• 대한세포병리학회:학술대회논문집
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• 1992.11a
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• pp.16.1-16.1
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• 1992

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.95-96
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• 2003

본 연구에서는 VSV-G(vesicular stomatitis virus G glycoprotein)로 포장된 MoMLV(Moloney murine leukemia virus) retrovirus vector system을 이용하여 GFP가 발현되는 형질전환 닭을 생산하고자 하였다. GFP 유전자를 retroviral vector 내의 RSV(Rous sarcoma virus) promoter의 조절 하에 도입한 후, Gp293 세포주에서 virus 형태로 생산하였으며, 이 virus를 초원심분리로 고농축하여 stage X 계란의 배 반엽 층에 주입하여 GFP가 발현되는 형질전환 닭을 생산하였다. 생산된 닭에서의 GFP의 발현은 epifluorescence stereomicroscope를 이용하여 확인하였다. 이 방법은 기존의 여러 형질전환 가금 방법에 비하여 기술적인 용이성과 경제성을 가지므로(Muramatsu, Park and Okumura 1998), 매우 효율적이고 주목할 만한 형질전환 가금 생산 방법으로 사료된다. 형질전환 가금의 생산에서 retrovirus vector를 이용하는 방법은 다양한 종류의 표적세포에 대하여 retrovirus 고유의 감염성에 의한 외래 유전자의 전이가 용이하고, 전이된 유전자가 진정염색질 영역 내로 선택적으로 도입될 수 있으며 유전적으로 안정성을 나타내므로 매우 효과적인 방법이다. 그러나 가금에서는 초기 배 발달에 의한 급격한 세포의 수적 증가로 인해 고감염성 virus의 획득이 요구되므로, 본 연구에서는 virus의 농축에 있어 보다 안정적이고 숙주 범위에 있어서 pantropic한 VSV-G에 기반을 둔 retrovirus vector system을 확립하였다. 이 system은 기존의 형질전환 닭의 생산방법에 비해 외래 유전자의 전이에 있어서 매우 효과적인 것으로 확인되었으며, 또한 여러 유용한 생리활성물질을 분비하는 형질전환 동물의 생산에 있어서 상당한 기여를 할 것으로 사료된다.

• The Microorganisms and Industry
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• v.17 no.1
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• pp.29-31
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• 1991

B 세포이 가장 중요한 기능은 항체를 생산하여 체액성 면역반응을 조절하는 일이다. B 세포가 항체를 생산해 내는 형질세포(plasma cell)로 분화하기 위해서는 다음의 몇 가지 단계를 거쳐야 한다. 즉 resting 상태에서 mitogen이나 항원의 자극에 의한 활성화(activation)가 첫번째 단계이고 두번째로는 증식(proliferation)이며 마지막 단계가 분화(differentiation)이다. B 세포가 일단 mitogen이나 항원에 의해 활성화가 되면 활성화 된 clone의 증식과 분화는 T 세포에서 생산되는 여러 종류의 림포카인(lymphokine)의 영향하에서 이루어진다. 따라서 B 세포 기능에 영향을 미치는 림포카인의 종류를 알아내고 최근 새롭게 발견되는 림포카인의 B 세포에 대한 영향을 연구함이 중요하리라 생각된다. 여기에서는 B 세포 기능에 영향을 미치는 여러 림포카인의 종류의 그 기능에 관해서 간략하게 기술하고자 한다.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.42-42
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• 2003

복제기술은 기존의 형질전환 동물 생산의 효율을 향상시킬 수 있는 기술로서 인정하고 있으며 또한 이를 이용하여 형질전환 동물의 생산이 이루어지고 있다. 따라서 본 연구는 표지유전자 (GFP)와 유용유전자 (hFSH)를 이용하여 임신 45일령에 채취한 태아섬유아세포에 transfection 하고, transfection 된 세포의 효율적인 선발과 이를 이용한 형질전환 복제 수정란을 생산하고자 실시하였다. 대조구 (KbFF), GFP (79KbFF-GFP c-3) 및 hFSH (79KbFF-hFSH n-1)에 공시한 세포는 모두 동일한 태아유래의 세포 (모 79, 부 KPN178,♂)를 이용하였다. pAB-eGFP와 hFSH 유전자는 각파 electroporation 방법을 이용하여 transfection 하고, 이를 2주 동안 G418로 배양하며 selection 하였다.

• Korean Journal of Animal Reproduction
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• v.27 no.1
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• pp.25-34
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• 2003

Human thrombopoietin (hTPO) is a cytokine that plays a central role in megakaryopoiesis. To direct hTPO expression in the mammary gland, an expression vector was constructed by combining the promoter of bovine beta-casein gene, cDNA of hTPO and neomycin resistance gene (pBT-L neo). Fibroblast cells derived from cow's ear skin tissue were transfected with the expression vector (pBT-L neo) using Lipofectamine. Transfected cells resistant to G418 trea？nt were cultured to form the colonies for more than 2 weeks. The transformed colonies identified by PCR were further expanded prior to nuclear transfer. Reconstructed oocytes with transformed cells were electrofused, activated using calcium ionophore and 6-DMAP, and cultured in vitro for 7 days. Of 35 cell colonies analyzed by PCR, 29 colonies (82.9%) were positive for the hTPO gene. Cleavage and developmental rates to the blastocyst stage of reconstructed embryos with the transformed cells were 65.1% and 23.8%, respectively Of 29 blastocysts that developed from reconstructed embryos with the transformed cells, 27 embryos (93.1%) were transgenic. These results indicate that transgenic bovine embryos can be efficiently produced by somatic cell nuclear transfer using transformed cells.

• Korean Journal of Clinical Laboratory Science
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• v.54 no.1
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• pp.68-72
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• 2022

Angioimmunoblastic T-cell lymphoma (AITL) is a lymphoproliferative disorder of mature T follicular helper cells. Atypical lymphoid cells were observed in the bone marrow of an 80-year-old woman, and the flow cytometric determined immunophenotypes of B-cells were unusual, that is, CD19+, CD20-, and CD22- with lambda light chain restriction. Initially, we suspected BM involvement of B-cell lymphoma based on the presence of abnormal B-cells. However, the patient was diagnosed with AITL involving BM. A re-analysis of flow cytometric immunophenotyping revealed a minor, aberrant T-cell population, and the lambda light chain restriction observed by surface staining was considered non-specific binding. This case demonstrates B-cells in patients with EBV-positive T-cell lymphoma may exhibit immunophenotypes resembling those of plasma cells, and that proliferation of abnormal B-cells or plasma cells could also potentially mask underlying T-cell lymphoma. A more integrated approach is required for accurate diagnosis.

• Journal of the Korean Society of Radiology
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• v.83 no.4
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• pp.924-930
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• 2022

Most spinal meningiomas have an intradural or partly extradural location. The meningothelial origin is the most common pathologic type of spinal meningioma. Pure extradural spinal meningiomas are not common, and lymphoplasmacyte-rich meningioma (LPRM) is very rare. We report a case of isolated extradural spinal meningioma in the thoracic spine that was pathologically confirmed as LPRM.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.309-312
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• 2002

Exogenous glutamine synthetase (GS) was used efficiently as a selectable marker to identify successful transfectants for the production of erythropoietin (EPO) in Chines hamster ovary (CHO-K1) cells in the absence of glutamine. Inclusion of methionine sulphoximine (MSX), an inhibitor of glutamine synthetase, enabled further selection of clones with relatively high levels of transfected glutamine synthetase and EPO genes which were coupled together. In this study, a new cell line was established by using GS system and enhancement of EPO production by zinc ion was evaluated using the transfected CHO-K1 cell line under normal condition. It was found that EPO production from CHO-K1 cells was enhanced 40% when the optimal amount of zinc ion was added.

• Applied Microscopy
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• v.3 no.1
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• pp.23-28
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• 1973

The changes of rough surfaced endoplasmic reticulum of plasma cells in the regional lymph node after injection of Bovine Gamma Globulin summarized as follow: The rough ER of plasma cells before antigenic stimulation shows flattened sac but changed to vesicular at 3rd day and vacuolar at 7th to 10th day. lntradisternal granules are appeared within the lumina or rough ER at 7th to 10th day. The rough ER is changed to distended sac or flattened sac at 20th day. The results suggest that normally flattened sac of rough ER is changed to vesicular and then to vacuolar after antigenic stimulation. But they are returned to distended sac or flattened sac with disappearance of antigenic stimuli.

• The Journal of the Korean bone and joint tumor society
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• v.15 no.1
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• pp.75-80
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• 2009

Extramedullary plasmacytoma is extremely rare, constitute 3 to 5 % of plasma cell malignancies and commonly occur in the upper aerodigestive tract. Several case studies of extramedullary plasmacytoma occurring in unusual location are reported; stomach, bladder, central nervous system, breast, thyroid, testis, salivary gland and skin. Here, we present a case of an extramedullary plasmacytoma of the right gluteus maximus muscle in a 49-year-old man.