• 한국융합학회논문지
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• 제9권11호
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• pp.203-208
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• 2018

본 연구는 심혈관질환의 위험인자인 맥압과 혈청지질 그리고 폐기능 사이의 연관성이 존재하는지를 융합적으로 연구하고자 서울에 위치한 일개 회사의 사무직 종사자 109명(남성 51명, 여성 58명)을 대상으로 진행하였다. 대상자의 평균 연령은 $43.44{\pm}7.23$세, 남성 $43.44{\pm}7.27$세, 여성 $49.1{\pm}11.5$세였고, 키와 체중, BMI, 수축기혈압과 이완기 혈압을 측정하였고, 남녀의 차이가 있었다. 대상자의 맥압 평균은 $47.82{\pm}9.02mmHg$로 남녀의 차이는 없었다. %FVC의 평균은 $84.26{\pm}9.91%$로 남녀의 차이는 없었다. 맥압과 수축기혈압에서 남성(p<0.01), 여성(p<0.01)과 양의 상관관계를 나타내었으며, 남성에게서 FVC와 HDL는 양의 상관관계로 나타났다(p<0.05). 맥압과 %FVC는 음의 상관관계로 나타났다(-0.267, P<0.01). 회귀분석 결과 R2 은 0.071 로 맥압이 %FVC에 영향을 미치는 것으로 나타났다. 따라서, 혈압 측정에서의 맥압과 폐기능 검사는 심혈관계질환의 예측인자로 유용성이 있을 것으로 사료되며, 향후 심혈관질환의 연구의 융복합 연계가 필요할 것이다.

• 현장농수산연구지
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• 제22권2호
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• pp.135-146
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• 2020

딸기 재배지에서 점박이응애는 엽록소를 직접적으로 흡즙하여 생산량을 낮추는 주요 난방제 해충이다. 본 연구에서는 간이 약효 검정법을 개발하여 친환경유기농자재와 화학합성살비제가 포함된 10종의 약효를 평가하였다. 해당 기법은 4개의 약제를 3반복으로 실험할 수 있는 장점이 있어 시료 전처리 시간을 단축시킬 수 있다. 감수성 계통(SL_YS)을 포함한 전북 정읍(2020_JB_01), 충북 청주(2020_CB_01), 강원 고성(2020_GW_01)에서 채집한 지역 계통을 대상으로 약효를 평가한 결과 감수성 계통은 약 90-100% 사충률을 보인 반면에 지역 계통은 약제별로 상이하게 나타났다. 우수 약제를 선발하기 위해 약효 지수를 감수성 계통을 기준으로 산출하였다. 2020_JB_01 계통에 대한 우수 약제는 Test_02, Test_03 그리고 Test_10 약제가 선정되었다. 2020_CB_01 계통에 대해서는 Test_03과 Test_04가 선정되었다. 2020_GW_01 계통에서는 Test_01, Test_02, Test_03, Test_06 그리고 Test_10이 선정되었다. 계통 별 약제 반응이 다른 이유는 농가별 사용 약제와 살포 횟수가 다르기 때문인 것으로 사료된다. 약효 간이 검정법은 우수 약제 선발을 가능하게 하여 딸기 재배지 점박이응애 방제 효율을 증대시킬 것으로 기대된다.

• Journal of the Korean Data and Information Science Society
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• 제26권4호
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• pp.915-921
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• 2015

본 연구는 젖소 35,645두의 검정기록, 총 294,834를 이용하여 1회 착유량과 아세톤 및 ${\beta}$-히드록시부틸산 (BHBA) 함량의 (공)유전력을 추정하기 위하여 수행되었다. 아세톤과 BHBA의 평균 함량은 원유 1리터당 각각 $135.54{\pm}96.29{\mu}mol$과 $61.08{\pm}66.76{\mu}mol$였으며, 상위군과 하위군간 아세톤과 BHBA 함량의 차이는 각각 $6.12{\mu}mol/L$과 $4.35{\mu}mol/L$로서 모두 유의적인 차이(p<0.01)를 나타내었다. 1회 착유량의 유전력은 0.18~0.21, 아세톤 함량은 0.11~0.13과 BHBA 함량은 0.01~0.02의 범위에서 추정되었다. 아세톤과 BHBA의 표현형상관과 유전상관은 각각 0.44와 0.48로 추정되었으며, BHBA 함량이 $500{\mu}mol/L$ 이상부터는 아세톤과의 상관관계가 현저히 낮아지는 현상을 나타내었다. 하위군에서 1회 착유량에 대한 아세톤과 $Log_e$아세톤의 공유전력은 각각 0.02와 0.16으로 추정된 반면, BHBA와 $Log_eBHBA$의 공유전력은 각각 0.26과 0.32로 아세톤의 추정치보다 높게 나타났으며, 이러한 결과는 전체자료로 추정된 결과와도 일치하였다. 따라서 아세톤 함량보다는 1회 착유량과 공유전력이 높은 BHBA 함량이 1회 착유량에 대한 선발을 통하여 간접선발 반응을 유도하는데 더 효과적이며, 지시형질로서 바람직하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제26권7호
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• pp.961-970
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• 2013

Eight multiparous Holstein cows ($569{\pm}47$ kg of BW; $84{\pm}17$ DIM) were used to evaluate the effects of different levels of coarsely ground wheat (CGW) as replacements for ground corn (GC) in diets on feed intake and digestion, ruminal fermentation, lactation performance, and plasma metabolites profiles in dairy cows. The cows were settled in a replicated $4{\times}4$ Latin square design with 3-wk treatment periods; four cows in one of the replicates were fitted with rumen cannulas. The four diets contained 0, 9.6, 19.2, and 28.8% CGW and 27.9, 19.2, 9.6, and 0% GC on dry matter (DM) basis, respectively. Increasing dietary levels of CGW, daily DM intake tended to increase quadratically (p = 0.07); however, apparent digestibility of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were significantly decreased (p<0.01) in cows fed the 28.8% CGW diets. Ruminal pH remained in the normal physiological range for all dietary treatments at all times, except for the 28.8% CGW diets at 6 h after feeding; moreover, increasing dietary levels of CGW, the daily mean ruminal pH decreased linearly (p = 0.01). Increasing the dietary levels of CGW resulted in a linear increase in ruminal propionate (p<0.01) and ammonia nitrogen ($NH_3$-N) (p = 0.06) concentration, while ruminal acetate: propionate decreased linearly (p = 0.03) in cows fed the 28.8% CGW diets. Milk production was not affected by diets; however, percentage and yield of milk fat decreased linearly (p = 0.02) when the level of CGW was increased. With increasing levels of dietary CGW, concentrations of plasma beta-hydroxybutyric acid (BHBA) (p = 0.07) and cholesterol (p<0.01) decreased linearly, whereas plasma glucose (p = 0.08), insulin (p = 0.02) and urea nitrogen (p = 0.02) increased linearly at 6 h after the morning feeding. Our results indicate that CGW is a suitable substitute for GC in the diets of dairy cows and that it may be included up to a level of 19.2% of DM without adverse effects on feed intake and digestion, ruminal fermentation, lactation performance, and plasma metabolites if the cows are fed fiber-sufficient diets.

• Journal of Applied Biological Chemistry
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• 제63권2호
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• pp.153-159
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• 2020

축산물 중 살균제 prochloraz 및 그의 대사체의 잔류량을 함께 정량분석할 수 있는 단성분 분석법을 개발하고자 하였다. 축산물 중 prochloraz의 잔류분의 정의가 'prochloraz와 2,4,6-trichlorophenol기를 포함하고 있는 대사산물의 합을 prochloraz로 함'이기 때문에 prochloraz 및 대사산물을 모두 2,4,6-trichlorophenol (2,4,6-TCP)로 분해시킨 후 GC/ECD로 정량하는 정밀 분석법을 확립하였다. 대표 축산물인 소고기, 돼지고기, 닭고기, 우유 및 계란 중 prochloraz 잔류분을 acetone으로 추출하고 2,4,6-TCP로 분해한 후 dichloromethane으로 분배하고 aminopropyl SPE로 정제한 다음 2,4,6-TCP를 정량하였다. 본 분석법을 통한 기기정량한계와 분석법정량한계는 prochloraz의 경우 각각 0.01 ㎍/mL과 0.02 mg/kg이었으며 2,4,6-TCP는 0.005 ㎍/mL과 0.01 mg/kg이었다. 2,4,6-TCP를 이용한 표준검량선은 0.005-0.2 mg/L 범위에서 결정계수(R²) ≥0.995으로 직선성을 확인하였다. 분석법의 회수율은 분석정량한계 및 정량한계의 10배 처리수준에서 실시하여 모두 90% 이상이었고 변이계수는 10% 이하이었다. 따라서, 본 연구에서 확립된 분석법은 축산물 중 prochloraz 및 대사체를 정량분석하는데 우수한 정확성 및 정밀도를 보였으며, 축산물 중 prochloraz 잔류분석을 위한 정밀시험법으로써 분석개발기준을 충족하였다.

• 한국동물학회지
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• 제30권2호
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• pp.99-106
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• 1987

It is the purpose to investigate the physiological toxity and mutagenicity by heavy metal compounds such as potasium dichromate, sodium arsenite and selenium dioxide on the development of Drosophila melanogaster. These chemical compounds were highly toxic at every developmental stage of Drosophila. The relative values of fecundity, viability and developmental time obtained on food media containing 5 different levels of potasium dichromate (500, 600, 700, 800, and 900 ppm), also 5 different levels of sodium arsenite and selenium dioxide (10, 15, 20, 25 and 30 ppm). Resulting in the relative values of fecundity and viability generally decreased, on the contrary developmental time generally reduced as the concentration of chemicals in the food increased. A significant reduction in the fecundity was observed between 800 and 900 ppm (p<0. 01) on the potasium dichromate media, between 15 and 20 ppm (p<0. 05) on the sodium arsenite media, between 20 and 25 ppm (p<0. 02) on the selenium dioxide media. A significant reduction in the viability was observed between 700 and 800 ppm (p<0. 01) on the potasium dioxide media, between 25 and 30 ppm (p<0. 01) on the sodium arsenite media, between 20 and 25 ppm (p<0. 01) on the selenium dioxide media. The developmental time was significantly reduced between 800 and 900 ppm (p<0. 001) on the potasium dioxide media, between 15 and 20 ppm (p<0. 001) on the sodium arsenite media, and between 25 and 30 ppm (p<0. 01) on the selenium dioxide media.

• 한국동물학회지
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• 제30권2호
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• pp.107-116
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• 1987

It is the purpose to investigate the physiological toxity and mutagenicity by heavy metal compounds such as potasium dichromate, sodium arsenite and selenium dioxide on the development of Drosophila melanogaster. These chemical compounds were highly toxic at every developmental stage of Drosophila. The relative values of fecundity, viability and developmental time obtained on food media containing 5 different levels of potasium dichromate (500, 600, 700, 800, and 900 ppm), also 5 different levels of sodium arsenite and selenium dioxide (10, 15, 20, 25 and 30 ppm). Resulting in the relative values of fecundity and viability generally decreased, on the contrary developmental time generally reduced as the concentration of chemicals in the food increased. A significant reduction in the fecundity was observed between 800 and 900 ppm (p<0. 01) on the potasium dichromate media, between 15 and 20 ppm (p<0. 05) on the sodium arsenite media, between 20 and 25 ppm (p<0. 02) on the selenium dioxide media. A significant reduction in the viability was observed between 700 and 800 ppm (p<0. 01) on the potasium dioxide media, between 25 and 30 ppm (p<0. 01) on the sodium arsenite media, between 20 and 25 ppm (p<0. 01) on the selenium dioxide media. The developmental time was significantly reduced between 800 and 900 ppm (p<0. 001) on the potasium dioxide media, between 15 and 20 ppm (p<0. 001) on the sodium arsenite media, and between 25 and 30 ppm (p<0. 01) on the selenium dioxide media.

• 한국자원식물학회지
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• 제34권6호
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• pp.566-574
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• 2021

This research was carried out to investigate the correlation between soil chemical properties and bioactive compounds of Acer tegmentosum Maxim. The methods of determining bioactive compounds were determined by high performance liquid chromatography, that contained (-)-gallocatechin (0.04±0.01 ~ 0.43±0.28%), salidroside (0.90±0.06 ~ 3.86±0.59%), tyrosol (0.03±0.00 ~ 0.43±0.00%), (-)-catechin (0.05±0.01 ~ 0.37±0.14%), 6'-O-galloylsalidroside (0.02± 0.01 ~ 0.31±0.06%), (-)-epicatechin-gallate (0.01±0.00 ~ 0.04±0.01%). The soil chemical properties analysis such as soil pH, electric conductivity (EC), organic matter (OM), total nitrogen (TN), available phosphate (Avail. P₂O₅), exchangeable cation and cation exchange capacity (CEC) were performed following the standard manual. The correlation analysis between soil chemical properties and bioactive compounds of A. tegmentosum, soil pH, available phosphate and exchangeable cation (Ca²⁺ and Mg²⁺) were negatively correlated with content of salidroside. On the other hand, soil exchangeable cation (Na⁺) showed positive correlation with content of salidroside. The results of this study was able to investigate the correlation between soil chemical properties and bioactive compounds of A. tegmentosum.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.7007-7010
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• 2013

Background: In the literature, data concerning the relationship between breast cancer and HLA class II gene polymorphisms are limited, so the aim of this study was to determine if HLA-DQB1 and HLA-DRB1 MHC class-II alleles may confer susceptibility or resistance to the disease among Jordanian females. Materials and Methods: This case control study enrolled 56 Royal Hospital breast cancer patients and 60 age matched healthy controls, all of whom provided blood samples (2011-2013). A questionnaire was filled after signing a consent form and DNA was extracted, nucleic acids being amplified for assessment of HLA-DQB1 and HLA-DRB1 alleles by muliplex INNO-LiPA and allele typing carried out by reverse hybridization. Comparison of HLA-DQB1 and HLA-DRB1 allele distributions was carried out with paired t-test and chi-square statistics. Risk factors were assessed by odd ratios with 95% confidence intervals. Results: A significant negative correlation was observed between $HLADQB1^*$ 02 alleles and breast cancers (p=0.013). No significant associations were observed among $HLADQB1^*$ 03, 04, 05 and 06 or among $HLA-DRB1^*$ 01, 03, 04, 07, 08, 10, 11, 13, 14 and 15. Conclusions: $HLADQB1^*$ 02 alleles may provide positive protection against breast tumor risk among Jordanians, but not $HLADQB1^*$ 03, 04, 05 and 06 or $HLA-DRB1^*$ 01, 03, 04, 07, 08, 10, 11, 13, 14 and 15 alleles.

• 한국환경보건학회지
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• 제35권4호
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• pp.315-321
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• 2009

Under-fired charbroiling cooking processes are known as important contributors of particulate matter (PM). In this study, we characterized the emission of particulate matters from under-fired charbroiling cooking processes using the hood method. Accumulated mass concentration of $PM_{10}$ was 92.2~99.5% and particle size of 2.0~2.5 ${\mu}m$ was highest. The concentration of PM increased very sharply at the beginning of charbroiling meats and then gradually decreased as the charbroiling continued. PM concentration also increased very sharply when gravy from meat spilled onto the frame of fire. However, mass concentration during charbroiling using only charcoals was very low compared to that of meats. We estimated the emission factors of charcoal, pork belly and pork shoulder respectively; 0.01~0.02 g/kg, 5.02~6.26 g/kg, 2.86~4.15 g/kg of $PM_{2.5}$, 0.01~0.03 g/kg, 7.44~7.91 g/kg, 4.54~5.56 g/kg of $PM_{10}$, and 0.02~0.05 g/kg, 7.59~7.95 g/kg, 4.93~5.68 g/kg of TSP. The emission factors of charcoal were negligible and the emission factors of pork belly were higher than that of pork shoulder. Emission rates of particulate matters from under-fired charbroiling cooking process were estimated as 578,009~1,265,152 kg/yr of $PM_{2.5}$, 917,539~1,598,619 kg/yr of $PM_{10}$ and 996.358~1,606,703 kg/yr of TSP. But emission factors should be verified with an in-stack cascade impactor because the reported method involves some assumptions.