• Journal of Preventive Medicine and Public Health
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• 제17권1호
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• pp.269-279
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• 1984

The optimum conditions for measuring cadmium content of less than 0.2ppm by flame atomic absorption spectrophotometry were investigated. The cadmium in urine was extracted by APDC-MIBK for the analysis by atomic absorption spectrophotometry after ashing them by a wet method. 1. Optimum conditions by APDC-MIBK and DDTC-MIBK extractions. The acidic aqueous solution was prepared with appropriate amount of 0.IN nitric acid, 5ml of 25% (W/V) sodium potasstum tartarate, 10ml of saturated ammonium sulfate, and 2ml of 2% APDC(or 1 ml of 5% DDTC) chelating agent. The total volume of solution was adjusted to 55 ml and pH to $2{\sim}10$ (or$7{\sim}10$). The aqueous solution was extracted with 10ml MIBK. Concentration of Triton X-100 did not effect the absorbance for APDC-MIBK extraction of cadmium, but absorbance decreased as the concentration increased for DDTC-MIBK extraction. The sensitivity and detection limits for the cadmium determination from APDC-MIBK extraction were 0.0038ppm and 0.0102, 0.0022ppm and 0.0116 for DDTC-MIBK, and 0.0132ppm and 0.0034 for 0.1N nitric acid. APDC-MIBK and DDTC-MIBK extractions were 3 times higher than 0.1N nitric acid for the sensitivity. 2. Excretion of cadmium in 24-hour urine by APDC-MIBK extraction. Determination of cadmium in urine by atomic absorption spectrophotometry of A.A. (Cd=2 mA) mode and B.C. (Cd=4 mA) mode and B.C. (Cd=4mA, $D_2=20mA$) mode showed some difference (p<0.05). The difference of cadmium determination and recovery according to method of standard additions and standard calibration curve method in urine was not significant (p>0.05, $93.48{\pm}11.78%,\;94.83{\pm}22.00%$). Excretion of cadmium in 24-hour urine collection from normal person and variance analysis within measurement variation was not significant (p>0.05), but between interindividual was significant (0.05). Determination of cadmium content by two different methods of flame atomic absorption spectrophotometry and dithizone colorimetry showed that the results from the two methods can be described by a regression line with a good correlation (y=1.0153x-0.2927, x=Cd by D.C., y=Cd by A.A.S., $r=0.8651^*$, p<0.01).

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제32권1호
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• pp.8-18
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• 2006

Purpose: The purpose of this study was to verify that the expressions of angiogenin, transforming growth factor-beta(TGF-${\beta}$), vascular endothelial growth factor(VEGF), human apurinic/apyrimidinic endonuclease(APEX) and tumor necrosis factor-alpha(TNF-${\alpha}$) were associated with the tumorigenesis of the oral squamous cell carcinoma(OSCC). Materials and Methods: Fifty-one samples of OSCC and fifteen normal oral mucosae were obtained to analyze the expression levels of above five factors. mRNA expressions were quantified by the quantitative competitive PCR(QC-PCR) method. After 2% agarose gel electrophoresis stained with ethidium bromide, the concentration of mRNA was calculated by a digital image analysis system. The expression levels of angiogenin, TGF-${\beta}$, VEGF, APEX and TNF-${\alpha}$ were compared by unpaired Student's t-tests between cancer and normal tissues. We analyzed statistically to find the cut-off values that would be useful as diagnostic markers, and the linear regression analysis between every two factors of these five factors by SAS system. Results: All of these five factors (angiogenin: P<0.0037, TGF-${\beta}$: P<0.0001, VEGF: P<0.0102, APEX: P<0.0023, TNF-${\alpha}$: P<0.0074) were significantly correlated with OSCC. In the analysis to find the cut-off values for the diagnosis, we could not find any value that had a reasonable sensitivity and specificity. In the linear regression analysis, there were correlations between angiogenin and TNF-${\alpha}$, TGF-${\beta}$ and VEGF, TGF-${\beta}$ and APEX, TGF-${\beta}$ and TNF-${\alpha}$, VEGF and APEX, VEGF and TNF-${\alpha}$, APEX and TNF-${\alpha}$. Conclusion: Our results suggest that not only angiogenin, TGF-${\beta}$, VEGF, APEX and TNF-${\alpha}$ are significantly associated with the tumorigenesis, but also the close relationship between these factors might enhance the tumorigenesis of OSCC. We can not find clinical availability for diagnosis.

• 한국수산과학회지
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• 제55권2호
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• pp.102-110
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• 2022

This study was conducted to optimize the processing process for the oyster Crassostrea gigas gratin with cream sauce (OG-CS). The optimum concentration of added milk for oyster extract with milk (OE-M) was 35.0% based on the frozen-boiled oyster (F-BO), as suggested by the results of sensory evaluation. Response surface methodology was performed with whipping cream (WC)/[OE-M+mixed powder (garlic powder:onion powder=1:1) (MP)] (X₁) and OE-M/MP (X₂) as independent variables and viscosity (Y₁), amino acid nitrogen (Y₂), and overall acceptance for sensory evaluation (Y₃) as dependent variables. The optimal proportions were 74.55% of WC, 20.25% of OE-M, and 5.2% of MP, and the predicted multiple response optimal values for the dependent variables were 3,735.6 cP of Y₁, 197.0 mg/100 g of Y₂, and 6.2 score of Y₃. Under optimal conditions, the experimental values for Y₁, Y₂, and Y₃ were 3,711.9±30.0 cP, 198.1±1.9 mg/100 g, and 6.3±0.5 score, respectively, which were not significantly different from the predicted values (P>0.05). Further, the results of sensory evaluation suggested that the optimum concentration of macaroni:cheese (1:2) to be 46.2% based on the F-BO. The OG-CS prepared under these optimal conditions was superior to the commercial seafood gratin in overall acceptance.

• 마이크로전자및패키징학회지
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• 제30권3호
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• pp.102-110
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• 2023

본 연구에서는 플렉서블 레이저 투과 용접 (flexible laser transmission welding, f-LTW)을 이용한 박형 기판의 사면 접합 (scarf welding) 공정을 개발하였다. 플렉서블 응용을 위해 경사면의 기울기에 따른 인장 강도의 거동을 조사하였다. 박형 기판으로써 100 ㎛ 이하 두께의 플라스틱 기판이 사용되었으며, 사면 접합을 위해서 기판의 말단에 경사면을 형성하는 지그 장치를 개발하였다. 플렉서블 고분자 기판에 대한 경사면 맞대기 접합을 개발함으로써 공정 후 접합부 두께가 증가하지 않는 유연 접합 기술 개발에 성공하였다. 단축 인장시험을 통해 접합부의 인장 강도를 평가하였으며, 그 결과 경사면의 기울기가 완만할수록 인장 강도가 증가함을 확인하였다. 경사각에 따른 접합 계면에서의 응력 분석을 수행하여 접합 구조 설계 인자를 규명하였다. 본 결과는 동일한 공정 조건에서 접합부의 형상에 따라서 인장 강도가 크게 달라질 수 있음을 시사하므로 접합 공정에서 접합부 형상을 고려하는 것에 대한 중요성을 확인할 수 있다.

• 대한영상의학회지
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• 제85권2호
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• pp.437-444
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• 2024

고유신장과 이식신장 모두에 신세포암종이 발생하는 경우는 매우 드물며, 소수의 증례만이 영문 문헌에서 보고되었다. Xp11.2전위/전사인자E3 (이하 TFE3)-재배열 신세포암종은 성인인구에서 드문 아형이다. 신장이식을 받은 어린이에서 TFE3-재배열 신세포암종이 소수의 증례로 보고되었으나, 어른에서 신장이식 후 TFE3-재배열 신세포암종이 보고된 증례는 없다. 저자들은 이식신장에 유두모양 신세포암종이, 고유신장에 TFE3-재배열 신세포암종이 있던 드문 증례를 영상 소견과 함께 보고하고자 한다. 고유신장에 생긴 TFE3-재배열 신세포암종은 5년에 걸쳐 천천히 자랐다. CT에서 약한 조영증강을 보이는 소엽 모양 종괴였으며, MRI에서는 T1 강조영상에서 높은 신호 강도를, T2 강조영상에서 낮은 신호 강도를 보였다.

• 한국토양비료학회지
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• 제45권5호
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• pp.810-816
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• 2012

현행 살처분 가축사체의 처리방안 중 하나인 랜더링 처리방법을 사용하여 나온 부산물을 액비화 시키기 위해 산 및 알칼리 분해제를 사용하여 액화시켰으며, 분해제별 주입량 및 처리시간에 따른 랜더링 부산물의 분해율과 분해속도를 조사하였다. 산 및 알칼리 분해제별 주입량 및 처리시간에 따른 랜더링 부산물의 잔존량을 조사한 결과 분해제 중 가장 높은 잔존량을 나타낸 것은 $HNO_3$으로 다른 분해제는 10분이 경과한 후 랜더링 부산물의 잔존량이 대부분 50% 이하로 나타났다. 랜더링 부산물의 분해속도 K ($hr^{-1}$)는 KOH의 경우 랜더링 부산물 무게 대비 25% > 30% > 20% > 15% > 10%순으로 25%를 넣어줬을 때 가장 빨리 분해가 되었다. $H_2SO_4$의 경우 랜더링 부산물 무게 대비 30% > 25% > 20% > 15% > 10%순으로 넣었을 때 빨리 분해되었으며, NaOH도 이와 비슷한 경향이었다. 분해제별 랜더링 부산물의 분해율은 $HNO_3$의 경우에는 순도가 약 61% 정도로 다른 분해제들보다 낮아 분해율이 현저히 떨어졌으며, KOH, NaOH 및 $H_2SO_4$의 경우에는 랜더링 부산물을 25%와 30%를 넣었을 때 큰 차이가 없어 경제성 및 효율성을 고려하였을 때 분해제를 랜더링 부산물의 무게 대비 25%를 넣었을 경우가 가장 적합하다고 판단된다.