• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.4
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• pp.234-244
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• 2013

This study was conducted to determine the factors affecting to seed characteristics and seedling growth of Zelkova serrata. The seeds were collected from sixteen populations of Z. serrata and the seed characteristics (i.e., seed length, width, weight and full seed rate) were measured. We also measured the 1-year-old seedling growth of each population at nursery. All seed characteristics showed significant differences in population level. Seed length and seed width were positively correlated with latitude, longitude, altitude and distance from coast line of sampling site. Seed length and seed width also negatively correlated with annual mean temperature, mean temperature of growing season (Mar.~Oct.). The ratio of seed length/seed width showed inverse trend in case of seed length and seed width. Seed weight/1,000 grains had no correlation with geographic factors but showed negative correlation with annual mean dryness index. Seed weight/L showed negative correlation with latitude and longitude and positive correlation with mean temperature of growing season of sampling site. Full seed rate showed negative correlation with latitude, longitude and annual mean dryness index of sampling site. There were significant differences among populations, among family within population and among individuals within family in seedling growth. Height and diameter of root collar of seedling showed negative correlation with longitude and mean humidity of growing season of sampling site. Height growth of seedlings was not correlated with any seed characteristics but, diameter at root collar showed low negative correlation with seed weight/1,000 grains and seed weight/L. We discussed the implications of the results in view of tree improvement of Z. serrata.

• Korean Journal of Environmental Agriculture
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• v.35 no.4
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• pp.278-285
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• 2016

BACKGROUND: Uptake patterns of ${\alpha}$-, ${\beta}$-isomers and sulfate metabolite of endosulfan (ED) by radishes grown in treated soils with ED concentrations of 2 and 10 mg/kg were investigated to establish soil management guidelines for ensuring the safety of radishes from ED residues. METHODS AND RESULTS: All samples of soils and radish plants separated into shoot and root parts were analyzed for ED residues using a gas-chromatography mass spectrophotometer, and the results were used to calculate the bioconcentration factor (BCF), indicating the ratio of ED concentrations between radishes and soils. During the experimental period, uptake and distribution rates of ED-sulfate in radishes were the highest, followed by ${\alpha}$- and ${\beta}$-ED. The BCF values to initial ED concentrations in soils were greater for root parts (0.0077 to 0.2345) than for shoot parts (0.0002 to 0.0429) and used to obtain regression equations by time. Long-term BCFs estimated by the obtained equations ($R^2$ of 0.86 to 1.00) were evaluated with the maximum residue limit (0.1 mg/kg) of ED for radishes, in order to suggest safe management guidelines of ED for radish-cultivating soils. CONCLUSION: Suggested guidelines showed the significant dependency on duration for radish cultivation and exposed concentration of ED in soil.

• Korean Journal of Environmental Agriculture
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• v.35 no.4
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• pp.270-277
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• 2016

BACKGROUND: Significant amount of bisphenol A has been released from the manufacturing process of plastics, epoxy resins, dental material and flame retardants. Bisphenol A has been detected at trace levels in wastewater, surface water, landfill leachate and drinking water. However, the residual survey of bisphenol A has not been performed in agricultural reservoir beside agricultural environment cultivating crops. This study was conducted to monitor the residual bisphenol A in national agricultural reservoirs and understand a level of contamination of bisphenol A in the agricultural environment in Korea. METHODS AND RESULTS: The water and water sediment were collected at agricultural reservoirs in Chungnam, Chungbuk, Kyunggi, Jeonnam, Jeonbuk, Kyungnam and Kyungbuk province. Bisphenol A was analyzed by the LC-MS/MS with triple quad 4500. The recovery of water and water sediment in the agricultural reservoirs showed the level of 95.7~97.2% and 91.5~100.3%, respectively. CONCLUSION: Bisphenol A was detected at the level of $0.05{\mu}g/L{\sim}0.18{\mu}g/L$ and $0.1{\mu}g/kg{\sim}34{\mu}g/kg$ in water and water sediment of the reservoirs, respectively. Based on the results, the residue of bisphenol A will be tried in the crops surrounding these agricultural reservoirs where bisphenol A detected above $10{\mu}g/kg$ of bisphenol A.

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.2
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• pp.93-104
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• 1996

A field experiment was conducted in Chungju and Jungwon to evaluate growth characteristics, dry matter yield, protein yield and palatability of intercropping comparing with monocropping forage crops by the use of sorghum $\times$ sudangrass hybrid(S.S.H.), five forage soybeans and two forage cowpeas of superior to selected experiment of varieties. All eight treatment plots were replicated three times and cutting date were cut July 6 and September 9. 1. The mean leaf number of S.S.H. in intercropping T2 treatment showed high compared to T1 treatment in all treatment except for Hwangkeum treatment, but T3 treatment was lower than the other treatments. In the leaf number of soybean, Jangbaek treatment was the highest as 47 leaves per plant, cowpea treatments showed high above 50 leaves. The stem diameter of S.S.H. in Jangyeob treatment of T2 treatment was 9.6mm. In legume, cowpea of T3 treatment was high as ranged from 7.9mm to 8.2mm. In stem hardiness of S.S.H., TI treatment was the lower than the other treatments, while Jangbaek treatment of T2 treatment was the highest as $2.0kg/cm^2$. In legume crops, Jangbaek and Baekun treatment were the highest as $1.6kg/cm^2$, but T3 treatment was very low as $0.3~0.6kg/cm^2$. 2. In the mean leaf ratio of S.S.H., T1 treatment was higher than T2 and T3 treatment as 34.9%. In the legume crops, Togyu treatment was the highest as 40.9%. In the mean tiller number of S.S.H., T2 treatment showed high compared to TI treatment, but T3 treatment of was rather decrease than The tiller number of S.S.H. in Jangyeob treatment of T2 treatment was the highest as 4.6 per plant. The dead stubble of S.S.H. was high in order to T3>T1>T2 treatment>, T3 treatment was highly about 2 times compared to T2 treatment. 3. S.S.H. as T3 treatment was resistant to lodging, but T3 and T1 treatment showed highly lodging. In the legums, T3 treatment was resistant to lodging, but T2 treatment showed highly lodging. In the S.S.H. of T2 treatment, the leaf of summer depression was not occurrence, but T3 treatment was higher than the other treatments. 4. The palatability of domestic animals was high in order of T2>Tl>T3, especially Togyu treatment of T2 treatment was the highest at the holstein and deer, and Jangyeob treatment of T2 treatment was the highest in the Korean native cattle. But T3 treatment was lower than other treatments in the holstein and Korean native cattle. 5. Fresh yield of monocropping(T1) was the highest as 94, 650kg/ha, while dry matter yield in Jangyeob treatment of T2 treatment was the highest as 15, 575/ha But fresh yield and dry matter yield of T3 treatment were the lowest(P<0.05). 6. Protein yield in T2 treatment was high, especially Jangyeob treatment of T2 treatment was the highest as 1, 605.5 kg/ha T3 treatment was low in spite of high protein content, because dry matter yield was lower than the other treatments. In conclusion, among T1, T2 and T3 treatment, Jangyeob, Togyu and Baekun treatments of T2 treatment showed optimum varieties for intercropping with S.S.H., because they showed high Dry matter yield, protein yield and palatability.

• Journal of Embryo Transfer
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• v.16 no.2
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• pp.79-89
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• 2001

Steroid hormones control the expression of many cellular regulators, and a role thor estrogen in mouse oocytes has been well documented. The preovulatory $E_2$increment is generally accepted as the endocrine process regulating induction of in vivo oocyte maturation To address whether the activity of the T-type $Ca^{2+}$ channel is altered by 17 beta-estradiol ( $E_2$), we examined the actions of $E_2$on the calcium channel of mouse oocytes and early embryos. Oocrtes were collected from the oviduct of mice treated with pregnant mare's serum gonadotropin (PMSG) and human choronic gonadotropin (hCG). Whole cell voltage clamp technique and confocal microscopy were used to examine that $E_2$increase intracellular $Ca^{2+}$ concentration ([C $a^{2+}$]$_{i}$ ) via voltage dependent $Ca^{2+}$ channel (VDC) and estrogen receptor (FSR), and $E_2$concentration by the use of radioimmunoassay (RIA) were examined in mouse. The results obtained were as follows: The peak of $Ca^{2+}$ current induced by $E_2$increased 122% to 1.50$\pm$0.03 nA from 1.23$\pm$0.21 nA (n=15) in the presence of 5 mM extracellular $Ca^{2+}$ concentration ([C $a^{2+}$]$_{o}$ ). The increased $Ca^{2+}$ current was temporally associated with $Ca^{2+}$ transients. The intracellular $Ca^{2+}$ level increased 207%~30 s following the addition of 1${\mu}{\textrm}{m}$ $E_2$(relative fluorescence intensity: 836.4$\pm$131.2 for control, n=10, 1736.4$\pm$192.0 in the presence of $E_2$, n=10). $E_2$increased amplitude of $Ca^{2+}$ current and [C $a^{2+}$]$_{i}$ . $E_2$-induced $Ca^{2+}$ current and $E_2$concentration in blood were showed difference on the stage of embryo. These results suggest that $E_2$modulate $Ca^{2+}$ channel to increase $Ca^{2+}$ influx.$Ca^{2+}$ influx.

• Journal of Embryo Transfer
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• v.20 no.3
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• pp.271-277
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• 2005

This study was undertaken to determine the normal appearance of the postpartum uterine involution. Postpartum changes in uterine shape, architecture, echogenicity and diameter were monitored with ultrasonography in 8 Cocker spaniel bitches. The excretory period of vaginal discharges in 8 normal bitches of uterine involution was finished completely at 23.20$\pm$2.77 days (Mean$\pm$SD) postpartum. The short axis shape of the uterus was varied from polygonal to circular. This lasted until 1600$\pm$2.12 days Postpartum, during which time the short axis uterine shape gradually changed to circular. Also, the long axis shape of the uterus was created a beaded appearance of the horns until 25.60$\pm$2.51 days postpartum. The uterine diameter was decreased not only in the placental sites from 24.20$\pm$2.06mm at 1 day to 13.18$\pm$0.84mm at 7 days postpartum, but also in the interplacental sites 14.26$\pm$2.22mm at 1 day, 9.81$\pm$0.7mm at 7 days postpartum. There was a general trend of decreasing uterine diameter, which occurred more rapidly at the placental sites. In conclusion, normal postpartum uterine involution in Cocker spainel bitches appeared to be completed around 68 days postpartum by gross findings such as vaginal discharges, and by ultrasongraphic findings, uterine shape and echogenicity.

• Journal of fish pathology
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• v.18 no.3
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• pp.269-276
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• 2005

The concentrations of quinolones (oxolinic acid; OXA, norlloxacin: NRF & ciprofloxacin: CPF) after oral administration of single doses (20 mg/kg B.W.) were investigated in carp (Cyprinus carpio) kept in freshwater at 20-23$^{\circ}C$. The distribution of the drug was studied after treatment. At points timed, from 1 h to 96 hrs after administration, blood (B), liver (L), kidney (K) and muscle (M) from 5 individuals in each group were collected for analyse with microbiological bioassay method. The peak concentrations were measured at 8 h (L), 12 h (B and K) and 24 h (M) after administration regardless of treated drugs. Considerably high concentrations of CPF (13.8-19.6${\mu}g/m{\ell}$) NRF (11.8-16.9${\mu}g/m{\ell}$) and OXA (10.8-13.9 ${\mu}g/m{\ell}$) were revealed during the 24 h. At the last time point of the experiment (96 h), concentrations of all three quinolones were: OXA, 2.3-6.3 ${\mu}g/m{\ell}$ ; NRF, 3.1-4.5 ${\mu}g/m{\ell}$ ; CPF, 3.0-5.5${\mu}g/m{\ell}$ in samples. The concentrations decreased subsequently, indicating a first rapid redistribution, followed by a slow phase of elimination. The steady state was observed in blood (12-36 h), liver (12-96 h) and muscle (36-96 h) after the initiation of treatment with OXA. Concerning the compartmental concentrations, (L, K. and M/B concentration ratio), the fluctuation of the ratio was founded at different time points, among drugs. For CPF, highest tissue ratios were prolonged in the order of L>K>M (0.65-1.2/0.82-0.93/1.0-1.7) during the experiments. On the other hand, NRF presented L>K>M (0.65-1.3/0.86-1.0) till 24 h, but L>M>K (0.89-1.26) at 36-96 h. OXA showed L>K>M (0.95-2.1) at 1-8 h, M>K>L (0.51-1.0) at 12-36 hand M>L>K (1.0-2.3) at 48-96 h, respectively.

• Journal of fish pathology
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• v.2 no.1
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• pp.19-30
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• 1989

The purpose of this study was to obtain the fundamental data for health assessment of cultured fishes. A series of hematological studies and blood chemistries were made on cultured israeli carps from May, 1988 to May, 1989. The results of this study were as followings : 1. The blood constituents of healthy carps were Hct $32{\pm}3.4%$, Hb $8.3{\pm}0.9g/l$, RBCs $132{\pm}27.6({\times}10^4cm^3)$), Total protein $2.7{\pm}0.7g/dl$, GOT $143{\pm}19.5U$, GPT $50{\pm}14.2U$, Total glucose $70{\pm}12.6mg/dl$ and BUN $7.5{\pm}1.9mg/dl$, etc. 2. The blood constituents by change of water temperature with the control of $23^{\circ}C$ showed the decrease in Hct and RBCs at $18^{\circ}C$, and the increase in Hct, RBCs and glucose at $28^{\circ}C$. 3. The blood constituents by change of dissolved oxigen with the control of 4.5ppm showed the increase in Hct and RBCs at 3ppm, and the decrease in Hct and RBCs at 7ppm. 4. In the case of intramuscular injection of Streptococcus sp. with $10^6cells$/fish infection dose, there showed marked decrease in Hct, RBCs, glucose and BUN with inflammatory reaction, and the fishes were recovered in 16 days. 5. In the case on intraperitoneal injection of Streptococcus sp. with $10^4cells$/fish infection dose, there showed decrease in Hct, RBCs and BUN with inflammatory reaction, but there were no dead cases. 6. In the case of intramuscular injection of Edwardsilla tarda with $1.2{\times}10^8cells$/fish infection dose, there showed decrease in Hct and RBCs, and increase in TCHO, GOT, GPT and BUN with marked inflammatory reaction, and 60% of inoculated fishes were died within 3 or 4 days.

• The Journal of the Petrological Society of Korea
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• v.12 no.1
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• pp.16-31
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• 2003

The granites in the southern Gimcheon area can be divided into two parts, marginal hornblende biotite granodiorite (Mgd) and central biotite granodiorite to granite (Cgd). Mgd and Cgd are gray in color and display gradational contact relations and are mainly composed of coarse-grained and medium-grained rocks, respectively. Mgd has more frequent and larger mafic enclaves than Cgd, and the two granites partly show parallel foliation at thire contact with gneisses. From representative samples of the granites, K-Ar biotite ages of 197∼207 Ma were obtained. Considering the blocking temperature of biotite, it is suggested that the emplacement age of the granitic magma was probably late Triassic. The anorthite contents of plagioclases in Mgd display less variation than those of Cgd, indicating that Mgd crystallized within a narrow range of temperatures. In the Al$\_$total/-Mg diagram, the biotites from the granites plot within the subalkaline field, and the smooth slope indicates differentiation from a single magma. All amphiboles from the granites belong to magnesio-hornblende. The linear trends of major oxides, AFM and Ba-Sr-Rb indicate that Mgd and Cgd were fractionally differentiated from a single granitic magma body crystallizing from the margin inwards. The relations of modal (Qz+Af) vs. Op, K$_2$O vs. Na$_2$O, Fe$_2$ $O_3$ vs. FeO, Fe$\^$+3/(Fe$\^$+3/+Fe$\^$+2/) and K/Rb vs. Rb/Sr show that they belong to I-type and magnetite-series granitic rocks developed by the progressive melting products of fixed sources. REE data, normalized to chondrite value, have trends of enriched LREE and depleted HREE together with weakly negative Eu anomalies.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.10 no.1
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• pp.36-43
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• 2007

Purpose: The ultrasonography (USG) and computed tomography (CT) are popular diagnostic tools for the diagnosis of acute appendicitis in children, but there are many debates about their clinical significance. The purpose of this study is to clarify the clinical significance of USG, CT and follow-up CT performed subsequently to USG, especially in perforated acute appendicitis in children. Methods: We have reviewed 419 cases of surgically confirmed acute appendicitis in children under the age of sixteen, who had been treated in Inje University Ilsan Paik Hospital from March 2002 to February 2006. All the clinical data including the results of USG and CT were collected and analyzed. Results: Sensitivity, specificity, positive and negative predictive values of USG were 98.7%, 96.8%, 98.1%, 97.8% in non-perforation group and 90.8%, 100%, 100%, 81.9% in perforation group. Those of CT were 96.4%, 100%, 100%, 96.5% and 86.6%, 100%, 100%, 87.5% respectively. Those of follow-up CT after USG were 100%, 100%, 100%, 100% and 87.5%, 100%, 100%, 92.0% respectively. The duration of using antibiotics in seven patients showed positive correlation with the interval between two imaging studies (r=0.0472, p=0.019). There was no statistical significance of correlation when these imaging studies performed within 30 hours together. Conclusion: In most of the cases, single choice between USG and CT would be enough to diagnose the acute appendicitis in children. But, it may be helpful to perform CT as early as possible subsequently to USG when there is discrepancy between initial USG and clinical impression.