• Korean Journal of Animal Reproduction
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• v.22 no.3
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• pp.287-292
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• 1998

The objective of this study was to test whether ZP drilling using a 1.48$\mu$m diode laser beam on mouse IVF embryos becomes effective the hatching and normal in vivo development, as a preliminary test for obtaining the additional proof that the 1.48$\mu$m diode laser could be used safely for human applications. The results obtained in this experiment were as follows: when the hatched rates of mouse embryos by laser ZP drilling according to the embryonic stage were examined until 72 hr (in case of blast tocyst: day 4 after IVF) or 120 hr (in case of 4-cell: day 2 after IVF) after treatment, the d data of laser drilled blastocysts (81.8%) was significantly higher than those of control (hatching blastocyst: day 4 after IVF) (54.2%) and laser drilled 4-cell embryos (45.5%) (p<0.05). When the effect of laser drilling on implantation rates following embryo transfer in day 3 synchronized pseudopregnant recipients was examined, the l laser drilled group (48.7%) was slightly higher than that of control group (43.6%). In addition, when the several pregnant mice delivered in two groups were analysed their chromosomal normality and tested reproductive ability, all p pups were presented normal chromosomal number (n=40) and showed normal growth and reproductive ability. Therefore, these results dem-onstrated that ZP drilling using a 1.48$\mu$m diode l laser can increase the embryo hatching and ind duce the normal pregnancy of mouse embryos.

• Journal of the Korean Institute of Telematics and Electronics A
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• v.32A no.2
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• pp.48-56
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• 1995

The operating characteristics, such as, the threshold gain, lasing frequency, and longitudinal intensity profile, etc., of 1.55$\mu$m DFB laser diode with index and/or gain grating structures and with one side AR-coated mirror have been analyzed. From this analysis, the optimum design parameters have been shown that ${\Delta}{\Omega}$ (the phase difference between index grating and gain grating) is 0 or ${\pi}$, (xL)$_{r}$=1~3 and (xL)$_{i}$=0.5~0.9. It has been also shown that the modal selectivity and intensity uniformity of the DFB lasers with .DELTA..OMEGA.=0 are ~1.2 times better thatn those of the DFB lasers with ${\Delta}{\Omega}$= ${\pi}$.