• Journal of Food Hygiene and Safety
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• v.28 no.1
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• pp.50-55
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• 2013

Since 1 June 2012, it is prohibited to sell oilfish as a food material but there are still many illegal cases of selling oilfish as if it is tuna or grilled Patagonian toothfish. So it is absolutely crucial to construct the system to distinguish the real food material from oilfish. There are two sorts of oil fish called Ruvettus pretiosus and Lepidocybirium flavobrunneum involved in Percifomes order and Gempylidae class. 16S DNA gene region in mitochondria was selected to design the specific primers. For design species-specific primer, the theoretical experiment were performed for the sequences of R. pretiosus, L. flavobrunneum, Thunnus thynnus, Thunnus albacores, Makaira mitsukurii and Xiphias gladius, registered at the Gene bank from the National Centre for Biotechnology Information, using BioEdit 7.0.9.0. program. Through the analysis of the result from experiments, it was possible to design the 4 kinds of primers to distinguish R. pretiosus and L. flavobrunneum. As a comparison group, 3 kinds of tuna and 4 kinds of billfishes were selected and experimental verification was performed. As a result, for R. pretiosus and L. flavobrunneum, R.P-16S-006-F/R.P-16S-008-R and L.F-16S-004-F/L.F-16S-006-R primers were selected eventually and PCR condition was established. In addition, 178bp and 238bp of PCR products were confirmed from the established condition and non-specific band was not amplified among similar species. Therefore, the species-specific primers developed in this study would be very useful and used in various ways such as internet shopping mall and illegal distributions with fast and scientific results.

• Korean Journal of Ichthyology
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• v.32 no.1
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• pp.26-31
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• 2020

A single specimen of Lepidocybium flavobrunneum (910.0 mm standard length) in the family Gempylidae, was collected off northwestern Jeju Island, Korea on April 28, 2016. The species is characterized by a blackish body, very low first dorsal fin spines, second dorsal fin rays followed by six finlets, anal fins followed by five finlets, caudal peduncle with a large keel flanked by two small supplementary keels, and a lateral line with an irregular wave pattern. Here, we propose the new Korean names "Heuk-gal-chi-ggo-chi-sok" for the genus Lepidocybium and "Heuk-gal-chi-ggo-chi" for the species L. flavobrunneum.