• Journal of Applied Biological Chemistry
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• v.60 no.2
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• pp.173-178
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• 2017

This study was conducted to investigate the effect of pyroligneous acids on urea hydrolysis for the purpose of inhibiting ammonia volatilization during urea fertilizer application. Different types of synthetic urease inhibitors have been searched and developed, but their use is limited due to varying inhibition effects on soil urease, and environmental problems. In this study, the effect of pyroligneous acids, a natural substance, on urea hydrolysis in soil was evaluated by analyzing inhibition of urease activity. Pyroligneous acids inhibited plant urease and microbial urease activity, as well as soil urease with various urease complex. In addition, pyroligneous acids exhibited non-competitive urease inhibition effect through urease kinetics and inhibited urea hydrolysis in the soil. This study showed that pyroligneous acids treatment with urea fertilizer decreases the loss of urea fertilizer, improves the efficiency of nitrogen application on plant and reduces the amount of nitrogen fertilizers applied in soil.

• Asian Journal of Turfgrass Science
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• v.10 no.3
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• pp.263-270
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• 1996

This experiment was conducted to investigate the loss of various herbicides by means of vola-tility from the turfgrass field and the hare ground with the different soil moisture contents and temperatures. Different herbicides were applied at the rates of 375 g a.i. /l0a of pendimethalin,250 g a.i. /l0a of napropamide, and 96.4 g a.i. /l0a of dicamba with 200 \ulcorner/10a of spray volume in the turfgrass(Zoysia japonica cut off 5cm) grown in pots(265.8 $cm^2$) and bared soil. The pots were placed in the growth chamber with 10,000 lux of light intensity(12h per day) at 25 and 35˚C for 7days. Amberlite XAD polymeric resin(20/50 mesh) was used as sampling media for herbicide airborne residues. Air flow was maintained at 10 \ulcorner /min by vacuum pump regulated with a factory calibrated flow meter. Herbicide airborne residues were extracted from the XAD resin with 300 ml of 1:1 acetone and hexane. The extracts were concentrated by rotary evaporation at 35˚C and dissolved in 1 ml MeCN for HPLC analysis. The airborne losses of the herbicide applied in the turfgrass and bare soil increased as the temperature and soil moisture contents were increased, regardless of the kinds of herbicide. Higher airborne residues was observed in the turfgrass pots than the bare soil pots. Pendimethalin and dicamba with higher vapor pressure gave rise to the increased loss of airborne herbicides, showing that 6.26 and 6.4% of average airborne loss in pendimethalin and dicamba, respectively, compared to 0.56% in napropamide. The amount of airborne losses in turfgrass was highest at one day after application and then a declined trend was observed as the time was prolonged. Key words. Herbicides, Turfgrass field, Bare ground, Volatility.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.6
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• pp.1023-1026
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• 2011

Nitrogen content in soil is a major factor for the crop growth. Ammonium nitrogen in soils is volatilized when soil pH is high. The growth and development problem of strawberry such as color of leaves turning into purple was found when soils were treated with the compost prepared from suspended solids of high pH from the sea. From in-situ analysis it was found that soil pH was 8.33 and nitrate, phosphorous, and potassium contents were relatively low. Nitric acid was added to adjust pH of irrigating water as 1.7, 1.9, and 2.3, then $KNO_3$ $0.25g\;L^{-1}$ and $KH_2PO_4$ $0.25g\;L^{-1}$ were added. It was resulted that soils with pH 1.7 produced the most developed strawberries. Strawberry was recovered by irrigation containing the same solution. From the results, the growth and development problem of the strawberry resulted from low nitrate absorption rate. It was concluded that the growth and development of strawberries were recovered by the reduced soil pH using nitric acid.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.3
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• pp.157-163
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• 2005

The increasing of greenhouse gases may change agricultural environment. The agronomic productivity will depend upon change of temperature, precipitation, solar radiation and fertilization. Particularly, nitrogen fertilization considerably influences rice productivity and agricultural environments. This experiment was conducted to study transformation of nitrogen and to determine the primary yield components responsible for yield differences in paddy soil. $NH_4-N$ concentration of NPK plot in surface water of paddy soil was $2.07mg\;L^{-1}$ at 5 days after transplanting, and then was decreased sharply due to rice absorption and loss to environment. $NO_3-N$ concentration of NPK plot in surface water was $3.97mg\;L^{-1}$ at 10 days after transplanting. $NO_3-N$ concentration range of CRF plot in surface water was $3-5mg\;L^{-1}$ at 30th after transplanting. The accumulation of $NH_3$ volatilization in NPK plot was $22.39kg\;ha^{-1}$, which accounted for 20% of N fertilizer applied but using of CRF fertilizer can reduce $NH_3$ volatilization by 67% in paddy soil. Use efficiency of N fertilizer was not different between CRF70% and CRF100% plot. Rate of N use efficiency were 27.4%, 51.2%, 49.0% in paddy field NPK, CRF70% and CRF100% plots respectively. The yield of CRF70% showed the best effect with 9.3% increase production ratio, compare with NPK plot.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.4
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• pp.292-301
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• 2001

The objectives of this study were to measure the changes in soil moisture regimes and the distribution patterns of inorganic N derived from the fertigated $^{15}N$-labeled urea, and compare them with the results obtained from broadcast-applied soil under the same drip irrigation domain. In fertigated soil, a $^{15}N$-labeled urea solution of $117mg\;N\;L^{-1}$ was applied by surface drip irrigation for 4 weeks. In broadcast-applied soil, no the other hand, 4 g of $^{15}N$-labeled urea(1.87 g N) mixed thoroughly with 5 kg of soil was placed on the surface of packed soil. Soil water status was controlled by drip irrigation scheduled at soil matric potential of -50 kPa. A calibrated time-domain reflectometry probe was installed in the soil vertically 15 cm apart from a drip emitter to control drip irrigation. About 60% of urea-derived inorganic nitrogen was remained in the top zone between 0 and 10 cm depth of fertigated soil, while, most of the inorganic nitrogen (91%) was accumulated in the top zone of broadcast-applied soil. Of inorganic nitrogen derived from urea, the percentage of $NO_3{^-}$ was much higher for fertigation (99%) than for surface application (62%). The relatively lower recovery of urea-derived inorganic nitrogen of broadcast-applied urea-N (51%) than that of fertigated urea-N (89%) was attributable to enhanced $NH_3$ volatilization.

• The Korean Journal of Pesticide Science
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• v.20 no.4
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• pp.305-311
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• 2016

In cultivation environment, various pesticides are used and some of them could be volatilized into the air. This could affect farmer's health and also cause environmental pollution. This study was carried out to investigate the volatilization of pesticides, and use the reference data for preventing farmer's pesticide intoxication and securing worker safety. The experiment was conducted in a greenhouse using a lysimeter which was of $1m^2$ area and 1.5 m depth filled with upland soil. The pesticides treated in lysimeter soil were ethoprophos (5.0% GR), diazinon (34.0% EC), alachlor (43.7% EC), metolachlor (40.0% EC), chlorpyrifos (2.0% GR), pendimethalin (31.7% EC), carbaryl (50.0% WP), napropamide (50% WP), tebuconazole (25.0% WP) and imidacloprid (2.0% GR). Each pesticide was treated at a concentration of 770.5 mg based on A.I (%). The recovery of pesticide ranged from 77.4 to 99.3%. The volatilized pesticides in air were collected by personal air sampler with PUF tube at 4 l/min flow rate. In addition, temperature and humidity were measured. The collected samples were extracted using acetone in a soxhlet apparatus for 8 hours. The extracted pesticides were resoluted with acetonitrile and diluted 5 times. It was analyzed with LC-MS/MS. For 720 hours experiment, the largest vaporization amount of each pesticide in air was ethoprophos $15.24{\mu}g/m^3$, diazinon $5.14{\mu}g/m^3$, pendimethalin $2.70{\mu}g/m^3$, chlorpyrifos $1.76{\mu}g/m^3$, alachlor $1.40{\mu}g/m^3$, metolachlor $1.12{\mu}g/m^3$, carbaryl $0.27{\mu}g/m^3$, napropamide $0.22{\mu}g/m^3$, tebuconazole $0.11{\mu}g/m^3$ and imidacloprid $0.05{\mu}g/m^3$. The R value (coefficient of correlation) between volatilization and vapor pressure of pesticides is higher than 0.99. Therefore, there is high correlation between volatilization and vapor pressure of pesticides.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.124-130
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• 2006

Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.

• The Korean Journal of Pesticide Science
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• v.10 no.3
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• pp.172-182
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• 2006

This study was designed to assess molinate fate in the lysimeter by measuring the total radioactivity in the leachate, evolved $^{14}CO_2$, and $^{14}C$-residues in soil and rice plant. The amounts of applied $^{14}C$ in the leachate from the lysimeter for 20 weeks were 1.05% in 2.31 pore volume (217,465 mL) at the first and 0.34% in the second year, respectively. The amount of $^{14}CO_2$ evolved from the lysimeter accounted for 6.47% and 0.03% of applied $^{14}C$ in the first and second year. The $^{14}C$-activities in the soil layer of the lysimeter were distributed 18.0% (1st) and 13.3%(2nd) in the depth of 0 to 10 cm, 4.3 (1st) and 1.1% (2nd) in the depth of 10 to 20 cm. Most of the applied $^{14}C$ was detected in the top 20 cm soil layer. Total $^{14}C$ in rice plants grown at lysimeter were detected 11.46% of applied $^{14}C$. 11.11% in straw, 0.24% in brown rice grain, 0.08% in chaff and 0.03% in ears were distributed in the first year. Consequently, environmental fate of molinate using lysimeter simulating a paddy rice field were investigated 25.24% in soil, 11.64% in rice plant, 1.05% in leachate, 6.74% in evolved $^{14}CO_2$ and 0.02% in volatilized organic chemicals in the first year.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.6
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• pp.328-333
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• 2005

Ammonia volatilization is the major form of nitrogen (N) loss from flooded paddy soils and causes low N use efficiency. The effects of controlled release fertilizer (latex coated urea complex fertilizer, LCU) on reducing N loss by ammonia volatilization was measured comparing with urea in rice culture system of direct seeding on dry soil. In the treatment of urea, $NH_4-N$ concentration in surface water after flooding increased rapidly up to $8-10mg\;L^{-1}$ as affected by topdressing, while in the LCU treatment $NH_4-N$ concentration in surface water was less than $1mg\;L^{-1}$ during rice growing season. Relation of $NH_4-N$ concentration in surface water and ammonia volatilization was significant in urea treatment. The amount of ammonia volatilized from rice paddy of LCU treatment was $2.4-3.0kg\;ha^{-1}$ and the rate of ammonia volatilization from N fertilizer applied was only 2.0-2.3% compared with 5.9-7.9% in urea treatment. Therefore, N loss by ammonia volatilization could be reduced by 72-76% with by LCU compared with urea in rice culture system of direct seeding on dry soil.

• Korean Journal of Environmental Agriculture
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• v.18 no.4
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• pp.366-371
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• 1999

This study was conducted to examine the change of nitrogen in soils treated by cow manure originated from the clay mineral feeding. Bentonite, zeolite, and porphyry were feeded. CEC of the porphyry showed the lowest value, 12.02 cmol/㎏, among three kinds of clay mineral. The manure treated soils were incubated at 14, 18, 22, $25^{\circ}C$. CEC of the manure after bentonite, zeolite, porphyry feeding were 147.5, 137.0, and 114.0 cmol(+)/㎏, respectively. These values were higher than that of non-mineral treated manure, 107.5 cmol(+)/㎏. After 8 weeks incubation, there were no significant difference in the content of $NH_4-N$, but the content of $NO_3-N$ in soils were in order of zeolite treated > bentonite treated > porphyry treated > non-nimeral treated. These results suggested that the clay mineral adsorbed $NH_4\;^+-N$ and prevent the loss of nitrogen from soils.