• Toxicological Research
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• v.14 no.2
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• pp.163-169
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• 1998

Fluoride (F), over a narrow concentration range, increases bone formation. Aluminum (Ai) too is biphasic in its action on bone, being mitogenic at very low levels and inhibitory at higher levels. Both F and Al are present in finished drinking water where the chemical interaction of these two agents is well characterized. F and AI, given individually, accumulate preferentially in bone. In addition. in vivo studies have shown that F causes the co-accumulation of Al in bone. Thus, it was necessary to determine the interactive effect of these two agents on bone mitogenesis. Calvaria were obtained from neonatal CD-1 mice and cultured with various concentrations of F (0.05~19 ppm) as NaF, Al (2 ppb~2 ppm) as $AlCl_3$ , or F and Al for 3 days at $37^{\circ}C$ on a rotating roller drum. Alkaline phosphatase activity in calvaria and $\beta$-glucuronidase activity in culture medium were determined as a measures of bone turnover. Alkaline phosphatase activity in calvaria was significantly increased by F (0.05~2 ppm) treatment and $\beta$-glucuronidase activity was slightly increased in the culture medium of calvaria treated with 0.3 ppm Al. The combination of 19 ppm F and 0.3 ppm Al increased alkaline phosphatase activity in calvaria, but did not affect $\beta$-glucuronidase activity, suggesting the interactive effect of fluoride and aluminum on bone turnover.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.20 no.4
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• pp.381-388
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• 2013

Purpose: This study was done to investigate nurses' awareness of death and the meaning of life and identify factors influencing the meaning of life. Methods: Participants were 198 women nurses who had experienced the death of patients. They answered a self-administered questionnaire including demographics, awareness of death scale and meaning in life II scale. Data were analyzed using descriptive statistics, t-test, ANOVA, Scheff$\acute{e}$ test, and multiple regression (enter method). Results: The average score for awareness of death was $127.3{\pm}10.14$. This score is moderate not biased toward positive or negative. The average score for meaning of life was $130.3{\pm}9.42$ with 51% for loss steps, 45.5% for pursuit steps and 3.5% for discovery steps to the meaning of life. Factors affecting the meaning of life were religion, Buddhism (${\beta}=6.25$, p=.015) or Atheist (${\beta}=5.91$, p=.017), educational level, Master's or higher (${\beta}=16.22$, p=.003), work department, special department (${\beta}=3.49$, p=.017). Conclusions: Results of the study indicate a need to provide nursing programs that will promote nurses' spiritual and inner maturity.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.3
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• pp.420-427
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• 2015

It is necessary to understand the cellular uptake and cytotoxicity of food-grade delivery systems, such as ${\beta}$-lactoglobulin (${\beta}$-lg) nanoparticles, for the application of bioactive compounds to functional foods. The objectives of this study were to investigate the relationships between the physicochemical properties of ${\beta}$-lg nanoparticles, such as particle size and zeta-potential value, and their cellular uptakes and cytotoxicity in Caco-2 cells. Physicochemical properties of ${\beta}$-lg nanoparticles were evaluated using particle size analyzer. Flow cytometry and confocal laser scanning microscopy were used to investigate cellular uptake and cytotoxicity of ${\beta}$-lg nanoparticles. The ${\beta}$-lg nanoparticles with various particle sizes (98 to 192 nm) and zeta-potential values (-14.8 to -17.6 mV) were successfully formed. A decrease in heating temperature from $70^{\circ}C$ to $60^{\circ}C$ resulted in a decrease in the particle size and an increase in the zeta-potential value of ${\beta}$-lg nanoparticles. Non-cytotoxicity was observed in Caco-2 cells treated with ${\beta}$-lg nanoparticles. There was an increase in cellular uptake of ${\beta}$-lg nanoparticles with a decrease in particle size and an increase in zeta-potential value. Cellular uptake ${\beta}$-lg nanoparticles was negatively correlated with particle size and positively correlated with zeta-potential value. Therefore, these results suggest that the particle size and zeta-potential value of ${\beta}$-lg nanoparticles play an important role in the cellular uptake. The ${\beta}$-lg nanoparticles can be used as a delivery system in foods due to its high cellular uptake and non-cytotoxicity.

• Journal of the Korean Chemical Society
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• v.62 no.1
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• pp.14-18
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• 2018

The complexes $[Zn(L)(H_2O)_2]{\cdot}2NO_2$ (1) and $[Zn(L)(NO_3)_2]$ (2) (L = 3,14-dimethyl-2,6,13,17-tetraazatricyclo $[14,4,0^{1.18},0^{7.12}]$docosane) have been synthesized and structurally characterized. The compound 1 crystallizes in the monoclinic system $P2_1/c$ with a = 8.74650(10), b = 18.6880(3), c = $7.96680(10){\AA}$, ${\beta}=109.1920(10)^{\circ}$, $V=1229.84(3){\AA}^3$, Z = 2. The compound 2 crystallizes in the monoclinic system P1 with a = 8.1292(5), b = 8.9244(5), c = $9.1398(5){\AA}$, ${\alpha}=68.035(2)$, ${\beta}=70.109(2)$, ${\gamma}=75.649(3)^{\circ}$, $V=572.70(6){\AA}^3$, Z = 1. The crystal structures of the compounds 1 and 2 show a distorted octahedral coordination geometry around the zinc(II) ion, with four secondary amines and two oxygen atoms of the two water and two nitrate ligands at the axial position. The TGA behaviors of the complexes are significantly affected by the nature of the tetraaza macrocycle and the axial ligands.

• The Journal of Korean Physical Therapy
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• v.9 no.1
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• pp.103-115
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• 1997

The purpose of this study was to examine the amount of $\beta-endorphin$ and pain threshold according to time sequences in applying experimented TENS and MENS(pre treatment, posttreatment, 25 minutes after the treatment). This test was to aim at showing the effects of the experimental $\beta-endorphin$ and pain threshold applied to the randomly selected twenty healthy men in their twenties. The subjects were divided into two group(ten for TENS and the other ten MENS). The results obtained are as follows : 1. There was no significant difference in the $\beta-endorphin$ between TENS and HENS(p>0.05). There was no difference in the pain threshold at pretreatment and posttreatment(p>0.05), but there was some difference at 25 minutes after the treatment(p<0.05). 2. The group of TENS in the experimental $\beta-endorphin$ had the highest level of $14.40{\pm}3.098$ at posttreatment, but the level decreased a little according to time passed. And in the experimental pain threshold, the level went to the highest plateau of $2.92{\pm}0.483$ at 25 minutes after the treatment. 3. The group of MENS in the experimental $\beta-endorphin$ had the highest plateau of $14.20{\pm}3.967$ at posttreatment, but the level decreased a bit according to time passed also. And in the experimental pain threshold, the level went to the highest plateau of $2.49{\pm}0.617$ posttreatment. 4. There were some differences of the experimental $\beta-endorphin$ in TENS group at pretreatment and posttreatment(p<0.05). There were some differences in the experimental pain threshold between pretreatment and posttreatment as well as between pretreatment and 25 minutes after the treatment(p<0.05) MENS did not influence the experimental $\beta-endorphin$ and pain threshold. This experiment showed that TENS increased the levels of the experimental $\beta-endorphin$ at posttreatment and increased the levels of the experimental pain threshold untill 25 minutes after the treatment. Therefore, the time of sustaining pain in TENS group was longer than that of MENS group. Also, MENS showed that it increased each level of the experimental $\beta-endorphin$ and pain threshold, but these levels were not statistically meaningful.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.12
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• pp.1764-1770
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• 2012

This study was performed to investigate the changes of total and soluble ${\beta}$-glucan contents, purity, and physical characteristics of three heated barley varieties: Saessalbori (SSB), Saechalssalbori (SCSB), and Hinchalssalbori (HCSB). The barleys were heated at different temperatures of 110, 120, 130, 140 and $150^{\circ}C$ for 2 hours. The total ${\beta}$-glucan contents of raw SSB, SCSB, and HCSB were 8.40, 7.77 and 8.28%, and the soluble ${\beta}$-glucan contents were 4.79, 4.14, and 4.61%, respectively. After heating at $130^{\circ}C$, the total ${\beta}$-glucan contents increased to 11.59, 14.6, and 13.36%, as did the soluble ${\beta}$-glucan contents to 4.21, 7.96, and 7.23%, respectively. The purities of soluble ${\beta}$-glucan of the raw barleys were 35.11, 32.74 and 25.62%, but after heating at $150^{\circ}C$, it increased to 83.43, 91.02, and 88.01%, respectively. The molecular weight and viscosity of the ${\beta}$-glucan solution decreased with increasing heating temperature. The re-solubility of raw barley ${\beta}$-glucan was about 50%, but it was increased to 97% with increasing heating temperature. These results suggest that heating of ${\beta}$-glucan can improve the utilization of barley ${\beta}$-glucan.

• Journal of Ginseng Research
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• v.46 no.5
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• pp.666-674
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• 2022

Background: Ginsenosides and their metabolites have antidepressant-like effects, but the underlying mechanisms remain unclear. We previously identified 14-3-3 ζ as one of the target proteins of 20 (S)-protopanaxadiol (PPD), a fully deglycosylated ginsenoside metabolite. Methods: Corticosterone (CORT) was administered repeatedly to induce the depression model, and PPD was given concurrently. The tail suspension test (TST) and the forced swimming test (FST) were used for behavioral evaluation. All mice were sacrificed. Golgi-cox staining, GSK 3β activity assay, and Western blot analysis were performed. In vitro, the kinetic binding analysis with the Biolayer Interferometry (BLI) was used to determine the molecular interactions. Results: TST and FST both revealed that PPD reversed CORT-induced behavioral deficits. PPD also ameliorated the CORT-induced expression alterations of hippocampal Ser9 phosphorylated glycogen synthase kinase 3β (p-Ser9 GSK 3β), Ser133 phosphorylated cAMP response element-binding protein (p-Ser133 CREB), and brain-derived neurotrophic factor (BDNF). Moreover, PPD attenuated the CORT-induced increase in GSK 3β activity and decrease in dendritic spine density in the hippocampus. In vitro, 14-3-3 ζ protein specifically bound to p-Ser9 GSK 3β polypeptide. PPD promoted the binding and subsequently decreased GSK 3β activity. Conclusion: These findings demonstrated the antidepressant-like effects of PPD on the CORT-induced mouse depression model and indicated a possible target-based mechanism. The combination of PPD with the 14-3-3 ζ protein may promote the binding of 14-3-3 ζ to p-GSK 3β (Ser9) and enhance the inhibition of Ser9 phosphorylation on GSK 3β kinase activity, thereby activating the plasticity-related CREBeBDNF signaling pathway.

• Journal of Life Science
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• v.11 no.1
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• pp.57-64
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• 2001

The cDNA encoding human NeuAc ${\alpha}$2,3Gal$\beta$ 1,3GalNAc GalNac ${\alpha}$2,6-Sialyltransferase (hST6GalNac IV) was isolated by screening of human fetal liver cDNA library with a DNA probe generated from the cDNA sequence of mouse ST6Gal NAc IV (mkST6GalNAc IV). The cDNA sequence included an open reading frame coding for 302 amino acids, and comparative analysis of this cDNA with mST6GalNAc IV showed that each sequence of the predicted coding region contains 88% and 85% identifies in nucleotide and amino acid levels, respecively. The primary structure of this enzyme suggested a putative domain structure, like that in other glycosyltransferases, consisting of a short N-terminal cytoplamic domain, a transmembrane domain and a large C-terminal active domain. This enzyme expressed in COS-7 cells echibited transferase activity toward NeuAc ${\alpha}$2,3Gal$\beta$ 1,3GalNAc, fetuin and GM1b, although the activity toward the later is very low, no significant activity being detected toward Gal${\beta}$ 1,3Gal NAc or asialofetuin, the other glycoprotein substrates tested. The $^{14}$ C-sialylated residue of fetuin sialylated by this enzyem with CMP-[$^{14}$C]NeuAc was sensitive to treatment with ${\alpha}$2,8-specific sialidase of Vibrio cholerae but resistant to treatment with ${\alpha}$2,3-specific sialidase (NaNase I), and ${\alpha}$2,3- and ${\alpha}$2,8-specific sialidase of Newcastle disease virus. These results clearly indicated that the expressed enzyme is a type of GalNAc ${\alpha}$2,6-sialyltransferase like mST6GalNAc IV, which requires sialic acid residues linked to Gal${\beta}$1,3GalNAc-residues for its activity.

• Mass Spectrometry Letters
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• v.14 no.1
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• pp.9-23
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• 2023

Plants are able to produce a large number of diverse bioactive compounds. Solvent extraction is used for isolation of plant metabolites. The extract yield for plant metabolite extraction strongly depends on the nature of solvent. A review showed the methanol can yield more bioactive compounds. Drying of the sample material is also important for the extraction of plant material. The present study was carried out to analyze the phytocomponents of 5 different latex producing plants. The plants like Calotropis gigantea, Carica papaya, Nerium oleander, Ficus benghalensis and Plumeria alba leaves and latex. The GC-MS analysis of the metabolites revealed phytocomponents. Calotropis gigantea leaves showed 14 compounds and latex produced 5 compounds out of this 4,4,6A,6B,8A,11,11,14B-Octamethyl-1,4,4A,5,6,6A,6B,7,8,8A,9,10,11,12,12A,14,14A,14B-Octadeca-hydro-2 and 2R- Acetoxymethyl-1,3,3-trimethyl-4T-(3-Methyl-2-Buten-1-Yl)-1T-Cyclohexanol compound was present in both latex and leaf extraction. Beta. -carotene compound was present in both latex and leaf of Carica papaya. It was observed that Ficus benghalensis contained 2R-Acetoxymethyl-1,3,3-trimethyl-4T-(3-Methyl-2-Buten-1-Yl)-1T-Cyclohexanol was same in latex and leaf extraction.

• Journal of dental hygiene science
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• v.14 no.1
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• pp.51-58
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• 2014

The purpose of this study was to examine the symptom of dry mouth of workers, their subjective oral symptoms and factors affecting their oral health-related quality of life (Oral Health Impact Profile, OHIP-14) in an effort to provide information on the development of oral health promotion programs geared toward workers. The subjects in this study were the workers in five different companies in North Jeolla Province. After a survey was conducted from August 1 to September 20, 2012, the collected data were analyzed. The findings of the study were as follows: The office workers got 2.59 in oral dryness, and the sales associates got 1.82. The oral dryness of the former was severer than that of the latter (p<0.001). The irregular workers got 2.05 in OHIP-14, and the regular workers got 1.82 (p<0.001). Symptom of dry mouth had a positive correlation to OHIP-14 (r=0.456). OHIP-14 was positively correlated with age (r=0.209) and negatively with academic credential (r=-0.136). OHIP-14 was more significantly affected when their symptom of dry mouth was severer (${\beta}=0.383$, p<0.001), when they were older (${\beta}=0.221$, p<0.001) and when they felt they had oral diseases (${\beta}=0.146$, p<0.01). In conclusion, quality programs should be developed to promote the oral health of industrial workers in consideration of the influential factors for the oral health-related quality of life of the workers, and the government should pay more attention to related policy setting to improve the oral health of industrial workers.