• 제목/요약/키워드: -cyclodextrin

검색결과 573건 처리시간 0.029초

Acridine orange-β-cyclodextrin 내포 착물을 이용한 카페인의 분광형광법 정량 (Spectrofluorometric determination of caffeine using acridine orange-β-cyclodextrin inclusion complex)

  • 박종휘;최희선
    • 분석과학
    • /
    • 제26권6호
    • /
    • pp.353-356
    • /
    • 2013
  • A method on the determination of caffeine in beverage with acridiene orange-${\beta}$-cyclodextrin (CD) inclusion complex was developed. The conditions such as pH of the sample solution and concentration of acridine orange and ${\beta}$-CD were optimized to 12.0(${\pm}0.5$), $1.9{\times}10^{-6}M$ and $1.25{\times}10^{-3}M$, respectively. Under these optimum conditions, the calibration curve of caffeine was obtained over concentration range of $5{\times}10^{-5}{\sim}1.1{\times}10^{-3}M$. The detection limit was $1.0{\times}10^{-5}M$. The relative errors(%) in beverage samples were less than 5.0%.

호알칼리성 Bacillus sp. C-21에 의한 Cyclodextrin Glucanotransferase의 생산 (Production of Cyclodextrin Glucanotransferase from Alkalophilic Bacillus sp. C-21)

  • 강희정;채기수;선우양일
    • 한국식품영양학회지
    • /
    • 제8권3호
    • /
    • pp.253-261
    • /
    • 1995
  • A strain of alkalophilic Bacillus sp. C-21 has been Isolated from sold. The strain was capable of producing large amount of cyclodextrin glycosyltransferase (CGTase) in the high alkaline pH medium. The preferable medium composition was determined to be as follows : 1.0% soluble starch, 1.0% peptone, 0.5% yeast extract, 0.1% K2HP04, 0.02% MgSO4.7H2O and 1.0% Na2CO3(pH 10.0) The highest enzyme production was observed after 30hours of cultivation at 33$^{\circ}C$. The optimum temperature and pH for the activity of crude enzyme were 6$0^{\circ}C$ and 6.0, respectively. The enzyme was stable between pH 6.0 and 9.6, and up to 55$^{\circ}C$.

  • PDF

저콜레스테롤 난황 제조시 생성되는 부산물로부터 콜레스테롤의 분리 정제 (Separation and Purification of Cholesterol from By-product of Low Cholesterol Egg Yolk)

  • 유익종;조혜연;박우문;전기홍;최성유
    • 한국축산식품학회지
    • /
    • 제20권1호
    • /
    • pp.36-43
    • /
    • 2000
  • $\beta$-cyclodextrin adsorption and saponification methods were applied to isolate and purify cholesterol from the by-product of the low-cholesterol egg yolk product. They by-product was prepared from processing low-cholesterol egg yolk followed by extracting with chloroform to remove $\beta$-cyclodextrin and concentrated to 3,069 mg% cholesterol. When $\beta$-cyclodextrin method between two purification methods was applied, 50% ethanol as a solvent showed higher cholesterol concentration of 5.82% rather than the other solvents. Repeated purification of 3 times could not improve the cholesterol concentration significantly(p<0.05). In case of purification using saponification method, hexane as a solvent for extraction of unsaponificated materials was more efficient to increase cholesterol concentration than chloroform and ether. 60 times(v/w) saponification solution (95% ethanol:33% KOH = 94:6) of sample weight was most effective to increase the cholesterol concentration of 35.7%. Repeated purification process by saponification method could increase cholesterol concentration to 95.7% by 4 times repetition.

  • PDF

Flurbiprofen과 Cyclodextrin과의 포접화물(包接化物)에 관한 연구(硏究) (Inclusion Compound of Flurbiprofen with Cyclodextrin)

  • 백완숙;용재익;김길수
    • Journal of Pharmaceutical Investigation
    • /
    • 제15권1호
    • /
    • pp.15-21
    • /
    • 1985
  • The inclusion of ${\alpha}-cyclodextrin$ and ${\beta}-cyclodextrin$ with flurbiprofen in aqueous phase was investigated by UV absorption and circular dichroism spectroscopies. The inclusion complex in solid powder form were made by the freeze-drying and coprecipitation methods in molar ratio 1:1. The inclusion complex formation was confirmed by infrared absorption spectroscopy. The freeze-drying method was successful in obtaining the inclusion compounds compared with the coprecipitation method. The dissolution of solid flurbiprofen inclusion complex was examined in comparison with those of flurbiprofen alone. The inclusion complex obtained by freeze-drying method increased the dissolution rate.

  • PDF

Inclusion Complexation of Cyclodextrin with Prothionamide in Aqueous Solution

  • Kim, Shin-Tae;Kim, Shin-Keun
    • Journal of Pharmaceutical Investigation
    • /
    • 제12권4호
    • /
    • pp.132-144
    • /
    • 1982
  • The inclusion of ${\beta}-cyclodextrin$ $({\beta}-CyD)$ with prothionamide in aqueous phase was investigated by circular dichroism(CD), ultraviolet (UV) absorption, and solubility technique. The results suggested that a region of drug chromophore was located within the asymmetric center of ${\beta}-cyclodextrin$. Solubility and spectral changes were quantitatively treated to obtain stoichiometric ratio, which was found to be 1 : 1, and formation constants which were determined by solubility, CD, and UV method were 257, 367, and 389 $M^{-1}$, respectively. Also, the formation constant of the inclusion complex was determined by CD method at various pH. The result was that $K_c$ depended upon the pH of medium, and this fact also supported that thioamide moiety was accomodated in the cavity of ${\beta}-cyclodextrin$.

  • PDF

클로로프로파미드와 싸이클로덱스트린과의 포접 화합물에 관한 연구 (Inclusion Complexation of Chlorpropamide with Cyclodextrins)

  • 정경혜;김길수;구영순
    • 약학회지
    • /
    • 제30권2호
    • /
    • pp.87-95
    • /
    • 1986
  • Inclusion complexation of $\alpha$-cyclodextrin($\alpha$-CD), $\beta$-cyclodextrin($\beta$-CD) and tri-O-methyl-$\beta$-cyclodextrin(tri-O-methyl-$\beta$-CD) with chlorpamide(CPA) in aqueous solution and in the solid state were studied by the solubility method, spectroscopy (UV, IR), differential scanning calorimetry (DSC) and powder X-ray diffractometry, and all their molar ratios were found to be 1:1. The solid complexes of CPA with three kinds of cyclodextrins were prepared by a freezedrying method, and their dissolution behaviors were examined. As a result, the release of CPA from the inclusion complexes was significantly improved. The intrinsic dissolution rate of CPA in cyclodextrin inclusion complexes was about 51 times ($\alpha$-CD inclusion complex) and 12 tmies ($\beta$-CD inclusion complex) larger than that of intact CPA.

  • PDF

수성2상계를 이용한 Cyclodextrin Glucanotransferase 분리 및 회수 (Separation and Recovery of Cyclodextrin Glucanotransferase Using Aqueous Two-Phase Systems)

  • 김진현;홍승서;이현수
    • KSBB Journal
    • /
    • 제15권6호
    • /
    • pp.556-559
    • /
    • 2000
  • Cyclodextrin Glucanotransferase(EC 2.4.1.19 : 1,4-${\alpha}$-glucano) transferase, cyclizing; CGTase) can be separated and recovered in an aqueous two-phase system composed of poly(ethylene glycol)(PEG)/dextran and PEG/salt. In an aqueous two-phase system consisting of PEG 35000 (5%) and dextran T2000 (7%), all cell and debris were collected at the interphase. CGTase partitioned to the denser dextran phase at an yield of 83.4%. On the other hand, in an aqueous two-phase system consisting of PEG 35000 (10%) and sodium phosphate (15%), CGTase partitioned to the denser salt phase at an yield of 95.5%. In order to recover CGTase using an aqueous two-phase system, the PEG/salt system proved to be more efficient than the PEG/dextran system in terms of yield and cost.

  • PDF

약물의 약제학적 연구(제 3보) -${\beta}-$시클로덱스트린과의 복합체 형성에 의한 설파메톡사졸의 용출 및 안정성 향상- (Phamaceutical Studies on the Drug (III) -Enhancement of Dissolution and Stability of Sulfamethoxazole by ${\beta}-Cyclodextrin$ Complexation-)

  • 이현종;김수억;서성훈
    • Journal of Pharmaceutical Investigation
    • /
    • 제18권4호
    • /
    • pp.181-186
    • /
    • 1988
  • Inclusion complex of sulfamethoxazole with ${\beta}-cyclodextrin$ was prepared by freeze-drying method in molar ratios of 1:1, 1:1.25, 1:1.5 and 1:1.75, and the complex formation was identified by ultraviolet and infrared spectroscopies, powder X-ray diffractometry and differential scanning calorimetry. Dissolution rate and solid state stability of the complex were investigated in comparison with those of sulfamethoxazole powder and the physical mixture of sulfamethoxazole with ${\beta}-cyclodextrin$. As a result, the dissolution rate and the stability of solid complexes in various relative humidity conditions increased more remarkably than those of sulfamethoxazole powder and physical mixture. But the difference according to molar ratio of the complex was not recognized.

  • PDF

막 생물반응기를 이용한 Cyclodextrin 동족체의 효소적 생산 (Enzymatic Production of Cyclodextrin Homologues Using Membrane Bioreactors)

  • 홍준기;염경호
    • 한국막학회:학술대회논문집
    • /
    • 한국막학회 1998년도 추계 총회 및 학술발표회
    • /
    • pp.82-85
    • /
    • 1998
  • 1. 서론 : Cyclodextrin(CD) 동족체(homologues)는 $\alpha$-, $\beta$-, $\gamma$-CD로 구분되며, 이들 각각은 $\alpha$-D-glucopyranose 단위체 6,7, 및 8개가 비환원성 환상구조로 연결된 cyclic maltooligosaccaride의 일종으로 외부는 친수성이고, 내부는 소수성인 공동 구조를 갖고 있다. 따라서 각 CD는 동공의 크기가 달라 다른 크기의 소수성 물질들과 선택적인 포접화합물 (inclusion compound)을 형성하는 특징이 있다. CD 동족체는 전분 분해 효소인 cyclodextrin glycosyltransferase(CGTase)에 의해 전분으로부터 생산되는데, 반응용액 내에서의 CD 동족체 농도가 어느 한계값 이상으로 높아지면 생산물 저해와 다른 환원당으로의 분해 때문에 생산성이 감소하여 이의 효과적 생산에 어려움이 있다. 본 연구는 dead-end 및 cross-flow형 막 생물반응기를 사용하여 CGTase에 의한 전분의 CD 동족체로의 분해반응시 생산물 저해를 억제시켜 생산성을 향상시키고, 동시에 조작조건 변화에 따른 생산물인 CD 동족체의 효과적인 연속분리 가능성을 검토하였다.

  • PDF

콜레스테롤을 제거한 치즈의 개발에 관한 연구 (A Development of Cholesterol Removed Cheese)

  • 정청송
    • 한국관광식음료학회:학술대회논문집
    • /
    • 한국관광식음료학회 2002년도 학술논문발표회
    • /
    • pp.83-98
    • /
    • 2002
  • 본 실험은 $\beta$-cyclodextrin 을 사용하여 cholesterol을 제거한 균질우유로부터 cholesterol이 제거된 치즈 제조의 조건을 규명하고, 그 조건에 따라 제조된 cheese 의 특성 및 관능 검사를 함으로써 cholesterol이 제거된 cheese의 개발 가능성을 조사하는데 그 목적을 두고 있다. 치즈 제조에 적합하다고 사료되는 낮은 균질압력에서 효과적인 cholesterol 제거율을 알아보기 위해 균질압력 0, 250, 500, 750, 1000psi(0, 17.5, 35, 52.5, 70kg/$\textrm{cm}^2$)와 균질온도 40, 50, 60, 7$0^{\circ}C$ 그리고 $\beta$-cyclodextrin 첨가량 1.0, 1.5 2.0% 등의 3가지 조건에 따라 우유를 처리하여 cholesterol이 제거된 cheese의 제조에 적합한 조건으로는 균질압력 1 000psi(70kg/$\textrm{cm}^2$), 균질온도 7$0^{\circ}C$, $\beta$-cyclodextrin 첨가량 2.0% 관찰되었다. 따라서 균질온도는 7$0^{\circ}C$로 정하고, 균질압력을 750, 1000, 13009si(52.5, 70, 91kg/$\textrm{cm}^2$)로, $\beta$-cyclodextrin 첨가량을 1과 2%로 처리하여 제조한 6가지의 cheese와 균질을 하지 않고 $\beta$-cyclodextrin의 처리를 하지 알은 control 치즈간의 수율, cholesterol 제거율, mettability, stretchability, oiling off, 조직검사, 관능검사 등의 실험을 실시하여 control 치즈와 가장 유사한 cholesterol이 제거된 cheese 개발의 가능성을 시도하였고, 그 결과는 다음과 같다. 1. Cholesterol 을 제거한 cheese의 제조에서 최적조건은 균질압력 1000psi(70kg/$\textrm{cm}^2$), 균질온도 7$0^{\circ}C$, $\beta$-cyclodextrin 첨가량 2%였으며, 이때 우유의 cholesterol의 제거율이 86.05%로 가장 높게 나타났다. 2. Cholesterol을 제거한 cheese들의 수율은 모두 12.53%(control 10.54%) 이상으로 균질처리가 cheese의 수율을 18.88% 이상 향상시키는 것으로 나타났다. 3. 유지방 함량 23.80% 인 control 치즈의 cholesterol 함량은 81.47mg/l00g 이었고, 균질압력 1300psi(91kg/$\textrm{cm}^2$)에 $\beta$-cyclodextrin 2%를 첨가한 cheese 에서는 cholesterol 함량이 20.15 mg/100g으로 cholesterol 제거율이 75.27%로 가장 높게 나타났다. 4. Meltability는 균질압력 1300psi(91kg/$\textrm{cm}^2$)에 $\beta$-cyclodextrin 1과 2%로 처리한 치즈에서 2.25cm(control 3.34cm)로 가장 낮았으며, 균질압력이 증가할수록 meltability가 감소하여 치즈의 품질을 저하시켰다. 5. Control 치즈의 stretchability 는 30cm 이상 늘어나 가장 양호한 수치인 5점을 나타낸 반면, cholesterol을 제거한 cheese 에서는 5~10cm 사이를 나타내어 2점으로 stretchability가 저하된 것을 볼 수 있었다. 6. Oiling off는 균질압력 1300psi(91kg/$\textrm{cm}^2$)에 $\beta$-cyclodextrin 1과 2% 처리한 치즈에서 0.03%(control 2.46%)로 가장 낮았으며, 균질압력이 증가할수록 oiling off는 감소하여 치즈의 외관상 품질을 향상시켰다. 7. Hardness, gumminess, chewiness는 균질압력 1300psi(91kg/$\textrm{cm}^2$)에 $\beta$-cyclodextrin 1%로 처리한 치즈에서 각각 가장 낮은 2.03kg/$\textrm{cm}^2$, 2.l8kg, 2.24kg/cm(control 5.46kg/$\textrm{cm}^2$, 5.24kg, 4.87kg/cm)를 나타내었으며, control과 cholesterol을 제거한 치즈들 사이에 유의적 (p<0.05) 차이를 보이며 큰 감소를 나타내었다. 8. Cholesterol을 제거한 cheese 의 appearance와 flavor는 1300psi(91kg/$\textrm{cm}^2$)일 때 각각 가장 높은 5.56과 4.63(control 4.00)으로 균질압력이 증가하면서 향상되었으며, texture score는 균질압력 1300psi(91kg/$\textrm{cm}^2$)에 $\beta$-cyclodextrin 2%일때 1.22(control 4.00)로 가장 낮은 수치를 나타내었고, 균질이 cheese의 texture score를 상당히 저하시키는 것을 알 수 있었다. 9. 이 실험결과, 균질압력 1300psi(91kg/$\textrm{cm}^2$)에 $\beta$-cyclodextrin 2% 첨가한 cheese에서 cholesterol 제거율이 75.27%로 가장 높았으며, 균질처리가 cheese의 수율, oiling off, appearance score, flavor scope등을 향상시켰고, meltability, stretchability, hardness, gumminess, chewiness, texture score를 저하시키는 것으로 나타나 cholesterol이 상당부분 제거된 cheese의 개발 가능성이 관찰되었다.

  • PDF