• Journal of Microbiology and Biotechnology
• /
• v.11 no.2
• /
• pp.234-241
• /
• 2001

The C-terminal 393 bp region of the human interleukin-16 (IL-16) gene was cloned and expressed in E. coli along with mammalian cell lines. Recombinant IL-16 expressed from E. coli was 22 kDa on SDS-PAGE and showed 260% of chemoattractant activity at a concentration of $0.1\;{\mu}g/ml$. HeLa, COS, and Neuro-2a cells were transduced by recombinant retrovirus vector pLNC/IL-16/IRES/TK and the intracellular and secreted amounts of IL-16 produced by HeLa/IL-16/TK, COS/IL-16/TK, and Neuro-2a/IL-16/TK cells were determined by enzyme-linked immunosorbent assay (ELISA). HeLa/IL-16/TK $(1{\times}10^5)$ and COS/IL-16/TK $(1{\times}10^5)$ cells secreted 36.1 and 13.3 ng of IL-16 for 48 h, respectively. Forty-nine ng and 86.4 ng of IL-16 remained in the cell lysates of HeLa/IL-16/TK and COS/IL-16/TK. Intracellular and secreted amounts of IL-16 from Neuro-2a/IL-16/TK $(5{\times}10^5)$ cells during 24 h cultivation were 50 ng and 3.3 ng, respectively. Also, HeLa and COS cells wee stably transfected with mammalian expression vector pCRIII/IL-16. Both culture media and cell lysates prepared from HeLa/IL-16 cells and COS/IL-16 cells showed chemoattractant activity ranging from 190% to 460% as compared to the control experiment. Expression of the herpes simplex virus thymidine kinase (HSV0tk) gene in pLNC/IL-16/ IRES/TK bicistronic retroviral expression vector was verified by performing a genciclovir (GCV) sensitivity assay. Finally, IL-16 repressed Tat-transactivated human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR) promoter activity.

• Genomics & Informatics
• /
• v.16 no.4
• /
• pp.16.1-16.3
• /
• 2018

• Biomedical Science Letters
• /
• v.11 no.4
• /
• pp.421-427
• /
• 2005

To evaluate the diagnostic significance of p16 overexpression in human hepatocellular carcinoma (HCC), we analyzed p16 status and growth inhibitory patterns by p16 overexpression in HCC cell lines having different pRE status. SKHep1 and SNU449 cells show homozygous deletion of p16. The p16 gene in SNU398 cell is inactivated at posttranscription level. Adenovira1-p16 (Ad-p16) infection inhibits the cell growth in Hep3B, SNU398, and SNU449. Failure of growth inhibition in SKHepl results from the low transduction efficiency of adenovirus. The p16-mediated growth inhibition shows G 1 phase arrest in pRE-positive SNU449 but not in pRE-negative Hep3B. These results suggest that therapeutic efficacy of p16 gene might be considered on the transduction efficiency and the toxicity of adenoviral vector. Beside, growth inhibitory effect of p16 could be exerted through either pRE-dependent or -independent pathway.

• Proceedings of the Korean Society of Broadcast Engineers Conference
• /
• 2009.11a
• /
• pp.63-66
• /
• 2009

기존의 H.264/AVC 비디오 표준은 고화질 비디오 부호화를 지원하지만 고해상도에 특화된 요소 기술이 도입되지 않아 만족할만한 성능을 보이지 못한다. 현존하는 동영상 압축 표준 중 가장 뛰어난 H.264/AVC 표준의 인트라 $16{\times}16$ 예측은 매크로블록에 인접한 최대 33개의 주변 화소를 이용하여 매크로블록에 속한 256개의 화소 값을 예측한다. 특히, 전체 예측 모드 중 수직과 수평 예측 모드에서는 16개의 수직 또는 수평 위치에 위치한 주변 화소로 전체 매크로블록 내의 화소 값을 예측하므로 매크로 블록의 끝으로 갈수록 예측의 정확도가 떨어져 부호화 비트가 증가한다. 고화질 영상에서는 인트라 $16{\times}16$ 모드로 부호화되는 블록이 많으므로 수행되므로 인트라 $16{\times}16$ 예측의 정확도를 높일 수 있는 기술이 필요하다. 본 논문에서는 기존의 H.264/AVC의 예측 방법보다 예측 정확도가 높은 새로운 라인 기반 $16{\times}16$ 인트라 예측 방법을 제안한다. 일반적으로 편평한 특성을 보이는 인트라 $16{\times}16$ 블록이라도 좀 더 가까운 화소를 참조 화소로 사용하면 예측의 정확도를 높여 부호화 비트를 줄일 수 있다. 이를 이용하여 제안하는 알고리즘에서는 인트라 $16{\times}16$ 블록에서 16개 화소 한 줄을 단위로 예측 및 부호화를 수행한다. 1080p HD급 테스트 영상을 이용하여 실험한 결과, 기존의 H.264/AVC FRExt High 프로파일에 비해 평균 약 6.92%의 부호화 비트를 감소시킬 수 있음을 보였다.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.37C no.11
• /
• pp.1045-1053
• /
• 2012

Link-16 is a widely used TDL (Tactical Data Link) which uses TDMA (Time Division Multiple Access). Link-16 is a very low rate system, so it supports small size of data like tactical message and voice. However, there are related works to transmit situation awareness information like image due to the increasing interest about EBO(Effect-Based Operation), recently. Special TDMA scheduling is needed not static TDMA of Link-16 for image transmission because image data has much larger size than the existing tactical data. In this paper, we proposed Link-16K which enhances the Link-16 MAC. The proposed Link-16K is compatible with Link-16, and includes dynamic TDMA, new packing method, and an efficient retransmission scheme for image transmission effectively. We can see that image transmission delay is reduced and channel utilization is increased through simulation results of proposed idea.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.44 no.6
• /
• pp.54-62
• /
• 2007

In this paper, we significantly reduces the amount of computation for intra 16$\times$16 mode decision in H.264 by applying the fast algorithm, which obtains the transformed prediction residual with fewer computations. By extending the existing intra 4$\times$4 mode decision, we propose the new algorithm for fast intra 16$\times$16 mode decision. The proposed algorithm uses partial transform coefficients which consist of one DC and three adjacent AC coefficients after 4$\times$4 transform in the intra 16$\times$16 mode decision. Theoretical analysis and experimental results show that the proposed algorithm can reduce computations up to 50% in the intra 16$\times$16 mode decision process with unnoticeable degradation.

• Tuberculosis and Respiratory Diseases
• /
• v.44 no.4
• /
• pp.796-805
• /
• 1997

Background : It is clear that deregulation of cell cycle progression is a hallmark of neoplastic transformation and genes involved in the $G_1$/S transition of the cell cycle are especially frequent targets for mutations in human cancers, including lung cancer. p16 gene product, one of the G1 cell-cycle related proteins, that is recently identified plays an important role in the negative regulation of the the kinase activity of the cyclin dependent kinase (cdk) enzymes. Therefore p16 gene is known to be an important tumor suppressor gene and is also called MTS1 (multiple tumor suppressor 1). No more oncogenes have been reported to be frequently related to multiple different malignancies than the alterations of p16 gene. Especially when it comes to non-small cell lung cancer, there was no expression of p16 in more than 70% of cell lines examined. And also it is speculated that p16 gene could exert a key role in the development of non-small cell lung cancer. This study was designed to evaluate whether p16 gene could be used as a candidate for gene therapy of non-small cell lung cancer. Methods : After the extraction of total RNA from normal fibroblast cell line and subsequent reverse transcriptase reaction and polymerase chain reaction, the amplified p16 cDNA was subcloned into eukaryotic expression plasmid vector, pRC-CMV. The constructed pRC-CMV-p16 was transfected into the NCI-H441 NSCLC cell line using lipofectin. The changes of G1 cell-cycle related proteins were investigated with Western blot analysis and immunoprecipitation after extraction of proteins from cell lysates and tumor suppressive effect was observed by clonogenic assay. Results : (1) p16(-) NCI-H441 cell line transfected with pRC-CMV-p16 showed the formation of p16 : cdk 4 complex and decreased phosphorylated Rb protein, while control cell line did not. (2) Clonogenic assay demonstrated that the number of colony formation was markedly decreased in p16(-) NCI-H441 cell line transfected with pRC-CMV-p16 than the control cell line. Conclusion : It is confirmed that the expression of p16 protein in p16 absent NSCLC cell line with the gene transfection leads to p16 : cdk4 complex formation, subsequent decrease of phosphorylated pRb protein and ultimately tumor suppressive effects. And also it provides the foundation for the application of p16 gene as a important candidate for the gene therapy of NSCLC.

• Microbiology and Biotechnology Letters
• /
• v.32 no.1
• /
• pp.72-77
• /
• 2004

Previously, we synthesized a novel Cyclin-dependent kinase inhibitor, MCS-5A. Also, we investigated the involvement of cell cycle regulatory events during MCS-5A-mediated apoptosis in HL-60(+p16/-p53) cells with up-regulation of p16 protein expression. In contrast, apoptosis was not observed in A549(-p16/+p53) cells. Therefore we propose that $p16^{INK4A}$ is a key enzyme for inducing apoptosis. In the present studies, we have explored the mechanism of $p16^{INK4A}$ -mediated cytotoxicity and the role of p16.sup INK4A/ overexpression in the induction of apoptosis in human tumor cells. The tumor suppressor gene $p16^{INK4A}$ is known as a cyclin-dependent kinase inhibitor (CKI) and cell cycle regulator. We expressed wild type $p16^{INK4A}$ in pcDNA3.1 vector and then transfected into non-small cell lung cancer (NSCLC) cell expressing different statue of p16$^{INK4A}$, p53 gene〔A549(-p16/+p53), H1299(-p16/-p53) and HeLa(+pl6/+p53) cell line〕. TUNEL assay (including propidium iodide staining following transfection of these cell line with pcDNA3.1-pl6) indicate that p16$^{INK4A}$-mediated cytotoxicity was associated with apoptosis. This is supported by studies demonstrating an induction of caspase 3 cleavage due to the transfection of A549, H1299 and HeLa cells with pcDNA3.1-pl6. These results suggest that p16$^{INK4A}$ has a new function of inducing apoptosis which is not related with the function of tumor suppressor gene p53.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.26 no.6
• /
• pp.1109-1115
• /
• 1997

Hydrocarbons were analyzed in irradiated beef, pork, dried and seasoned beef, dried anchovy, dried squid, dried shrimp, and fish paste to determine whether the hydrocarbons can be used as markers for detecting post-irradiation of the imported meat and fish products. The samples were irradiated at 0.5, 1, 3, and 6 kGy. Fat was extracted with hexane, and hydrocarbons were separated from the fat through Florisil column. The hydrocarbons were analyzed with GC. Hydrocarbons 15:0, 16:1, 17:1, 16:2, 17:2, and 16:3 in beef and pork, 17:1, 16:2, and 17:2 in dried and seasoned beef, 16:2 in dried anchovy, 16:1 and 17:1 in dried squid, 16:1, 17:1, and 16:2 in dried shrimp, and 16:1, 16:2, and 16:3 in fish paste were detected in the irradiated samples, but not in the unirradiated, so that the hydrocarbons may be used as makers for detecting post-irradiation of each item.

• Journal of the Korea Society for Simulation
• /
• v.28 no.4
• /
• pp.33-43
• /
• 2019

Link-16 is a data link that provides joint interoperability to the US Navy, Air Force and NATO. Currently, the military relies entirely on foreign SW and tools for test environment, tactical simulation training and interoperability verification test for Link-16 operation. Therefore, it is necessary to develop Link-16 based operation environment test tool. In this paper, Link-16 network operational performance analysis simulator was developed by analyzing the function of Link-16 foreign tools. It also implements the SIMPLE standard interface for interworking with foreign SW and tools. The functional model for Link-16 network operation performance analysis consists of pre-analysis, real-time operational analysis, and post-analysis functional model. Each functional model test was performed through SIMPLE interworking with foreign SW and tools. Link-16 network operation performance analysis If we replace foreign SW through simulator, we can perform tactical training, network design verification and operation (scenario) verification for our military.