• Title/Summary/Keyword: -9

Search Result 174,504, Processing Time 0.098 seconds

Cloning and expression of cDNA for chemokine receptor 9 from Olive flounder, Paralichthys olivaceus

  • Kim, Mu-Chan;An, Geun-Hee;Park, Chan-Il
    • 한국어병학회지
    • /
    • 제20권3호
    • /
    • pp.299-306
    • /
    • 2007
  • Cysteine-cysteine chemokine receptor 9 (CCR9) homologue cDNA was isolated from olive flounder leukocyte cDNA library. Olive flounder CCR9 homologue consisted of 1709 bp encoding 367amino acid residues. When compared with other known CCR peptide sequences, the most conserved region of the olive flounder CCR9 peptide is the seven transmembranes. A phylogenetic analysis based on the deduced amino acid sequence showed the homologous relationship between the olive flounder CCR9 sequence and that of Mouse CCR9. The olive flounder CCR9 gene was predominantly expressed in the Peripheral blood leukocytes (PBLs), kidney, spleen, and gills.

Effect of Cell Source and pH of Culture Medium on the Production of Canthin-6-one Alkaloids from the Cell Cultures of Tongkat Ali (Eurycoma longifolia Jack)

  • Mahmud, Luthfi-Aziz;Chan;Boey
    • Journal of Plant Biotechnology
    • /
    • 제6권2호
    • /
    • pp.125-130
    • /
    • 2004
  • Callus and cell suspension cultures of Eurycoma longifolia Jack could be an alternative supply of 9-hydroxycanthin-6-one and 9-methoxycanthin-6-one. The callus tissues were initiated from leaves of different trees. The friable calli were used for the preparation of the cell suspension cultures of E. longifolia. The leaf explant of tree Eu-9 produced the most callus and also induced high cell biomass in the cell suspension culture, but it produced low quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. The leaf explant from tree Eu-8 produced low quantity of callus and cell biomass, but produced the highest quantity of 9-methoxycanthin- 6-one and 9-hydroxycanthin-6-one. Optimum production of cell biomass was obtained on cell culture medium with pH 5.75 prior to autoclaving, but high alkaloids content could be induced in culture medium in acidic condition with pH 4.75 and 5.25 prior to autoclaving.

한우에서 경정맥, 경동맥 및 갑상선의 초음파상 (Ultrasonography Of Jugular Vein, Carotid Artery and Thyroid Glands in Native Korean Cattle)

  • 김명철;변홍섭
    • 한국임상수의학회지
    • /
    • 제17권1호
    • /
    • pp.205-208
    • /
    • 2000
  • 소에서 경정맥, 경동맥 및 갑상선의 초으파상에 관한 기초자료를 확립하기 위하여 본 연구를 실시하였다. 한우 9두에서 6개월경으로부터 12개월령에 도달할 때까지 1개월 간격으로 성장에 따른 경정맥, 경동맥 및 갑상선의 초음파상을 측정하였다. 경정맥, 경도맥 및 갑상선은 경부의 양측에서 관찰하였다. 경정맥의 내경은 6, 7, 8, 9, 10, 11, 12 및 13개월령에서, 각각 8.5, 8.7, 8.8, 9.5, 10.0, 10.5, 11.5 및 12.2mm를 나타내었다. 경동맥의 내경은, 6, 7, 8, 9, 10, 11, 12 및 13개월령에서, 각각 7.4, 7.5, 8.6, 9.1, 9.4, 9.7, 10.1 및 10.3mm 이었다. 갑상선엽의 길이는 6, 7, 8, 9, 10, 11, 12 및 13개월령에서, 각각 28.0, 32.1, 36.4, 42.2, 51.7, 53.2, 55.1 and 58.8mm이었다. 갑상선엽의 높이는 6, 7, 8, 9. 10, 11, 12 및 13개월령에서, 각각 13.6, 15.3, 16.1, 20.7, 22.9, 25.6, 28.9 and 29.4mm를 나타내었다. 본 연구에서는 초음파측정 결과는 한우에서의 경정맥, 경동맥 및 갑상선에서의 형태학적 변화의 진단을 위한 참고자료가 될 수 있을 것으로 사료된다.

  • PDF

Replacement of the antifreeze-like domain of human N-acetylneuraminic acid phosphate synthase with the mouse antifreeze-like domain impacts both N-acetylneuraminic acid 9-phosphate synthase and 2-keto-3-deoxy-D-glycero-Dgalacto- nonulosonic acid 9-phosphate synthase activities

  • Reaves, Marshall Louis;Lopez, Linda Carolyn;Daskalova, Sasha Milcheva
    • BMB Reports
    • /
    • 제41권1호
    • /
    • pp.72-78
    • /
    • 2008
  • Human NeuNAc-9-P synthase is a two-domain protein with ability to synthesize both NeuNAc-9-P and KDN-9-P. Its mouse counterpart differs by only 20 out of 359 amino acids but does not produce KDN-9-P. By replacing the AFL domain of the human NeuNAc-9-P synthase which accommodates 12 of these differences, with the mouse AFL domain we examined its importance for the secondary KDN-9-P synthetic activity. The chimeric protein retained almost half of the ability of the human enzyme for KDN-9-P synthesis while the NeuNAc-9-P production was reduced to less than 10%. Data from the homology modeling and the effect of divalent ions and temperature on the enzyme activities suggest conformational differences between the human and mouse AFL domains that alter the shape of the cavity accommodating the substrates. Therefore, although the AFL domain itself does not define the ability of the human enzyme for KDN-9-P synthesis, it is important for both activities by aiding optimal positioning of the substrates.

9mm 합금타이타늄 중간 선재 연구 (A Study on The Φ 9mm Titanium Alloy Wire)

  • 김상연
    • 한국산학기술학회논문지
    • /
    • 제13권1호
    • /
    • pp.8-13
    • /
    • 2012
  • Ti-3Al-2.5V 잉고트를 제작하고 직경 9mm 합금타이타늄 wire로 가공하여 수입소재와 비교하여 그 특성을 조사하였다. 잉고트는 산소함량이 0.11wt%이고 철 함량이 0.085wt%로서 ASTM Gr.9 규격을 만족하였다. 9mm 합금 타이타늄 wire 경도는 225-250Hv로 비슷한 결과를 얻었고, 인장강도는 수입소재가 804MPa이고, 국내 개발품은 734MPa이었다. 연신율은 수입소재가 12%이고 국내 개발품은 22%였다. 직경 400mm 잉고트에서 단조 및 다단 열간 압연공정을 통해 직경 9.0mm 타이타늄합금선재를 제작하는 새로운 제작공정을 개발하였다.

6-(4-요오도페닐)아미노-7클로로-5,8-퀴놀린디온의 항진균작용 및 안전성 평가 (The Evaluation of Antifungal Activities and Safeties of 6-(4-Iodophenyl)amino-7-chloro-5,8-quinolinedione)

  • 유충규;윤여표;허문영;이병무;강혜영;이유진
    • 한국식품위생안전성학회지
    • /
    • 제14권1호
    • /
    • pp.55-59
    • /
    • 1999
  • 6-(4-Iodophenyl)amino-7-chloro-5,8-quinolinedione (RCK9) was evaluated for antifungal activities. The MIC values of RCK9 were determined against A. flavus, c. albicans, C. neoformans and F. oxysporium. The RCK9 showed generally potent antifungal activities against the tested fungi. Acute oral toxicity studies of RCK9 were carried out in ICR mice of both sexes. These acute oral toxicities of RCK9 had been evaluated. RCK9 were low and LD50 values were over 2,850 mg/kg in ICR mice. The genotoxicities of RCK9 had been evaluated. RCK9 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. The clastogenicity was tested on the RCK9 with in vivo mouse micronucleus assay. RCK9 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. The results indicate that RCK9 has no genotoxic potential under these experimental conditions.

  • PDF

국내 시판 Salmonella gallinarum 9R vaccine의 안전성 및 면역원성 비교 (Comparison of the safety and immunogenicity of commercial S. gallinarum 9R vaccine)

  • 황제균;이영주
    • 대한수의학회지
    • /
    • 제49권2호
    • /
    • pp.127-133
    • /
    • 2009
  • Salmonella enterica subsp. enterica serovar gallinarum (S. gallinarum) is the agent of fowl typhoid, and the 9R vaccine is a commercial live vaccine for the prevention of fowl typhoid. The aim of this study was to assess the safety and immunogenicity of different brands of S. gallinarum 9R vaccine used in commercial laying chickens in Korea. All 9R strains originated from three different brands showed the same pattern in the biochemical and serological properties, and pulsed field gel electrophoresis (PFGE) profile. But, there was a difference in rhamnose fermentaion, agglutination with Salmonella group $D_1$ antiserum and PFGE pattern between 9R vaccine strain and field S. gallinarum isolates. In laboratory and field trials for assesment of safety and immunogenicity of 9R vaccine, all of the three 9R vaccines showed the same safety in commercial laying chickens. In addition, there was a significant difference between the vaccinated and unvaccinated control groups in mortality and the re-isolation rate of the challenge strain from the tissues (p < 0.05), and no difference by the brands of 9R vaccine. The results from this study indicated that all three different brands of S. gallinarum 9R vaccine showed highly protection against mortality and organ invasion in commercial laying chickens exposed to virulent strains of S. gallinarum.

공침법에 의한 $BaTi_4O_9, Ba_2Ti_9O_{20}$$BaTi_5O_{11}$화합물의 합성 및 그의 전기적, 열적 특성 (Synthesis of $BaTi_4O_9, Ba_2Ti_9O_{20}$ and $BaTi_5O_{11}$ Compounds by Coprecipitation Method and Their Electrical and Thermal Properties)

  • 김종옥;손우창;전성용;이경희;이병하
    • 한국세라믹학회지
    • /
    • 제31권9호
    • /
    • pp.1005-1011
    • /
    • 1994
  • The three different composition of BaTi4O9, Ba2Ti9O20 and BaTi5O11 were prepared by coprecipitation process, and then the dielectric properties of these compounds were measured at low microwave frequencies. The powder showing high level of purity was synthesised by the coprecipition reaction of BaCl2 and TiCl4 where (NH4)2CO3 and NH4OH were used as a deflocculent. Followings are the result of this study: 1. The sintering temperature increased with increasing TiO2 content. 2. BaTi4O9 powder were synthesized as a single phase by this processing technique, but the resultant Ba2Ti9O20 and BaTi5O11 phase existed with Ba2Ti9O20 and BaTi5O11 phases. 3. Single phase BaTi4O9 showed high dielectric constant value of 35, high Q value of 8100.

  • PDF

Treatment of Exogenous GDF9 and BMP15 during In Vitro Maturation of Oocytes increases the Cell Number of Blastocysts in Pigs

  • Kim, Min Ju;Kim, Young June;Shim, Hosup
    • 한국수정란이식학회지
    • /
    • 제31권1호
    • /
    • pp.9-12
    • /
    • 2016
  • Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) are oocyte-specific growth factors that regulate many critical processes involved in early folliculogenesis and oocyte maturation. In this study, effects of GDF9 and BMP15 treatment during in vitro maturation of porcine oocytes upon development after parthenogenetic activation were investigated. Neither GDF, BMP15 alone nor in combination affects the number and viability of cumulus cells or the rates of oocyte maturation and blastocyst development. However, the treatment of GDF9 on porcine oocytes increased the number of trophectodermal (TE) cells of blastocysts derived from activated oocytes (P<0.05). The treatment of BMP15 increased the cell numbers of both inner cell mass (ICM) and TE cells (P<0.05). The treatment with the combination of GDF9 and BMP15 further increased the numbers of ICM and TE cells, compared with GDF9 or BMP15 treatment alone (P<0.05). In conclusion, the treatment of GDF9 or BMP15 (or both) enhanced the quality of blastocysts via the increased number of ICM and/or TE cells.

The Impact of Perceived Transparency, Trust and Skepticism towards Banks on the Adoption of IFRS 9 in Malaysia

  • JASSEM, Suaad;RAZZAK, Mohammad Rezaur;SAYARI, Karima
    • The Journal of Asian Finance, Economics and Business
    • /
    • 제8권9호
    • /
    • pp.53-66
    • /
    • 2021
  • The global financial crisis in 2008 eroded trust towards the banking industry overall. To make such institutions more transparent, the International Accounting Standard Board developed the International Financial Reporting Standard 9 (IFRS 9). After the announcement of IFRS 9, academic research has primarily focused on examining the stability of banks due to early loan-loss recognition guidelines under the new system. There appears to be a lack of understanding of how IFRS 9 has influenced institutional depositors' opinions of bank trustworthiness. Hence the goal of this study is to determine how the adoption of IFRS 9 by banks has impacted perceptions of transparency, trust, and skepticism, from the perspective of large institutional depositors. This research was conducted in the context of Malaysian banks that follow the IFRS 9 guidelines. A framework is proposed using the signaling theory, leading to the development of a set of hypotheses. The hypotheses are tested with data collected from 654 financial analysts working in Malaysian companies that are large institutional depositors. The results indicate that the adoption of IFRS 9 has led to higher levels of perceptions of bank transparency and trust, and lower levels of skepticism towards such banks.