• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1275-1284
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• 2018

Objective: A 28-d trial was conducted to evaluate the effect of diets with different energy and lipase levels on performance, nutrient digestibility, serum profiles, gut health, and carcass quality in broilers. Methods: A total of 720 one-day-old male Ross 308 broilers ($45.4{\pm}0.5g$) were randomly assigned to one of the following four treatments: i) RET, reduced energy treatment (metabolizable energy = 2,950 and 3,100 kcal/kg for starter and finisher diet), ii) BDT, basal diet treatment (metabolizable energy = 3,050 and 3,200 kcal/kg for starter and finisher diet, iii) RET015, RET+0.15 g/kg lipase, and iv) RET03, RET+0.3 g/kg lipase. There were 10 replications (cages) per treatment with 18 birds per cage. Results: During d 1 to 14, broilers fed BDT, RET015, and RET03 diets had higher (p<0.05) body weight gain than those fed RET diet. During d 1 to 14, 15 to 28 and the overall experiment, feed conversion ratio in RET03 treatment was lower (p<0.05) compared with RET treatment. On d 14, the apparent total tract digestibility (ATTD) of dry matter (DM), ether extract (EE), and gross energy in RET03 treatment was higher (p<0.05) than those in RET treatment, while the ATTD of N was increased (p<0.05) by RET03 treatment. On d 28, broilers fed RET03 diet had higher (p<0.05) ATTD of DM than those fed RET and RET015 diets, while the ATTD of EE in BDT and RET03 treatments was increased (p<0.05) compared with RET and RET015 treatments. Broilers fed RET03 diet had higher villus height (VH) and VH:crypt depth (CD) ratio than those fed RET and BDT diets. The activity of pancreatic lipase in BDT and RET03 treatments was higher (p<0.05) than that in RET treatment. Conclusion: Taken together, lipase supplementation (3,000 U/kg feed) increased growth performance, nutrient digestibility, VH, VH:CD ratio and lipase activity, but decreased triglyceride, low-density lipoprotein cholesterol and the abdominal fat percentage in broilers fed reduced energy diet.

• Korean Journal of Plant Resources
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• v.10 no.4
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• pp.386-391
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• 1997

A study was carried out to investigate the variation of heading date and related yield components at Honam Agricultural Experiment Station in Korea. The treatments were consisted of five transplanting time: from May 5 to July 5 at 15-day intervals, and six cultivars: two early-maturing, two mid-maturing and two late-maturing cultivars. The results showed that variation of heading at different transplanting time was not significant at transplanting after June 5 in early-maturing cultivars, and days of heading from seeding for all tested cultivars were shorter in later transplanting times. The yield components such as numher of spikelcts, percentage of ripened grain and 1.000 grain weight were found to he great on May 5 in early-maturing cultivars and on June 5 in mid-and late-maturing cultivars. Yield was maximized at transplanting of May 20 in early maturing and on June 5 in mid-and late-maturing cultivars. When transplanting time was late, the yield difference hetween early maturing cultivars and late nldtunng ones showed 44 to 77kg/10a in the transplanting of June 20, and 26~30kg/10a in the transplanting of July 5 indicating that the late-maturing cultivars take more advantage than early-maturing ones.

• KSBB Journal
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• v.30 no.4
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• pp.148-154
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• 2015

Onion (Allium cepa) is one of the flavonoids-rich materials in human diet and onion peel, which is the onion by-products, contains over 20 times more quercetin than the flesh. In this study, to examine the anti-inflammatory effects of onion peel hot water extract (OPHWE), the cell viability, nitric oxide (NO), pro-inflammatory cytokines, such as interluekin-6 (IL-6), tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), and IL-$1{\beta}$, were measured using the murine macrophage cell line RAW 264.7 cells. The Balb/c mice were used for an in vivo acute toxicity test and ICR mice were used for measurement of inhibition effects of croton oil-induced mouse ear edema. As a result, NO levels decreased in a dose-dependent manner. The production of IL-6, TNF-${\alpha}$, and IL-$1{\beta}$ was suppressed by 38%, 41%, and 34% respectively, compared with that of the LPS only group, without any cytotoxicity. The edema formation in the ICR mouse ear was also reduced compared to that in control. Moreover, there were no mortalities occurred in mice administered 5,000 mg/kg body weight of OPHWE. These results suggest that OPHWE has considerable anti-inflammatory activities and can be regarded as a potent candidate material to treat inflammatory diseases.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.2
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• pp.241-246
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• 2004

To develop novel anti-aging peptides from the germinated black rice, we treated with bromelain, papain and Pronase E. And we investigated the effects of the germinated black rice peptide (GBRP) as anti-aging cosmetic ingredients, and compared with the non-germinated black rice protein (NBRP). We investigated the effects on in vitro inhibition of matrix-metalloprotease (MMP), proliferation of human skin fibroblasts, stimulation of collagen synthesis and expression of UVA-induced MMPs in human skin fibroblasts, UVA induced MMP-1 expression and collagen contents in human skin fibroblasts were analyzed by enzyme-linked immunosorbent assay (ELISA). As a result, the molecular weight distributions of GBRP and NBRP were determined by gel permeation chromatography to be approximately 900 and 10,000 daltons. GBRP increased skin cell proliferation about 40% and reduced UVA-induced MMP-1 expression about 50%. Also the collagen protein level of cells, which were cultured with GBRP, was increased about 25%. These results suggest that the geminated plant seed peptides can be novel anti-aging ingredients for cosmetics.

• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.133-148
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• 1987

Gibberellin(GA) 3-$\beta$ hydroxylation is very important for the shoot elogation in the higher plants, since only 3$\beta$-hydryoxylated GAs promote shoot elogation in several plants. Fluctuation of 3$\beta$-hydryoxylase activity was examined during seed maturation using two cultivars of , P. vulgaris, Kentucky Wonder (normal) and Masterpiece (dwarf). Very immature seeds of both cultivars contain high level of 3$\beta$-hydroxylase activity (per mg protein). Both cultivars showed maximum of enzyme activity (per seed) in the middle of their maturation process. Gibberellin 3$\beta$-hydroxylase catalyzing the hydroxylation of GA20 to GA1 was purified 313-fold from very early immature seeds of P. vulgaris. Crude soluble enzyme extracts were purified by 15% methanol precipitation, hydrophobic interaction chromatogrphy, DEAE ion exchange column chromatography and gel filtration HPLC. The 3$\beta$-hydroxylase activity was unstable and lost much of its activity duting the purification. The molecular weight of purified enzyme was extimated to be 42, 000 by gel filtration HPLC and SDS-PAGE. The enzyme exhibited maximum activity at pH 7.7. The Km values for [2.3-3H] GA20 and [2.3-3H]GA9 were 0.29 $\mu$M and 0.33 $\mu$M, respectively. The enzyme requires 2-oxoglutarate as a cosubstrate; the Km value for 2-oxoglutarate was 250 $\mu$M using 3H GA20 as a substrate. Fe2+ and ascorbate significantly activated the enzyme at all purification steps, while catalase and BSA activated the purified enzyme only. The enzyme was inhibited by divalent cations Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and Hg2+. Effects of several GAs and GA anaogues on the putrified 3$\beta$-hydroxylase were examined using [3H]GA9 and GA20 as a substrates. Among them, GA5, GA9, GA15, GA20 and GA44 inhibited the enzyme activity. [13C, 3H] GA20 was converted by the partially purified enzyme preparation to [13C, 3H]GA1, GA5 and GA6, which were identified by GC-MS, GA9 was converted only GA4, GA15 and GA44 were converted to GA37 and GA38, respectively. GA5 was epoxidized to GA6 by the preparation. This suggests that 3$\beta$-hydroxylation of GA20 and epoxidation of GA5 are catalyzed by the same enzyme in P, vulgaris.

• Journal of the Korean Society for Railway
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• v.19 no.2
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• pp.262-269
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• 2016

Subway station scales are determined by peak predictions. In this study, the purpose behind the installation of a subway is public transportation convenience and public interest, but economic validity is also important. By proving that the scale of the station is excessive with regard to the target station size for Seoul subway Line 5-8, a reasonable plan. can be sought. According to station installation standards, the area of the station under investigation here is out of the service levels by six stages (A~F), and it must be four or more levels (D). The Actual level for the B level is a two-step design. The Actual ratio for over- Peak predictions is only 17.8% on average. The results of measurements of the excess area and determination of the excessive costs were analyzed by subdividing the area and by calculating it based on the B level, finding that it is possible to provide benefits for customers only in the current design, with an area ratio of 16.3%. Given the weight, it was estimated that current conditions can meet the needs of only 18.6% of the current area. Simplifying the scale calculation method of the station, it is convenient, safe, and advantageous to move citizens only if the scale can be streamlined. Then, with a reduced initial investment, maintenance costs during the operation can be reduced.

• 월간산업보건
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• s.5
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• pp.4-15
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• 1988

In order to proffer the fundamental data for the better working environment and the effective establishment of hearing conservation program on workers exposed to industrial noise, author assessed noise levels on the 42 noisy processes among 84 manufactures of 9 industries and measured noise gearing loss by the type of industries on 3,104 workers at these noisy processes from March, 1986 to Februry, 1987. The results were summarized as follows: 1. The averge of A-weight sound level of 23 processes(54.8%) and the avergae of sound level at each octave band of 14 processes(33.3%) exceeded the permissible exposure limits in 8 hours per day. 2. The noise level was the highest in process of cocking of ship building(109.1dBA), and followed by plating of steel rolling(104.3dBA), rivet of manufacture of motor vehicles(102.5dBA), shot of ship building(98.5dBA), aciding(95.7BA) and steel tubing(95.0dBA) of steel rolling, weaving of textiles(95.0dBA). 3. The permissible exposure time for the average of sound level at each octave band was only 30 minutes in the process of cocking of ship building, plating of steel rolling and rivet of manufacture of motor vehicles. 4. As a result of audiometric examination in 3,104 workers, the rate of hearing loss over 50dB at 4,000Hz was 7.3%(227 workers) and the rate of hearing loss over 41dB at 60average method was 2.9%(89 workers). 5. The prevalence of occupational hearing loss in ship building and manufacture of motor vehicle was 5.2% and it was the highest among prevalence of these 9 industries. 6. As a result of this suvery, the noise control and gearing conservation program were required especially in the industry of ship building and manufacture of motor vehicle.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.26 no.4
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• pp.279-286
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• 1981

This study was conducted to find out the effect of wollastonite application in degraded saline soil condition on the growth of japonica rice variety. The variety, Palgweng was tested with 4 levels of wollastonite (200, 400, 600 and 400/200 kg/l0a) and two nitrogen levels (12 and 16 kg/l0a). The effect of wollastonite was higher in 16 than 12 kg of nitrogen application. The wollastonite application reduced rice leaf blast and dead left at lower nodal position, and increased percentage of effective tillers, percentage of ripened grains and 1, 000 grain weight. The SiO$_2$ content of upper leaf was higher than lower leaf in 200 kg of wollastonite application, but there was no difference under high wollastonite application (400 kg/l0a). Highly significant positive correlationships were obtained between SiO_2 content of plant at heading and percentage of ripened grains, (r=0.613) and SiO_2 content of flag leaf and percentage of ripened grains (r=0.407). Wollastonite application did not affect the development and degeneration of primary and secondary rachis branches of panicles, but it increased the number of spikelets. The highest grain yield was obtained with 16 kg of nitrogen and 600 kg of wollastonite.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.184-191
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• 1989

The effect of various supplementary light sources on the agronomic characteristics has been investigeted for generation acceleration of barley in green house with the condition of 15$^{\circ}C$ and 8 hrs. day light followed by each supplementary light sources including incandescent electric lamp (IN), Blue fluorescent lamp(BF). day-light fluorescent lamp(DF), Biolux lamp(BL), BL+BF, In+BF and IN+BL. Leaf number of main stem, leaf area, culm length, dry matter yield of shoot, number of grain per spike, grain yield per plant were most sharply decreased with the exposition of IN, while flag-leaf emergence on main stem and I, 000 grain weight were earlist and heavist respectively with the exposition of IN. In appeared to be best light source for shortening the days to heading, but IN + BF will be most desirable light source with considering both days to heading and adequate yield at the same time.

• Korean Journal of Organic Agriculture
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• v.10 no.2
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• pp.111-120
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• 2002

This study was conducted to investigate the effect of foliar application of Chitosan and seedling types on increasing the production of fall-growing potatoes(Solanum tuberosum) at the height of 250m from the sea in Jeju Island. The experiment main-plots consisted of four Chitosan applications, and subplots had three seedling types as cutting plug seedlings, mine-tuber plug seedling planting plot and seed potatoes planting plot. The foliar application of Chitosan of 2000ppm was carried out 0, 1, 3, 5 times at intervals of ten days after ten days of transplanting. Plant height and top dry weight were significantly more vigorous as three to five application of Chitosan. In the small tubers(under 80g), the number of tubers and tuber yield were relatively increased in the seed potatoes planting plot and mine-tuber plug seedling plot, but the large tubers(over 80g) was higher in the cutting plug seedling planting plot. T-N content in leaves was increased as the number of application of Chitosan was increased. A similar tendency was shown in K, P Ca, Mg and Na. The growth and tuber yield in cutting plug seedlings, mine-tuber plug seedling planting plot and seed potatoes planting plot were effectively increased as three to five foliar applications of Chitosan was increased.