단할 수 있는 패턴을 찾고 선형계획법으로 최적의 패턴 수를 찾는다. 그러나 패턴 수는 일반적으로 지수적으로 증가하기 때문에 사전에 모든 패턴을 고려하는 것은 비현실적인 것으로 알려져 있다. 본 논문은 Suliman의 실현 가능 패턴을 구하는 방법을 적용하여 사전에 패턴을 구하는 방법을 적용하였다. 또한, 실현 가능 패턴들을 대상으로 선형계획법이나 근사 알고리즘을 적용하지 않고 정확한 해를 다항시간으로 얻는 방법을 제안하였다. 제안된 방법은 실현 가능 패턴들 중 모든 요구의 1st 발생 빈도가 손실량 0에 모두 분포하는 경우와 다양한 손실량에 분산되어 분포하는 경우로 구분하여 패턴 수를 분배하는 방법을 적용하였다. 제안된 알고리즘을 2개의 데이터에 적용한 결과 모든 데이터에서 정확한 해를 구하는데 성공하였다.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.831-837
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• 2005

Low-temperature adaptation and cryoprotection were studied in Leuconostoc mesenteroides SYl, a strain isolated from Kimchi. L. mesenteroides SY1 cells grown in exponential growth phase at $30^{\circ}C$ were exposed to $15^{\circ}C,\;10^{\circ}C$, and $5^{\circ}C$ for 2, 4, and 6 h, respectively, and then frozen at $- 70^{\circ}C$ for 24 h. Survival ratio was measured after the cells were thawed. The freezing-thawing cycles were repeated four times. Preadapted cells survived better than non-adapted control cells, and the highest survival ratio ($96\%$) was observed for cells preadapted for 2 h at $5^{\circ}C$, whereas control cells showed only $22\%$. The 2D gel showed that two proteins (spots A and B) were induced in cells preadapted at lower temperatures. Spots A and B have the same molecular weight (7 kDa), but the pI was 4.6 for spot A and 4.3 for spot B. The first 29 and 15 amino acid sequences from spots A and B were determined, and they were identical, except for one amino acid. A csp gene was cloned, and nucleotide sequencing confirmed that the gene encoded spot A cold shock protein.