• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.159-164
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• 2014

In this study, functionality of products of silkworm hemolymph fermented by Bacillus species was studied such as cell viability, antioxidant effect, and inhibitory effect on tyrosinase activities. A matured silkworm hemolymph was degraded by fermentation with Bacillus subtilis 10854 and Bacillus amyloliquefaciens M27. Especially, proteins of matured silkworm hemolymph were degraded to 3,000 Da by fermentation with B. amyloliquefaciens M27. Cell viability for MTT assay was higher than PBS in hemolymph and fermented hemolymph. A DPPH free radical scavenging activity of fermented hemolymph was as higher as Vitamin C and dependent on sample concentrations. Thus, these results suggest that degraded hemolymph fermented by B. amyloliquefaciens M27 may have antioxidant properties as a material for cosmetics.

• Journal of Sericultural and Entomological Science
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• v.51 no.2
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• pp.207-210
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• 2013

In this study, matured silkworm hemolymph was studied for inhibitory effect on melanin formation, tyrosinase activities. A matured silkworm hemolymph mass purification method was developed and some of suppressing melanin formation effects were investigated, too. As a result, the advanced purification method was higher in yield rate than existing one by 53%. A free radical scavenging activity of hemolymph was higher than sericin on the market($IC_{50}$, $202.9{\pm}33.1$) and lower than vitamin C($0.57{\pm}0.02$) by $6.4{\pm}0.3{\mu}g/m{\ell}$. An inhibitory effect on melanin synthesis was higher in hemolymph compared to Morus bark and arbutin by 9.15% and 11.56%, respectively. An inhibitory effect on tyrosinase activity was higher in hemolymph than Morus bark and lower than arbutin. Effect of hemolymph $45{\mu}g/m{\ell}$ on suppressing of tyrosinase was almost 90%. Thus, these results suggest that hemolymph of matured silkworm may have beneficial properties as a material for cosmetics.

• Korean journal of applied entomology
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• v.31 no.4
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• pp.329-337
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• 1992

Studies were carried out to investigate changes of lipid and carbohydrate contents in the hemolymph of the Oriental tobacco budworm(Helicoverpa assulta (Guenee» adults during flight and hormonal effects on mobilization of energy sources in the hemolymph. During a few minutes after flight, both sexes showed a rapid increase in lipid content and the high level was maintained for about 2 hours. But carbohydrate content in the hemolymph during flight showed almost no change but a slight increase seen during the first 10 min of flight in males only. Synthetic adipokinetic(Lom-AKH- n), hypertrehalosemic(Bld-HrTH) hormones and brain/ corpora cardiaca extract of H. assulta adult elevated lipid and carbohydrate contents in hemolymph and the effect was much more pronounced for lipid. These results suggested that lipid is a main fuel for flight activity and lipid mobilization is under the hormonal control. And this study showed that both adipokinetic and hypertrehalosemic factors may exist in H. assulta and these factors may have similar structures to those of Mas-AKH, Hez-HrTH, Lom-AKH- n or Pea-HrTH.

• Journal of Life Science
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• v.31 no.4
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• pp.425-429
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• 2021

We have investigated the expression of early growth response protein 1 (EGR1) in INS-1 pancreatic β-cells treated with Allomyrina dichotoma hemolymph. The Korean rhinoceros beetle, A. dichotoma (Coleoptera: Scarabaeidae), is important in the insect industry for medical applications. We have already established a method for purification of A. dichotoma hemolymph that can be used in many experiments. EGR1 is reported as a multifunctional transcription factor that is implicated in virus infections. EGR1 has therefore been revealed as a major mediator and regulator in the physiological and pathological conditions of several cell and tissue types. New findings in this study are that A. dichotoma hemolymph, which promotes a dose- and time-dependent upregulation of EGR1 gene expression, shows an enhancement of this gene expression when combined with hypothermia or endoplasmic reticulum (ER) stress. These results suggest that A. dichotoma hemolymph may provide clues to EGR1-associated disease therapies involving gene regulation of EGR1.

• Korean journal of applied entomology
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• v.25 no.4 s.69
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• pp.215-220
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• 1986

Glucose, trehalose, and glycogen content were measured in haemolymph and the body during larval development of the oriental corn borer, Ostrinia furnacalis ($Guen\'{e}e$). Glucose content varied in haemolymph, but in the body it gradually decreased at all larval stages. As the larva proceeds growth, the initial high level of trehalose increased further in haemolymph whereas the low level trehalose gradually decreased in the body. Glycogen levels in haemolymph rose gradually during larval growth whereas those in the body increased sharply at the two latter instar larval stages. It is apparent that the existence of a homeostasis in carbohydrate levels between haemolymph ana the body.

• Journal of Chest Surgery
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• v.39 no.5 s.262
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• pp.423-425
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• 2006

A case report of lymphangiohemangioma of the mediastinum that was misdiagnosed as thymic origin mass on chest CT and MR angiography. Operative finding revealed vascular proliferation originated from innominate vein and the pathologic finding showed both lymphatic and vascular component which was diagnosed lymphangiohemangioma.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 1998.05a
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• pp.29-29
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• 1998

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.183.2-183
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• 1994

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1046-1052
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• 2007

Transferrin is a molecule carrying iron to store and maintain for iron homeostasis of living organisms. In this study, we have purified transferrin, as an iron-binding protein, from the last larval haemolymph of Papilio xuthus by KBr density gradient ultracentrifugation and gel filtration (superose 6 HR) using fast protein liquid chromatography (FPLC) and transferrin containing iron was identified by Ferene S staining. The purified haemolymph transferrin was shown to have molecular mass of 78 and 80 kDa and amino acid composition of transferrin was rich in aspartic acid, valine, leucine and glutamic acid. With immuno-diffusion assay, we confirmed the existence of the transferrin in the haemo-lymph and fat body by detection of visible and clear positive reaction. From the quantitative comparison by rocket immuno-electrophoresis process, the amount of transferrin were increased in the haemolymph of 3 days after pupation and the whole 5 days after pupation. Here, with biochemical and immunohistochemical analysis, we speculate the relationship of transferrin between the physical characteristics and distribution during metamorphosis of P. xuthus.

• The Korean Journal of Zoology
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• v.23 no.1
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• pp.1-12
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• 1980

Changes and possible origin of haemolymph proteins during the cuticle formation and hardening are determined by means of acrylamide gel electrophoresis and immunodiffusion. The results by acrylamide gel electrophoresis showed at least 19 protein bands in the haemolymph and 13 fractions in the fat body with relatively constant pattern during the period of cuticle formation and hardening. Both haemolymph and fat body proteins are generally characterized by the presence of three to four heavy stained bands and several thin bands near the top region of the gel. At least over five haemolymph proteins are constantly present during this period. Immunodiffusion tests show that of total eight to nine pupal haemolymph proteins two proteins were already detected in the fat body before pupation and other two proteins were also found in the fat body immediately after pupation, suggesting fat body as possible source of these two haemolymph proteins.