• The Korean Journal of Nuclear Medicine Technology
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• v.14 no.1
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• pp.8-12
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• 2010

Purpose: In $^{18}F$-FDG automated synthesizer, deliver is done in automated mode after synthesis until the dispenser. After the delivery, the yield is calculated from the radioactivity which was read by the dose calibrator located in the dispenser. However, when the distance between the automated synthesizer and the dispenser is far, there are $^{18}F$-FDG residues, which results in loss of the amount of $^{18}F$-FDG. This study investigated the usefulness of a method that minimizes $^{18}F$-FDG residues. Materials and Methods: The structure of the tubing between the (TRACERlab Mx FDG; GE.) and the dispenser is that the distance is 8 m and the internal diameter is 1/16 inch. The synthesis process of The module goes through the synthesis process of trap, synthesis, delivery in the automated module. The time taken for synthesis is about 25 to 26 minutes, after which rinsing is done. However, after rinsing, as the distance of the tubing increased, there were 10~13% of $^{18}F$-FDG residues. Therefore, a method of using push syringe and $N_2$ gas in manual mode to minimize $^{18}F$-FDG residues is analyzed. Results: In manual mode, there were $^{18}F$-FDG residues of 4~5% for the push syringe, and there were $^{18}F$-FDG residues of less than 1% for the $N_2$ gas, which showed that the method using $N_2$ gas had superior usefulness. Also, there were no $^{18}F$-FDG residues in the cleaning the next day. Conclusion: The distance between the synthesizer and the dispenser needs to be reduced as much as possible, to reduce the rate of loss of $^{18}F$-FDG resulting from the distance of the tubing. However, in case the distance between the synthesizer and the dispenser has to be increased due to the system structure, using push syringe and $N_2$ gas simultaneously is a useful method for minimizing $^{18}F$-FDG residues.

• Korean Journal of Soil Science and Fertilizer
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• v.25 no.1
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• pp.26-37
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• 1992

Fifty-five field experiments were conducted in order to find out some useful indices for the prediction of N-supplying capability(NSC) of soils under cultivation of corn in Pennsylvania over 3 years from 1986. Contents of $NO_3-N$, absorbance at 200 nm of the extract from soil with 0.01M $NaHCO_3$ were identified to be used as indices before planting. Methods for the estimation of organic nitrogen available later in the growing season(KCLA-N, PBBA-N, UV260 nm absorbance of $NaHCO_3$ extract) were not to be used as good indices individually, but when those are combined together with inorganic $NO_3-N$ showed a highly significant correlationship with the NSC. The year of an even distribution of rainfall, 1987, gave the highest significant correlationship between NSC and the indices. For soils of the same texture with slightly different physical properties, combined indices obtained from physico-chemical factors improved the degree of predictability when the grades of soil slope, depth of Ap were considered at the same time. More futher researches such as this need to be done before any conclusive result can be drawn.

• Journal of Environmental Science International
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• v.9 no.3
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• pp.223-228
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• 2000

The purpose of this study is to evaluate the purification capacity of pollutants by column test with the tidal flat sediment. Sediment materials were taken from Chunjangdae tidal flat which located in Chungnam Seochungun. The column tests were conducted on four conditions(R1 : raw sewage filtered by G2 filter, R2 : sterilized sewage after filtered by GF/C filter, R3 : R2 /filtered(membrane) seawater (1:1), R4 : R2 /filtered(membrane) seawater(1:2)). The results of this study may be summarized as followed ; The removed COD by column tests were increased according to increasing the quantity of sewage. During the column tests of 580min, the total removed ammonia nitrogen were 90.1mg for R1, 81.0mg for R2, 27.6mg for R3 and 4.1mg for R4. The result was similar to COD experiment. During the 580min, the total removed total-phosphates were 3.4mg for R1, 4.2mg for R2, 5.6mg for R3 and 2.0mg for R4. The removal efficiency of Pb and Cd for R3 and R4 reactor were higher than R1 and R2 reactor. The remove of heavy metal by the column test was high in sample with seawater. But in the initial 20min, the adsorbed Pb and Cd showed about 3% of the total adsorbed Pb and Cd during 580min.

• Journal of Aquaculture
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• v.15 no.2
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• pp.95-101
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• 2002

During the periods from lily to October, 2000 in Hongseong and lucy to October, 2001 in Taean in the west coast of Korea, the following environmental conditions prevailed : water temperature : 22.0~26.817, salinity 27.23 ~30.80%, dissolved oxygen 4.12 ~6.26 ml/l, pH 7.89 ~8.09, phosphate 0.39 ~0.65 $\mu m$ , inorganic nitrogen 5.05~9.26 $\mu m$, suspended solid 5.4~20.8 mg/l and chemical oxygen demand 1.12~1.87 mg/l. The B-shaped veliger larvae of the Ark shell occurred in maximum number at $25^{\circ}C$ prevailing from mid-August at Hongseong and Taean. Full grown larvae reached maximum abundance from late August. To identify the effectiveness of the substratum for spat collection, raschel net were tested to Larval settlement. The most effective depth to collect the larvae in natural environment was the collectors suspended at 7~8 m depth. At these depths, about 49 to 94 spats were found on the collector (40$\times$50 cm), The growth of shell height (Y) to shell length (X), and total weight (W) to shell length (L) could be formulated as follows respectively: Hongseong: SH = 0.7168 SL -0.6466 ( $r^2$ = 0.9839), TW = $0.0001SL^{3.1705}$ ($r^2$ = 0.9882) Taean: SH = 0.736 SL -0.8824 ($r^2$ : 0.9899), TW : 0.00005 $SL^{3.3731}$ ($r^2$ : 0.9899)

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.10
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• pp.1443-1451
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• 2014

Increasing cost and scarcity of maize has stimulated the use of alternative feed sources (AFS) in the diets of cattle. In this study, we investigated the effects of partial or total replacement of maize on nutrient digestibility, growth performance, blood metabolites, and economics in Limousin crossbred feedlot cattle. Forty-five $Limousin{\times}Luxi$ crossbred bulls were randomly assigned to the three treatment groups, orthodox diet (OD; 45.0% maize), partial replacement diet (PRD; 15% maize, 67% AFS), total replacement diet (TRD; 0% maize, 100% AFS). The growth feeding trial lasted for 98 days. Dry matter intake (DMI) and average daily gain (ADG) were recorded. The digestion trial was carried out after the end of the growth trial. Total faeces and feed samples were measured daily. Digestibilities of dry matter (DM) and organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) were calculated. After the feeding trial, blood metabolites were measured in 12 animals from each group. Initial and final body weights did not differ significantly among treatment groups (p>0.05). The ADG and DMI were 1.72 and 8.66, 1.60 and 9.10, and 1.40 and 9.11 kg/d for OD, PRD, and TRD, respectively. The PRD and TRD exhibited lower ADG (p<0.01) and higher DMI (p<0.01) than OD. The DMI (%body weight) was comparable between groups (p>0.5). Feed efficiency of PRD and TRD were lower than OD (p<0.01). The DM digestibility decreased with reduced level of maize (p = 0.10), OM digestibility was higher in OD (p<0.05), and CP, NDF and ADF digestibilities were similar for all groups (p>0.05). Blood urea nitrogen (mg/dL) in PRD and TRD was higher than OD (p<0.01), while other blood parameters did not differ significantly. Feed costs ($/head/d) were 1.49, 0.98, and 0.72 for OD, PRD, and TRD, respectively (p<0.01). Feed costs per kg gain ($) were significantly lower for PRD (0.63) and TRD (0.54) than OD (0.89; p<0.01). Overall profit ($/head) and daily profit ($/head/d) did not differ significantly between treatments (p>0.05), although TRD showed the highest economic benefits overall (p<0.01). While a traditional diet maximized the growth rate, partial or total replacement of dietary maize with AFS proved economically feasible due to their lower costs and comparable nutrient digestibilities of DM, CP, NDF, and ADF. Partial replacement may prove economically competitive in the current situation of China.

• Journal of Nutrition and Health
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• v.1 no.2
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• pp.61-85
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• 1968

The experiment was carried out on eight healthy male subjects of 19-22 years old and they were maintained The experiment was carried out on eight healthy male subjects of 19-22 years old and they were maintained under the rice diet, the general mixed diet and the high animal protein diet for 7 days of each experimental period, respectively. The composition of each diet was indicated in Table 3. The allowance' of each nutrient per head per day in each diet were as follows. a) In the rice diet; protein:139.5 g (animal source:64.6 g, vegetable source:74.9 g), fat:25.3 g (animal source:11.3 g, vegetable source:14.0 g), carbohydrate: 644.0 g, calcium:982.3 mg, phosphorus: 2369.2mg, thiamine:2.1 mg, riboflavin: 1.6 mg, and 3211 Cal. b) In the general mixed diet; protein: 97.4g (animal source: 10.5 g, vegetable source: 86.9 g), fat:40.3 g (animal source:7.1 g, vegetable source:33.2 g), carbohydrate:620.7 g, calcium:887.1m g, phosphorus:2200.8m g, thiamine: 1.8m g, riboflavin:1.9m g, and 3158 Cal. c) In the high animal protein diet; protein: 135.6 g (animal source:68.8 g, vegetable source:66.8 g), fat:32.5g (animal source:17.9 g, vegetable source:14.6 g), carbohydrate:930.9 g, calcium: 626.0 mg, phosphorus: 1998.9 mg, thiamine: 1.5 mg, riboflavin: 1.5 mg, and 3194 Cal. The absorption rates of protein, fat, carbohydrate, calcium, phospherus, thiamine and riboflavin in each diet were observed. The results obtained were summarized as follows: 1. The absorption rates of protein and fat in the rice diet were 88.t % and 71.3.%. 2. The absorption rates of protein and fat in the general mixed diet were 83.4% and 86.4%. 3. The absorption rates of protein and fat in the high animal protein diet were 86.8% and 82.4%. 4. The nitrogen balances in the rice, the general mixed and the high animal protein diet groups were +5.7g, +2.3g and +4.0g respectively. 5. The absorption rates of carbohydrate in each diet were all above 95 % and so seemed to be almost completely absorbed except the cellulose in the diets. 6. The calory utilization rates in each diet were all above 93 %. 7. The minimum absorption rates of protein, fat and calory were 83.8%, 63.3%, and 89.7% in the rice diet, 80.0%, 80.9% and 85.9%, in the general mixed diet and 83.6%, 75.7% and. 89.3 % in the high animal protein diet respectively. Therefore, it is assumed that these data might be significant in practical use. 8. The protein absorption rates of the rice diet (88.1 %) was better than that of the general mixed diet (83.4 %). 9. The fat absorption rates of the general mixed diet and the high animal protein diet (86.4% and 82.4%) were signficantly better than that of the rice diet (71.3%). 10. The calcium absorption rates of each diet, rice diet, the general mixed diet and the high animal protein diet were 41.6%, 36.0%, and 27.7%, respectively. 11. The phosphorus absorption rates of each diet were 51.4%, 56.0% and 52.3%, respectively.12. The phosphorus absorption rate seemed better than that of calcium. 13. The thiamine absorption rates of each diet seemed 27.0 %, 42.9 % and 29.5 %, respectively. 14. The riboflavin absorption rates of each diet seemed above 30.6%, 27.1%, and 39.3% respectively. 15. The excretion amounts of thiamine or riboflavin were much more than the amounts ingested of the both vitamins. Therefore, the certain amount of both vitamins seemed to synthesize in the intestine.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.334-341
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• 2006

This study was to investigate the growth of planted red pine (Pinus densiflora S. et. Z.) seedling, soil properties and understory vegetation structure after fertilizer treatments [unfertilized plot (control), CF plot (Combination Fertilizer), UF plot (Urea Formaldehyde Fertilizer)] in a Pinus densiflora stand planted after the forest fires in Gosung, Gangwon province. The height growth rates of seedlings in four years were 264% in unfertilized, 404% in CF, and 388% in UF plots, respectively. The root collar diameters were increased 340% in unfertilized, 454% in CF, and 427% in UF plots, respectively. No significant changes occurred in soil total nitrogen and potassium ion ($K^+$) with the fertilization. However, available $P_2O_5$, content in the soil surface (0-15 cm) increased with the fertilizer application. Soil organic matter increased significantly with fertilizer treatments, while gradual decrease occurred in unfertilized plots. Sodium ion ($Na^-$) decreased in all sites. Soil pH, CEC, calcium ion ($Ca^{2+}$) and magnesium ion ($Mg^{2+}$) contents were not significantly different among treatments. Although Shannon's species diversity index and species richness in understory vegetation did not change with fertilizer treatments, vegetation cover rates in forest floor increased significantly with the fertilization. These results suggest that the increase of pine seedling growth and vegetation cover rates with fertilization could enhance soil stabilization in forest tire areas.

• Journal of Chest Surgery
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• v.33 no.9
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• pp.697-706
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• 2000

Background: Isolated lung perfusion(ILP) was developed as a new treatment approach to non-resectable primary or metastatic lung cancer, because of its ability to reduce systemic toxicity while delivering high-dose chemotherapeutic agents to the target organs. This research was planned to evaluate the direct toxic effect of high-dose cisplatin to the lung tissue during isolated lung perfusion. Material and Method: Fifteen mongrel dogs were divided in the perfusate for 40 minutes. The second group was composed of 5 mongrel dogs which underwent ILP with cisplatin 2.5 mg/Kg added to the perfusate for 30 minutes and 10 minutes with washing solution without cisplatin. The third group underwent the same procedure as the second group except cisplatin 5.0 mg/Kg in the perfusate. Activities of serum angiotensin converting enzyme(ACE), tumor necrosis factor-$\alpha$(TNF-$\alpha$), and concentration of serum lactate dehydrogenase(LDH) and blood urea nitrogen/creatinine (BUN/Cr) were analyzed in each groups at the time of pre-perfusion, 1 hour, 1 day, 1 week, and 2 weeks after ILP. Result: Serum ACE activities before and 1 hour, 1 day, 1 week, and 2 weeks after ILP in control group were 45.1$\pm$6.3, 44.6$\pm$9.3, 46.7$\pm$9.5, 50.8$\pm$9.1, 46.1$\pm$4.3 U/L. Those in cisplatin 2.5 and 5.0 mg/Kg groups were 49.4$\pm$12.6, 39.0$\pm$8.6, 42.3$\pm$15.9, 50.0$\pm$2.6, 53.8$\pm$8.3 and 55.5$\pm$12.3, 47.0$\pm$6.3, 45.1$\pm$6.9, 74.8$\pm$19.5, 60.2$\pm$12.0 U/L, respectively. Serum TNF-$\alpha$ activities in each group before and after ILP were 5.0$\pm$1.5 / 7.7$\pm$2.2 / 6.6$\pm$2.5 / 4.3$\pm$1.3 / 5.2$\pm$1.1(control), 8.7$\pm$1.6 / 9.9$\pm$2.2 / 7.9$\pm$1.5 / 6.3$\pm$2.2 / 7.4$\pm$2.4 (cisplatin 2.5 mg/Kg), and 6.9$\pm$0.7 / 8.9$\pm$3.4 / 7.9$\pm$4.0 / 3.3$\pm$0.9 / 5.8$\pm$1.3 pg/ml(cisplatin 5.0 mg/Kg). Mean LDH levels of each group were 225.7 / 271.3 / 328.9 / 350.8 / 255.7(control), 235.7 / 265.7 / 336.0 / 379.5 / 299.2 (cisplatin 2.5 mg/Kg), and 259.6 / 285.2 / 340.6 / 433.4 / 292.4 IU/L(cisplatin 5.0 mg/Kg). So there was no significant difference in serum ACE, TNF-$\alpha$, and LDH activity changes after ILP between the 3 groups. And, there was no significant changes in BUN/Cr in each groups, which was independent of ILP and perfused concentration of cisplatin. In addition, all dogs survived the ILP and there was no significant evidence of pulmonary vascular injury after 2 weeks of ILP with cisplatin. Conclusion: There was no harmful effect of cisplatin to the lund tissue of the mongrel dog up to 5.0 mg/Kg in perfusate. Therefore, it is perceived to be safe and effective to deliver high-dose cisplatin to the lung without pulmonary toxicity and renal damage with ILP.

• Journal of Embryo Transfer
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• v.13 no.3
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• pp.313-321
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• 1998

This study was conducted to investigate the effects of equilibration and dilution methods on the survival rate of vitrified IVM-IVF bovine blastocysts. Vitrification solution was composed with 20% glycerol, 20% ethylene glycol, 3/8 M sucrose and 3/8 M dextrose in D-PBS supplemented with 20% FBS (GESD). Embryos were equilibrated in 1 of 3 methods: 3-step (El), 2-step (E2), or 1-step (E3), and after loading into 0.25-ml straws, were plunged into liquid nitrogen. After warming in water bath at 2$0^{\circ}C$, cryoprotectants were diluted in 1 of 3 methods: 1) D1(VS+1/2 M sucrose, 1/2 M sucrose and l/4 M sucrose), 2) D2 (1/2 M sucrose and 1/4 M sucrose), or 3) D3(1/2 M sucrose only). All procedures except warming were conducted at room temperature. Survival and hatching rates of blastocysts and expanded blastocysts following equilibration methods were 50 and 83.6%, and 27.8 and 67.3%, respectively in El, which were significantly higher (P〈0.01) than those of E2 (16.7 and 23.2%, and 7.4 and 12.5%, respectively) and 23 (0 and 3.7%, and 0 and 0%, respectively). Survival and hatching rates of expanded blastocysts were significantly (P〈0.01) higher than those of blastocysts in El. Survival rates of blastocysts and expanded blastocysts following dilution methods were 52% and 80.6% in D2, which were significantly higher (P〈0.05) than those of D1 (29.6 and 48.3%) and D3 (47.2 and 63.8%). Hatching rates of blastocysts were similar in D1, D2 and D3, however in expanded blastocysts, that of D2(61.3%) was significantly higher (P〈0.01) than that of D1(34.5%). Survival rates of expanded blastocysts in D1 and D2, and hatching rates in D2 and D3 were significantly higher(P〈0.01) than those of blastocysts. These results indicate that the viability of vitrified blastocysts was improved by the several steps of equilibration, and by 2-steps dilution after warming, independently of their stage of development. The results also indicated that the expanded blastocysts are more profitable to vitrification than blastocysts.

• Journal of Food Hygiene and Safety
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• v.31 no.1
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• pp.42-50
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• 2016

The effects of freezing and thawing conditions on microbiological quality and microstructure change of inoculated (Listeria monocytogenes and Campylobacter jejuni) and non-inoculated chicken breasts were investigated. Chicken breasts were frozen with air blast freezing (-20, -70, and $-150^{\circ}C$), ethanol ($-70^{\circ}C$) and liquid nitrogen ($-196^{\circ}C$) immersion freezing. There were no significant differences on the populations of L. monocytogenes inoculated with chicken breasts under different freezing conditions. However, air blast freezing ($-20^{\circ}C$) resulted in significant reductions for total aerobic bacteria and C. jejuni compared to the control and other freezing treatments. The frozen samples were thawed with (hot or cold) air blast, water immersion, and high pressure thawing at $4^{\circ}C$ and $25^{\circ}C$. the populations of total aerobic bacteria, and yeast and mold in the frozen chicken breast increased by 5.78 and 4.05 log CFU/g after water immersion thawing ($25^{\circ}C$) treatment. After five freeze-thaw cycles, the populations of total aerobic bacteria, yeast and mold, and C. jejuni were reduced by 0.29~1.40 log cycles, while there were no significant differences (P > 0.05) in the populations of L. monocytogenes depending on the freeze-thaw cycles. In addition, the histological examination of chicken breasts showed an increase in spacing between the muscle fiber and torn muscle fiber bundles as the number of freeze-thaw cycles increased. These results indicate that freezing and thawing processes could affect in the levels of microbial contamination and the histological change of chicken breasts.