• 한국미생물·생명공학회지
• /
• 제28권5호
• /
• pp.264-269
• /
• 2000

This study is that of providing a fairly practical practival guide to the use of natural pigment in food industry. A strain isolated from marine resources was carried out the production of red pigment. The pigment showed UV absorption maxima at 520 and 550 nm. The color intensity in aqueous solution was fairly stable in the ranges of pH 5~8. The strain KS-97 produced a maximum yield of red pigment at$ 25^{\circ}C$ for 72 hrs with pH 7.0. The strain KS-97 was iden-tified a Bacillus sp. based on morphological and biochemical characterization such as a rod from, motility, spore for-mation, Gram positive and catalase production. The production of red pigment indicated that the strain Ks-97 utilized at thigh concentration of colloidal chitin as carbon sources obtained maximum yield of red pigment at $25^{\circ}C$ for 72 hrs. The highest production of red pigment was observed with cultivation in medium containing 20% colloi-dal chitin, 2.5g polypeptone, 2.5g yeast extract, 1.0g $KH_2$$PO_4$, 0.01g $MgSO_4$.$6H_2$O, 0.01g $ZnSO_4$, 0.01 g $MnSO_4$(per 1).

• Physical Therapy Rehabilitation Science
• /
• 제12권1호
• /
• pp.72-79
• /
• 2023

Objective: Despite known associations between negative body image and health declines in chronic pain patients, few studies have examined longitudinal associations between psychological stress and perceived hand deformities. This study examined whether psychological distress was associated with hand deformities 4 years later and if rural and urban adults differed in the association. Design: A community-based cohort study. Methods: Community dwelling adults (mean age=51.97, 52.3% women) in a rural (n=2968) and urban area (n=2784) provided demographic data at baseline and, in the 4-year follow-up, responded to questionnaires about psychological distress. Perceived hand deformities were assessed at the 8-year follow-up. Linear regression was conducted to examine the effects of psychological distress on hand deformities and moderation by residential areas. Results: The perceived hand deformities were more likely among those with severe psychological stress, hand osteoarthritis, or any chronic disease condition (p<0.01) but less likely among those with younger age, higher education, or income (p<0.01). The regression results showed that psychological distress predicted more perceived hand deformities 4 years later even after adjusting for demographic and health covariates (p<0.01). The residential areas did not significantly moderate the association between distress and hand deformities. Conclusions: This study suggected that psychological distress may trigger later perception of hand deformities in both urband and rural adults. The findings indicate that stress management interventions that are customizable to regional contexts may be effective at preventing negative body image related health problems of community-dewelling adults.

• 대한한방내과학회지
• /
• 제27권2호
• /
• pp.316-326
• /
• 2006

Backgrounds & Objects: The aim of this study was to investigate the radioprotective effect of Shengmai-san(SMS), a herbal medicine, on mice jejunal crypt cell survival and Apoptosis. Methods: Mice were devided into 4 groups according to radiation dose and SMS treatment: Normal was the group without irradiation. Control was the group treated with D.W before 10 Gy irradiation. SMS 2.9 was sample group treated with 2.9 mg/10 g of SMS extract before 10 Gy irradiation and SMS 29 was sample group treated with 29 mg/10 g of SMS extract before 10 Gy irradiation. And Each group were sacrificedat 24 hours and 72 hours after irradiation. To analyze the crypt survival, hematoxylin-eosin staining was used and to analyze the apoptosis, the TUNEL assay was done. Results: 1. From the microcolony survival assay, the SMS 2.9 and SMS 29 showed the radioprotective effect with a statistical significance compared to the control group at 24 hr (P < 0.01) and 72 hr (p < 0.001) after 10 Gy irradiatien. And the differences of radioprotective effect between SMS 2.9 and SMS 29 were net significant. 2. The results of the TUNEL assay showed that the apoptotic index in SMS 2.9 and SMS 29 was significantly decreased, as compared to the control group at both 24 hr ( p < 0.01) and 72 hr (SMS 2.9 : p < 0.001. SMS 29 : P < 0.01) after 10Gy irradiation And the differences of between SMS 2.9 and SMS 29 were not significant. Conclusions: It could be suggested that the Shengmai-san has a prominent Protective effect in mice intestines against the radiation damage. And the radieprotective effect seems to be related to inhibition of the apoptosis.

• 대한수의학회지
• /
• 제27권1호
• /
• pp.9-17
• /
• 1987

The present study has been carried out to elucidate the antiestrogenic effects of tamoxifen in immature rat uterus. The content of DNA and protein and uterine wet weight were measured after the injections of $5{\mu}g$ of estradiol-$17{\beta}$, $50{\mu}g$ of tamoxifen, a combination of both, or vehicle only subcutaneously. The results obtained were summarized as follows: 1. DNA content in uterus was increased at 48 hours after estradiol-$17{\beta}$ or tamoxifen injection (p<0.01). 2. The increament rate of uterine DNA content was significantly (p<0.01) lower in tamoxifen treated group than that in estradiol-$17{\beta}$ treated group. 3. Antiestrogenic effect of tamoxifen on protein content in uterus was apparent at 72 hours after simultaneous administration of both drugs. 4. The uterine wet weight was started to increase at three hours after the injection of estradiol-$17{\beta}$ or tamoxifen. 5. While estradiol-$17{\beta}$ increased steadily uterine wet weight up to 138.5mg at 72 hours after the injection, but tamoxifen failed to increase it after 48 hours. Tamoxifen inhibited significantly (p<0.01) the effect of estradiol-$17{\beta}$ on it thereafter.

• 디지털융복합연구
• /
• 제12권2호
• /
• pp.409-415
• /
• 2014

본 연구는 일 지역 간호대학생의 임상실습 스트레스와 그에 대한 스트레스 대처의 관련성을 알아보기 위한 것이다. 한국의 C 도시에 위치한 2개 대학의 간호학생을 대상으로 하였으며, 자료수집은 연구참여에 동의한 사람 160명을 대상으로 2012년 3월부터 9월까지 구조화된 설문지를 이용하여 수행하였다. 수집된 자료는 SPSS 18.0을 이용하여 분석하였고, 서술통계, 피어슨 상관분석 등을 이용하였다. 연구결과 임상실습 스트레스 점수는 평균 $2.72{\pm}6.2$였으며, 스트레스 대처점수는 $2.60{\pm}0.37$ 이었다. 스트레스 대처양상 중 희망적 사고는 오리엔테이션(r=0.32, p<0.01), 집담회의(r=0.52, p<0.01), 과제하기(r=0.29, p<0.01)시의 스트레스가 발현시에 관련성이 높았고, 임상실습 시험시의 스트레스는 문제집중대처양상과 관련성이 높았다(r=0.31, p<0.05). 간호사 대하기(r=0.31, p<0.01), 또는 기타 보건전문인과 대인관계하기(r=0.35, p<0.01)시의 스트레스는 긍정적 관점으로 대처하기 양상과 관련성이 높았다. 학생들은 긍정적이고 활동적인 대처양상을 보여주었으며, 이는 임상현장에서 경험하는 스트레스를 효과적으로 다룰 수 있는 충분한 능력을 향상시키는데 좋은 경험이 될 것이다.

• 대한수의학회지
• /
• 제25권1호
• /
• pp.41-48
• /
• 1985

In the present studies, the effects of dietary high-potassium and high-urea on the metabolism of magnesium, calcium and potassium were checked as an aid to clarify the pathogenesis of hypomagnesaemia(so-called grass tetany) in ruminant. A total of 5 Korean native female goats kept in metabolic cage were received high-potassium(Mg: 0.25%, Ca: 0.94%, K: 5.41%), high-urea(Mg: 0.25%, Ca: 0.94%, K: 0.72%) or control(Mg: 0.25%, Ca: 0.94, K: 0.72%) ration for 15 or 21 days. Daily intakes, fecal and urinary excretions and serum concentrations of magnesium, calcium and potassium were measured with an atomic absorption spectrophotometer. The results obtained were summarized as follows: In high-potassium group, absorption, urinary excretion and serum concentration of magnesium were significantly (p<0.01, p<0.01 and p<0.05 respectively) decreased compared with the control group. On the other hand, the body retention was significantly (p<0.01) increased. However, no clinical symptom of hypomagnesaemic tetany was observable throughout the experimental period. No significant effects on the metabolism and seurm content of calcium were detected in the high-potassium group. In the high-potassium group, significant increase in absorption (p<0.01), urinary excretion (p<0.05) and body retention (p<0.01) of potassium were recognized. However, no significant difference in the concentration of serum was observable between the two groups. In high-urea group, no significant difference in the metabolism of magnesium and potassium or in the serum content were recognized compared with the control group. In high-urea group, tthe absorption, urinary excretion, body retention and serum content of calcium were decreased compared with the control group.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권6호
• /
• pp.2491-2494
• /
• 2014

Objective: To explore the mechanism and significance of tumor angiogenesis by observing changes of microvessel density (MVD) and expression of vascular endothelial growth factor (VEGF) in residual tumor tissues after cryoablation. Materials and Methods: A total of 18 nude mice xenograft models with transplanted lung adenocarcinoma cell line A549 were established and randomly divided into 3 groups when the maximum diameter of tumor reached 1 cm: control, cisplatin (DDP) and cryoablation. The nude mice were sacrificed after 21-d cryoablation to obtain the tumor tissues. Then immunohistochemistry was applied to determine MVD and the expression of VEGF in tumor tissues. Results: The tumor volumes of control group, DDP group and cryoablation group were $1.48{\pm}0.14cm^3$, $1.03{\pm}0.12cm^3$ and $0.99{\pm}0.06cm^3$ respectively and the differences were significant (P<0.01), whereas MVD values were $21.1{\pm}0.86$, $24.7{\pm}0.72$ and $29.2{\pm}0.96$ (P<0.01) and the positive expression rates of VEGF were $36.2{\pm}1.72%$, $39.0{\pm}1.79%$ and $50.8{\pm}2.14%$ (P<0.01), respectively, showing that MVD was proportional to the positive expression of VEGF (r=0.928, P<0.01). Conclusions: Cryoablation can effectively inhibit tumor growth, but tumor angiogenesis significantly increases in residual tumors, with high expression of VEGF playing an important role in the residual tumor angiogenesis.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권10호
• /
• pp.1447-1455
• /
• 2011

Sixty four, 30-week-old, Lohmann Brown-Lite laying hens were randomly allocated to one of four treatments with eight replicates per treatment and two hens per replicate for a 10 week study. The control diet was a high energy (11.81 MJ/kg) diet and the moderate energy (11.39 MJ/kg) diets were formulated dropping the level of animal fat. The three moderate energy diets were fed either unsupplemented (0.0%) or supplemented with 0.05 or 0.10% glycine. There were no significant differences (p>0.05) in hen-day egg production, egg weight, feed intake or feed conversion between birds fed the unsupplemented moderate energy and high energy diets. Significant differences were detected concerning egg components and quality measurements as assessed by albumen percentage (p = 0.02), yolk weight (p = 0.02), yolk percentage (p<0.01), yolk to albumen ratio (p<0.01) and yolk color (p = 0.01) between birds fed the unsupplemented moderate and high energy diets. Glycine supplementation of the moderate energy diet linearly increased (p<0.01) egg weight and feed intake with no significant (p>0.05) effects on egg production or feed conversion. Glycine supplementation significantly increased egg content (p<0.01), albumen weight (p<0.01) and percentage (p<0.01) as well as yolk weight (p<0.01) while yolk percentage (p = 0.04), yolk to albumen ratio (p = 0.01) and egg shell percentage (p<0.01) were linearly decreased. Supplementation with glycine produced a tendency (p = 0.09) towards an increase in the percentage of large eggs (63-72.9 g) produced with a concomitant decrease in the percentage of small (below 53 g) eggs (p = 0.09). The overall results of this study indicate that glycine supplementation of laying hen rations has the potential to increase egg production and weight. These increases appeared to be mediated through increases in feed intake and the ileal digestibility of fat and energy.

• Asian Pacific Journal of Cancer Prevention
• /
• 제13권3호
• /
• pp.915-921
• /
• 2012

Background: Previous studies investigating the association between TP53 Arg72Pro polymorphism and gastric cancer (GC) risk in Asian population have reported controversial results. Thus, a meta-analysis was performed. Methods: A comprehensive literature search was conducted and 17 case-control studies were finally included, involving a total of 5,990 GC cases and 6,812 controls. Subgroup analyses were performed by the sample size. Results: Meta-analysis of all 17 studies showed variant genotypes of TP53 Arg72Pro to be associated with an elevated GC risk in three genetic comparison models ($OR_{Pro\;vs.\;Arg}$=1.13, 95%CI 1.03-1.25, $P_{OR}$=0.01; $OR_{Homozygote\;comparison\;model}$=1.33, 95%CI 1.07-1.64, $P_{OR}$=0.009; $OR_{Dominant\;genetic\;model}$=1.13, 95%CI 1.05-1.22, $P_{OR}$=0.002). Besides, a more obvious association was observed after the heterogeneity was decreased (all P values less than 0.001). This association was further identified by both subgroup and sensitivity analyses. Conclusions: This meta-analysis suggests the Pro variant of TP53 Arg72Pro contributes to gastric cancer risk in Asians.

• 한국콘텐츠학회논문지
• /
• 제12권3호
• /
• pp.260-267
• /
• 2012

본 연구는 연구대상자가 일반학교 학생이 아닌 특성화학교의 학생을 대상으로 구강보건의식행태에 관해 조사한 결과 아래와 같다. 1. 학생들의 잇솔질 시기에 대해 살펴본 결과, 저녁식사 후에 잇솔질을 하는 학생이 29.5%로 가장 많았고, 우식예방을 위한 설탕함유 음식 섭취제한 여부에 관한 결과, 그렇지 않은 편이라는 학생이 53.3%로 가장 많았으며, 구강보건지식에 대한 정보 습득 경로에 관한 결과, 이웃과 가족을 통해 지식을 얻는다는 학생이 72.0%로 가장 많았다. 2. 학생들의 1일 평균 잇솔질 횟수에 대해 살펴본 결과, 3회 실시하는 학생이 45.2%로 가장 많았으며, 학년(p<0.01), 성별(p<0.01), 등하교 방법(p<0.05), 경제적 상태(p<0.01)에 따라 유의한 차이가 있었다. 3. 학생들의 보조구강위생용품 사용여부에 대해 살펴본 결과, 사용한다는 학생이 64.5%로 가장 높았으며, 학년이 높은 고등학교에서 높았으며(p<0.01), 경제적인 상태(p<0.01)에 따라 유의한 차이가 있었다. 4. 학생들의 과자 등 간식 섭취횟수에 대해 살펴본 결과, 하루에 2-3회 37.7%, 1번 34.6%로 비슷하게 나타났으며, 경제적 상태(p<0.01)에 따라 유의한 차이가 있었으며, 음료 섭취횟수의 결과는 마시지 않음 38.0%, 1번 37.7%로 비슷하게 나타났고, 학년(p<0.01), 경제적 수준(p<0.05)에 따라 음료섭취의 횟수가 통계적으로 유의한 차이를 보였다.