• Journal of Pharmacopuncture
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• v.13 no.2
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• pp.67-73
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• 2010

Objective : The purpose of this study is to determine the efficacy and safely of Hwangryunhaedok-tang Pharmacopuncture (HHT-PA) therapy on post-stroke depression (PSD) in stroke patients. Methods : Fifteen PSD patients has been recruited and evaluated with liver function test (LFT) and renal function lest (RFT) and depression scales, Beck Depress ion Inventory (BDI) and Hamilton Depression Rating Scale (HAM-D) before and after HHT-PA treatment. Results : HAM-D score significantly decreased after HHT-PA treatment regard less of improving of motor function. But BDI score did not decrease significantly. All results of LFT and RFT were in normal range before and after HHT-PA treatment Conclusion : These results showed that HHT-PA decrease PSD symptoms and has safety on stroke patients, although has no effect on improving of motor impairment.

• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.87-92
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• 2006

Objective : This experimental study was performed to investigate the effect of Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma on Staphylococcus aureus that induce keratitis. Methods : MIC was measured by dropping to $50\;{\mu}l$ according to density Soyum Herbal-acupuncture, Hwangryunhaedoktang & Coptidis rhizoma(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results : 1. MIC on Staphylococcus aureus in Soyum Herbal-acupuncture disappear all sample. 2. MIC on Staphylococcus aureus in Hwangryunhaedoktang disappear all sample. 3. MIC on Staphylococcus aureus in Coptidis rhizoma was 100%, $50\;{\mu}l$ and anti-bacterial potency increase compare with quantity. Conclusions : The present author think that Soyum Herbal-acupuncture must be extracted a new method for anti-bacterial potency and Coptidis rhizoma was made Herbal-acupuncture for eye drops.

• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.99-103
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• 2006

Objective : This experimental study was performed to investigate the effect of Hwangryunhaedoktang Herbal-acupuncture & extract on Staphylococcus aureus and Staphylococcus epidermidis that induce keratitis. Methods : MIC was measured by dropping to $50{\beta}^{\circ}$ according to density Hwangryunhaedoktang Herbal-acupuncture & extract(100%, 10%, 1%, 0.1%). Anti-bacterial potency was measured by the size of inhibition zone With change of volume. Results : 1. MIC on Staphylococcus aureus in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. 2. MIC on Staphylococcus epidermidis in Hwangryunhaedoktang Herbal-acupuncture & extract were not disappear all sample. Conclusions : The present author think that Hwangryunhaedoktang Herbal-acupuncture must be extract a new method for anti-bacterial potency.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.27 no.1
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• pp.140-148
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• 2014

Objectives : The purpose of this study is to report the effect of Hwangryuhaedok-tang gagambang on seborrheic dermatitis. Methods : The clinical study was done on 3 cases of seborrheic dermatitis patients with symptoms of flare, scales, pricking and itching. An author prescribed Hwangryuhaedok-tang gagambang for these patients and checked VAS(Visual Analogue Scale) scores depending on the patient's evaluation. Photographies were taken before and after the treatment. Results : VAS scores of all patients decreased from 10 to 1,2. Judging by photographies, patients noticeably improved in facial skin. As a result of examining VAS scores and photographies, Hwangryuhaedok-tang gagambang was effective on seborrheic dermatitis. Conclusions : From these results, Hwangryuhaedok-tang gagambang may be considered as a basic prescription for seborrheic dermatitis. And an author consider that continuous clinical study will be needed in Korean medical dermatology.

• Korean Journal of Acupuncture
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• v.26 no.4
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• pp.211-223
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• 2009

초임계추출법과 수증기증류법을 이용하여 황련해독탕의 정유성분을 추출하여 성분 패턴을 비교하였다. 이때 초임 계추출조건은 압력 200 atm, $45^{\circ}C$ 그리고 추출시간 25분 이었다. 이때의 추출된 성분의 93.9%인 37개의 성분을 가스크로마토그래피/질량분석기로 확인하였다. 주요성분으로는 tetradecenoic acid (11.7%), Vanillin (5.9%), dl-Limonene (5.5%) 및 Eicosane (4.6%)으로 나타났다. 수증기증류법으로 추출한 정유에서는 34개 성분을 확인할수 있었다. 주요성분으로는 tetradecenoic acid (8.9%), Vanillin (5.8%)및 Eicosane (4.7%)를 확인 할 수 있었다. 또한 구강내의 12균주를 이용하여 항균효과를 측정하여 최소엑제농도(MIC)와 사멸농도(MBC) 0.025 - 12.8 mg/ml와 0.05 - 12.8 mg/ml 각각 나타났다.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.25 no.2
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• pp.68-75
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• 2012

Objectives : The purpose of this study is to report the effect of Hwangryunhaedok-tang Pharmacopuncture Therapy on Seborrheic Dermatitis Methods : An author treated distillation of Hwangryunhaedok-tang Pharmacopuncture(0.8cc) on both LI20(Yenoghyang), SI18(Gwollyo), ST7(Hagwan) of patient's spots on the face suitable for acupuncture and examined variation of two Seborrheic Dermatitis cases Results : The Hwangryunhaedok-tang Pharmacopuncture Therapy was effective on amelioration of symptoms like flare, pricking and itching sense in Seborrheic Dermatitis Conclusions : The above result indicate that Hwangryunhaedok-tang Pharmacopuncture Therapy has an effect on Seborrheic Dermatitis, and an author consider that continuous clinical study will be needed in other Pharmacopuncture and Korean medical treatment.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.27 no.4
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• pp.251-260
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• 2014

Objectives : The purpose of this study is to investigate the effect of eating Saengshik and applying Hwangryunhaedok-tang on skin for pregnant woman diagnosed as Pruritus Gravidarum. Methods : This case study was done on 5 Pruritus Gravidarum patients who had visited Korean Medicine Clinic. All the patients are treated with Saengshik and Hwangryunhaedok-tang was applied on their skin. VAS(Visualanalogue scale) and photographs were used to evaluate the symptom change. Results & Conclusion : Most symptoms of patients were improved in this study. Saengshik and Hwangryunhaedok-tang can be effective in patients who were diagnosed as Pruritus Gravidarum.

• The Journal of the Society of Stroke on Korean Medicine
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• v.13 no.1
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• pp.118-125
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• 2012

Object : The purpose of this study is to evaluate the effect of Hwangryunhaedok-tang herbal-acupuncture therapy for Agitation(Byun-jo) caused by cerebral infarction. Methods : One patient was admitted who was suffering from insomnia, chest discomfort, anxiety, palpitation of Agitation(Byun-jo), after cerebral infarction. In the point of Differentiation of Syndromes(辨證), we diagnosed this patient as Excessive heat-fire syndrome of stroke(中風火熱證) and treated with Hwangryunhaedok-tang herbal-acupuncture therapy at CV17, BL15, GB20. Results : After Hwangryunhaedok-tang herbal-acupuncture therapy, symtoms of Agitation(Byun-jo) were improved remarkably, and the score of State-Trait Anxiety Inventory(STAI), Pittsburgh Sleep Quality Index(PSQI) were decreased. Conclusions : Hwangryunhaedok-tang herbal-acupuncture therapy has meaningful effect on the symptoms of Agitation(Byun-jo) caused by cerebral infarction and more researches should be followed.

• Herbal Formula Science
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• v.20 no.2
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• pp.83-92
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• 2012

Objectives : The aim of this study was to evaluate the efficacy of Hwangryunhaedok-tang (HHT) and Gungangbuja-tang (GBT) on lipopolysaccharide (LPS)-induced mouse model of inflammation. HHT and GBT are one of the representative prescriptions of cold drug and one of the representative prescriptions of hot drug, respectively. For experimental evaluation of their efficacy, we investigated the anti-inflammatory effects of HHT and GBT on LPS-induced inflammation and the mechanisms of their action. Methods : ICR mice were given a HHT (50, 500 mg/kg), GBT (100, 1000 mg/kg) extract orally on three consecutive days. On the third day, they were administered LPS intraperitoneally (35 mg/kg), 1 h after the last sample administration. Blood and liver samples were taken 6 h after the LPS challenge. Cytokine expression and inflammation-related protein factor analyses were performed by Western blotting. Results : Oral administration of HHT significantly reduced pro-inflammatory cytokines, including interleukin (IL)-6, and interferon (IFN)-${\gamma}$ in the serum. While GBT inhibited an increase of IL-6, IFN-${\gamma}$ was not affected. Immunoblot analysis showed that LPS-induced NF-${\kappa}b$ activation was inhibited by GBT, meanwhile HHT only inhibited NF-${\kappa}b$ expression at high does (500 mg/kg). In addition, HHT and GBT inhibited LPS-induced phosphorylation of Erk1/2, Jnk and p38 MAPKs. GBT also significantly inhibited i-Nos and Cox-2 expression, and HHT inhibited only i-Nos expression. Conclusions : Both of HHT and GBT showed anti-inflammatory effects against LPS-induced endotoxemia. However, HHT significantly decreased inflammatory cytokine levels, such as IL-6 and IFN-${\gamma}$ more than GBT, while GBT significantly inhibited inflammatory proteins, including NF-${\kappa}b$, MAP Kinases, i-Nos and Cox-2, more than HHT. These results suggest that HHT and GBT regulate the different mechanisms of action and pathways, presumably by regulating cytokine levels (IL-6, IFN-${\gamma}$), NF-${\kappa}b$ activation, and several pro-inflammatory gene expression, although both of HHT and GBT have anti-inflammatory effects.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.24 no.2
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• pp.1-15
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• 2011

Objective : Recently interest of fermented herb medicine has increased in Oriental medicine. Herb medicine fermented in a proper condition resultingly has better or new virtue by microbe. This study was designed to compare the anti-inflammatory effects of herbal decoction and fermented herbal decoction in Hwangnyeonhaedok-tang (Huanglian fiedu-tang; HHT). Methods : The effects of HHT and FHHT on anti-inflammation were measured by production of NO and pro-inflammatory cytokine productions including TNF-${\alpha}$ and IL-1${\beta}$, IL-6 in LPS induced RAW 264.7 cells. Results : 1. Cell viability in three doses of HHT extract (0.01, 0.05 and 0.10 mg/ml) and two doses of FHHT extract (0.01, 0.05 mg/ml) was more than 70％ and 80％ each. 2. In RAW 264.7 cells, LPS displayed significant stimulatory effects on the production of NO. However, both HHT, FHHT showed significant inhibitory effects on the production of NO. 3. Pre-treatment by HHT in a various concentrations (0.01, 0.05, 0.10, 0.50mg/ml) reduced the elevated production of TNF-${\alpha}$, IL-1${\beta}$, IL-1${\beta}$ mRNA, IL-6 mRNA. More than 0.05mg/ml concentrations of HHT reduced the production of IL-6. And TNF-${\alpha}$, IL-1${\beta}$ level of mouse serum reduced in a various dose(0.1, 0.5, 1.0g/kg). 4. Pre-treatment by FHHT in a various concentrations (0.01, 0.05, 0.10, 0.50mg/ml) reduced the elevated production of TNF-${\alpha}$. More than 0.05mg/ml concentrations of FHHT reduced the expression of TNF-${\alpha}$ mRNA. 0.1, 0.50mg/ml concentrations of FHHT reduced the production of IL-6. And IL-1${\beta}$ level of mouse serum reduced in a various dose(0.1, 0.5, 1.0g/kg). Conclusions : These results indicated that both HHT and FHHT could have anti-inflammatory capacity by inhibiting the production of NO and cytokines in vitro. But HHT has more strong anti-inflammatory effects than that of FHHT in broad outlines.