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Diversity in Lipid Contents and Fatty Acid Composition of Soybean Seeds Cultivated in Korea (국내 유통 콩의 지방함량 및 지방산 조성변이)

  • Kim, Sun-Lim;Lee, Yeong-Ho;Chi, Hee-Youn;Lee, Sun-Joo;Kim, Si-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.52 no.3
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    • pp.348-357
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    • 2007
  • The 117 soybeans seeds were collected from the nine provinces of Korea, and protein and lipid contents, and fatty acid composition levels were evaluated to investigate their relationship. The 100-seed weights of the black soybeans were varied $27.7{\sim}33.1g$, while the 100-seed weight of yellow soybeans were varied $24.6{\sim}36.6g$. Protein and lipid content of the 117 soybean seeds was 38.3% and 17.8%, respectively Protein contents of the 59 black soybean seeds (38.6%) were significantly higher than those of the 58 yellow soybean seeds (37.9%). However, lipid contents of the black soybean seeds (17.6%) were lower than those of the yellow soybean seeds (18.1%). Linoleic and oleic acid composition levels of the 117 soybean seeds were 53.75% and 22.08%. Unsaturated fatty acid levels of soybean seeds showed a statistically significant variability among the nine provinces of Korea, however, the differences were not found in the linoleic (18:2) and oleic acid (18:1) levels. Therefore, it was considered that the significant variability of unsaturated fatty acid were mainly due to the variations of linolenic acid (18:3) level. The composition levels of linoleic, oleic, palmitic (16:0), and linolenic acid in the yellow soybean seeds were 53.43%, 22.73%, 12.23%, and 8.24%, while those of the black soybean seeds were 54.13%, 21.48%, 12.47%, and 8.31%. Obtained results suggested that fatty acid composition levels were varied and possibly influence by the phenotype of seed coat colors. Oleic acid, mono-saturated fatty acid, showed the most remarkable variability between yellow and black soybean seeds, and the composition levels were higher in the yellow soybean seeds. Relationship between unsaturated fatty acid levels and 100-seed weights in the yellow soybean seeds showed a negative correlation (r=-0.513, P<0.01), but no relationship $(r=0.154^{ns})$ was observed in the black soybean seeds.

Quantitative Analysis of Resveratrol in Mulberry Leaves (뽕잎의 품종별.시기별 resveratrol 함량 변이)

  • Kim, Hyun-Bok;Kim, Jung-Bong;Kim, Sun-Lim;Koh, Seong-Hyouk;Seok, Young-Seek;Kim, Yong-Soon;Sung, Gyoo-Byung;Kang, Pil-Don
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.1
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    • pp.23-28
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    • 2011
  • Resveratrol has been associated with reduced cardiovascular disease and reduced cancer risk. This phytochemical has been reported in a number of plant species including grapes, peanuts and pine trees in response to stress such as fungal infection, heavy metal ions or UV irradiation. The objective of this study was to determine the resveratrol contents in leaves of mulberry varieties at different collecting times. Quantitative analysis of 16 cultivars showed a range of $102{\sim}466{\mu}g/100g$ on dry weight basis (which is equivalent to $25{\sim}116.5{\mu}g/100g$ on fresh weight basis). Resveratrol contents in mulberry leaves was higher in autumn than spring, and higher in fully matured leaves than in juvenile leaves. Among the tested samples, 'Kaeryangppong', 'Sugeppong' and 'Cheongilppong' collected in the middle of October showed high resveratrol contents of $838{\mu}g/100g$, $803{\mu}g/100g$, $800{\mu}g/100g$ on dry weight basis, respectively. Especially mulberry leaves dried in the shade showed of $1,030{\mu}g/100g$ on dry weight basis in resveratrol content, this result may contribute to utilization of mulberry leaves.

Effects of Hairy Vetch and Rye Cover on Weed Occurrences and Minor Cereal Growth (피복작물처리에 따른 잡곡의 생육과 잡초발생)

  • Jeon, Seung-Ho;Lee, Se-Hun;Oh, Se-Yun;Kim, Young-Ju;Kim, Kyung-Moon;Kim, Seok-Hyun;Hwang, Jae-Bok;Yoon, Seong-Tak;Shim, Sang- In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.134-139
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    • 2011
  • This study was conducted to evaluate the weed suppressing effects of two winter cover crops, hairy vetch and rye in foxtail millet and sorghum fields in 2010. Crop growth and development and weed occurrences in the fields were examined to know the efficiency of proposed cropping system. In hairy vetch treated plots, heading of minor cereals occurred early. The heading date was earlier by 1 day and 2 days in sorghum and foxtail millet, respectively. However, rye treatment delayed heading by 12 days and 8 days in sorghum and foxtail millet, respectively. Besides he effect of cover crop on ear emergence of crops, the Besides changed growth-related characteristics. Plant height, chlorophyll content and chlorophyll fluorescence of sorghum ere increased in hairy vetch treatment by 46.4% 88.7% and 7.9%, respectively. In foxtail millet, the characteristics ere also increased by 45.6%, 50.9% and 37.8%, respectively, s compared to control. Yields of sorghum and foxtail millet were increased by 105.1% and 135% as compared o control by hairy vetch treatment, respectively. However, he yields of cereal crops were decreased by rye cover crop treatment, the yields of sorghum and foxtail millet were decreased by 25.8% and 119.1%, respectively. Rye cover crop treatment inhibited crop growth suggesting nitrogen starvation in rye treated plots. In rye treatment, plant height, chlorophyll contents and chlorophyll fluorescence of sorghum ere slightly decreased by 7.1%, 10.8% and 10.8%, respectively, as compared to control whereas the inhibitory effects were greater in foxtail millet. Weed occurrences based n weed number in hairy vetch and rye plots were reduced n weed number in hairy vetch and rye plots were reduced y 27% and 20%, respectively. The smothering effect was weakened or disappeared after heading of crops. Weed number and dry weight in hairy vetch plot were increased by 159% and 55.2%, respectively, as compared to control. The results implied that weed suppressing of cover crops could be reduced drastically after heading of crops.

Expression of OsPTs-OX Transgenic Rice in Phosphate-Deficient Condition (무인산 조건에서 OsPTs 유전자 도입 형질전환벼의 인산흡수 반응)

  • Song, Song-Yi;Yi, Gi-Hwan;Park, Dong-Soo;Seo, Jong-Ho;Son, Beom-Young;Kim, Do-Hoon;Nam, Min-Hee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.3
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    • pp.264-272
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    • 2011
  • It needs to develop high phosphate-available rice that is able to minimize environmental pollution caused by phosphate fertilizer. Then we have transformed 4 rice transporter genes, OsPT(Oryza sativa Phosphate Transporter)1, OsPT4, OsPT7 or OsPT8, to rice (Oryza sativa cv. Dongjin) via Agrobacterium-mediated transformation. We tested adaptation in the P-deficient condition of Dongjin (parental) and each transgenic line in the pot and the field conditions. Definite physiological changes have been observed in OsPTs transgenic lines including culm length, root formation and heading date. Phosphate uptake at harvesting stage was about three times higher in OsPT1-OX (overexpression) and OsPT4-OX than in Dongjin (wt) without P application. There are no variations in total phosphate-content of brown rice of OsPT1-OX in spite of high phosphate uptake. Practically the expression of OsPT1 has contributed to stabilize grain production without P fertilization in rice cultivation than Dongjin.

Introduction of Stay Green Mutant for the Development of Black Seed Coat and Green Cotyledon Soybean Variety (녹색자엽 검정콩 품종 육성을 위한 Stay green 변이체 활용)

  • Kang, Sung-Taeg;Seo, Min-Jung;Moon, Jung-Kyeong;Yun, Hong-Tae;Lee, Young-Ho;Kim, Si-Ju;Hwang, Young-Sun;Lee, Suk-Ki;Choung, Myoung-Gun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.3
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    • pp.187-194
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    • 2010
  • The soybean stay green mutant genotype (SSG) derived from the nuclear gene, d1d2, and cytoplasmic gene, cytG, inhibit the breakdown of chloroplast in the leaves, pod walls, seed coats, and embryos during maturity. Soybean seed with black seed coat and green cotyledon (SBG) are preferred than black seed coat with yellow cotyledon (SBY) especially for cooking with rice and as source of traditional food in Korea. The researchers evaluated the seed's chlorophyll content of SSG and introduced SSG to the SBG variety breeding program. The seed chlorophyll content of SSG with d1d2 was $39.93{\sim}60.80\;{\mu}g/g$ and SSG with cytG $38.08{\sim}39.89\;{\mu}g/g$. The Korean SBG variety which was derived from SSG with cytG, contains $16.35{\sim}37.73\;{\mu}g/g$. The composition of seed chlorophyll differs according to the genetic background of SSG genotype. Inheritance study showed that cotyledon color was segregated 15:1 (yellow:green) at $F_2$ seed indicating two recessive genes control green cotyledon as revealed by previous study. Only less than 3% soybean lines showed black seed coat with green cotyledon among crosses SBY and SSG (d1d2). Results showed that SSG with d1d2 can be used as a good source for SBG with high chlorophyll content in the seed cotyledon, but due to the complex genetic behavior, breeding resource of SBG with d1d2 should be prepared to improve the breeding efficiency for development SBG variety.

Agronomic Characteristics of Sorghum bicolor (L.) Moench Germplasm (수수 유전자원의 작물학적 특성)

  • Yoon, Seong-Tak;Xu, Zhen Yu;Zhang, Qing-Yu;Kim, In-Sook;Kim, Tae-Ho;Nam, Jung-Chang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.1
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    • pp.83-90
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    • 2010
  • Crop characteristics of 179 sorghum (Sorghum bicolor L. Moench) resources collected in Korea were investigated in order to establish basic data for the improvement of crop breeding. Spike types of 179 sorghum resources were classified as 5 types of open-loose type, broom-tillering type, half broom-tillering type, extreme-compact type and compact type, of which broom-tillering type was the highest ratio of 38.0% (68 plant resources) of 179 germplasm. In the existence and nonexistence of spike awn in 179 sorghum resources, 28.5% (51 plant resources) showed existence of spike awn, whereas the rest of 71.5% (128 plant resources) had no spike awn. Seed type was classified as 5 types, of which circle-shape showed the highest ratio of 43.0% (77 plant resources) and the lowest was inclined-circle shape by 7.3% (13 plant resources). Seed color was classified as 4 colors of brown, white, whitish brown, and yellowish brown, of which yellowish brown was the highest ratio of 84.4% (151 plant resources) among them. Days from seeding to heading date showed the range from 67 to 88 days with the average of 77.4 days and the highest frequency proportion of it was the group from 76 to 80 days, which occupied 37.4% (67 plant resources) of 179 germplasm. 179 sorghum resources showed high variation in the range of culm length with the average of 253.8 cm and group from 92 cm to 360 cm of culm length showed the highest frequency proportion of 20.6% (37 plant resources). Spike length showed the range from 15 to 49 cm with the average of 28.8 cm and the highest frequency distribution of it was the group from 31 to 35 cm with the proportion of 26.3% (47 plant resources), whereas the lowest was below 15 cm with the proportion of 0.6%. Days from seeding to physiological maturity showed the range from 110 to 146 days with the average of 125.5 days and the highest frequency proportion (39.7%) of it was the group from 111 to 115 days, while there were also occupied with 2 plant resources below 110 days and 23 plant resources over 141 days among 179 germplasm. Number of grains per spike showed the range from 163 to 4,532 grains with the average of 2,068.6 grains and group from 1,601 to 2,000 grains per spike was the highest frequency distribution with the proportion of 25.7% (46 plant resources). 1000 grains weight showed the range from 10.6 to 38.1g with the average of 25.6g and group from 26.0 to 30.0g was the highest frequency distribution with the proportion of 44.1% (79 plant resources).

Effect of Planting Ratio of Male Sterility (MS) and Restorer Line (RL) on Fatty Acid Content and Composition during Seed Filling Period in $F_1$ Seed of Brassica napus L. (유채의 $F_1$ 종자 생산시 종자친과 화분친의 재식비 및 등숙시기별 종자의 지방산 분석)

  • Ku, Yang-Gyu;Yang, Sun-Young;Jung, Yong-Su;Kim, Hyun-Sung;Suh, Mi-Chung;Ahn, Sung-Ju
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.3
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    • pp.226-231
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    • 2010
  • The objective of this experiment was determined to investigate the effect of the planting ratio of Male Sterility (MS) to Restorer Line (RL) and harvesting time on fatty acid compositions under $F_1$ seed production of Brassica napus L. For rapeseed seed production, two experiments were conducted in the open fields. One experiment studied planting ratios of MS to RL (4:2, 10:2, or 10:1) were planted and investigated fatty acid composition at 40, 45, 50, 55, and 60 days after flowering, the other $F_2$ seeds were analyzed on fatty acid compositions of harvested seeds at five sequential stages. The results showed that fatty acid compositions of developing seeds were influenced by MS:RL planting ratios and $F_2$ hybrid treatments and contaminated level of fatty acid compositions, erucic acid, were unaffected by planting ratio of MS to RL. Fatty acid compositions such as palmitic acid (C16:0), stearic acid (C18:0) and linoleic acid (C18:2) contents decreased during seed maturation period in $1^{st}$ and $2^{nd}$ experiments. In contrast, oleic acid (C18:1) content relatively increased up to 55days after flowering. At day 60 after flowering, oleic acid content was unaffected by MS:RL planting ratios and $F_2$ seeds treatments. Aspects of related gene expression of fatty acid synthesis such as SAD, FAD1 and FAD2 were followed exactly to changes of fatty acid composition during seed maturation. These results suggest that MS ratio may be enlarged and RL may be reduced, indicating this ratio will be useful for rape seed production.

Occurrence of Insect Pest from Organic Seed Producing Field of Minor Grain Germplasms (잡곡유전자원을 이용한 유기종자생산 포장의 해충발생 소장)

  • Kim, Jeong-Soon;Ko, Ho-Cheol;Yoon, Sung-Tag;Cho, Yang-Hee;Kim, Jung-Gun;Shim, Chang-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.1
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    • pp.58-64
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    • 2010
  • This study was carried out to screen the occurrence of insect pest on the organic seed producing field of minor grain germplasms, Pearl millet (Setaria italic L.), Sorghum (Sorghum bicolor L.), and Common millet (Panicum miliaceum L.) in Gangwon-do from 2008 to 2009. The artificial pheromone traps successfully attracted an Oriental corn borer, Ostrinia furnacalis (Guenee) on Pearl millet, but the Army worm, Pseudaletia separata, was not attracted in all of the minor grain fields. The ratio of damaged plants of Oriental corn borer and Army worm were appeared in order with Pearl millet, Common millet, and Sorghum. The morphological diversities of plant bug were shown as four kinds of species, Eastern green stinkbug (Nezara antennata), Brown-marmorated stinkbug (Halyomorpha halys), Sloe bug (Dolycoris baccarum), and Bean bug (Riptortus clavatus), on the organic seed producing field of minor grain. The average occurrence density of Eastern green stinkbug was the highest level in the three kind of minor grains, Pearl millet, Common millet, and Sorghum in 2008 and 2009. The dominant species are the Eastern green stinkbug and the Bean bug in Pearl millet and Common millet. The Sloe bug and the Bean bug possessed the highest population density in Sorghum.

Composition in Milling Recovery Ratio of Rice Cultivars, Ilpumbyeo and Chucheongbyeo (일품벼와 추청벼의 도정률 차이의 작물학적 요인분석)

  • Kim, Deog-Su;Kim, Sun-Lim;Song, Jin;Hur, On-Suk;Kim, Jung-Tae;Lee, Choon-Ki;Kim, Jae-Hyun;Kim, Kee-Jong;Suh, Sae-Jung
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.3
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    • pp.308-313
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    • 2008
  • This study was carried out to analysis the factor effected by milling rice rate, and to provide the developing rice varieties and cultivation technology. Panicle numbers per spike of Ilpumbyeo and Chucheongbyeo were 105 and 70, respectively. The primary branch panicle rate was Ilpumbyeo 56.2% and Chucheongbyeo 61.4%. The secondary panicle rate of Ilpumbyeo and Chucheongbyeo was 43.3% and 37.9%, respectively. Grain filling rate using specific gravity showed that Ilpumbyeo was the lower filling rate than Chucheongbyeo. Hull weight per one grain was Ilpumbyeo 41.9 mg and Chucheongbyeo 3.92 mg, and hull weight per rough rice 1 kg was Ilpumbyeo 157.36 g and Chucheongbyeo 151.31 g. In milling rate, brown rice ratio by ripening degree of llpumbyeo and Chucheongbyeo with combine harvest was 81.22% and 82.52%. Milled/brown rice ration of llpumbyeo and Chucheongbyeo showed 92.14% and 92.51%. The milling recovery ratio of llpumbyeo was decreased 1.67% then Chucheongbyeo, and the difference was mainly due to the hull weight (0.61%), milled/brown rice ratio (0.37%) and ripening degree (0.69%). Although the varietal differences were found in hull weight and millied/brown rice ratio between llpumbyeo and Chucheongbyeo, the ripening degree was considered as the factor that could be reduced by cultivation technology and post-harvest management.

Effects of Medium and Planting Density on Growth and Yield of Seed Potatoes Grown in a Wick Hydroponic System (배지 및 재식밀도가 심지양액재배 씨감자의 생육 및 수량에 미치는 영향)

  • Kim, Chan-Woo;Song, Chang-Khil;Park, Jung-Sik;Mun, Hyun-Ki;Kang, Young-Kil;Kang, Bong-Kyoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.3
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    • pp.251-255
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    • 2008
  • This study was carried out in 2002 to determine the usefulness of Jeju scoria for a component of a growth medium and optimum planting density of 'Dejima' seed potatoes (Solanum tuberosum L.) grown in a wick hydroponic system. The minitubers ($7.0{\pm}0.2\;g$) produced in an aeroponics system were planted at five planting densities (3 to 15 tubers/box; 19 to $95\;tubers/m^2$) in polystyrene boxes (51 cm long $\times$ 31 cm wide $\times$ 20 cm high) containing two media (perlite + peatmoss and Jeju scoria + peatmoss 1:2, v/v mixtures). There were no significant interactions between medium and planting density for the growth and tuber yield traits. Shoot growth and the number of tubers per plant were not significantly affected by the media. However, tuber yield was higher in the perlite + peatmoss mixture than in the Jeju scoria + peatmoss mixture. The percentage of underdeveloped plants ranged from 8.3 to 14.7% at four lower planting densities (3 to 12 tubers/ box), and was 25.8% at the highest planting density (15 tubers/box). As planting density was increased from 3 to 15 tubers per box, seed potato (${\geq}5\;g$ tuber) number increased from 101 to 269 and yield from 6.3 to $11.6\;kg/m^2$. These results indicate that the perlite + peatmoss mixture might be more suitable for seed potato production in the wick hydroponic system, and considering the percentage of underdeveloped plants and tuber yield, optimum planting density would be 56 to $76\;tubers/m^2$ in the system depending on availability of seed potatoes.