• 한국식품영양과학회지
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• 제20권2호
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• pp.156-161
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• 1991

고추가루의 돌연변이유발, 보돌연변이 및 항돌연변이 효과를 검토하기 위해 매운맛의 재래종과 덜 매운맛의 교배종을 극성이 다른 용매로 3분획한 것과 고추의 주요한 매운 성분으로 알려진 capsaicin을 Ames mutagenicity test를 이용하여 연구하였다. 고추가루 추출획분들을 모두 direct한 돌연변이유발성이 없는 것으로 나타났고 MNNG가 존재할 때의 보돌연변이 효과도 나타나지 않았다. 또한 이들은 rat S9이 존재할 때의 indirect한 mutagenicity도 일으키지 않는 결과를 보였다. 고추가루의 주요 매운 성분인 capsaicin도 Aroclor 1254, phenobarbital로 induce시킨 rat S9과 Aroclor 1254로 induce시킨 hamster S9 등의 activation system을 이용하여도 돌연변이유발의 효과는 나타나지 않았다. 그러나 고추가루 추출획분들은 TA100 strain에서 모두 aflatoxin $B_1$의 mutagencity를 오히려 억제시키는 결과를 나타내었는데 이 효과는 고추가루의 첫번째 획분(1st fraction)이 가장 컸었다.

• International Journal of Concrete Structures and Materials
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• 제9권1호
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• pp.1-20
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• 2015

This work is devoted to the study of fresh and hardened properties of concrete containing recycled gravel. Four formulations were studied, the concrete of reference and three concretes containing recycled gravel with 30, 65 and 100 % replacement ratios. All materials were formulated on the basis of S4 class of flowability and a target C35 class of compressive strength according to the standard EN 206-1. The paper first presents the mix design method which was based on the optimization of cementitious paste and granular skeleton, then discusses experimental results. The results show that the elastic modulus and the tensile strength decrease while the peak strain in compression increases. Correlation with the water porosity is also established. The validity of analytical expressions proposed by Eurocode 2 is also discussed. The obtained results, together with results from the literature, show that these relationships do not predict adequately the mechanical properties as well as the stress-strain curve of tested materials. New expressions were established to predict the elastic modulus and the peak strain from the compressive strength of natural concrete. It was found that the proposed relationship E-$f_c$ is applicable for any type of concrete while the effect of substitution has to be introduced into the stress-strain (${\varepsilon}_{c1}-f_c$) relationship for recycled aggregate concrete. For the full stress-strain curve, the model of Carreira and Chu seems more adequate.

• 벤처창업연구
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• 제9권6호
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• pp.153-161
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• 2014

본 연구는 Kano 모델을 이용하여 화장품 구매에 영향을 미치는 마케팅 믹스 요인을 분석하기 위해, 선행적 연구를 바탕으로 마케팅 믹스 요인 분류에 따라 긍정적 질문과 부정적 질문을 동시에 설문하여 매력적 품질, 일원적 품질, 당연적 품질을 규명하고자 하였다. Timko's Analysis 고객 만족과 불만족 계수를 이용한 화장품 구매에 영향을 미치는 마케팅 믹스 요인을 분석한 결과 촉진전략 중 가격할인 정책과 가족/ 주변인의 추천 전략이 매력적 품질로 나타났다. 제품 전략의 효능과 효과, 사용감, 피부적합성은 일원적 품질로 나와 고객의 요구를 충족시키면 선형적으로 그 만족도가 증가하는 것으로 파악되었다. 반면에 제품 측면의 용기, 포장 디자인, 색상, 브랜드 인지도와, 가격 측면의 낮은 가격, 중간가격 수준과 유통 측면의 구매경로, 촉진 측면의 매체 광고 에 대해서는 무관심 품질로 나타나서 이들 요인에 대하여는 과도한 예산을 집행하는 것은 투입대비 효과가 높지 않을 것으로 판단해 볼 수 있다.

• 한국식품조리과학회지
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• 제15권6호
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• pp.611-617
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• 1999

자동산화된 유지의 산패도와 돌연변이원성과의 관련성을 알아보고자 대우유를 $60{\pm}2^{\circ}C$에서 자동산화시키면서 과산화물가가 일정한 수치에 도달한 시료를 대상으로 Salmonella typhimurium 변이균주 중 구조이동형(frame shift) 변이균주인 TA98, TA1537과 염기치환형(base-pair substituent) 변이균주인 TA100, TA1535 및 102를 사용하여 Ames test를 수행하였다. TA98은 대사활성화법에서, TA100은 직접법에서, TA1535 및 TA1537은 직접법과 대사활성화법 모두에서 돌연변이 활성이 인정되었으나 TA102는 시료유지의 전자동산화기간에서 돌연변이 활성이 나타나지 않았다. 또한 돌연변이원성이 과산화물가가 높은 시기에 나타나 유지의 과산화물 함량과 돌연변이 활성간에 갚은 관련성이 있는 것으로 생각되었다.

• 대한수의학회지
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• 제38권4호
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• pp.793-802
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• 1998

Salmonellosis caused by a number of serotypes of Salmonella is an infectious, acute or chronic, zoonotic disease and characterized by enteritis and diarrhea, septicemia in animal. In these studies we investigated the prevalent serotypes of Salmonella causing animal salmonellosis in Korea and the 71 strains of Salmonella spp. were isolated from materials such as mesenteric lymph nodes, fecal samples from slaughtered animal. With the identification test results, the most prevalent serotypes were, in order, S stanley 31 strains (43.7%), S typhimurium 19 strains (26.8%) and S montevideo 11 strains (15.5%), respectively. And we could establish the method for detection of antibodies to broad variety of Salmonella serotypes. Lipopolysaccharide(LPS) antigen extracted from Salmonella was more sensitive and specific than outer membrane protein antigen from that for detection of Salmonella antibody by using an indirect ELISA. The optimal concentration of antigen was 100ng/ml of LPS, the dilutions of conjugate and serum were 1 : 1,000~2,000 and 1 : 200~400, respectively. The mix LPS-ELISA which was used by mixing LPS from S typhimurium (group B), S choleraesuis (group C) and S enteritidis (group D) were more rapid and effective than that used LPS from individual strain for detection of Salmonella serogroup O4, O7 and O9 antibody at the same time. We could obtain the high values of optical density ($0.73{\pm}0.32$) by mix LPS-ELISA on the farm which had occurred salmonellosis, but very low values of $0.17{\pm}0.06$ on the negative farm of salmonellosis. So, the mix LPS-ELISA may be used to monitor the serological surveillance for the presence of infection with a number of serotypes of Salmonella and would be useful for prevention and control of salmonellosis in animal.

• 한국의상디자인학회지
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• 제9권2호
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• pp.155-166
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• 2007

The purpose of the research is to provide the operation plans of internet marketing for effective application as marketing tools by analyzing the application status of marketing mix through the 30 websites of sportswear brands. Firstly, a study on the marketing mix strategy of sportswear brands presents that most brands put a high priority on promotion strategy by achieving online marketing. Secondly, compared with the other company brands which have online shopping mall, the website of sportswear brands consists of centered brand image. Therefore, based on the analysis about the variety of products and merchandise picture in each item and detailed information offer of neo-products introduction, marketing mix is insufficient in the website of sportswear and the price strategy has the least information. After website of sportswear brands is connected with online-shopping mall, guidance for its price and product is performed mainly. Consequently the sales promotion strategy of price needs development through the incentive and discount price. Promotion strategy occupies more parts than any other strategy. Especially, it allows a high proportion of the variety of events and information for the customer services. Also it needs to improve its bulletin board and Q&A board on the website for better communication with customers. Furthermore distribution strategy focuses on information such as location, contact numbers and address of the online shopping mall. Although online shopping mall has its own advantage which is marketing on the internet, it is not yet in progress.

• 한국환경성돌연변이발암원학회지
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• 제18권2호
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• pp.77-82
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• 1998

In order to compare the suppressive effect of galangin on the genotoxicity by N-methyl-N-nitrosourea (MNU) or benzo[a]pyrene B(a)P, in vivo micronycleus test using mouse peripheral blood and in vitro sister chromatid exchange(SCE) test using mouse spleen lymphocytes were performed. MNU or B(a)P-induced micronucleated reticulocytes in vivo was decreased by the simultaneous treatment of galangin. MNU or B(a)P-induced SCEs in vitro was also decreased by the simultaneous treatment of galangin. On the other hand, the determinations of [$^3$H]MNU-induced total DNA binding and methylated DNA were performed to find out the mechanism of action. [$^3$H]MNU-induced total DNA binding was inhibited by the treatment of galangin in calf thymus DNA. HPLC analysis of DNA hydrolysates showed that galangin caused a decrease of 7-methyl guanine and $O^{6}$-methyl guanine in calf thymus DNA. To elucidate the action mechanism of galangin against B(a)P, alteration of B(a)P metabolism was studied. Galangin inhibited B(a)P metabolism in the presence of S-9 mix and decreased B(a)P-DNA binding in calf thymus DNA with S-9 mix.

• 한국환경성돌연변이발암원학회지
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• 제17권1호
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• pp.17-22
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• 1997

We observed the frequency of chromosome aberrations induced by UVB irradiations, and the suppressing effect of tannic acid on chromosome aberrations induced by UVB irradiations in CHL cells, which is a phenolic compound, a hydrolysate of tannin and a components of green tea. UVB doses used for the frequency of chromosome aberrations were from 0.2 to 1.6 KJ/m$^2$ and tannic acid concentrations were from 1.16 $\mu$g/ml to 37.50 $\mu$g/ml. For the observation of suppressing effect of tannic acid on UVB-induced chromosome aberrations, UVB dose was 1.6 KJ/m$^2$ and tannic acid concentrations were 1.0, 2.0, 4.0 $\mu$g/ml. In our study, tannic acid was treated for 24 hours in CHL, cells after UVB irradiation without S9 mix or for 6 hours with S9 mix. From this study, we obtained the following results : (1) The frequency of chromosome aberrations UVB induced were dose-dependently increased. (2) The tannic acid did not induce chromosome aberrations in cultured Chinese hamster cells. (3) UVB-induced chromosome aberrations were suppressed by tannic acid at every concentration from 1.0 $\mu$g/ml to 4.0 $\mu$g/ml with or without metabolic activation. These results suggest that the tannic acid acts as an inhibitor to UVB-induced clastogenicity of the cultured cell.

• 한국식품위생안전성학회지
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• 제11권1호
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• pp.41-50
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• 1996

The yields of solvent fraction of irradiated red ginseng powder were increased in the order of petroleum ether(PE)

• Biomolecules & Therapeutics
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• 제4권3호
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• pp.224-231
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• 1996

DA-5018, a non-narcotic analgesic agent, was examined for mutagenicity in the reverse mutation test on bacteria, chromosomal aberration test on cultured mammalian cells and micronucleus test on mice. The reverse mutation test was performed by a plate incorporation method with or without a metabolic activation system(S9 mix) using Salmonella typhimurium strain TA100, TA1535, TA98 and TA1537. DA-5018 did not significantly increase revertant colonies in any of the test strains under any conditions at concentrations ranging from 0.0049 to 1.25 mg/plate, compared with the vehicle control. In the chromosomal aberration test using cultured Chinese Hamster Lung(CHL) cells, DA-5018 did not increase the number of aberrant cells in the presence or absence of S9 mix at concentrations of 0.016 mM/plate to 0.25 mM/plate, compared with the vehicle control. In the micronucleus test, male ICR mice were given DA-5018 intraperitoneally at a dose level of 0.55, 1.10 and 2.20 mg/kg. The incidence of bone marrow micronucleated polychromatic erythrocytes in the DA-5018 treated mice was not significantly different from that of the vehicle control. These results indicate that DA-5018 does not have mutagenic potential under the present test conditions.