• 미생물학회지
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• 제28권4호
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• pp.351-363
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• 1990

A mutant was selected by NTG treatment of Mycobacterium sp. NRRL B-3805, which was capable of degrading plant sterol to androsta-4-ene-3, 17-dione and yields was higher than NRRL B-3805. Also this mutant produced androst-4-ene-3, 17-dione faster than NRRL B-3805. It described the mode of sitosteroidal degradation, and the interrelation between cell membrane and its attachment to substrate during the sterol degradation process by this mutant and it was compared with Mvcobacterium sp. NRRL B-3805.

• Archives of Pharmacal Research
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• 제14권3호
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• pp.261-265
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• 1991

In a microbial cell-free extract system, side chain cleavage on various sterols and steroids was tested. The cell-free extracts of Mycobacterium sp. NRRL B-3805 showed the side chain cleavage activity on lithocholic acid to form 5$\beta$-androstan-3.17-dione. The properties of the activity were examined.

• 약학회지
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• 제12권3_4호
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• pp.76-84
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• 1968

When 19-hydroxycholesterol acetate was added into CSD-10 in Nutrient Broth or in a mineral salts medium consisting of KH$_{2}$PO$_{4}$(0.1%), $K_{2}$HPO$_{4}$(0.1%), NH$_{4}$NO$_{3}$(0.1%), MgSO$_{4}$(0.02%), CaCl$_{2}$(0.002%), and FeCl$_{3}$(0.005%), a substantial amount of 19-hydroxyandrost-4-ene-3,17-dione was accumulated prior to accumulation of estrone. From this result and all of previous works, a tentative degradation pathway of 19-hydroxycholesterol acetate to estrone by CSD-10 was derived. 19-hydroxyandrost-4-ene-3,17-dione seems to be an attractive intermediate for the synthesis of 19-norsteroids.

• Natural Product Sciences
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• 제17권1호
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• pp.5-9
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• 2011

An alkaloid, 3-methylcanthin-5, 6-dione, was isolated from the stem of Picrasma quassioides (D. Don) Benn. and characterized by comprehensive analyses of its 1D and 2D NMR spectra. It was also evaluated for its cytotoxic activity in vitro against three human cancer cell lines (MDA-MB-231, HT-29 and NCI-N87), using MTT assays. We found that 3-methylcanthin-5, 6-dione exhibited significant anti-inflammatory activity via inhibiting NO production induced in LPS-stimulated murine macrophage RAW264.7 cells. The antioxidant activity of 3-methylcanthin-5, 6-dione was measured by DPPH free radical scavenging assays, hydroxyl radical scavenging assays and reducing power assays. Our results showed that 3-methylcanthin-5, 6-dione has significant biological activities.

• 약학회지
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• 제31권6호
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• pp.402-404
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• 1987

Fermentation processes have been employed to produce 17-ketosteroids from sterol with chelator of Fe$^{2+}$ such as $\alpha$, ${\alpha}'$-dipyriyl. To analyze the products of sterol fermentation, we developed a simple TLC method without interference of the chelator $\alpha$, ${\alpha}'$-dipyridyl, using Ag$^+$ band on TLC plate.

• Bulletin of the Korean Chemical Society
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• 제31권12호
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• pp.3517-3518
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• 2010

• 약학회지
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• 제33권6호
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• pp.365-371
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• 1989

Microbiological conversion of sterols to 17-ketosteroids has been recognized as a source for commercial preparation of steroidal drugs. In order to develop bacterial strains and process with Brevibacterium lipolyticum IAM 1398 capable of converting cholesterol to 1,4-Androstadiene-3,17-dione (ADD) at about 27% yield, we studied on strain improvement, fermentation condition and whole cell immobilization. By using UV and/or NTG as mutagens, a mutant to convert cholesterol to ADD with higher yield than 60% was selected. Better production of ADD was manifested in the case of maltose used as a supplemental carbon source, and yeast extract or soytone as a nitrogen source. Addition of tween 80 (0.05%) as a surfactant beneficial for increasing the productivity. The optimal initial pH of the medium was 6.5 and optimal culture temperature was $30^{\circ}C$. Whole cell immobilization by using carrageenan, agar, alginate and acrylamide was carried out and the activity of conversion was tested. In the case of carrageenan and agar, immobilized cells were active for at least two cycles of fermentation.

• 한국미생물·생명공학회지
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• 제16권3호
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• pp.187-192
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• 1988

Cholesterol로부터 미생물전환 방법에 의한 4-androstene-3,17-dione(AD)의 생성에 대해 연구를 수행했다. 발효액중에 cholesterol의 용해도를 증가시키기 위해 ethanol을 용매로 사용하였는데, ethanol 농도가 2%(v/v)까지는 세균성장이 크게 저해되지 않았다. 미생물전환은 pH를 7.0으로 조절하고, 초기대수 증식기에 cholesterol을 첨가했을 때 효율적으로 AD가 생성되었다. AD 생성을 높이기 위해 cholesterol 첨가방법을 여러 가지로 변환시켰다. 즉, 최종 cholesterol 농도를 0.1% 하여 ethanol에 녹여 간헐적으로 첨가했을 때 가장 높은 수율을 얻었다. Cholesterol을 세번(전체 3g/$\ell$) 첨가했을 때 최종 전환수율이 65%에 도달한 반면, 같은 양의 cholesterol (3g/$\ell$)을 한번에 넣었을 때 40%의 생성수율을 얻었다.

• Bulletin of the Korean Chemical Society
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• 제17권3호
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• pp.299-302
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• 1996

• 한국미생물·생명공학회지
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• 제18권1호
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• pp.12-17
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• 1990

콜레스테롤과 식물스테롤을 AD와 9-Alpha-AD로 분해 하는 Mycobacterium속의 돌연변이주를 분리하였다. 또한, 이 변이주는 스테로이드성 핵의 9Alpha 위치에 Hydroxylation을 시켰다. 이러한 반응은 고리파괴저 해제 없이도 가능하였다.