• The Korean Journal of Mycology
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• v.41 no.4
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• pp.295-300
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• 2013

This study was conducted to test effect of fungicides on the spore germination and mycelial growth of BotaniGard$^{(R)}$(Beauveria bassiana GHA). Eight fungicides, which are using for plant disease control in cucumber cultivation, with different concentration were mixed with conidia of GHA isolate for 0.5, 2, and 4 hours. The fungicides trifluminazol, fenarimol, ethaboxam, copper hydroxide and tetraconazol did significantly not inhibit on spore germination and mycelial growth. The trifluminazol, propineb and tetraconazol on high concentrations inhibited the mycelial growth of GHA. However, mixing time of fungicide with conidia of GHA did not affect in spore germination and mycelial growth. These results suggest that fungicide effect needed be checked before mycopesticide is applied in the field.

• Applied Chemistry for Engineering
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• v.34 no.3
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• pp.299-306
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• 2023

In this study, the O/W emulsification processes with the natural surfactants that were extracted from Medicago sativa L. and Sapindus saponaria L. as emulsifiers were optimized using the central composite design-response surface methodology (CCD-RSM). Herein, independent parameters were the amounts of mixed emulsifiers, the mixing ratio of natural emulsifiers (soapberry saponin/alfalfa saponin), and the emulsification time, whereas the reaction parameters were the emulsion stability index (ESI), mean droplet size (MDS), and antioxidant activity (DPPH radical scanvenging activity). Through basic experiments, the ranges of operation variables for the amount of mixed emulsifiers, the mixing ratio of natural emulsifiers, and the emulsification time were 12~14 wt%, 30~70%, and 20~30 min, respectively. The optimum operation variables deduced from CCD-RSM for the amount of mixed emulsifiers, the mixing ratio of natural emulsifiers, and the emulsification time were 13.2 wt%, 44.2%, and 25.8 min, respectively. Under these optimal conditions, the expected values of the ESI, MDS, and antioxidant activity were 88.7%, 815.5 nm, and 38.7%, respectively. And, the measured values of the ESI, MDS, and antioxidant activity were 90.6%, 830.2 nm, and 39.6%, respectively, and the average experimental error for validating the accuracy was about 2.1%. Therefore, it was possible to design an optimization process for evaluating the O/W emulsion process using CCD-RSM.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2006.11a
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• pp.203-206
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• 2006

We have studied, for maximizing the total impulse of liquid propulsion system, Propellant Utilization System (PUS) to minimize outage of propellant. Propellant outage is mainly influenced by propellant mixture ratio during flight and real quantity of loaded propellant. If one employs cryogenic propellant, the variation of propellant density due to the temperature change has major effect on outage control. Feedback control of propellant level of each tank during flight could deplete both tanks simultaneously. To introduce this system, however, the mixture ratio control system of rocket engine is necessary.

• Culinary science and hospitality research
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• v.9 no.4
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• pp.1-12
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• 2003

This study was conducted to evaluating the antioxidative activities of waffle by natural antioxidants combination (tocopherol, rosemary extract and tea polyphenol) and packaging. Waffle stored at $25^{\circ}C$ and 35$^{\circ}C$ for 70 days were evaluated for acid value, peroxide value and Q$_{10}$ value. Tocopherol, either alone or with rosemary and tea polyphenol, was highly effective in inhibiting lipid oxidation of waffle at $25^{\circ}C$ and 35$^{\circ}C$. Especially among them, rosemary extract exhibited the best synergistic effect, as determined by peroxide value measurement. Waffle was then packaged under the following packing materials (OPP/DL/VMCPP, PET/DL/VMCCP). As the temperature condition, the modified atmosphere decrease in waffle package and increase peroxide value. This result indicated that the PET package was more effective in reducing oxidation than the OPP package.

• Journal of the Korean Society of Propulsion Engineers
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• v.17 no.3
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• pp.67-75
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• 2013

The numerical analysis for the verification of preburner's cooling characteristics applying to kerosene-LOx rocket engine has been fulfilled. The distribution of combustion gas properties in primary combustion zone was calculated by the mixture ratio based on head injector arrangement, the properties of oxygen flowing in wall channels as coolant were applied under real-gas conditions, and multi-phase mixing model was employed to calculate the mixing process of primary combustion zone with liquid oxygen which was used for wall cooling. The results of numerical analysis were compared with the experimental results, hence thermo-physical properties in cooling channels and a combustor could be quantitatively identified.

• Resources Recycling
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• v.26 no.2
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• pp.3-10
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• 2017

Rare earth elements with high purity are demanded for the manufacture of advanced materials. Light rare earth elements are contained in domestic monazite and Ni-MH batteries. In this paper, solvent extraction to separate the light rare earth elements from hydrochloric acid leaching solutions of these resources was discussed. A mixture of cationic and tertiary amine shows synergistic effect on the extraction of LREEs and the extent of pH decrease during extraction is reduced. The effect of solution pH on the extraction and synergism was reviewed. Acquisition of the operation data with mixer-settler on the separation of LREEs by this mixture is necessary to develop a process.

• KSBB Journal
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• v.14 no.5
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• pp.566-571
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• 1999

Lactic acid is of interested as the raw material of biodegradable polymer. In this study lactic acid was separated by reactive extraction with mixed tertiary amine extractant dissolved in 1-octanol/n-heptane. Mixed tertiary amine extractant was composed of tripropylamine(TPA) and trioctylamine(TOA). The concentration range of lactic acid is ca. 5wt% which is the concentration of lactic acid obtained from fermentation.Maximum distribution coeficient was obtained at 8:2 weight ratio of TPA/TOA and their extraction efficiencies were above 90%.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.105-105
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• 2002

염색체의 구조적 이상으로 인한 습관성 유산과 기형아의 출산을 예방하기 위해 착상전 배아에서 할구를 분석하여 정상적인 핵형을 가진 배아만을 이식하는 착상전 유전자 진단 (preimplantation genetic diagnosis, PGD)의 성공적인 임상 적용이 보고되고 있으며, 그 적용 범위가 확대되고 있다. 그러나 일반적인 간기의 핵상을 이용한 PGD에서는 형광직접보합법 probe의 제약으로 보인자와 정상적인 핵형을 구분할 수 없는 단점이 있다. 따라서 본 연구에서는 보다 정확한 PGD를 위해 생쥐 배아를 이용하여 분리한 할구에서 중기 염색체상을 획득하기 위해 미세소관 (microtubule) 형성 저해제를 처리하였으며, 이를 통해 확립된 방법을 인간의 PGD에 적용하고자 하였다. 과배란이 유도된 ICR 생쥐에서 4- 또는 8-세포기 배아를 수획하여 colcemid, nocodazole, vinblastine을 각각 0.1, 0.5, 1.0, 5.0$\mu$M을 처리하고, hoechst 33342로 염색하여 핵상을 관찰하여 최적의 농도를 결정하였다. 또한 각 미세소관 형성 저해제를 혼합 처리하여 가장 높은 중기 염색체상을 획득할 수 있는 혼합 처리를 결정하였다. 이렇게 결정된 혼합 처리 방법을 인간의 체외 수정 및 배아 이식술에서 획득된 3PN 배아에 처리하여 중기 염색체를 획득하였다. Colcemid, nocodazole, vinblastine 모두 1 $\mu$M이 최적 농도임을 확인할 수 있었다 (각각 96.3%, 92.0%, 98,4%). 미세소관 형성저해제를 혼합 처리하였을 경우 nocodazole과 vinblastine (각각 1$\mu$M)을 혼합 처리했을 때 중기 염색체 획득률(97.3%)이 가장 높았다. 인간의 3PN 배아에 1$\mu$M의 nocodazole과 vinblastine을 혼합 처리한 후, 113개의 할구를 분석하여 44개(38.9%)의 할구에서 중기 염색체를 확인할 수 있었다. 본 실험 결과를 통해 중기 염색체를 획득하기 위하여 미세소관 형성 저해제를 처리하는 방법은 생쥐의 배아에서는 효과적이지만, 인간의 배아에서는 그 효율이 다소 낮음을 알 수 있었다. 그러나 이 방법을 개선하여 인간의 할구에서 중기 염색체의 획득률을 높이고, 이를 염색체의 구조적 이상에 대한 착상전 유전자 진단에 적용한다면, 보인자와 정상의 핵상을 구분하여 정상의 핵상만을 갖는 배아의 이식을 통하여 더욱 정확한 착상전 유전자 진단을 시행할 수 있으리라 사료된다.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1990.11a
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• pp.1-15
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• 1990

엔진 성능의 향상에 맞추고 에너지 절약을 위한 방법으로써 엔진 오일의 마찰 마모 특성등 오일의 성능을 높이려는 노력이 꾸준히 시도되고 있다. 특히 마찰력 손실을 감소기키기 위해서 낮은 마찰특성을 갖도록 몰리브데늄(Mo)을 유용성인 금속 착화합물로 만든 Mo-DTP를 제조하여 현재 첨가제로 널리 사용되는 Zn-DTP와 대체시키려는 연구가 활발하고 최근엠 많은 논문이 발표되고 있다. 또 일부 윤활유 제조 회사에서는 몰리브레늄을 첨가 시키는 등 제품 기술을 향상시켜 오일 교환의 기간이나 자동차 주행 거리를 대폭적으로 늘려줄 수 있는 상품도 생산되고 있다. 윤활 첨가제로써 Mo-DTP를 Zn-DTP와 대체시켜 기유에 혼합한 경우에는 마찰 감소 기능이 매우 향상 되었고 내 마모성도 비교적 좋으나 산화 억제 능력이 부족하고 온도의 상승에 따른 점도 지수의 증가도 비교적 크다는 단점을 갖고 있음이 발표된바 있다. 따라서 Mo-DTP의 단점을 보완하려는 연구가 필요되고 그의 한 방법으로써 현재 첨가제로 가장 많이 사용되는 Zn-DTP와 혼합하여 첨가 하였을 경우에 대한 연구를 하였다. 이때 기본 윤활유에 Mo-DTP와 Zn-DTP를 적당한 혼합 비율로 첨가하고 하중, 온도를 변화 시키면서 마찰, 마모등의 성질고 산화 안정성 및 마모 입자 분석 등 전반적인 윤활유로써의 특성을 살펴 보았다.

• Journal of Veterinary Clinics
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• v.25 no.5
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• pp.330-339
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• 2008

Ceramide, cholesterol and free fatty acids are the major intercellular lipids, maintaining the integrity of the skin barrier. However, the roles of these lipids in canine skin barrier function are little known. The aim of this study was to evaluate the repairing effects of 2% ceramide (CER), 2% cholesterol (CHO), 2% linoleic acid (LIN) and 2% intercellular lipid mixture (ILM) on damaged canine skin barrier by 1.25% sodium lauryl sulphate (SLS). Transepidermal water loss (TEWL), skin hydration, skin pH and skin thickness were assessed. Histological profiles and transmission electron microscopic (TEM) profiles were assessed on day 12. SLS effectively induced the canine skin barrier damage. TEWL was significantly decreased by topical application of CER and ILM in SLS and vehicle-treated skin on day 8 and 12, respectively (p < 0.05, p < 0.0 I). By end of the experiment all lipids significantly decreased the TEWL as compared with SLS and vehicle control, but CER and ILM more significantly decreased the TEWL than UN and CHO, respectively (p < 0.01). Skin hydration was significantly increased by CER and ILM during experimental periods (p < 0.01). Skin pH was significantly decreased by CER, LIN and ILM. In histological profiles, the thickness of the stratum corneum (SC) was significantly increased by the SC lipids as compared with vehicle and SLS (p < 0.01). Especially, CER and ILM showed more prominent improvement of barrier recovery. In TEM of the SC, SLS induced exfoliations of corneodesmosomes in the SC, and CER and ILM effectively protected exfoliations of corneodesmosomes on SLS-damaged canine skin. These results indicated that topical application of CER and ILM dramatically improved damaged-skin barrier function by SLS. Also, it was considered that the use of CER or ILM was recommended for the management of skin barrier dysfunction by irritant and inflammatory skin disorders such as atopic dermatitis.