• Title/Summary/Keyword: 03A05

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MHC Class II Allele Association in Korean Children With IgA Nephropathy and its Role as a Prognostic Factor (한국인 IgA 신병증 환아에서 MHC Class II유전자형과 예후와의 관계 분석)

  • Kim Pyung Kil;Yook Jinwon;Kim Ji Hong;Jang Yoon Soo;Shin Jeon-Soo;Choi In-Hong
    • Childhood Kidney Diseases
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    • v.4 no.1
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    • pp.33-39
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    • 2000
  • Purpose: Our study was designed to investigate the association of MHC Class II (DR, DQ) allele with IgA nephropathy and its significance as a prognostic factor for progression to ESRD Material and Methods: 69 children with IgA nephropathy with normal renal function(serum creatinine $\leq$ 1.5mg/dL) was classified as group A and 70 patients who received renal transplantation due to IgA nephropathy were selected as group B. The HLA-DQB1 and HLA-DRB1 alleles were studied by polymerase chain reaction using sequence specific primers. We have compared the difference in alleles between these two groups and with normal control and also examined any possible effect of the MHC class II genes on the histopathological severity and prognosis of IgAN. Results: Mean age was $8.8{\pm}2.9$ years in group A and $35.0{\pm}15.5$ years in group B. Male to female ratio was 2.8:1 in group A and 2.5:1 in group B. There was a significantly higher frequency of HLA-$DQB1^*03\;and\;DQB1^*05$ in Group B. The frequency of HLA-$DQB1^*0302\;and\;^*05031$ allele had increasing tendency in Group B(P<0.05). HLA-$DRB1^*03\;and\;^*05$ were more common in Group B(P<0.05). HLA-$DRB1^*04$ allele was the most common DR alleles in both group, but there was no statistical significance. There were no significant correlation with MHC class 13 genes on the hjstopathological severity in Group A. Conclusion: In conclusion, $HLA-DQB1^*0302\;and\;HLA-DQB1^*05031 $ allele seemed to be more common in transplanted patients compared to group with normal renal function suggesting that this allele is associated with poor prognosis in IgAN. However larger studies and follow up are required to confirm this due to uncharacterized heterogeneity in etiopathogenesis of IgA nephropathy and possibly one or more than one gene may exert influence in determining susceptibility to the diseases.

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Growth Associated Hormones Response and Fat Metabolism Change in Finishing Pigs Fed with n-Methyl-d, L-Aspartate

  • Xi, Gang;Xu, Zirong;Xiao, Ping
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.7
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    • pp.1026-1030
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    • 2002
  • A trial was conducted to investigate the effect of dietary NMA on several growth associated hormones and fat metabolism in finishing pigs. A total of 84 crossbred finishing pigs (average initial BW of $56{\pm}$0.37kg) were divided into 6 pens, 14 pigs per pen (7 gilts and 7 barrows per pen). 3 pens of pigs were fed with control diet (corn-soybean meal) and the others were fed control diet addition with 50 mg/kg NMA. During the trial, all pigs were given free access to feed and water. After 44 days trial, 8 pigs from each treatment (4 gilts and 4 barrows, weight similar to average group weight, $86.94{\pm}0.71kg$ for control group, and $90.55{\pm}1.51kg$ for NMA treated group) were sacrificed to collect the sample of the liver, longissimus muscle, subcutaneous fat (10th rib). The addition of NMA in diet increased the IGF-I, Insulin, T3, T4 levels in serum by 50.68% (p<0.05), 38.36% (p<0.05), 123.33% (p<0.01), 60.58% (p<0.03), respectively. Meanwhile, IGF-I level in the liver and the muscle were increased with 17.83% (p<0.03) and 26.00% (p<0.03) with addition of NMA. The data from subcutaneous fat (10th rib) analysis showed that supplement of 50 mg/kg NMA decreased the total activities of malic dehydrogenase (MDH) by 20.54% (p<0.05), glucose-6- phosphate dehydrogenase (G-6-DPH) by 16.97% (p<0.05), and decreased the specific activities of MDH and G-6-DPH by 37.46% (p<0.01) and 35.06% (p<0.01), respectively. The hormone sensitive lipase (HSL) total activity was increased by 25.00% (p<0.05) in NMA treated pigs. These results indicated that addition of 50 mg/kg NMA to diet can induce the endocrine great change in finishing pigs, furthermore, inhibit the fat synthesis through suppressing lipogenic enzymes and promote the fat degradation by elevating HSL activity in finishing pigs.

A Study of Professional Attitudes and Mental Health of Clinical Nurses (임상 간호사의 간호전문직 태도와 정신건강에 관한 연구)

  • 한경순;염순교;조주연;정연강
    • Journal of Korean Academy of Nursing
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    • v.27 no.2
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    • pp.364-376
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    • 1997
  • This study was made to analyse the nursing professional attitudes and mental health of clinical nurses in order to promote good mental health and positive attitudes. The 150 subjects obtained from 3 hospitals in Seoul completed a self-report questionnaire. Demographic data was collected in addition to data about nursing professional attitudes and mental health. The data was analyzed by SAS, t-test, scheffe's test, pearson's correlation and the GLM procedures. The results of the data analysis as follows : 1) Nursing professional attitudes were positive (MS=3.76) 2) Mental health was shown to be satisfactory (MS=0.65) 3) The relationship between nursing professional attitudes and mental health of the subjects were shown to be significant according to the professional education level with depression(r=-0.19, p=0.03/sup */), anxiety(r=-0.23, p=0.01/sup **/), hostility(r= -0.19, p=0.03/sup */), phobicanxiety(r=-0.18, p=0.04/sup */), but there was a significant difference according to the pursit of social profit with anxiety(r=-0.17, p=0.03/sup */). There was a significant difference according to autonomy with somatization(r=-0.23, p=0.01/sup **/), obsessive-compulsive(r=-0.22, p=0.01/sup **/), depression(r=-0.20, p=0.02/sup */), anxiety(r=-0.17, p=0.05/sup */). Finally, There was a significant difference according to ethics with hostility(r=-0.17, p=0.05/sup */). 4) According to nursing professional attitudes and demographic fators there was a significant difference in the professional position(t=2.01, p=0.05/sup */), work location(f=4.99, p=0.01/sup **/), length of employment (f=3.66, p=0.03/sup **/). 5) The nursing professional attitudes and occupational factors showed a positive correlation with the anticipation of length of employment (t=2.00, p=0.05/sup */). 6) According to Mental health and dmographic factors there was a significant correlation with the professional position(t=-2.21, p=0.03/sup */). 7) According to mental health and occupational factors there was a significant difference correlation. Based on the study results, the following recommendation are made : 1) Nursing education curriculum should be developed for the continuous promotion of mental of professional nurses. 2) Follow up study of identification of the variables which positively influence professional nurses attitudes.

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The determination of reference material for bone density by using bone phantom (골판톰을 이용한 골밀도측정 참조체의 결정)

  • Kim Jae-Duk
    • Imaging Science in Dentistry
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    • v.32 no.3
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    • pp.135-139
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    • 2002
  • Purpose: To determine the proper reference step wedge for digital Cu-Equivalent Image analyzing systems for measurement of bone density. Meterials and Methods : Radiograms of lumbar vertebrae phantom (1g/㎠) with 3 test copper step wedges of 0.03, 0.05 and, 0.1 mm thickness unit were taken and analyzed using NIH image software on a Macintosh personal computer. Measured densities of the lumbar areas in the Cu-Equivalent images made by utilizing 3 different copper stepwedges were compared with a known bone density. Results: The values of r2 for all copper equivalent images were over 0.99. The mean Cu-Eq value of lumbar in copper equivalent image made by a 0.1 mm copper stepwedge was 0.22 ± 0.06 mm and converted to hydroxyapatite density of 1.03 g/㎠. The stepwedges of 0.03 and 0.05 mm produced results having higher values than the actual known bone density. They did not show the blue and green color level that appeared in lumbar on color enhanced image. Conclusion : A copper stepwedge of adequate thickness and range of steps which can express the range of density of bone being measured should be used.

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Relationship between Peripheral Plasma Inhibin and Progesterone Concentrations in Sahiwal Cattle (Bos Indicus) and Murrah Buffaloes (Bubalus bubalis)

  • Mondal, S.;Prakash, B.S.;Palta, P.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.1
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    • pp.6-10
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    • 2003
  • The present study investigated peripheral plasma immunoreactive inhibin (ir-inhibin) concentrations in relation to the stage of oestrous cycle and progesterone concentrations in cycling Sahiwal cattle (Bos indicus) and Murrah buffaloes (Bubalus bubalis). Blood samples were collected once daily for thirty-two consecutive days from cattle and buffaloes (5 each) during winter months of January and February. Mean (${\pm}$S.E.M.) plasma ir-inhibin concentrations ranged from $0.40{\pm}0.01$ to $0.59{\pm}0.03ng/ml$ in cattle and from $0.29{\pm}0.03$ to $0.52{\pm}0.05ng/ml$ in buffaloes. In cattle, ir-inhibin concentrations increased from $0.47{\pm}0.07ng/ml$ on day -4 (day 0=day of oestrus) to reach a maximum value of $0.59{\pm}0.03ng/ml$ on day -2. Thereafter, ir-inhibin concentrations showed a decline to reach a low of $0.40{\pm}0.01ng/ml$ on day 11 of the oestrous cycle. In buffaloes, ir-inhibin concentrations increased from $0.38{\pm}0.04 ng/ml$ on day -4 to reach a maximum concentration of $0.52{\pm}0.05ng/ml$ on day -2. Ir-inhibin concentrations then declined to reach a low of $0.29{\pm}0.03ng/ml on day 9 of the cycle. In both cattle and buffaloes, ir-inhibin concentrations which were lowest ($0.43{\pm}0.02$ and $0.34{\pm}0.02ng/ml$, respectively) during the mid-luteal phase of the oestrous cycle increased (p<0.05) to $0.52{\pm}0.03$ and $0.44{\pm}0.04ng/ml$, respectively, during the late luteal phase, and then further to the highest value of $0.53{\pm}0.02$ and $0.49{\pm}0.04ng/ml$, respectively, during the perioestrus phase, following which these declined to $0.50{\pm}0.02$ and $0.39{\pm}0.03ng/ml$, respectively, during the early luteal phase. The variations in peripheral plasma ir-inhibin profile in both the species appear to be related to the changes in characteristics of follicular populations during the oestrous cycle. Peripheral plasma ir-inhibin concentrations were negatively correlated with progesterone concentrations in cattle (r=-0.51, p<0.01) and buffaloes (r=-0.30, p<0.01) indicating that the corpus luteum is not a source of peripheral ir-inhibin in these species.

Chemical Composition of Leaves, Stem bark and Fruit Essential Oil from Premna foetida Linn

  • UMARU, Isaac John;AHMED, Maryam Usman;HABIBU, Bilyaminu;EMOCHONE, Yohanna Roy
    • The Korean Journal of Food & Health Convergence
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    • v.7 no.6
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    • pp.1-8
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    • 2021
  • Premna foetida is a woody plant with short and twisted trunk. P. foetida is a scandent, erect shrub or small tree, thorny on the trunk and large branches. Leaves are opposite or whorled and entire or serrate. Premna foetida is a wild plant locally known as "Daun Sebuas". P. foetida is used for it nutritive and as traditional treatment. The fruit and leaves of P. foetida are prepared for salad. The study aimed at the hydrodistillation and antioxidant activity of leaves, stem-bark and fruits essential oil from Premna foetida Linn, they were analysed by capillary GC and GC-MS. Ninety eight compounds representing 81.68±0.02, 37.31±0.05 and 93.45±0.03 of the isolates of leaves, stem-bark and fruits respectively were identified, the most abundant were α -Duprezianene (77.27±0.03, leaves, α-Gurjunene (36.06±0.05) fruits and Hinesol acetate (77.19±0.03) stem-bark. Components among which sesquiterpenoids dominated. The total volatiles were assayed for antioxidant potentials using 2, 2-diphenyl-1-picrylhydrazyl (DPPH). The total volatiles showed strong activity with IC50 of 11.74±0.82㎍/ml, 9.63±0.34 ㎍/ml and 49.73±1.12 ㎍/ml for leaves fruits and stem-bark respectively.

Combination of a new ultrasonic tip with rotary systems for the preparation of flattened root canals

  • Karina Ines Medina Carita Tavares ;Jader Camilo Pinto ;Airton Oliveira Santos-Junior ;Fernanda Ferrari Esteves Torres ;Juliane Maria Guerreiro-Tanomaru ;Mario Tanomaru-Filho
    • Restorative Dentistry and Endodontics
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    • v.46 no.4
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    • pp.56.1-56.11
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    • 2021
  • Objectives: This study evaluated 2 nickel-titanium rotary systems and a complementary protocol with an ultrasonic tip and a small-diameter instrument in flattened root canals. Materials and Methods: Thirty-two human maxillary second premolars with flattened canals (buccolingual diameter ≥4 times larger than the mesiodistal diameter) at 9 mm from the radiographic apex were selected. The root canals were prepared by ProDesign Logic (PDL) 30/0.01 and 30/0.05 or Hyflex EDM (HEDM) 10/0.05 and 25/0.08 (n = 16), followed by application of the Flatsonic ultrasonic tip in the cervical and middle thirds and a PDL 25/0.03 file in the apical third (FPDL). The teeth were scanned using micro-computed tomography before and after the procedures. The percentage of volume increase, debris, and uninstrumented surface area were analyzed using the Kruskal-Wallis, Dunn, Wilcoxon, analysis of variance/Tukey, and paired and unpaired t-tests (α = 0.05). Results: No significant difference was found in the volume increase and uninstrumented surface area between PDL and HEDM (p > 0.05). PDL had a higher percentage of debris than HEDM in the middle and apical thirds (p < 0.05). The FPDL protocol resulted in less debris and uninstrumented surface area for PDL and HEDM (p < 0.05). This protocol, with HEDM, reduced debris in the middle and apical thirds and uninstrumented surface area in the apical third (p < 0.05). Conclusions: High percentages of debris and uninstrumented surface area were observed after preparation of flattened root canals. The HEDM, Flatsonic tip, and 25/0.03 instrument protocol enhanced cleaning in flattened root canals.