• Korean Journal of Food Science and Technology
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• v.40 no.3
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• pp.343-348
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• 2008

The ovomucoid (OM) of egg whites is recognized as a major allergen. Here, the allergenicity of OM in egg whites (EW) treated by chemical, enzymatic, and physiological methods were investigated by competitive inhibitory ELISA using human IgE antibody acquired from egg-allergic patients. Enzymatic hydrolysis, irradiation, and succinic anhydride treatments did not reduce the allergenicity of the OM effectively. Allergenicity was reduced to only 1/20 by deglycosylation with trifluoromethanesulfonic acid (TFMS). Heat treatment of the OM at $121^{\circ}C$ for 10 min reduced allergenicity to 1/100. Furthermore, NaOH (over 3%) treatment reduced allergenicity to 1/10,000, and the combinatory treatment of NaOH (over 0.3%) and heat ($70^{\circ}C$, 15 min) reduced it to less than 1/10,000, which was the most effective method. In this study, which analyzed treated EW using ELISA and patient-derived IgE, the OM allergenicity was nearly the same as its antigenicity according to ELISA using rabbit IgG. However, in the case of the TFMS-treated EW, the antigenicity was much lower than the allergenicity. These results suggest that the allergenicity of OM is slightly different from its antigenicity.

• Journal of the East Asian Society of Dietary Life
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• v.4 no.3
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• pp.59-65
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• 1994

Growth-stimulating effects of cow's milk was examined using Vero cell cultre. Medium containing whole cow's milk stimulated cell growth about the same degree as that containing fetal bovine serum. The growth-stimulating factor in cow's milk was purified using hydrophobic (phenyl-sepharose) and gel filtration (Sephadex G-100) column chromatographies. It appeared that the factor is a highly hydrophobic protein, since the major growth-stimulating activity was found in the fractions eluted with 50% ethylene glycol from the phenyl-sepharose column during the purification. The purified factor showed a single band on the polyacrylamide gel electrophoresis in the presence of 1% (w/v) SDS. The factor has been found to have a relatively high molecular weight in the range of about Mr=100,000-150,000. In the presence of the purified factor (5%, w/v) in the culture medium, the incorporation of [3H]-thymidine into the cells was increased approximately 2,400-fold over that in the presence of 5% (w/v) fetal bovine serum. It seems that the growth-stimulating factor purified in this study is one of the major growth factors in the cow's milk.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.6 no.1
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• pp.45-50
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• 1981

Infrasonic transducer made with dielectric materials, such as polytetrafluoroethyleme(PTFE)film. The experimental result obtained that the response is within $\pm$1.5dB from 0.1Hz to 7KHz, and that sensitivities of typical transducer are fixed -60dB. The time constant of the transducer at room temperature is over 60 years, and the activation energy of the value of 1.1eV at 343K acquired. This transducer can have application to high-quality communication system, seismological observation etc.

• Korean Journal of Ecology and Environment
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• v.35 no.2 s.98
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• pp.103-110
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• 2002

Seasonal changes of the phytoplankton community was investigated from July 2000 to April 2001 at the river bed lake, Jangjwa Lake in the Imjin River, which appeared to be the most natural condition. A total of phytoplankton were composed of 112 taxa, belonging to 5 divisions,83 species, 10 varieties,1 variety-form, 1 form and 17 unidentified species. Of those, the green algae and the diatoms were present during the investigation periods more frequently then the other taxa. The phytoplantton standing crops varied from $2,943{\times}10^3\;cells/l$ on April 2001 to $5,742{\times}10^3\;cells/l$ on January 2001. The phytoplankton standing crops had a continuous high value over $3,000{\times}10^3\;cells/l$ during the investigated periods and showed the highest value during winter period. The major dominant species were Aulacoseira granulata, Chlamydomonas nivalis v. kobayasii, Dinobryon sertularia, Mougeotia so. and Uroglenopsis americana. Among those, Chlamydomonas nivalis v. kobayasii was a major dominant species during winter period.

• Korean Journal of Crystallography
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• v.6 no.1
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• pp.43-48
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• 1995

The crystal structure of P-toluenesulfonanilide, C13H13NO2S is monoclinic, space group P21/c, a=8.777(1)Å, b=9.784(2)Å, c=15.139(2)Å, β=99.00(1)°, Z=4, V=1284.0(6)Å3, Dc=1.28g/cm33, λ(Mo-Kα)=0.71069Å, μ=2.3cm-1, F(000)=520, Temperature : 293±3K, R=0.038 for 711 Fo<3.0σ unique observed reflection. The structure was determuned by direct method and refined by full-matrix least squares refinement. Two benzene rings have the dihedral angle of 68.4°. Moleculs are accumulated according to the c axis with two fold screw and contacted by van der Walls force.

• Korean Journal of Food Science and Technology
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• v.38 no.3
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• pp.438-443
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• 2006

Antibacterial effects of six volatile essential oils against Vibrio sp. were evaluated. Volatile components of essential oil were analyzed by gas chromatography and gas chromatography mass spectrometry. Ginger oil treatment inhibited growth of V. parahaemolyticus by 22.5-85.7%. Main volatile compounds of ginger oil were ${\beta}-bisabolene$ (35.19%, peak area) and ${\beta}-sesquiphellandrene$ (12.22%). V. parahaemolyticus was completely inhibited at 1,000 ppm by treatment with mustard oil. Tolerances of V. vulnificus 01 and 02 were twice higher than that of V. parahaemolyticus. Main volatile compound of mustard oil was allyl isothiocyanate (92.55%). Garlic oil treatment of 1,000 ppm inhibited growths of V. parahaemolyticus, V. vulnificus 01, and V. vulnificus 02 by 22.8, 14.6, and 32.9%, respectively. Main volatile compounds of garlic oil were dimethyl sulfide (49.39%) and methyl 2-propenyl disulfide (10.09%). Growth of V. vulnificus 02 was inhibited by 60.6-80.3% via treatment with bud, leaf, and whole oil of clove. Antibacterial activity of whole clove oil on V. vulnificus 02 was stronger than those of ginger, mustard, and garlic oil. Main volatile compounds were eugenol (83.33%) and ${\beta}-caryophyllene$ (7.47%) in clove bud, eugenol (87.46%) and ${\beta}-caryophyllene$ (10.03%) in clove leaf, and eugenol (86.04%) and ${\beta}-caryophyllene$ (9.71%) in whole clove. These results revealed essential oils from spices could be used as potential agents to inhibit Vibrio sp.

• KSBB Journal
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• v.19 no.4
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• pp.315-320
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• 2004

We examined the culture conditions and degrading characteristics of styrene dimer (endocrine disrupter) using microorganism. The isolated microbe were consisted of 3 kinds of strain. The strains were identified to Pseudomonas sp. and Klebsiella pneumoniae by API 20E kit, but one was not identified. Single strain was not grown on the C-medium containing styrene dimer. However the complex strain YH3 could grow and we confirmed it by the broth color and O.D$_{660nm}$ (optical density 660 nm). The optimal culture conditions of complex strain YH3 were 35$^{\circ}C$, 1,000 ppm (v/v) of styrene dimer and pH 7.0, respectively. In tolerance test against the organic solvents, the complex strain YH3 could grow above log P=3.1, and could degrade ethyl benzene and 2,4-D, one kind of herbicide. As a result of TLC (Thin Layer Chromatography) analysis, we confirmed that the metabolite of styrene dimer was created by YH3 after 5th day, but not at control samples.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.616-625
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• 2016

This research was conducted to investigate the optimum level of nitrogen incorporated during formulation of root media as pre-plant fertilizer on the growth of plug seedlings of 'Bool-am No.3' Chinese cabbage. A root medium was formulated by blending peatmoss:coir dust:perlite at a ratio of 3.5:3.5:3.0 (v/v/v). The nitrogen was incorporated in the seven treatments at a rate of 0, 100, 250, 500, 750, 1,000, and $1,500mg{\cdot}L^{-1}$ during the root medium formulation. The concentrations of other essential nutrients except nitrogen were controlled to equal in all treatments. Then, the root medium was packed into 72-cell plug trays and seeds were sown. The growth measurements as well as tissue and soil solution analysis for nutrients were conducted 2 and 4 weeks after seed sowing. As seedlings grew, the pH in the extracted solution of all treatments tended to decrease. The decreases in the treatments of high N concentrations were more severe than those with low N, but the differences among treatments were not statistically significant. The differences of EC in extracted solution of root media among treatments were sizable until week 3, but the differences began to lessen and the EC decreased in all treatments after week 4. Growth of the aerial parts of plug seedlings at 2 weeks after sowing were highest in the $100mg{\cdot}L^{-1}$ and lowest in the $1,500mg{\cdot}L^{-1}$ treatments, but those at 4 weeks after sowing were highest in the $500mg{\cdot}L^{-1}$ and lowest in the $0mg{\cdot}L^{-1}$ treatments among all treatments tested. The tissue N content was highest and lowest in the treatments of 250 and $1,000mg{\cdot}L^{-1}$, respectively, when tissues were harvested at 4 weeks after sowing and analysed based on the dry weight of above-ground tissue. The contents of micronutrients were the highest in the 1,000 and $1,500mg{\cdot}L^{-1}$ treatments among all treatments. The results shown above indicate that the $250mg{\cdot}L^{-1}$ of pre-plant N and elevation of post-plant N concentration to above $100mg{\cdot}L^{-1}$ are suitable for raising plug seedlings of Chinese cabbage using inert media.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.48-48
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• 2018

본 연구는 경남 함양군 삼봉산의 관속식물을 조사한 후 식물상 현황과 특정식물종 및 귀화식물 등을 정리하였으며, 차후 식물상의 보호 관리에 있어 기초 자료로 활용하고자 실시하였다. 삼봉산은 전북 남원시 산내면 중황리, 인월면 상우리와 경남 함양군 마천면 구양리, 함양읍, 죽림리가 접하는 지역으로 남쪽으로는 국립공원 지리산의 연봉이 길게 늘어서 있고 북쪽으로는 광주대구 고속도로 건너 함양군의 백운산과 괘관산 등 1,000m가 넘는 산이 많은 지역내에 위치하고 있다. 삼봉산지역에 자생하는 관속식물은 총 80개과 182개속 254개 종이 생육하는 것으로 나타났다. 삼봉산 지역의 특정식물종은 식물구계학적 특정식물 V등급인 승마 Cimicifuga heracleifolia Kom.)와 IV등급인 도깨비부채(Rodgersia podophylla A.Gray), 점현호색 (Corydalis maculata B.U.Oh & Y.S.Kim), 솔붓꽃 (Iris ruthenica KerGawl.), 산복사나무 [Prunus davidiana (Carriere) Franch.] 등이 분포하는 것으로 확인되었다. 조사결과 가흥지역에서 환경부 멸종위기종은에 해당하는 분류군은 없었으며 특정식물종 V등급에 해당되는 승마와 IV등급에 해당되는 4개 분류군이 확인되었다. 국립공원지리산 근처의 1,000m이상의 산지임에도 전체적으로 50년 전부터이루어진 조림과 능선 바로 아래까지 경작지가 존재하는 등 식생의 파괴가 심하여 전체적인 관속식물의 다양성은 낮은 것으로 확인되었다.

• Journal of Environmental Science International
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• v.5 no.2
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• pp.179-185
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• 1996

This study was conducted to investigate the effects of environmental factors such as temperature, salinity, pH, and UV light on-the survival of life-threatening Vibrio vulnificus. In the temperature range of 15 to $25^{\circ}C$, the numbers of V. vulnificus incieased during the 6-day incubation, but outside this range, the survival of V. vulnificus was poor. Incubation between 1 and $10^{\circ}C$ showed that V. vulnifcts survived poorly below $10^{\circ}C$. At sal:nities between 5 and 25ppt, the numbers of V. vulnificus increased or remained unchanged for 6-day. At salinities above this range, the numbers of V. vulnificus decreased. The optimal pH range was 6.5 to 8.0 and outside this range, the survival ratio of V. vulnificus was small. At 15-and $25^{\circ}C$, UV radiation was bactericidal for cultures of V, vulnificus. The counts of V. vulnificus were reduced approximately 10, 000-fold after 2h of UV light treatment at both temperatures. Above results mowed 1ha't environmental factors were effective on the survival of V. vulniucus in the environment.