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DNA Barcoding of Scolelepis (Parascolelepis) papillosa (Annelida, Spionidae) in Korea, with Additional Taxonomic Notes

  • Lee, Geon Hyeok;Lee, Ha-Eun;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.37 no.4
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    • pp.349-353
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    • 2021
  • Scolelepis (Parascolelepis) papillosa (Okuda, 1937), originally described from a single incomplete individual from Jeju Island in Korea, was collected from the intertidal sandflats of Soan Island (Jeollanam-do province) in Korea. The examined specimens of S. (P.) papillosa agree well with the original description in having the papillae on the basal sheath of the palps, presence of occipital antenna, absence of notochaetae in chaetiger 1, branchiae completely fused with notopodial postchaetal lamellae at the anterior chaetigers, and neuropodial hooded hooks appearing from chaetiger 16. In this study, the sequences of partial mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA (16S rDNA), and the nuclear 18S ribosomal DNA (18S rDNA) of the species were determined. We also provide the detailed description and illustrations on this species based on the complete specimens newly collected in this study.

Prevalence and molecular characteristics of 16s rRNA methylase gene rmtB in amikacin resistant Escherichia coli isolated from South Korea

  • Belaynehe, Kuastros Mekonnen;Won, Ho Geun;Yoon, In Joong;Yoo, Han Sang
    • Korean Journal of Veterinary Research
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    • v.59 no.3
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    • pp.157-160
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    • 2019
  • The production of rmtB-encoded 16S rRNA methylases has emerged as a novel mechanism promoting high-level resistance toward aminoglycosides in Gram-negative bacteria. Between 2015 and 2017, 636 distinct commensal Escherichia (E.) coli isolates were collected from different farms in South Korea to determine the prevalence and molecular characteristics of rmtB. The positive rates of rmtB between all the isolates and amikacin-resistant isolates were 1.1 and 100%, respectively. High-level aminoglycoside resistance could be transferred by conjugation from rmtB-positive donors to higher amikacin-resistance efficacies. This is the first report of 16S rRNA methylase-encoding genes in E. coli isolated from food-producing animals in Korea.

Diagnostic distal 16p11.2 deletion in a preterm infant with facial dysmorphism

  • Hyun, Ju Kyung;Jung, Yu Jin
    • Journal of Genetic Medicine
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    • v.15 no.2
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    • pp.115-119
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    • 2018
  • The 16p11.2 microdeletion has been reported in patients with developmental delays and intellectual disability. The distal 220- kb deletion in 16p11.2 is associated with developmental delay, autism spectrum disorder, epilepsy, and obesity at a young age. We have reported a case of distal 16p11.2 deletion syndrome in a preterm infant with unusual facial morphology and congenital heart disease. We suggest using chromosome microarray analysis to detect chromosomal abnormalities in newborns, especially preterm infants with unusual morphologies.

PWR 16×16 사용후핵연료 집합체 다운엔더 개념설계

  • Kim, Yeong-Hwan;Lee, Jae-Won;Lee, Han-Su;Park, Geun-Il;Lee, Jeong-Won;Jo, Gwang-Hun
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2012.05a
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    • pp.141-142
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    • 2012
  • 다운엔더 개념설계요건 설정을 위해서 PWR $16{\times}16$ SF집합체 제원을 분석하였다. 또한 수직에서 수평으로 전환될 때 충격량을 최소화하기 위해서 모의집합체 충격실험을 수행하였다. 그 결과 수직에서 수평으로 회전되는 각속도(30초/$90^{\circ}$)를 고려할 때 평균값은 약 0.1 g, 최대 약 0.3 g 정도가 되어 거의 충격을 받지 않음을 알 수 있다. 주요 설계요건으로 그리이드(grid)에 가해지는 수평클램프(clamp) 힘은 240kg, 하부노즐에 가해지는 수직클램프 힘은 900kg 이내로 하였다. 다운엔더의 개념설계를 위해서 기구학적 특성을 반영하였고, 전환시간을 30초/$90^{\circ}$로 하였다. 원격 유지 보수성을 향상하기 위하여 Solid Works 프로그램 툴(tool)을 이용하여 5개의 주요 모듈을 구성하였고, SF 집합체 다운엔더 개념을 3D로 설계하였다.

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Synthesis of Pd and Pt Based Low Cost Bimetallic Anode Electrocatalyst for Glycerol Electrooxidation in Membraneless Air Breathing Microfluidic Fuel Cell

  • Panjiara, Deoashish;Pramanik, Hiralal
    • Journal of Electrochemical Science and Technology
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    • v.12 no.1
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    • pp.38-57
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    • 2021
  • The different weight ratios of Pd to Pt, i.e., 16:4, 10:10, 4:16 in Pd-Pt/C and Pd (20 wt. %) /C electrocatalysts with low metal loading were synthesized for glycerol electrooxidation in an air breathing microfluidic fuel cell (MFC). The cell performance on Pd-Pt (16:4)/C anode electrocatalyst was found best among all the electrocatalysts tested. The single cell when tested at a temperature of 35℃ using Pd-Pt (16:4)/C, showed maximum open circuit voltage (OCV) of 0.70 V and maximum power density of 2.77 mW/㎠ at a current density of 7.71 mA/㎠. The power density increased 1.45 times when cell temperature was raised from 35℃ to 75℃. The maximum OCV of 0.78 V and the maximum power density of 4.03 mW/㎠ at a current density of 10.47 mA/㎠ were observed at the temperature of 75℃. The results of CV substantiate the single cell performance for various operating parameters.

Elastic α-12C Scattering with the Ground State of 16O at Low Energies in Effective Field Theory

  • Ando, Shung-Ichi
    • Journal of the Korean Physical Society
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    • v.73 no.10
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    • pp.1452-1457
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    • 2018
  • Inclusion of the ground state of $^{16}O$ is investigated for a study of elastic ${\alpha}-^{12}C$ scattering for the l = 0 channel at low energies in effective field theory. We employ a Markov chain Monte Carlo method for the parameter fitting and find that the uncertainties of the fitted parameters are significantly improved compared to those of our previous study. We then calculate the asymptotic normalization constants of the $0^+$ states of $^{16}O$ and compare them with the experimental data and the previous theoretical estimates. We discuss implications of the results of the present work.

Relation of Chitosan oligosaccharide-induced Melanin Production to The Activity and Expression of Tyrosinase in B16 Melanoma Cells

  • Yun, Yoon-Mi;Young, Cho-Nam;Kim, Kyung-Won;Lee, Ji-Yun;Kim, Chang-Jong;Soo, Sim-Sang
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.123.1-123.1
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    • 2003
  • To investigate the effect of chitosn oligosaccharide on skin care, we measured tyrosinase activity and melanin production in B16 melanoma cells, and elastase and hyaluronidase activity. Chitosan oligosaccharide itself did not have any anti-oxidant activity in DPPH radical scavenging, and did not affect the proliferation of B16 melanoma cells. Chitosan oligosaccharide dose-depednetly increased mealnin production in the absence or presence of MSH. However, chitosan oligosaccharide did not have any influence on the tyrosinase activity and tyrosinase expression in B16 melanoma cells. (omitted)

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First Record of Scolelepis (Scolelepis) daphoinos (Annelida: Polychaeta: Spionidae) in South Korea

  • Lee, Geon Hyeok;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.37 no.3
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    • pp.229-234
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    • 2021
  • Scolelepis (Scolelepis) daphoinos is newly reported in Korean fauna. This species can be distinguished from its congeners by the following characteristics: the presence of reddish pigment patches on the posterior part of the prostomium, notopodial postchaetal lamellae that are partially fused to the branchiae, and the presence of only the bidentate hooded hooks. The morphological diagnosis and photographs of S. (S.) daphoinos are provided. The partial mitochondrial cytochrome c oxidase subunit I (COI), 16S ribosomal DNA(16S rDNA), and the nuclear 18S ribosomal DNA (18S rDNA) sequences from Korean specimens of S. (S.) daphoinos were determined. Species identification was supported by a comparison of DNA barcode sequences of COI and 16S rDNA with morphological examination from the specimens of type locality, China.

Assessment of the gastrointestinal microbiota using 16S ribosomal RNA gene amplicon sequencing in ruminant nutrition

  • Minseok Kim
    • Animal Bioscience
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    • v.36 no.2_spc
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    • pp.364-373
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    • 2023
  • The gastrointestinal (GI) tract of ruminants contains diverse microbes that ferment various feeds ingested by animals to produce various fermentation products, such as volatile fatty acids. Fermentation products can affect animal performance, health, and well-being. Within the GI microbes, the ruminal microbes are highly diverse, greatly contribute to fermentation, and are the most important in ruminant nutrition. Although traditional cultivation methods provided knowledge of the metabolism of GI microbes, most of the GI microbes could not be cultured on standard culture media. By contrast, amplicon sequencing of 16S rRNA genes can be used to detect unculturable microbes. Using this approach, ruminant nutritionists and microbiologists have conducted a plethora of nutritional studies, many including dietary interventions, to improve fermentation efficiency and nutrient utilization, which has greatly expanded knowledge of the GI microbiota. This review addresses the GI content sampling method, 16S rRNA gene amplicon sequencing, and bioinformatics analysis and then discusses recent studies on the various factors, such as diet, breed, gender, animal performance, and heat stress, that influence the GI microbiota and thereby ruminant nutrition.

An Explainable Deep Learning Algorithm based on Video Classification (비디오 분류에 기반 해석가능한 딥러닝 알고리즘)

  • Jin Zewei;Inwhee Joe
    • Annual Conference of KIPS
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    • 2023.11a
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    • pp.449-452
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    • 2023
  • The rapid development of the Internet has led to a significant increase in multimedia content in social networks. How to better analyze and improve video classification models has become an important task. Deep learning models have typical "black box" characteristics. The model requires explainable analysis. This article uses two classification models: ConvLSTM and VGG16+LSTM models. And combined with the explainable method of LRP, generate visualized explainable results. Finally, based on the experimental results, the accuracy of the classification model is: ConvLSTM: 75.94%, VGG16+LSTM: 92.50%. We conducted explainable analysis on the VGG16+LSTM model combined with the LRP method. We found VGG16+LSTM classification model tends to use the frames biased towards the latter half of the video and the last frame as the basis for classification.