• Journal of Microbiology and Biotechnology
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• 제31권6호
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• pp.815-822
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• 2021

Indigenous fungus-feeding nematodes may adversely affect the growth and activity of introduced biocontrol fungi. Alginate pellets of the biocontrol fungus Trichoderma harzianum ThzID1-M3 and sclerotia of the fungal plant pathogen Sclerotinia sclerotiorum were added to nonsterile soil at a soil water potential of -50 or -1,000 kPa. The biomass of ThzID1-M3, nematode populations, and extent of colonization of sclerotia by ThzID1-M3 were monitored over time. The presence of ThzID1-M3 increased the nematode population under both moisture regimes (p < 0.05), and fungivores comprised 69-75% of the nematode population. By day 5, the biomass of ThzID1-M3b and its colonization of sclerotia increased and were strongly correlated (R² = 0.98), followed by a rapid reduction, under both regimes. At -50 kPa (the wetter of the two environments), fungal biomass and colonization by ThzID1-M3 were less, in the period from 5 to 20 days, while fungivores were more abundant. These results indicate that ThzID1-M3 stimulated the population growth of fungivorous nematodes, which in turn, reduced the biocontrol ability of the fungus to mycoparasitize sclerotia. However, colonization incidence reached 100% by day 5 and remained so for the experimental period under both regimes, although hyphal fragments disappeared by day 20. Our results suggest that indigenous fungivores are an important constraint for the biocontrol activity of introduced fungi, and sclerotia can provide spatial refuge for biocontrol fungi from the feeding activity of fungivorous nematodes.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.993-998
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• 2006

We earlier suggested a hierarchical regulatory network using defined modeling symbols and weights in order to improve the flux balance analysis (FBA) with regulatory events that were represented by if-then rules and Boolean logic. In the present study, the simulation results of the models, which were developed and improved from the previou model by incorporating a hierarchical regulatory network into the FBA, were compared with the experimental outcome of an aerobic batch growth of E. coli on glucose and tryptophan. From the experimental result, a diauxic growth curve was observed, reflecting growth resumption, when tryptophan was used as an alternativee after the supply of glucose was exhausted. The model parameters, the initial concentration of substrates (0.92 mM glucose and 1 mM tryptophan), cell density (0.0086 g biomass/1), the maximal uptake rates of substrates (5.4 mmol glucose/g DCW h and 1.32 mmol tryptophan/g DCW h), and lag time (0.32 h) were derived from the experimental data for more accurate prediction. The simulation results agreed with the experimental outcome of the temporal profiles of cell density and glucose, and tryptophan concentrations.

• 한국강구조학회 논문집
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• 제15권4호통권65호
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• pp.389-401
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• 2003

본 연구의 목적은 H형강 단면과 콘크리트의 합성단면으로 구성된 보와 기둥의 합성 뼈대구조물에 대해 신뢰성지수를 고려한 최적 단면을 설계할 수 있는 알고리즘을 개발하는데 있다. 합성 뼈대구조물의 최적화 문제는 단면 치수를 설계변수로 취하고 목적함수와 제약조건을 형성한다. 목적함수는 구조물의 총 경비로 형성하고, 제약조건식은 단면응력과 허용응력의 신뢰성지수를 고려하여 유도한다. 합성 뼈대구조물의 단문을 최적화하는 알고리즘은 수정 Newton-Raphson 탐사법을 사용하는 SUMT기법을 사용한다. 본 연구에서 개발된 최적화 알고리즘은 1층 1경간 합성 뼈대구조물과 5층 1경간 합성 뼈대구조물의 수치예에 신뢰성지수(${\beta}=3.0$, ${\beta}=0.0$)를 고려한 합성 뼈대구조물 설계의 실용화를 위하여 적용된다. 제안된 알고리즘의 최적화 가능성과 적용성 그리고 수렴성 등을 살펴보기 위하여 수치결과들을 비교 분석한다.

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.831-838
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• 2018

Trophic interactions of introduced biocontrol fungi with soil animals can be a key determinant in the fungal proliferation and activity. This study investigated the trophic interaction of an introduced biocontrol fungus with soil nematodes. The biocontrol fungus Trichoderma harzianum ThzID1-M3 and the fungivorous nematode Aphelenchoides sp. (10 per gram of soil) were added to nonsterile soil, and microbial populations were monitored for 40 days. Similar results were obtained when the experiment was duplicated. ThzID1-M3 stimulated the population growth of indigenous nematodes (p < 0.05), regardless of whether Aphelenchoides sp. was added. Without ThzID1-M3, indigenous nematodes did not increase in number and the added Aphelenchoides sp. nematodes almost disappeared by day 10. With ThzID1-M3, population growth of nematodes was rapid between 5 and 10 days after treatment. ThzID1-M3 biomass peaked on day 5, dropped at day 10, and then almost disappeared at day 20, which was not influenced by the addition of nematodes. In contrast, a large quantity of ThzID1-M3 hyphae were present in a heat-treated soil in which nematodes were eliminated. Total fungal biomass in all treatments peaked on day 5 and subsequently decreased. Addition of nematodes increased the total fungal biomass (p < 0.05), but ThzID1-M3 addition did not affect the fungal biomass. Hyphae of total fungi when homogenously distributed did not support the nematode population growth; however, hyphae of the introduced fungus did when densely localized. The results suggest that soil fungivorous nematodes are an important constraint on the hyphal proliferation of fungal agents introduced into natural soils.

• 전기전자학회논문지
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• 제23권1호
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• pp.14-21
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• 2019

본 논문에서는 MZR을 사용하여 2.4GHz WiFi대역에서 동작하는 온 보드(on-board) 초소형 안테나를 구현하였다. 설계한 안테나는 소형 단말기 PCB의 크기가 $78{\times}38{\times}0.8mm^3$이며, 시스템의 크기는 $63{\times}38{\times}0.8mm^3$이고, 방사부의 크기는 $15{\times}38{\times}0.8mm^3$인 제한조건에서 동작하는 초소형 안테나를 구현하였다. 급전구조는 시스템 보드의 좌측 상단에 급전 점을 설정하고, 안정적인 급전을 위해 CPW구조를 사용하였고, 급전부와 안테나의 결합은 자계결합구조를 사용하였다. MZR의 공진주파수는 직렬 커패시터와 셀의 인덕턴스에 의해서 결정됨으로 셀 사이의 갭, 셀의 길이, 인터디지털(interdigital) 커패시터의 길이, 방사부와 접지면의 간격에 대하여 분석하였으며, 그 결과를 사용하여 안테나를 설계 제작하였다. 제작한 안테나는 급전구조를 포함한 안테나의 전체크기는 $20.8{\times}9.0{\times}0.8mm^3$이며, 전기적인 길이는 $0.1664{\lambda}_0{\times}0.072{\lambda}_0{\times}0.0064{\lambda}_0$이다. 측정결과 10 dB 대역폭, 이득과 방향성은 각각 440 MHz(18.3%), 0.4405 dB, 2.722 dB이다. 방사패턴은 전 방향 특성을 가지고 있음을 확인하였으며, 초소형 단말기 안테나에 적용할 수 있음을 확인하였다.

• PNF and Movement
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• 제16권3호
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• pp.451-460
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• 2018

Purpose: The purpose of this study was to investigate the effects of a modified constraint induced movement therapy (mCIMT) using proprioceptive neuromuscular facilitation (PNF) on the upper extremity function and activities of daily living (ADLs) in patients with subacute stroke. Methods: Fourteen participants with subacute stroke were randomly assigned to a group using both mCIMT and PNF or a group using mCIMT alone. Each group underwent twenty sessions (1 h/d, 5 d/wk) for 4 weeks. Patients were assessed with the action research arm test (ARAT), the Fugl-Meyer assessment for the upper extremities (FMA-UE), the Modified Barthel Index (MBI), and motor activity logs (MALs; amount of use [AOU] and quality of movement [QOM]). Results: Both the experimental group and the control group showed significant intragroup improvement in the ARAT, FMA-UE, MBI, and MAL-AOU (p<0.05). The group using both mCIMT and PNF exhibited greater improvement in the ARAT, FMA-UE, MBI, and MAL-AOU than did the group using mCIMT alone. Statistical analyses showed significant differences in the ARAT (p=0.01), FMA-UE (p=0.01), MBI (p=0.00), and MAL-AOU (p=0.01) between the groups. Conclusion: This study applied mCIMT combined with PNF for subacute stroke patients, and the results showed significant improvements in the patients' upper extremity function and ADLs. Therefore, mCIMT using PNF may be more effective than mCIMT alone in improving upper limb function and ADLs in patients with subacute stroke.

• 한국통신학회논문지
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• 제24권12B호
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• pp.2369-2381
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• 1999

본 논문에서는 웨이브렛 변환을 이용하여 블록기반 변환 부호화 영상에서의 블록화 현상을 반복적으로 제거하는 방법을 제안하였다. 제안된 방법에서는 블록화 현상이 수직, 수평 방향의 블록 경계를 따라 수직, 수평으로만 나타나는 점에 착안하여, 블록화 현상이 있는 영상 신호를 수직, 수평 방향의 분리적인 1차원 신호의 집합으로 간주하고 Gaussian 형태 함수의 1차 도함수를 모 웨이브렛으로 하는 1차원 웨이브렛 영역에서의 평균 자승 오차를 최소화시키는 필터로써 첫 번째 스케일 웨이브렛 영역의 블록 경계 위치에서의 분산이 다른 위치에 비하여 유달리 크게 나타나도록 하는 블록화 현상에 의한 신호 성분을 제거하는 과정과 양자화에 관한 블록 집합으로 투영하는 과정을 반복적으로 수행하여 블록화 현상이 제거된 영상을 얻는다. 실험결과, 제안된 방법은 0.56 - 1.07dB의 PSNR 성능 향상뿐만 아니라 에지 몽롱화가 없이 블록화 현상이 거의 제거된 주관적 화질 개선을 보였다.

• 한국음향학회지
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• 제25권1호
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• pp.36-42
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• 2006

본 논문에서는 화자검증 시스템의 성능향상을 위해서 주성분 분석 (PCA) 기반 Mel-Frequency Discrete Wavelet Coefficients (MFDWC) 추출방법을 제안한다. 제안된 방법에서는 멜척도 (Mel-scale)를 근사화한 각 레벨 (level)의 각 노드 (node) 에너지를 계산하기 위해 기존의 평균치 대신 주성분 분석을 이용한 첫 번째 eigenvector를 이용한다. 이 eigenvecto.의 제곱의 합은 1로서 일반적인 가중 함수 (weighting function)의 조건을 만족하고, 또한 각 화자마다 서로 다른 값을 갖게 되므로, 화자의 특징을 더 잘 나타내는 MFDWC를 추출할 수 있다. 화자검증은 Gaussian Mixture Model (GMM) 기반의 백그라운드 모델과 화자 모델과의 점수를 비교하는 이진 결정 (binary decision) 방법을 이용하여 Universal 백그라운드 모델 (UBM)과 각 화자 모델의 값을 프레임단위로 비교하여 대상 화자의 수락/거부 여부를 결정하는 방법을 채택하였다. 특징 파라미터에 따른 화자 검증 성능변화를 확인하기 위하여 제안된 화자종속 가중함수를 이용한 MFDWC를 특징 파라미터로 이용한 경우와 Mel-Frequency Cepstral Coefficients (MFCC), Linear Predictive Cepstral Coefficients (LPCC), 기존의 MFDWC를 특징 파라미터로 이용한 경우에 대하여 성능비교실험을 수행한 결과 각각 $0.80\%,\;5.14\%,\; 6.69\%$의 향상된 성능을 나타내어 제안한 방법의 유효성을 확인할 수 있었다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 춘계학술대회 및 임시총회
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• pp.49-49
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• 2015

Over the past decade, clubroot has emerged as a major constraint to canola (Brassica napus) production in central Alberta, Canada. The number of fields with confirmed P. brassicae infestations in Alberta has increased steadily from 12 in 2003 to nearly 2,000 in 2014. Management of clubroot on canola has focused on sanitization of field equipment, soil amendments to reduce viable pathogen populations, long rotations out of susceptible crops and cropping of resistant cultivars. Clubroot resistance is the most effective and economical method of disease mitigation, but the recent identification of isolated P. brassicae populations with novel virulence phenotypes capable of overcoming resistance in most canola cultivars highlights the variable nature and adaptability of the pathogen. Recent studies have shown slight reductions in pathogen populations through crop rotations, but much more substantial reductions in spore populations in heavily infested areas near field entrances using fumigants such as Vapam (metam-sodium) or Basamid (dazomet). Greenhouse trials showed that seedling emergence, plant height and root weight increased, while primary and secondary infection and disease severity decreased with increased Basamid dosage. However, field trials showed some phytotoxicity. Application of Vapam at rates of 0.4 to $1.6mL\;L^{-1}$ soil resulted in 12-16 fold reductions in clubroot severity and primary and secondary infection. Vapam also was effective in reducing clubroot severity and improving canola seed yield under field conditions. These studies underscore the need for good resistance stewardship and for the integration of multiple products and practices for successful management of clubroot on canola.

• Earthquakes and Structures
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• 제11권1호
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• pp.141-163
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• 2016

Fiber models have been developed and applied to various structural elements such as shear walls, beams and columns. Only scarcely have fiber models been applied to circular foundation systems such as cast in drilled holes shafts (CIDH). In pile foundations with constraint head boundary conditions, shear deformations can easily contribute to the lateral pile response. However, soil structure interaction formulations such as the p-y method, commonly used for lateral pile design, do not include structural shear deformations in its traditional derivation method. A fiber model that couples shear and axial-bending behavior, originally developed for wall elements was modified and validated on circular cross sections (columns) before being applied to a 0.61 m diameter reinforced concrete (RC) pile with fixed head boundary conditions. The analytical response was compared to measured test results of a fixed head test pile to investigate the possible impact of pile shear deformations on the displacement, shear, and moment profiles of the pile. Results showed that shear displacements and forces are not negligible and suggest that nonlinear shear deformations for RC piles should be considered for fixed-head or similar conditions. Appropriate sensor layout is recommended to capture shear deformation when deriving p-y curves from field measurements.