• The Safety technology
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• no.204
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• pp.18-20
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• 2014

1978년 정부는 혈액의 자급자족 달성과 상업적 이용 배제라는 구제사회의 권고를 수용하여 혈장분획제제*의 국내 수급을 대한적십자사로 일원화한다는 기본 시책을 수립하였다. 이후 대한적십자사는 혈장분획제제 사업을 본격적으로 추진, 1991년 충북 음성군 감곡면에 혈장분획센터를 건립하고 제품을 생산 공급하기 시작했다. 그로부터 23년 동안 센터는 ▲혈장분획제제의 생산 및 공급 ▲원료혈장의 보관 ▲혈애검체 보관 ▲수입 혈장 확인 감독 ▲혈장 분획제제의 수입과 공급 등 주요 업무를 차질 없이 수행하며 국가보건인프라의 중추로 거듭났다. 이곳이 오랜 기간 동안 흔들림 없이 막중한 임무를 수행할 수 있었던 배경에는 '철저한 안전관리'가 자리 잡고 있다. 빈 틈 없는 시설점검과 임직원의 투철한 안전의식으로 국민보건을 책임진다는 혈장분획센터를 찾아가 보았다.

• Korean Journal of Biological Psychiatry
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• v.4 no.1
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• pp.84-94
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• 1997

This study was done to examine changes of plasma homovanillic acid(HVA), 5-hydroxyindoleacetic acid(5-HIAA), and HVA/5-HIAA ratio during an 8-week clozapine trial and to investigate the relationship between the plasma monoamine metabolites and treatment responses. Twenty-seven chronic schizophrenic patiens were treated for 8 weeks with clozapine. The psychopathology was assessed at baseline just clozapine trial and then every 2 weeks until the end of 8-week clozapine treatment using the Positive and Negative Syndrome Scale(PANSS) and the Clinical Global Impression scale(CGI). The plasma HVA and 5-HIAA levels were measured also biweekly using high preformance liquid chromatography with electrochemical detection method. Plasma HVA and 5-HIAA levels were significantly decreased during a 8-week clozapine treatment, although plasma HVA/5-HIAA ratio showed no significant change. The changes of plasma HVA levels were in significant correlations with the changes of PANSS positive scores, of general psychophathology scores, and changes of total socres. The changes of plasma 5-HIAA levels were in signfificant correlations with the changes of PANSS negative scores. But the changes of plasma HVA/5-HIAA ratio had no significant correlation with any PANSS subscale score changes. 48% of the patients treated with clozapine was categorized as responders, who showed at least a 20% decrease in PANSS total socre and a CGI severity score of mildly ill or less(${\leq}3$) at the end pint of the study. The baseline plasma HVA levels and HVA/5-HIAA ratio were significantly higher in responders(N=13) than in nonresponders (N=14). But no significant difference in baseline levels of plasma 5-HIAA was found between responders and nonresponders. At the end point of the study, there was significant difference in the change of plasma HVA between responders(40.3% decrement) and nonresponders(3.1% increment). But no signficant differences in the change of plasma 5-HIAA and the change of plasma HVA/5-HIAA ratio between responders and nonresponders were observed. These results suggest that the antipsychotic effect of clozapine on positive symptoms may be associated with dopaminergic blocking activity, and that on negative symptoms may be associated with serotonergic blocking activity. The baseline plasma HVA levels and the change of HVA levels from baseline may be useful predictors of treatment response with clozapine.

• Korean Journal of Food Science and Technology
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• v.31 no.2
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• pp.416-420
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• 1999

The effects of GOD and yeast on glucose content and pH of broiler and porcine blood plasma were investigated. The initial glucose concentration of broiler and porcine blood plasma were $150mg/100cm^{3}\;and\;143mg/100cm^{3}$, respectively. Addition of GOD and yeast decreased glucose contents in broiler and porcine plasma. As expected, plasma glucose content decreased as incubation time increased. While 1080 and 1110 min were required to remove glucose from both broiler and porcine plasma at GOD 5 units/g and 480 and 1020 min were required at GOD 10 units/g, respectively; both required 240 min at 0.3% yeast (w/w). The Maillard reaction can be prevented by desugarization. During the removal of glucose, pH of the plasma decreased. As glucose content in plasma leveled off, the pH value of plasma increased. Therefore, pH may be used as an index of desugarization.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.658-664
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• 1999

Broiler and porcine blood plasma were desugarized by GOD (glucose oxidase 10 units/g) or baker's yeast (0.3% w/w) and dried. The color, biuret protein content, solubility, foaming capacity and pH of desugarized blood plasma powder during storage at room temperature were investigated. Desugarized plasma powder was lighter and less red and yellow than the control group (P<0.05). Biuret protein content and solubility of deglucosed plasma powder were higher than the control. Biuret protein content and solubility of all samples decreased during storage (P<0.05). Generally, deglucosed samples showed better foaming capacity than the controls (P<0.05). The pH of deglucosed broiler samples by yeast and porcine samples were decreased just after initial increasing, while the pH of other broiler powder was continuously decreased during storage. Deglucosed porcine powder always showed higher pH values than the control (P<0.05). Overall, desugatization of broiler or porcine blood plasma before drying improved color, biuet protein content, solubility and foaming capacity.

• The Korean Journal of Blood Transfusion
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• v.23 no.2
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• pp.145-151
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• 2012

배경: 채혈 후 제제 경과시간에 따른 동결혈장의 혈액응고인자의 활성도와 이에 영향을 미치는 요인을 분석하여, 혈액응고인자제제의 원료혈장의 사용범위를 확장 가능한지 확인하고자 동결혈장의 채혈시간과 응고인자 특성을 측정하였다. 방법: ALT 부적격 혈장을 채혈 후 동결시간의 특성에 따라 4단계로 구분하였고, 6종류의 혈액 응고인자 활성도와 혈액형을 검사하였고, SAS 9.2 프로그램을 사용하여 통계처리 하였다. 결과: 혈액제제간 FVIII 활성도를 분석한 결과 PL-A>FFP>FP(8-24)${\approx}$FP(24-72) 순으로 유의하게 낮아졌고 혈액형에 따라서는 AB형이 제일 높고, O형이 제일 낮았다. 대한적십자사의 원료혈장에 대한 FVIII 활성 품질기준을 적용할 경우 PL-A, FFP와 FP24는 각각 85.0%와 82.5%로 적합하였다. 캐나다 퀘백 주처럼 FP24의 FVIII 활성이 0.52 IU/mL 이상을 적용할 경우 PL-A, FFP와 FP24는 각각 95.0%, 96.3%, 82.6%로 적합하였다. 또한 FP(8-24)의 A형과 AB형, FP(24-72)의 경우 AB형이 각각 82.1%, 83.3%, 100%로 적합하였다. 결론: 혈액응고인자제제용 원료혈장의 범위는 외국의 기준에 비추어 채혈 후 24시간 내에 동결된 혈장(FP24)으로 확대 사용이 가능하다. 이를 위해서는 채혈 후 동결시간과 혈액응고인자에 대한 품질기준을 유럽약전 또는 WHO 가이드라인과 비교하여 완화하는 것이 필요하다.

• Journal of Nutrition and Health
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• v.19 no.5
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• pp.296-303
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• 1986

Rat lipoprotein-deficient plasma possessed a lipid tramsfer inhibitory activity when it was added to purified human plasma lipid transfer protien, while it lacked a lipid transfer activity. Incubation of whole rat plasma with partially purified human lipid transfer protein resulted in big changes in lipid distribution of rat plasma lipoproteins. There w was a 4-fold increase in cholesteryl ester(CE) and 4 47 % reduction in triglyceride(TG) in very low density lipoproteins after 2싹lour incubation. In high density lipoprotein $2(HDL_2)$ there was a 9­fold increase in TG and 33 % reduction in CEo HDL3 had 82 % reduction in CE. The result indi­c cates that the absence of the lipid transfer activity in rat plasma can be ascribed not to the inability of rat lipoproteins to serve as substrates but to the lack of 야Ie lipid transfer protein in rat plasma. Th­e erefore, species differences in lipid transfer betwe­e en lipoproteins should be taken into consideration to interpret results of studies on lipoprotein m.eta­b bolism using rats.

• The Korean Journal of Zoology
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• v.20 no.4
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• pp.169-177
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• 1977

Plasma albumin and hemoglobin were purified from the blood of Rhabdophis tigrinus. Both purified proteins and R. tigrinus crude plasma were injected into rabbits. The resulting antisera were tested for reactivity with plasma albumin and hemoglobin from, eight species of squamate and four species of non-squamate-vertebrate. Reactivity was detected qualitatively by immunodiffusion tests and immunoelectrophoreses. Antisera against plasma albumin and crude plasma reacted only with R. tigrinus plasma albumin. No antigen-antibody reactions were detected in plasma albumins of other species. Antiserum against R. tigrinus hemoglobin reacted strongly with eight squamate hemoglobins. It is likely that the reptilian plasma albumin has been experiencing rapid structural change and the reptilian hemoglobin molecules seem to be of high homogeneity. Thus, by immunological evidences, the structure of the hemoglobin molecule is considered to have been changing at slower rate than that of the plasma albumin in reptiles.

• Journal of the Korean Society of Food Science and Nutrition
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• v.18 no.2
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• pp.195-198
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• 1989

The water binding capacity (WBC) of hoe plasma protein was investigated. The centrifugal condition for optimal separation of plasma from hog blood was fixed at 1400 g-force. The WBC of 5%-plasma-protein-solution eel increased rapidly between pH 6 and 7 but gradually after pH 7 at $85^{\circ}C$ for 30 min. The higher heating temperature demonstrated the higher WBC of 5%-plasma-protein-solution gel at pH 7 within short period of time. The WBC of 5%-plasma-protein-solution gel increased rapidly at the beginning of heating. The WBC per gram of plasma protein at pH 7 and $85^{\circ}C$ for 30 min decreased as protein concentration of the plasma solution increased.

• Korean Journal of Psychosomatic Medicine
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• v.4 no.2
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• pp.207-214
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• 1996

The study was designed to find out the effect of exam stress and vitamin B complex on hormones such as plasma ACTH, cortisol and prolactin. 21 medical students completed the whole period of the study. Global assessment of recent stress(GARS) scale and SCL-90-R were used to measure stress perception and psychopathology. Radioimmunoassay was used to assess plasma ACTH, cortisol and prolactin. Plasma ACTH level was significantly higher 2 weeks prior to examination and exam period, respectively, than 4 weeks prior to the exam. However, there were no significant differences in plasma cortisol and prolactin level among the three periods. No significant differences were also found in plasma ACTH, cortisol and prolactin level between vitamin and non-vitamin groups during each period. Scores of stress perception in economic area significantly had a positive correlation with plasma ACTH and prolactin level, respectively, 2 weeks prior to the exam. In psychopathology, scores of hostility subscale significantly had a Positive correlation with plasma ACTH level. There were no significant differences in change of each of the hormones over time as well as between vitamin and non-vitamin groups. In conclusion, it was found that ACTH was more sensitive to exam stress than cortisol or prolactin, and that vitamin B complex had no significant influence on ACTH, cortisol and prolactin level.

• Korean journal of food and cookery science
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• v.15 no.2
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• pp.191-196
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• 1999

The standard cooking method of Soondae, a famous traditional sausage type food in Korea, was proposed and the applicability of porcine plasma and egg white as the substitute of the porcine blood was investigated for the improvement of off-flavor, rancidity, and acceptability of Soondae. Sensory evaluation suggested Soondae prepared with 15% porcine blood most preferred. The cooking yields, water absorption capacity and hardness of plasma and egg white added Soondae were decreased compared with those of original porcine blood Soondae. The overall results of sensory evaluation suggested the possibility of using porcine plasma or egg white as a substitute for the porcine blood in Soondae.